{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:30:58Z","timestamp":1791304258401,"version":"4.3.1"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wuhan Knowledge Innovation Special Basic Research Project","award":["2022020801010392"],"award-info":[{"award-number":["2022020801010392"]}]},{"name":"Wuhan Knowledge Innovation Special Basic Research Project","award":["2022YFD1300404"],"award-info":[{"award-number":["2022YFD1300404"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2022020801010392"],"award-info":[{"award-number":["2022020801010392"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2022YFD1300404"],"award-info":[{"award-number":["2022YFD1300404"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Porcine epidemic diarrhea virus (PEDV) has developed as a global problem for the pig business, resulting in significant financial losses. Black soldier fly extract (BFE) has been proven to improve intestinal growth in pigs after weaning. Consequently, the goal of the present investigation was to explore the effects of BFE supplementation on intestinal function in PEDV-infected piglets. Eighteen piglets were randomly allocated to three groups: control, PEDV, and BFE + PEDV. The piglets in the BFE + PEDV group received 500 mg\/kg BW of BFE orally for seven days from day 4 to 10 of the study. On day 9 of the study, six pigs from each group received either clean saline or PEDV solution at a dosage of 106 TCID50 (50% tissue culture infectious dose) per pig. On day 11, samples of blood and intestine were taken for additional investigation. The results indicated a significant decrease in the average daily gain (ADG) of piglets infected with PEDV (p &lt; 0.05). Additionally, PEDV infection led to an alteration of blood indexes and a reduction in plasma D-xylose concentration and villi height in the small intestine, while it increased plasma diamine oxidase activity and small intestinal crypt depth in piglets (p &lt; 0.05). The PEDV infection significantly reduced antioxidant enzyme activity in plasma and the gut, including total superoxide dismutase and catalase, while increasing contents of oxidation-relevant products such as malondialdehyde and hydrogen peroxide in piglets. Moreover, PEDV infection increased the mRNA expression level of antiviral-related genes (p &lt; 0.05). Nutritional supplementation with BFE improved intestinal histomorphological indicators and reduced oxidative stress produced by PEDV infection in piglets. Interestingly, BFE could significantly promote the mRNA expression level of antiviral-related genes in the ileum (p &lt; 0.05). Overall, the preliminary results suggest that dietary BFE could improve intestinal function in piglets after PEDV infection. Currently, the findings put a spotlight on the role of BFE in the prevention and treatment of PED in piglets.<\/jats:p>","DOI":"10.3390\/ani14101512","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T11:06:41Z","timestamp":1716203201000},"page":"1512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of Supplementation with Black Soldier Fly Extract on Intestinal Function in Piglets Infected with Porcine Epidemic Diarrhea Virus"],"prefix":"10.3390","volume":"14","author":[{"given":"Chenmin","family":"Yu","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanyuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-1422","authenticated-orcid":false,"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Yi","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2785-9555","authenticated-orcid":false,"given":"Yongqing","family":"Hou","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1136\/vr.100.12.243","article-title":"An apparently new syndrome of porcine epidemic diarrhoea","volume":"100","author":"Wood","year":"1977","journal-title":"Vet. Rec."},{"key":"ref_2","first-page":"219","article-title":"Experimental infection of pigs with a new porcine enteric coronavirus, CV 777","volume":"41","author":"Debouck","year":"1980","journal-title":"Am. J. Vet. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.3201\/eid1507.081256","article-title":"Chinese-like strain of porcine epidemic diarrhea virus, Thailand","volume":"15","author":"Puranaveja","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.tvjl.2015.02.017","article-title":"Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis","volume":"204","author":"Jung","year":"2015","journal-title":"Vet. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.virusres.2016.05.026","article-title":"Porcine epidemic diarrhea in China","volume":"226","author":"Wang","year":"2016","journal-title":"Virus Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"139842","DOI":"10.1016\/j.scitotenv.2020.139842","article-title":"Manure pretreatments with black soldier fly Hermetia illucens L. (Diptera: Stratiomyidae): A study to reduce pathogen content","volume":"737","author":"Awasthi","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"109156","DOI":"10.1016\/j.fsi.2023.109156","article-title":"Black soldier fly pulp in the diet of golden pompano: Effect on growth performance, liver antioxidant and intestinal health","volume":"142","author":"Li","year":"2023","journal-title":"Fish. Shellfish. