{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T10:10:54Z","timestamp":1777975854754,"version":"3.51.4"},"reference-count":77,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian Agricultural Research Corporation (Embrapa)","award":["13.16.04.005.00.00"],"award-info":[{"award-number":["13.16.04.005.00.00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Ca and P homeostasis across the egg-laying cycle is a complex process involving absorption in the small intestine, reabsorption\/excretion in the kidneys, and eggshell gland secretion. Diets with inadequate calcium and phosphorus can interfere with their absorption and digestibility, resulting in eggshell quality losses and reduced productive life, affecting egg production and welfare. A better understanding of gene expression profiles in the kidneys of laying hens during the late egg-laying period could clarify the renal role in mineral metabolism at this late stage. Therefore, the performance, egg quality and bone integrity-related traits, and expression profiles of kidney candidate genes were evaluated in 73-week-old laying hens receiving different Ca and P ratios in their diet: a high Ca\/P ratio (HR, 22.43), a low ratio (LR, 6.71), and a medium ratio (MR, 11.43). The laying hens receiving the HR diet had improved egg production and eggshell quality traits compared to the other two groups. Humerus length was shorter in the HR than in the other groups. The CYP24A1 and TRPC3 genes were differentially expressed (p.adj \u2264 0.05) among the groups. Therefore, their expression profiles could be involved in calcium and phosphate transcellular transport in 73-week-old laying hens as a way to keep mineral absorption at adequate levels.<\/jats:p>","DOI":"10.3390\/ani14101407","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T09:58:56Z","timestamp":1715162336000},"page":"1407","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens"],"prefix":"10.3390","volume":"14","author":[{"given":"Let\u00edcia Alves","family":"Salm\u00f3ria","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Universidade Estadual do Centro-Oeste, Guarapuava 85015-430, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0626-8879","authenticated-orcid":false,"given":"Adriana M\u00e9rcia Guaratini","family":"Ibelli","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Universidade Estadual do Centro-Oeste, Guarapuava 85015-430, PR, Brazil"},{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3161-019X","authenticated-orcid":false,"given":"Fernando Castro","family":"Tavernari","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Zootecnia, Centro de Educa\u00e7\u00e3o Superior do Oeste (CEO), Universidade do Estado de Santa Catarina, UDESC, Chapec\u00f3 89815-630, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Oliveira","family":"Peixoto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Universidade Estadual do Centro-Oeste, Guarapuava 85015-430, PR, Brazil"},{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Ant\u00f4nio Zanella","family":"Mor\u00e9s","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora Ester Petry","family":"Marcelino","sequence":"additional","affiliation":[{"name":"Faculdade de Conc\u00f3rdia, Conc\u00f3rdia 89700-00, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine Daenquele Silva","family":"Pinto","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, S\u00e3o Jo\u00e3o del Rei 36301-360, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1979-6021","authenticated-orcid":false,"given":"Arlei","family":"Coldebella","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Surek","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicky Lilge","family":"Kawski","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2645-4735","authenticated-orcid":false,"given":"M\u00f4nica Corr\u00eaa","family":"Ledur","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves, Conc\u00f3rdia 89715-899, SC, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Zootecnia, Centro de Educa\u00e7\u00e3o Superior do Oeste (CEO), Universidade do Estado de Santa Catarina, UDESC, Chapec\u00f3 89815-630, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"ref_1","first-page":"1155","article-title":"Effects of dietary humic substances on egg production and egg shell quality of hens after peak laying period","volume":"8","author":"Ergin","year":"2009","journal-title":"Afr. 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