{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:43:21Z","timestamp":1790905401618,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key R &amp; D projects of Ningxia Hui Autonomous Region","award":["2021BEE02032"],"award-info":[{"award-number":["2021BEE02032"]}]},{"name":"Key R &amp; D projects of Ningxia Hui Autonomous Region","award":["172102110201"],"award-info":[{"award-number":["172102110201"]}]},{"name":"Key R &amp; D projects of Ningxia Hui Autonomous Region","award":["212102110005"],"award-info":[{"award-number":["212102110005"]}]},{"name":"Key R &amp; D projects of Ningxia Hui Autonomous Region","award":["NGSB-2021-12-05"],"award-info":[{"award-number":["NGSB-2021-12-05"]}]},{"name":"Science and Technology Development Plan Project of Henan province of China","award":["2021BEE02032"],"award-info":[{"award-number":["2021BEE02032"]}]},{"name":"Science and Technology Development Plan Project of Henan province of China","award":["172102110201"],"award-info":[{"award-number":["172102110201"]}]},{"name":"Science and Technology Development Plan Project of Henan province of China","award":["212102110005"],"award-info":[{"award-number":["212102110005"]}]},{"name":"Science and Technology Development Plan Project of Henan province of China","award":["NGSB-2021-12-05"],"award-info":[{"award-number":["NGSB-2021-12-05"]}]},{"name":"Science and Technology Innovation Demonstration Project for High Quality Agricultural Development and Ecological Protection of the Ningxia Academy of Agriculture and Forestry Sciences","award":["2021BEE02032"],"award-info":[{"award-number":["2021BEE02032"]}]},{"name":"Science and Technology Innovation Demonstration Project for High Quality Agricultural Development and Ecological Protection of the Ningxia Academy of Agriculture and Forestry Sciences","award":["172102110201"],"award-info":[{"award-number":["172102110201"]}]},{"name":"Science and Technology Innovation Demonstration Project for High Quality Agricultural Development and Ecological Protection of the Ningxia Academy of Agriculture and Forestry Sciences","award":["212102110005"],"award-info":[{"award-number":["212102110005"]}]},{"name":"Science and Technology Innovation Demonstration Project for High Quality Agricultural Development and Ecological Protection of the Ningxia Academy of Agriculture and Forestry Sciences","award":["NGSB-2021-12-05"],"award-info":[{"award-number":["NGSB-2021-12-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Probiotics are a prominent alternative to antibiotics in antimicrobial-free broiler farming. To assess the effect of Sphingomonas sp. Z392 (isolated and identified) on broiler growth, 600 one-day-old Kebao broiler chickens were randomly divided into a control group and an experimental group. Each group had three replicates, with 100 broiler chickens being raised in each replicate. Regarding the experimental group of broiler chickens, 4.0 \u00d7 105 CFU\/mL of Sphingomonas Z392 was added to their drinking water. Then, the changes in broiler body weight, the EPI, intestinal histological structure, and gut microbiota were examined. The results show that the supplementation of the broilers\u2019 drinking water with 4 \u00d7 105 CFU\/mL of Sphingomonas Z392 resulted in an increase in the relative abundance of Lactobacillus, Bacteroides, Lachnospiraceae, Aminobacterium, Oribacterium, Christensenellaceae, Faecalibacterium, Barnesiella, Ruminococcus, Parabacteroides, Phascolarctobacterium, Butyricicoccaceae, and Caproiciproducens, which have been reported to be positively correlated with the improved digestion and absorption of broiler chickens. The relative abundance of Odoribacter, Alistipes, Parabacteroides, and Rikenellaceae increased, and these have been reported to be negatively correlated with the occurrence of intestinal diseases. The relative abundance of Campylobacter, Shigella Castellani, Bilophila, Campylobacter, Clostridia, and Anaerotruncus decreased, and these have been reported to be positively correlated with the occurrence of intestinal diseases. At the same time, the following also increased: the integrity of small intestinal villus morphology; the number of goblet cells in small intestinal epithelial cells; the health of the mitochondria in the cytoplasm of jejunal villous epithelial cells; the number of lysosomes in the cytoplasm of goblet cells in the small intestinal epithelium, ileal villous epithelial cells, and mitochondria in the cytoplasm of large intestinal villous epithelial cells; the VH\/CD of the ileum; and digestive, absorption, and defense capabilities. In particular, the final weight increased by 4.33%, and the EPI increased by 10.10%. Therefore, the supplementation of broiler drinking water with Sphingomonas generated better economic benefits from the broiler chickens.<\/jats:p>","DOI":"10.3390\/ani14131920","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T04:53:27Z","timestamp":1719809607000},"page":"1920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of Adding Sphingomonas Z392 to Drinking Water on Growth Performance, Intestinal Histological Structure, and Microbial Community of Broiler Chickens"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7755-2845","authenticated-orcid":false,"given":"Mingcheng","family":"Wang","sequence":"first","affiliation":[{"name":"National Laboratory of Agricultural Microbiology, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Wuhan 430070, China"},{"name":"Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Biological and Food Engineering, Huanghuai University, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Huanghuai University, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-1637","authenticated-orcid":false,"given":"Yanan","family":"Guo","sequence":"additional","affiliation":[{"name":"Animal Science Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Animal Disease Prevention and Quarantine Center of Zhumadian City, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Huanghuai University, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gailing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Huanghuai University, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoying","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Huanghuai University, Zhumadian 463000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aizhen","family":"Guo","sequence":"additional","affiliation":[{"name":"National Laboratory of Agricultural Microbiology, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Wuhan 430070, China"},{"name":"Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zou, Q., Fan, X., Xu, Y., Wang, T., and Li, D. 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