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.ijbiomac.2016.07.038","article-title":"The first report of the physicochemical structure of chitin isolated from Hermetia illucens","volume":"92","author":"Bulak","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Yang, C.Y., Li, C., Xu, Z., Peng, P., Xue, C., Tomberlin, J.K., Hu, W., and Cao, Y. (2021). Black soldier fly (Hermetia illucens L.) larval diet improves CD8+ lymphocytes proliferation to eliminate chicken coronavirus at an early infection stage. Vet. Microbiol., 260.","DOI":"10.1016\/j.vetmic.2021.109151"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7371","DOI":"10.1038\/s41598-021-86765-3","article-title":"Providing live black soldier fly larvae (Hermetia illucens) improves welfare while maintaining performance of piglets post-weaning","volume":"11","author":"Ipema","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Yu, M., Li, Z., Chen, W., Rong, T., Wang, G., and Ma, X. (2019). Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs. J. Anim. Sci. Biotechnol., 10.","DOI":"10.1186\/s40104-019-0358-1"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"746224","DOI":"10.3389\/fvets.2021.746224","article-title":"Dietary Hermetia illucens Larvae Replacement Alleviates Diarrhea and Improves Intestinal Barrier Function in Weaned Piglets Challenged With Enterotoxigenic Escherichia coli K88","volume":"8","author":"Jin","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1007\/s00726-017-2397-2","article-title":"N-Acetylcysteine supplementation alleviates intestinal injury in piglets infected by porcine epidemic diarrhea virus","volume":"49","author":"Wang","year":"2017","journal-title":"Amino Acids"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1033022","DOI":"10.3389\/fvets.2022.1033022","article-title":"Tannic acid-chelated zinc supplementation alleviates intestinal injury in piglets challenged by porcine epidemic diarrhea virus","volume":"9","author":"Zhang","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00726-010-0473-y","article-title":"Dietary alpha-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets","volume":"39","author":"Hou","year":"2010","journal-title":"Amino Acids"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/jn\/138.1.60","article-title":"Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs","volume":"138","author":"Wang","year":"2008","journal-title":"J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.3945\/jn.116.236000","article-title":"Dietary Supplementation with \u03b1-Ketoglutarate Activates mTOR Signaling and Enhances Energy Status in Skeletal Muscle of Lipopolysaccharide-Challenged Piglets","volume":"146","author":"Wang","year":"2016","journal-title":"J. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Oskoueian, E., Abdullah, N., Idrus, Z., Ebrahimi, M., Goh, Y.M., Shakeri, M., and Oskoueian, A. (2014). Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-368"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1007\/s00726-018-2586-7","article-title":"Dietary supplementation with an amino acid blend enhances intestinal function in piglets","volume":"50","author":"Yi","year":"2018","journal-title":"Amino Acids"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1017\/S0007114514000038","article-title":"Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid","volume":"111","author":"Hou","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1017\/S0007114514000300","article-title":"Coated zinc oxide improves intestinal immunity function and regulates microbiota composition in weaned piglets","volume":"111","author":"Shen","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.vetmic.2017.11.031","article-title":"Identification of two antiviral inhibitors targeting 3C-like serine\/3C-like protease of porcine reproductive and respiratory syndrome virus and porcine epidemic diarrhea virus","volume":"213","author":"Shi","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3920\/JIFF2016.0055","article-title":"Nutritional value of the black soldier fly (Hermetia illucens L.) and its suitability as animal feed\u2014A review","volume":"3","author":"Dicke","year":"2017","journal-title":"J. Insects Food Feed."