{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T10:26:25Z","timestamp":1781259985054,"version":"3.54.1"},"reference-count":100,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:00:00Z","timestamp":1717718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Support Project of the National Natural Science Joint Fund","award":["U23A20224"],"award-info":[{"award-number":["U23A20224"]}]},{"name":"Key Support Project of the National Natural Science Joint Fund","award":["BR221208"],"award-info":[{"award-number":["BR221208"]}]},{"name":"Key Support Project of the National Natural Science Joint Fund","award":["QT202215"],"award-info":[{"award-number":["QT202215"]}]},{"name":"Key Support Project of the National Natural Science Joint Fund","award":["CG202445"],"award-info":[{"award-number":["CG202445"]}]},{"name":"Operating expenses of basic scientific research project of IMAU","award":["U23A20224"],"award-info":[{"award-number":["U23A20224"]}]},{"name":"Operating expenses of basic scientific research project of IMAU","award":["BR221208"],"award-info":[{"award-number":["BR221208"]}]},{"name":"Operating expenses of basic scientific research project of IMAU","award":["QT202215"],"award-info":[{"award-number":["QT202215"]}]},{"name":"Operating expenses of basic scientific research project of IMAU","award":["CG202445"],"award-info":[{"award-number":["CG202445"]}]},{"name":"High level Achievement Cultivation Project of the College of Animal Science, Inner Mongolia Agricultural University","award":["U23A20224"],"award-info":[{"award-number":["U23A20224"]}]},{"name":"High level Achievement Cultivation Project of the College of Animal Science, Inner Mongolia Agricultural University","award":["BR221208"],"award-info":[{"award-number":["BR221208"]}]},{"name":"High level Achievement Cultivation Project of the College of Animal Science, Inner Mongolia Agricultural University","award":["QT202215"],"award-info":[{"award-number":["QT202215"]}]},{"name":"High level Achievement Cultivation Project of the College of Animal Science, Inner Mongolia Agricultural University","award":["CG202445"],"award-info":[{"award-number":["CG202445"]}]},{"name":"Special Project for Cultivating High-level Achievements of the College of Animal Science, Inner Mongolia Agricultural University","award":["U23A20224"],"award-info":[{"award-number":["U23A20224"]}]},{"name":"Special Project for Cultivating High-level Achievements of the College of Animal Science, Inner Mongolia Agricultural University","award":["BR221208"],"award-info":[{"award-number":["BR221208"]}]},{"name":"Special Project for Cultivating High-level Achievements of the College of Animal Science, Inner Mongolia Agricultural University","award":["QT202215"],"award-info":[{"award-number":["QT202215"]}]},{"name":"Special Project for Cultivating High-level Achievements of the College of Animal Science, Inner Mongolia Agricultural University","award":["CG202445"],"award-info":[{"award-number":["CG202445"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Testicular development and spermatogenesis are tightly regulated by both coding and non-coding genes, with mRNA and lncRNA playing crucial roles in post-transcriptional gene expression regulation. However, there are significant differences in regulatory mechanisms before and after sexual maturity. Nevertheless, the mRNAs and lncRNAs in the testes of Mongolian horses have not been systematically identified. In this study, we first identified the testicular tissues of sexually immature and sexually mature Mongolian horses at the tissue and protein levels, and comprehensively analyzed the expression profiles of mRNA and lncRNA in the testes of 1-year-old (12 months, n = 3) and 10-year-old (n = 3) Mongolian horses using RNA sequencing technology. Through gene expression analysis, we identified 16,582 mRNAs and 2128 unknown lncRNAs that are commonly expressed in both sexually immature and sexually mature Mongolian horses. Meanwhile, 9217 mRNAs (p &lt; 0.05) and 2191 unknown lncRNAs (p &lt; 0.05) were identified as differentially expressed between the two stages, which were further validated by real-time fluorescent quantitative PCR and analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The analysis results showed that genes in the sexually immature stage were mainly enriched in terms related to cellular infrastructure, while genes in the sexually mature stage were enriched in terms associated with hormones, metabolism, and spermatogenesis. In summary, the findings of this study provide valuable resources for a deeper understanding of the molecular mechanisms underlying testicular development and spermatogenesis in Mongolian horses and offer new perspectives for future related research.