{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:25:14Z","timestamp":1790627114536,"version":"4.1.0"},"reference-count":25,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T00:00:00Z","timestamp":1716508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Science and Technology Agricultural Development Project","award":["2021-02-08-00-12-F00772"],"award-info":[{"award-number":["2021-02-08-00-12-F00772"]}]},{"name":"2018 Shanghai Outstanding Agricultural Academic Leaders Plan","award":["2021-02-08-00-12-F00772"],"award-info":[{"award-number":["2021-02-08-00-12-F00772"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Urban stray cats are cats without owners that survive in the wild for extended periods of time. They are one of the most common stray animals in cities, and as such, monitoring the pathogens carried by urban stray cats is an important component of urban epidemiological surveillance. In order to understand the prevalence of respiratory diseases in urban stray cats in Shanghai and provide scientific evidence for the development of targeted prevention and control strategies for respiratory diseases in stray cats, we collected 374 ocular, nasal, and oropharyngeal swabs from urban stray cats in Shanghai from January 2022 to December 2022. After RNA extraction, we used real-time PCR to detect six respiratory pathogens, including influenza A virus, feline calicivirus, feline herpesvirus type 1, Mycoplasma, Chlamydia, and Bordetella bronchiseptica. The results showed that among the 374 samples, 146 tested positive, with a positivity rate of 39.04%. The highest positivity rate was observed for Mycoplasma felis at 18.72% (70\/374), followed by Chlamydia felis at 11.76% (44\/374), feline calicivirus at 3.74% (14\/374), feline herpesvirus 1 at 3.48% (13\/374), Bordetella bronchiseptica at 1.34% (5\/374), and influenza A virus was not detected. The highest positivity rate for Mycoplasma felis was in Minhang District at 31.94% (23\/72), while Chlamydia felis and Bordetella bronchiseptica had the highest positivity rates in Jiading District at 23.53% (8\/34) and 5.88% (2\/34), respectively. The highest positivity rates for feline calicivirus and feline herpesvirus 1 were both observed in Qingpu District, at 14.46% (12\/83) and 9.64% (8\/83), respectively. A total of 36 samples showed mixed infections with two or more pathogens, with Mycoplasma felis being involved in 32 of these mixed infections, with the highest number of mixed infections being with Chlamydia felis at 25 samples. Respiratory pathogen positivity was detected throughout the year, with peak detection rates in summer and winter. The positivity rates of cat respiratory pathogens in different seasons showed statistical differences (\u03c72 = 27.73, p &lt; 0.01). There was no statistical difference in the positivity rates of respiratory pathogens between cats of different genders (\u03c72 = 0.92, p &gt; 0.05). The positivity rates of respiratory pathogens in cats of different age groups showed statistical differences (\u03c72 = 44.41, p &lt; 0.01). Mycoplasma felis and Chlamydia felis were the main pathogens causing respiratory infections in stray cats, with Mycoplasma felis showing a much higher positivity rate than other respiratory pathogens and often co-infecting with Chlamydia felis and feline calicivirus. The positivity rate of Mycoplasma felis was high in summer, autumn, and winter, with no statistical difference between seasons. These results indicate a serious overall prevalence of respiratory pathogens in urban stray cats in the Shanghai area, showing seasonal trends and mixed infections with other pathogens. These findings suggest the need for comprehensive prevention and control measures to address respiratory pathogen infections in urban stray cats in the Shanghai area.<\/jats:p>","DOI":"10.3390\/ani14111562","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T05:14:02Z","timestamp":1716786842000},"page":"1562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Epidemiological Surveillance of Respiratory Diseases in Urban Stray Cats in Shanghai"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8710-5119","authenticated-orcid":false,"given":"Dequan","family":"Yang","sequence":"first","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houbin","family":"Ju","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixiao","family":"Shen","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Ge","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianchao","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixin","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Animal Disease Control Center, Shanghai 201103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.cvsm.2013.10.005","article-title":"Pediatric feline upper respiratory disease","volume":"44","author":"Sykes","year":"2014","journal-title":"Vet. Clin. Small Anim."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Palombieri, A., DiProfio, F., Fruci, P., Sarchese, V., Martella, V., Marsilio, F., and Di Martino, B. (2022). Emerging Respiratory Viruses of Cats. Viruses, 14.","DOI":"10.3390\/v14040663"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hofmann-Lehmann, R., Hosie, M.J., Hartmann, K., Egberink, H., Truyen, U., Tasker, S., Bel\u00e1k, S., Boucraut-Baralon, C., Frymus, T., and Lloret, A. (2022). Calicivirus Infection in Cats. Viruses, 14.","DOI":"10.3390\/v14050937"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.rvsc.2019.02.008","article-title":"Feline calicivirus infection in cats with virulent systemic disease, Italy","volume":"124","author":"Caringella","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1002\/jmv.24216","article-title":"Detection prevalence of H5N1 avian influenza virus among stray cats in eastern China","volume":"87","author":"Zhao","year":"2015","journal-title":"J. Med. