{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:23:30Z","timestamp":1785147810971,"version":"3.55.0"},"reference-count":16,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T00:00:00Z","timestamp":1715212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372874"],"award-info":[{"award-number":["32372874"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>We identified Wdr17 as a highly expressed gene in pachytene spermatocytes by transcriptomic analysis of mouse testis. Germ cell-deficient infertile mouse models had significantly reduced Wdr17 expression. We performed gene interference and overexpression in the mouse spermatocyte cell line GC-2spd(ts) and investigated how Wdr17 affects spermatocyte growth and development. Our results showed that Wdr17 suppression significantly decreased cell growth rate and increased cell apoptosis in GC-2spd(ts) cells. Wdr17 suppression also arrested the cell cycle at the G1 phase. On the contrary, Wdr17 overexpression significantly promoted cell proliferation and inhibited cell apoptosis in GC-2spd(ts) cells. More cells were enriched at the S stage with a concomitant reduction of cells at the G1 stage. Wdr17 promotes mouse spermatocyte proliferation by advancing cell cycle progression and inhibiting cell apoptosis, indicating its potential role in regulating spermatogenesis in the mouse.<\/jats:p>","DOI":"10.3390\/ani14101418","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T10:31:16Z","timestamp":1715250676000},"page":"1418","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Wdr17 Regulates Cell Proliferation, Cell Cycle Progression and Apoptosis in Mouse Spermatocyte Cell Line"],"prefix":"10.3390","volume":"14","author":[{"given":"Xin","family":"Zhao","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taili","family":"Jin","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Ji","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfang","family":"Wu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicong","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4287-0026","authenticated-orcid":false,"given":"Huaqiang","family":"Yang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"R988","DOI":"10.1016\/j.cub.2017.07.067","article-title":"Spermatogenesis","volume":"27","author":"Nishimura","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1098\/rstb.2009.0323","article-title":"Evolution and spermatogenesis","volume":"365","author":"Bausek","year":"2010","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Holstein, A.F., Schulze, W., and Davidoff, M. (2003). Understanding spermatogenesis is a prerequisite for treatment. Reprod. Biol. Endocrinol., 1.","DOI":"10.1186\/1477-7827-1-107"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e992654","DOI":"10.4161\/21565562.2014.992654","article-title":"Testicular histology and germ cell cytology during spermatogenesis in the Mississippi map turtle, Graptemys pseudogeographica kohnii, from Northeast Arkansas","volume":"4","author":"Lancaster","year":"2014","journal-title":"Spermatogenesis"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, X., Zhao, X., Li, G., Zhang, M., Xing, P., Li, Z., Chen, B., Yang, H., and Wu, Z. (2021). Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation. Biol. Open, 10.","DOI":"10.1242\/bio.056804"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1038\/nature03894","article-title":"ERM is required for transcriptional control of the spermatogonial stem cell niche","volume":"436","author":"Chen","year":"2005","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"180192","DOI":"10.1038\/sdata.2018.192","article-title":"Single-cell RNA sequencing of adult mouse testes","volume":"5","author":"Lukassen","year":"2018","journal-title":"Sci. Data"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6521","DOI":"10.1038\/s41598-018-24725-0","article-title":"Characterization of germ cell differentiation in the male mouse through single-cell RNA sequencing","volume":"8","author":"Lukassen","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Li, G., Yang, X., Luo, X., Wu, Z., and Yang, H. (2023). Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair. Cell Biosci., 13.","DOI":"10.1186\/s13578-023-01159-4"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Green, C.D., Ma, Q., Manske, G.L., Shami, A.N., Zheng, X., Marini, S., Moritz, L., Sultan, C., Gurczynski, S.J., and Moore, B.B. (2018). A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq. Dev. Cell, 46.","DOI":"10.1016\/j.devcel.2018.07.025"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/s41586-022-05547-7","article-title":"The molecular evolution of spermatogenesis across mammals","volume":"613","author":"Murat","year":"2023","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Xia, B., Yan, Y., Baron, M., Wagner, F., Barkley, D., Chiodin, M., Kim, S.Y., Keefe, D.L., Alukal, J.P., and Boeke, J.D. (2020). Widespread Transcriptional Scanning in the Testis Modulates Gene Evolution Rates. Cell, 180.","DOI":"10.1016\/j.cell.2019.12.015"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Peng, Y., and Qiao, H. (2021). The Application of Single-Cell RNA Sequencing in Mammalian Meiosis Studies. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.673642"},{"key":"ref_14","first-page":"1730","article-title":"Gene expression in the mouse eye: An online resource for genetics using 103 strains of mice","volume":"15","author":"Geisert","year":"2009","journal-title":"Mol. Vis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S0167-4781(02)00499-2","article-title":"Cloning and characterization of WDR17, a novel WD repeat-containing gene on chromosome 4q34","volume":"1579","author":"Mohr","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.csbj.2019.12.009","article-title":"Novel eye genes systematically discovered through an integrated analysis of mouse transcriptomes and phenome","volume":"18","author":"Chiang","year":"2020","journal-title":"Comput. Struct. Biotechnol. J."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/10\/1418\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:42:56Z","timestamp":1760107376000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/10\/1418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,9]]},"references-count":16,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["ani14101418"],"URL":"https:\/\/doi.org\/10.3390\/ani14101418","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,9]]}}}