<\/jats:p>","DOI":"10.3390\/ani14121717","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T10:43:42Z","timestamp":1717757022000},"page":"1717","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1999-4120","authenticated-orcid":false,"given":"Yuanyi","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"},{"name":"Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China"},{"name":"Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3120-6445","authenticated-orcid":false,"given":"Ming","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"},{"name":"Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China"},{"name":"Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8457-9364","authenticated-orcid":false,"given":"Qianqian","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"},{"name":"Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China"},{"name":"Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7084-1749","authenticated-orcid":false,"given":"Jialong","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"},{"name":"Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China"},{"name":"Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1125-3030","authenticated-orcid":false,"given":"Bilig","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"},{"name":"Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China"},{"name":"Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, 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Cell Dev. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18714","DOI":"10.1074\/jbc.RA119.010496","article-title":"Distinct H3K9me3 and DNA methylation modifications during mouse spermatogenesis","volume":"294","author":"Liu","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Jin, K., Zhou, J., Zuo, Q., Song, J., Zhang, Y., Chang, G., Chen, G., and Li, B. (2020). Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus). Animals, 10.","DOI":"10.3390\/ani10122228"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1152\/physrev.00009.2020","article-title":"Sperm bauplan and function and underlying processes of sperm formation and selection","volume":"102","author":"Teves","year":"2022","journal-title":"Physiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.tibs.2023.03.007","article-title":"LncRNAs: The missing link to senescence nuclear architecture","volume":"48","author":"Han","year":"2023","journal-title":"Trends Biochem. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.7150\/ijbs.40769","article-title":"LncRNAs regulate metabolism in cancer","volume":"16","author":"Lin","year":"2020","journal-title":"Int. J. Biol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1161\/CIRCRESAHA.118.313447","article-title":"LncRNAs Coming of Age","volume":"123","author":"Grote","year":"2018","journal-title":"Circ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.7150\/ijbs.16968","article-title":"LncRNAs: From Basic Research to Medical Application","volume":"13","author":"Wu","year":"2017","journal-title":"Int. J. Biol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bridges, M.C., Daulagala, A.C., and Kourtidis, A. (2021). LNCcation: lncRNA localization and function. J. Cell Biol., 220.","DOI":"10.1083\/jcb.202009045"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.cell.2018.01.011","article-title":"Functional Classification and Experimental Dissection of Long Noncoding RNAs","volume":"172","author":"Kopp","year":"2018","journal-title":"Cell"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Hong, S.H., Kwon, J.T., Kim, J., Jeong, J., Kim, J., Lee, S., and Cho, C. (2018). Profiling of testis-specific long noncoding RNAs in mice. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4931-3"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.ygeno.2017.07.001","article-title":"Genome-wide analysis of long non-coding RNAs and their role in postnatal porcine testis development","volume":"109","author":"Weng","year":"2017","journal-title":"Genomics"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ran, M., Chen, B., Li, Z., Wu, M., Liu, X., He, C., Zhang, S., and Li, Z. (2016). Systematic Identification of Long Noncoding RNAs in Immature and Mature Porcine Testes. Biol. Reprod., 94.","DOI":"10.1095\/biolreprod.115.136911"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zhao, S., Chen, T., Luo, X., Chen, S., Wang, J., Lai, S., and Jia, X. (2021). Identification of Novel lncRNA and Differentially Expressed Genes (DEGs) of Testicular Tissues among Cattle, Yak, and Cattle-Yak Associated with Male Infertility. Animals, 11.","DOI":"10.3390\/ani11082420"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1136\/vr.135.2.31","article-title":"Is dentition an accurate indication of the age of a horse?","volume":"135","author":"Richardson","year":"1994","journal-title":"Vet. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1177\/089875640802500304","article-title":"Morphology of the occlusal surfaces of premolar and molar teeth as an indicator of age in the horse","volume":"25","author":"Carmalt","year":"2008","journal-title":"J. Vet. Dent."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Xing, J., Qi, X., Liu, G., Li, X., Gao, X., Bou, G., Bai, D., Zhao, Y., Du, M., and Dugarjaviin, M. (2023). A Transcriptomic Regulatory Network among miRNAs, lncRNAs, circRNAs, and mRNAs Associated with L-leucine-induced Proliferation of Equine Satellite Cells. Animals, 13.","DOI":"10.3390\/ani13020208"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1080\/14789450.2017.1388167","article-title":"Protein purification and analysis: Next generation Western blotting techniques","volume":"14","author":"Mishra","year":"2017","journal-title":"Expert. Rev. Proteom."