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1177\/1098612X14539087","article-title":"Feline respiratory disease: What is the role of Mycoplasma species?","volume":"16","year":"2014","journal-title":"J. Feline Med. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sand\u00f8e, P., Jensen, J.B., Jensen, F., and Nielsen, S.S. (2019). Shelters reflect but cannot solve underlying problems with relinquished and stray animals\u2014A retrospective study of dogs and cats entering and leaving shelters in Denmark from 2004 to 2017. Animals, 9.","DOI":"10.3390\/ani9100765"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Xu, J., and Jiang, A. (2023). Public opinions on stray cats in China, evidence from social media data. Animals, 13.","DOI":"10.3390\/ani13030457"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"319","DOI":"10.54203\/scil.2021.wvj44","article-title":"Stray animal population control: Methods, public health concern, ethics, and animal welfare issues","volume":"11","author":"Abdulkarim","year":"2021","journal-title":"World\u2019s Vet. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3931","DOI":"10.1007\/s00436-015-4688-4","article-title":"Urban stray cats infested by ectoparasites with zoonotic potential in Greece","volume":"114","author":"Lefkaditis","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Khademvatan, S., Abdizadeh, R., Rahim, F., Hashemitabar, M., Ghasemi, M., and Tavalla, M. (2014). Stray cats gastrointestinal parasites and its association with public health in Ahvaz City, South Western of Iran. Jundishapur J. Microbiol., 7.","DOI":"10.5812\/jjm.11079"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1136\/vr.156.21.669","article-title":"Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats: Experience from 218 European catteries","volume":"156","author":"Helps","year":"2005","journal-title":"Vet. Rec."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1177\/1040638711407479","article-title":"Development and evaluation of a real-time polymerase chain reaction method for the detection of Mycoplasma felis","volume":"23","author":"Holst","year":"2011","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.prevetmed.2015.02.012","article-title":"Nuisances and welfare of free-roaming cats in urban settings and their association with cat reproduction","volume":"119","author":"Gunther","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2055116916634109","DOI":"10.1177\/2055116916634109","article-title":"Prevalence of selected infectious disease agents in stray cats in Catalonia, Spain","volume":"2","author":"Ravicini","year":"2016","journal-title":"J. Feline Med. Surg. Open Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.tvjl.2014.05.015","article-title":"Prevalence of upper respiratory pathogens in four management models for unowned cats in the Southeast United States","volume":"201","author":"McManus","year":"2014","journal-title":"Vet. J."},{"key":"ref_17","first-page":"181","article-title":"Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydia felis, and Bordetella bronchiseptica in a population of shelter cats on Prince Edward Island","volume":"84","author":"Walter","year":"2020","journal-title":"Can. J. Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.jfms.2009.05.008","article-title":"Chlamydophila felis infection: ABCD guidelines on prevention and management","volume":"11","author":"Addie","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1177\/1098612X18813248","article-title":"Feline upper respiratory tract infection and disease in Australia","volume":"21","author":"Nguyen","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gao, J., Li, Y., Xie, Q., Al-Zaban, M.I., Al-Saeed, F.A., Shati, A.A., Al-Doaiss, A.A., Ahmed, A.E., Nawaz, S., and Ebrahem, H. (2023). Epidemiological investigation of feline upper respiratory tract infection encourages a geographically specific FCV vaccine. Vet. Sci., 10.","DOI":"10.3390\/vetsci10010046"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2055116915621581","DOI":"10.1177\/2055116915621581","article-title":"Nosocomial feline calicivirus-associated virulent systemic disease in a veterinary emergency and critical care unit in France","volume":"1","author":"Deschamps","year":"2015","journal-title":"J. Feline Med. Surg. Open Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1016\/j.ajic.2015.06.021","article-title":"Efficacy of common disinfectant\/cleaning agents in inactivating murine norovirus and feline calicivirus as surrogate viruses for human norovirus","volume":"43","author":"Chiu","year":"2015","journal-title":"Am. J. Infect. Control"},{"key":"ref_23","unstructured":"Donnett, U.B. (2014). Feline Upper Respiratory Disease Complex: The Detection and Epidemiology of Respiratory Pathogens in Midwestern Feline Shelter Populations. [Master\u2019s Thesis, Iowa State University]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1177\/1040638716646806","article-title":"Cilia-associated bacteria in fatal Bordetella bronchiseptica pneumonia of dogs and cats","volume":"28","author":"Bassel","year":"2016","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"222","DOI":"10.26444\/aaem\/100655","article-title":"Impact of way of life and environment on the prevalence of Chlamydia felis in cats as potentional sources of infection for humans","volume":"26","year":"2019","journal-title":"Ann. Agric. Environ. Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/11\/1562\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:48:29Z","timestamp":1760107709000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/11\/1562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,24]]},"references-count":25,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ani14111562"],"URL":"https:\/\/doi.org\/10.3390\/ani14111562","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,24]]}}}