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3133","DOI":"10.1016\/j.psj.2020.01.018","article-title":"Black soldier fly larvae oil as an alternative fat source in broiler nutrition","volume":"99","author":"Kim","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3389\/fimmu.2020.00169","article-title":"Quantitative Proteomic Analysis Reveals Antiviral and Anti-inflammatory Effects of Puerarin in Piglets Infected With Porcine Epidemic Diarrhea Virus","volume":"11","author":"Wu","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2527\/jas.2016.1000","article-title":"Nursery pig growth performance and tissue accretion modulation due to porcine epidemic diarrhea virus or porcine deltacoronavirus challenge","volume":"95","author":"Curry","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1186\/s12985-015-0421-2","article-title":"Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus","volume":"12","author":"Lee","year":"2015","journal-title":"Virol J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.anifeedsci.2008.04.009","article-title":"Aparnak, d-Xylose absorption test: A tool for the assessment of the effect of anticoccidials on the intestinal absorptive capacity of broilers during experimental coccidiosis","volume":"148","author":"BMansoori","year":"2009","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1172\/JCI110881","article-title":"Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat","volume":"71","author":"Luk","year":"1983","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3055810","DOI":"10.1155\/2021\/3055810","article-title":"Protective Effect of Zinc Oxide and Its Association with Neutrophil Degranulation in Piglets Infected with Porcine Epidemic Diarrhea Virus","volume":"2021","author":"Zhang","year":"2021","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1152\/physrev.1994.74.1.139","article-title":"Cellular defenses against damage from reactive oxygen species","volume":"74","author":"Yu","year":"1994","journal-title":"Physiol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1177\/1753425912441955","article-title":"Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs","volume":"18","author":"Li","year":"2012","journal-title":"Innate Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.jinsphys.2011.03.017","article-title":"Antioxidant genes of the emerald ash borer (Agrilus planipennis): Gene characterization and expression profiles","volume":"57","author":"Rajarapu","year":"2011","journal-title":"J. Insect Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zhu, D., Huang, X., Tu, F., Wang, C., and Yang, F. (2020). Preparation, antioxidant activity evaluation, and identification of antioxidant peptide from black soldier fly (Hermetia illucens L.) larvae. J. Food Biochem., 44.","DOI":"10.1111\/jfbc.13186"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Phaengphairee, P., Boontiam, W., Wealleans, A., Hong, J., and Kim, Y.Y. (2023). Dietary supplementation with full-fat Hermetia illucens larvae and multi-probiotics, as a substitute for antibiotics, improves the growth performance, gut health, and antioxidative capacity of weaned pigs. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-022-03550-8"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Giannenas, I., Bonos, E., Anestis, V., Filioussis, G., Papanastasiou, D.K., Bartzanas, T., Papaioannou, N., Tzora, A., and Skoufos, I. (2017). Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169511"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Biasato, I., Ferrocino, I., Colombino, E., Gai, F., Schiavone, A., Cocolin, L., Vincenti, V., Capucchio, M.T., and Gasco, L. (2020). Effects of dietary Hermetia illucens meal inclusion on cecal microbiota and small intestinal mucin dynamics and infiltration with immune cells of weaned piglets. J. Anim. Sci. Biotechnol., 11.","DOI":"10.1186\/s40104-020-00466-x"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.aninu.2023.05.008","article-title":"Endogenous chitinase might lead to differences in growth performance and intestinal health of piglets fed different levels of black soldier fly larva meal","volume":"14","author":"Liu","year":"2023","journal-title":"Anim. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"15","DOI":"10.14304\/SURYA.JPR.V4N9.3","article-title":"New polymorphism of the influenza virus resistance Mx1 gene in Iberian domestic pigs","volume":"4","author":"Godino","year":"2016","journal-title":"Postdoc. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1017\/S0007114523001721","article-title":"Effects of monolaurin on intestinal barrier, blood biochemical profile, immunity and antioxidant function in porcine epidemic diarrhoea virus-infected piglets","volume":"131","author":"Wang","year":"2024","journal-title":"Br. J. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fvets.2020.00075","article-title":"Expression Pattern Analysis of Antiviral Genes and Inflammatory Cytokines in PEDV-Infected Porcine Intestinal Epithelial Cells","volume":"7","author":"Wang","year":"2020","journal-title":"Front. Vet. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/10\/1512\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:45:25Z","timestamp":1760107525000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/10\/1512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,20]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["ani14101512"],"URL":"https:\/\/doi.org\/10.3390\/ani14101512","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,20]]}}}