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1071\/RD19133","article-title":"Analysis of the miRNA transcriptome during testicular development and spermatogenesis of the Mongolian horse","volume":"32","author":"Li","year":"2020","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bao, T., Han, H., Li, B., Zhao, Y., Bou, G., Zhang, X., Du, M., Zhao, R., Mongke, T. (2020). The distinct transcriptomes of fast-twitch and slow-twitch muscles in Mongolian horses. Comp. Biochem. Physiol. Part. D Genom. Proteom., 33.","DOI":"10.1016\/j.cbd.2019.100649"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"103458","DOI":"10.1016\/j.jevs.2021.103458","article-title":"A Fast PCR Test for the Simultaneous Identification of Species and Gender in Horses, Donkeys, Mules and Hinnies","volume":"102","author":"Wang","year":"2021","journal-title":"J. Equine Vet. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Han, H., McGivney, B.A., Allen, L., Bai, D., Corduff, L.R., Davaakhuu, G., Davaasambuu, J., Dorjgotov, D., Hall, T.J., and Hemmings, A.J. (2022). Common protein-coding variants influence the racing phenotype in galloping racehorse breeds. Commun. Biol., 5.","DOI":"10.1038\/s42003-022-04206-x"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Chao, H.P., Chen, Y., Takata, Y., Tomida, M.W., Lin, K., Kirk, J.S., Simper, M.S., Mikulec, C.D., Rundhaug, J.E., and Fischer, S.M. (2019). Systematic evaluation of RNA-Seq preparation protocol performance. BMC Genom., 20.","DOI":"10.1186\/s12864-019-5953-1"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Knierim, E., Lucke, B., Schwarz, J.M., Schuelke, M., and Seelow, D. (2011). Systematic comparison of three methods for fragmentation of long-range PCR products for next generation sequencing. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028240"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1016\/j.molcel.2022.05.027","article-title":"Integrated lncRNA function upon genomic and epigenomic regulation","volume":"82","author":"Herman","year":"2022","journal-title":"Mol. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1111\/jcmm.13866","article-title":"The novel regulatory role of lncRNA-miRNA-mRNA axis in cardiovascular diseases","volume":"22","author":"Huang","year":"2018","journal-title":"J. Cell Mol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ren, X., Liu, Y., Zhao, Y., Li, B., Bai, D., Bou, G., Zhang, X., Du, M., Wang, X., and Bou, T. (2022). Analysis of the Whole-Genome Sequences from an Equus Parent-Offspring Trio Provides Insight into the Genomic Incompatibilities in the Hybrid Mule. Genes, 13.","DOI":"10.3390\/genes13122188"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1080\/02664763.2020.1788518","article-title":"A comparison study on modeling of clustered and overdispersed count data for multiple comparisons","volume":"48","author":"Kruppa","year":"2020","journal-title":"J. Appl. Stat."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.2527\/1992.7061971x","article-title":"Use and misuse of multiple comparisons in animal experiments","volume":"70","author":"Lowry","year":"1992","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.theriogenology.2016.04.052","article-title":"Managing the reproductive performance of beef cows","volume":"86","author":"Diskin","year":"2016","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10272","DOI":"10.3168\/jds.2017-13399","article-title":"A 100-Year Review: Historical development of female reproductive physiology in dairy cattle","volume":"100","author":"Thatcher","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"106905","DOI":"10.1016\/j.anireprosci.2021.106905","article-title":"MTNR1A gene polymorphisms and reproductive recovery after seasonal anoestrus in different Mediterranean sheep breeds","volume":"236","author":"Pulinas","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.cub.2020.10.003","article-title":"Male-Mediated Maturation in Wild Geladas","volume":"31","author":"Lu","year":"2021","journal-title":"Curr. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1093\/humrep\/dead054","article-title":"Cancer cell contamination and decontamination methods for ovaries and testes: Special focus on prepubertal gonads with a view to safe fertility restoration","volume":"38","author":"Kourta","year":"2023","journal-title":"Hum. Reprod."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Luo, H., Zhou, H., Jiang, S., He, C., Xu, K., Ding, J., Liu, J., Qin, C., Chen, K., and Zhou, W. (2023). Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914597"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1080\/15476278.2016.1145318","article-title":"Studies of gonadal sex differentiation","volume":"12","author":"Makiyan","year":"2016","journal-title":"Organogenesis"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1111\/febs.15848","article-title":"Immune and vascular contributions to organogenesis of the testis and ovary","volume":"289","author":"Gu","year":"2022","journal-title":"FEBS J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.ydbio.2014.02.012","article-title":"Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads","volume":"389","author":"Lambeth","year":"2014","journal-title":"Dev. