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However, the underlying mechanism of its action on aquatic products is not completely understood. Methods: A high-fat diet (HFD)-induced NAFLD zebrafish model was successfully established, and the anti-hyperlipidemic effect and potential mechanism of \u03b2-sitosterol were studied using oil red O staining, filipin staining, and lipid metabolomics. Results: \u03b2-sitosterol significantly reduced the accumulation of triglyceride, glucose, and cholesterol in the zebrafish model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differential lipid molecules in \u03b2-sitosterol mainly regulated the lipid metabolism and signal transduction function of the zebrafish model. \u03b2-sitosterol mainly affected steroid biosynthesis and steroid hormone biosynthesis in the zebrafish model. Compared with the HFD group, the addition of 500 mg\/100 g of \u03b2-sitosterol significantly inhibited the expression of Ppar-\u03b3 and Rxr-\u03b1 in the zebrafish model by at least 50% and 25%, respectively. Conclusions: \u03b2-sitosterol can reduce lipid accumulation in the zebrafish model of NAFLD by regulating lipid metabolism and signal transduction and inhibiting adipogenesis and lipid storage.<\/jats:p>","DOI":"10.3390\/ani14091289","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T08:08:32Z","timestamp":1714032512000},"page":"1289","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["\u03b2-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6231-9162","authenticated-orcid":false,"given":"Peng","family":"Zhang","sequence":"first","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naicheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3370-5712","authenticated-orcid":false,"given":"Mingyang","family":"Xue","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zidong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5075-7154","authenticated-orcid":false,"given":"Chen","family":"Xu","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7291-2493","authenticated-orcid":false,"given":"Yuding","family":"Fan","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6416-9134","authenticated-orcid":false,"given":"Qinghua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0373-5734","authenticated-orcid":false,"given":"Yong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/j.cmet.2016.04.001","article-title":"Mapping the human platelet lipidome reveals cytosolic phospholipase A2 as a regulator of mitochondrial bioenergetics during activation","volume":"23","author":"Slatter","year":"2016","journal-title":"Cell Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"965954","DOI":"10.3389\/fimmu.2022.965954","article-title":"Resveratrol ameliorates oxidative stress, inflammatory response and lipid metabolism in common carp (Cyprinus carpio) fed with high-fat diet","volume":"13","author":"Wu","year":"2022","journal-title":"Front. 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