{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:52:34Z","timestamp":1772553154978,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lithuanian Council for Research","award":["S-MIP-22-137"],"award-info":[{"award-number":["S-MIP-22-137"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The hypothesis of this study was that there were changes in biomarkers registered by innovative technologies in cows with subclinical acidosis. The aim of this study was to identify changes in the in-line milk fat-to-protein ratio and cow feeding behaviors such as reticulorumen pH, reticulorumen temperature, cow activity, and water intake with subclinical acidosis. From a total of 98 cows, 59 cows were selected to meet the following criteria (2 or more lactations, with 31 days in milk (DIM)). The selected animals were separated into two groups based on general clinical examination and reticulorumen pH: the subclinical acidosis group (SCA, n = 23) and the healthy group (HC, n = 36). During the diagnosis of subclinical acidosis and following the clinical examination of the healthy group using the BROLIS HerdLine system, the daily averages of milk yield (kg\/day), milk fat (%), milk protein (%), and the milk fat-to-protein ratio were recorded. Simultaneously, by using Smaxtec technology, reticulorumen parameters and cow activity, including pH, temperature (\u00b0C), rumination time (minutes\/day), and water intake (hours\/day), were registered. Changes in parameters measured using innovative technologies were able to identify cows with subclinical acidosis. Cows with subclinical acidosis had a lower reticulorumen pH by 18.8% (p &lt; 0.0001), a decreased milk yield by 10.49% (p &lt; 0.001), a lower milk fat-to-protein ratio by 11.88% (p &lt; 0.01), and a decreased rumination time by 6.59% (p &lt; 0.01). However, the activity of these cows was higher by 57.19% (p &lt; 0.001) compared to healthy cows. From a practical point of view, we suggest that veterinarians and farmers track parameters such as reticulorumen pH, milk yield, milk fat-to-protein ratio, rumination time, and activity for the identification of subclinical acidosis.<\/jats:p>","DOI":"10.3390\/ani14131883","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T07:20:06Z","timestamp":1719386406000},"page":"1883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Changes in Parameters Registered by Innovative Technologies in Cows with Subclinical Acidosis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9993-2224","authenticated-orcid":false,"given":"Ram\u016bnas","family":"Antanaitis","sequence":"first","affiliation":[{"name":"Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Til\u017e\u0117s Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5343-6590","authenticated-orcid":false,"given":"Karina","family":"D\u017eermeikait\u0117","sequence":"additional","affiliation":[{"name":"Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Til\u017e\u0117s Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0462-1463","authenticated-orcid":false,"given":"Justina","family":"Kri\u0161tolaityt\u0117","sequence":"additional","affiliation":[{"name":"Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Til\u017e\u0117s Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolandas","family":"Stankevi\u010dius","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gintaras","family":"Daunoras","sequence":"additional","affiliation":[{"name":"L. Kriau\u010deli\u016bnas Small Animal Clinic, Veterinary Faculty, Lithuanian University of Health Sciences, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8572-2616","authenticated-orcid":false,"given":"Mindaugas","family":"Televi\u010dius","sequence":"additional","affiliation":[{"name":"Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Til\u017e\u0117s Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dovil\u0117","family":"Mala\u0161auskien\u0117","sequence":"additional","affiliation":[{"name":"Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Til\u017e\u0117s Str. 18, LT-47181 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4938-168X","authenticated-orcid":false,"given":"John","family":"Cook","sequence":"additional","affiliation":[{"name":"RCVS Recognised Specialist Cattle Health and Production, Technical Veterinarian, Avenida de los Robles Visalia, Visalia, CA 93291, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8972-1327","authenticated-orcid":false,"given":"Lorenzo","family":"Viora","sequence":"additional","affiliation":[{"name":"Scottish Centre for Production Animal Health and Food Safety, School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow G12 8QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.3168\/jds.S0022-0302(02)74420-2","article-title":"Estimation of Energy Balance at the Individual and Herd Level Using Blood and Milk Traits in High-Yielding Dairy Cows1,2","volume":"85","author":"Reist","year":"2002","journal-title":"J. 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