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1111\/rda.14373","article-title":"Integrated testis transcriptome and whole genome analysis of sexual maturity in Large White and Tongcheng pigs","volume":"58","author":"Li","year":"2023","journal-title":"Reprod. Domest. Anim."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1439-0531.2008.01177.x","article-title":"Sexual maturation in the bull","volume":"43","author":"Rawlings","year":"2008","journal-title":"Reprod. Domest. Anim."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"662887","DOI":"10.3389\/fvets.2021.662887","article-title":"Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere","volume":"8","author":"Ebel","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1111\/rda.14203","article-title":"Multispecies comparative analysis reveals transcriptional specificity during Mongolian horse testicular development","volume":"57","author":"Zhang","year":"2022","journal-title":"Reprod. Domest. Anim."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1111\/rda.14432","article-title":"The role of GnRH in Tibetan male sheep and goat reproduction","volume":"58","author":"Zhao","year":"2023","journal-title":"Reprod. Domest. Anim."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chen, J., Chen, X., Guo, W., Tang, W., Zhang, Y., Tian, X., and Zou, Y. (2024). Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats. BMC Vet. Res., 20.","DOI":"10.1186\/s12917-024-03932-0"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1439-0531.2012.02137.x","article-title":"Expression of 11\u03b2-hydroxysteroid dehydrogenase type 1 and glucocorticoid receptors in reproductive tissue of male horses at different stages of sexual maturity","volume":"48","author":"Budik","year":"2013","journal-title":"Reprod. Domest. Anim."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/S0093-691X(98)00178-2","article-title":"Peripuberal sexual development of Pantaneiro stallions","volume":"50","author":"Melo","year":"1998","journal-title":"Theriogenology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"103422","DOI":"10.1016\/j.jevs.2021.103422","article-title":"Nonlinear Models to Describe the Testicular Size Growth Curve of Mangalarga Marchador Stallions","volume":"102","author":"Silva","year":"2021","journal-title":"J. Equine Vet. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1038\/s41598-019-41475-9","article-title":"Identification of piRNAs and piRNA clusters in the testes of the Mongolian horse","volume":"9","author":"Li","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Budsuren, U., Ulaangerel, T., Shen, Y., Liu, G., Davshilt, T., Yi, M., Bold, D., Zhang, X., Bai, D., and Dorjgotov, D. (2022). MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies. Genes, 13.","DOI":"10.3390\/genes13101836"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Liu, Y., Du, M., Li, X., Zhang, L., Zhao, B., Wang, N., and Dugarjaviin, M. (2024). Single-Cell Transcriptome Sequencing Reveals Molecular Expression Differences and Marker Genes in Testes during the Sexual Maturation of Mongolian Horses. Animals, 14.","DOI":"10.3390\/ani14091258"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Bhale, A.S., and Venkataraman, K. (2022). Leveraging knowledge of HDLs major protein ApoA1: Structure, function, mutations, and potential therapeutics. Biomed. Pharmacother., 154.","DOI":"10.1016\/j.biopha.2022.113634"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Umer, S., Zhao, S.J., Sammad, A., Weldegebriall Sahlu, B., Yunwei, P., and Zhu, H. (2019). AMH: Could It Be Used as A Biomarker for Fertility and Superovulation in Domestic Animals?. Genes, 10.","DOI":"10.3390\/genes10121009"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.theriogenology.2023.12.021","article-title":"TAC3 regulates GnRH\/gonadotropin synthesis in female chickens","volume":"215","author":"Meng","year":"2024","journal-title":"Theriogenology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e15381","DOI":"10.7717\/peerj.15381","article-title":"Association between INHA gene polymorphisms and litter size in Hainan black goats","volume":"11","author":"Bian","year":"2023","journal-title":"PeerJ"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1096\/fasebj.8.2.8119487","article-title":"The biology of SPARC, a protein that modulates cell-matrix interactions","volume":"8","author":"Lane","year":"1994","journal-title":"FASEB J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1038\/s41556-023-01184-y","article-title":"The pioneer factor SOX9 competes for epigenetic factors to switch stem cell fates","volume":"25","author":"Yang","year":"2023","journal-title":"Nat. Cell Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"dev200330","DOI":"10.1242\/dev.200330","article-title":"Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice","volume":"149","author":"Merges","year":"2022","journal-title":"Development"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"jcs258426","DOI":"10.1242\/jcs.258426","article-title":"Dissecting the PRSS37 interactome and potential mechanisms leading to ADAM3 loss in PRSS37-null sperm","volume":"134","author":"Xiong","year":"2021","journal-title":"J. Cell Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1095\/biolreprod.108.070243","article-title":"HMGB4, a novel member of the HMGB family, is preferentially expressed in the mouse testis and localizes to the basal pole of elongating spermatids","volume":"80","author":"Catena","year":"2009","journal-title":"Biol. Reprod."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"21672","DOI":"10.1073\/pnas.0912432106","article-title":"Prepubertal human spermatogonia and mouse gonocytes share conserved gene expression of germline stem cell regulatory molecules","volume":"106","author":"Wu","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.anireprosci.2006.02.009","article-title":"Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey","volume":"97","author":"Bakst","year":"2007","journal-title":"Anim. Reprod. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/s41422-018-0099-2","article-title":"The adult human testis transcriptional cell atlas","volume":"28","author":"Guo","year":"2018","journal-title":"Cell Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1101\/gad.349550.122","article-title":"Histone methyltransferase DOT1L is essential for self-renewal of germline stem cells","volume":"36","author":"Lin","year":"2022","journal-title":"Genes. Dev."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1016\/j.celrep.2019.01.045","article-title":"The Neonatal and Adult Human Testis Defined at the Single-Cell Level","volume":"26","author":"Sohni","year":"2019","journal-title":"Cell Rep."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1210\/jc.2018-02010","article-title":"DLK1 Is a Novel Link Between Reproduction and Metabolism","volume":"104","author":"Gomes","year":"2019","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1053\/j.gastro.2023.03.228","article-title":"Macrophage-derived Osteopontin (SPP1) Protects from Nonalcoholic Steatohepatitis","volume":"165","author":"Han","year":"2023","journal-title":"Gastroenterology"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Olivieri, A., Lee, R.S., Fratini, F., Keutcha, C., Chaand, M., Mangano, V., Celani, F., Mochi, S., Birago, C., and Paone, S. (2021). Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility. Commun. Biol., 4.","DOI":"10.1038\/s42003-021-02900-w"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"11800","DOI":"10.1038\/s41598-019-48089-1","article-title":"Transcriptomic Profiling of Adipose Derived Stem Cells Undergoing Osteogenesis by RNA-Seq","volume":"9","author":"Shaik","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"eabg7265","DOI":"10.1126\/sciadv.abg7265","article-title":"Desmosomal proteins of DSC2 and PKP1 promote cancer cells survival and metastasis by increasing cluster formation in circulatory system","volume":"7","author":"Li","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e22680","DOI":"10.1096\/fj.202101667RR","article-title":"Involvement of GLCCI1 in mouse spermatogenesis","volume":"37","author":"Takada","year":"2023","journal-title":"FASEB J."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1172\/JCI41303","article-title":"The key role of vitamin A in spermatogenesis","volume":"120","author":"Hogarth","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.semcdb.2021.05.032","article-title":"Nonclassical androgen and estrogen signaling is essential for normal spermatogenesis","volume":"121","author":"Cooke","year":"2022","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00345-003-0365-9","article-title":"Androgens and male fertility","volume":"21","author":"Dohle","year":"2003","journal-title":"World J. Urol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2014.03.007","article-title":"Regulation of spermatogenesis: An evolutionary biologist\u2019s perspective","volume":"29","author":"Schlatt","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s00018-012-1079-1","article-title":"Hormonal control of Sertoli cell metabolism regulates spermatogenesis","volume":"70","author":"Alves","year":"2013","journal-title":"Cell Mol. Life Sci."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Lai, X., Peng, S., Liu, L., Zou, Z., Cao, L., and Wang, Y. (2023). Tissue-specific promoters regulate the transcription of cyp19a1 in the brain-pituitary-gonad axis of Japanese eel (Anguilla japonica). J. Steroid Biochem. Mol. Biol., 232.","DOI":"10.1016\/j.jsbmb.2023.106334"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1080\/03602530903112284","article-title":"Hormonal regulation of CYP1A expression","volume":"41","author":"Monostory","year":"2009","journal-title":"Drug Metab. Rev."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Pe\u00f1a, F.J. (2020). Molecular Biology of Spermatozoa. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093060"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s00441-015-2249-x","article-title":"Proteomics of post-translational modifications of mammalian spermatozoa","volume":"363","author":"Baker","year":"2016","journal-title":"Cell Tissue Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/andr.12569","article-title":"The serine protease testisin is present on the surface of capacitated stallion spermatozoa and interacts with key zona pellucida binding proteins","volume":"7","author":"Swegen","year":"2019","journal-title":"Andrology"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/evj.12308","article-title":"Stallion fertility: A focus on the spermatozoon","volume":"47","author":"Varner","year":"2015","journal-title":"Equine Vet. J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"106848","DOI":"10.1016\/j.anireprosci.2021.106848","article-title":"A stallion spermatozoon\u2019s journey through the mare\u2019s genital tract: In vivo and in vitro aspects of sperm capacitation","volume":"246","author":"Maitan","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"844","DOI":"10.4103\/1008-682X.186880","article-title":"Importance of sperm morphology during sperm transport and fertilization in mammals","volume":"18","author":"Gadea","year":"2016","journal-title":"Asian J. Androl."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.theriogenology.2014.05.026","article-title":"Sperm motility parameters and spermatozoa morphometric characterization in marine species: A study of swimmer and sessile species","volume":"82","author":"Gallego","year":"2014","journal-title":"Theriogenology"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1016\/j.fertnstert.2008.08.088","article-title":"A new real-time morphology classification for human spermatozoa: A link for fertilization and improved embryo quality","volume":"92","author":"Cassuto","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/hmg\/ddx422","article-title":"DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport","volume":"27","author":"Hartill","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1016\/j.bbrc.2018.08.104","article-title":"Development of xeno-free epithelial differentiation media for adherent, non-expanded adipose stromal vascular cell cultures","volume":"503","author":"Hintze","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1007\/s00018-024-05147-w","article-title":"Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice","volume":"81","author":"Li","year":"2024","journal-title":"Cell Mol. Life Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1177\/154405910808701214","article-title":"Methyl methacrylate activates the Gsta1 promoter","volume":"87","author":"Hattori","year":"2008","journal-title":"J. Dent. Res."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"113641","DOI":"10.1016\/j.celrep.2023.113641","article-title":"Genetic insights into carbohydrate sulfotransferase 8 and its impact on the immunotherapy efficacy of cancer","volume":"43","author":"Chou","year":"2024","journal-title":"Cell Rep."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s00335-023-10023-2","article-title":"Identification of genetic variants affecting reproduction traits in Vrindavani cattle","volume":"35","author":"Gangwar","year":"2024","journal-title":"Mamm. Genome"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1071\/RD17511","article-title":"RNA sequencing and differential expression reveals the effects of serial oestrus synchronisation on ovarian genes in dairy goats","volume":"30","author":"Sun","year":"2018","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Liang, Z., Prakapenka, D., VanRaden, P.M., Jiang, J., Ma, L., and Da, Y. (2023). A Million-Cow Genome-Wide Association Study of Three Fertility Traits in U.S. Holstein Cows. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241310496"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ygeno.2012.01.005","article-title":"Whole-exome sequencing in a single proband reveals a mutation in the CHST8 gene in autosomal recessive peeling skin syndrome","volume":"99","author":"Cabral","year":"2012","journal-title":"Genomics"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"9218","DOI":"10.1093\/nar\/gkaa659","article-title":"The nucleic acid chaperone activity of the HIV-1 Gag polyprotein is boosted by its cellular partner RPL7: A kinetic study","volume":"48","author":"Karnib","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1002\/vms3.783","article-title":"Expression profile analysis of sheep ovary after superovulation and estrus synchronisation treatment","volume":"8","author":"Wang","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1093\/nar\/19.5.1099","article-title":"Identification of a gene encoding the predicted ribosomal protein L7b divergently transcribed from POL1 in fission yeast Schizosaccharomyces pombe","volume":"19","author":"Damagnez","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1080\/14647273.2022.2144771","article-title":"Identification of potential key genes related to idiopathic male infertility using RNA-sequencing data: An in-silico approach","volume":"26","author":"Lukkani","year":"2023","journal-title":"Hum. Fertil."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1021\/sb200005w","article-title":"Feedback control of protein expression in mammalian cells by tunable synthetic translational inhibition","volume":"1","author":"Stapleton","year":"2012","journal-title":"ACS Synth. Biol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/12\/1717\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:55:22Z","timestamp":1760108122000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/12\/1717"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,7]]},"references-count":100,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ani14121717"],"URL":"https:\/\/doi.org\/10.3390\/ani14121717","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,7]]}}}