{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:04:09Z","timestamp":1791374649720,"version":"4.3.1"},"reference-count":42,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Institute of Agriculture, Tokai University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The areas of the Mount Aso grasslands in Kumamoto, Japan, are the primary location for the breeding of the Kumamoto strain of Japanese Brown cattle (JBRK). Although Aso limonite, deposited by volcanic ash and magma, has been commonly fed to pregnant JBRK in this area, the mechanisms of its salutary effects on pregnant JBRK have not yet been elucidated. Approximately 100 days before the expected day of calf delivery, seven JBRK (four supplemented with limonite and three controls without limonite) were assigned to this study, from which a buccal swab was collected at the highest rumination every 30 days for 90 days. DNA extracted from these swabs was then analyzed using a 16S rRNA gene amplicon sequence analysis. Statistically significant differences between the two groups were discovered through beta-diversity analysis, though results from alpha-diversity analysis were inconclusive. The microbiota identified were classified into six clusters, and three of the main clusters were core-rumen bacteria, primarily cellulose digestion in cluster 1, oral bacteria in cluster 2, and non-core-rumen bacteria in cluster 3. In the limonite group, core-rumen bacteria decreased while non-core-rumen bacteria increased, suggesting that limonite feeding alters rumen microbiota, particularly activation of non-core-rumen microbiota.<\/jats:p>","DOI":"10.3390\/ani14131968","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T08:45:34Z","timestamp":1719996334000},"page":"1968","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Buccal Swab Samples from Japanese Brown Cattle Fed with Limonite Reveal Altered Rumen Microbiome"],"prefix":"10.3390","volume":"14","author":[{"given":"Kentaro","family":"Harakawa","sequence":"first","affiliation":[{"name":"Research Institute of Agriculture, Tokai University, Kumamoto 862-8652, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1654-960X","authenticated-orcid":false,"given":"Shinpei","family":"Kawarai","sequence":"additional","affiliation":[{"name":"Research Institute of Agriculture, Tokai University, Kumamoto 862-8652, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0286-0288","authenticated-orcid":false,"given":"Kirill","family":"Kryukov","sequence":"additional","affiliation":[{"name":"Center for Genome Informatics, Joint Support-Center for Data Science Research, Research Organization of Information and Systems, Mishima 411-8540, Shizuoka, Japan"},{"name":"Bioinformation and DDBJ Center, National Institute of Genetics, Mishima 411-8540, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1760-3839","authenticated-orcid":false,"given":"So","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Kanagawa, Japan"},{"name":"Micro\/Nano Technology Center, Tokai University, Hiratsuka 259-1292, Kanagawa, Japan"},{"name":"Institute of Medical Sciences, Tokai University, Isehara 259-1193, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2519-5874","authenticated-orcid":false,"given":"Shigeharu","family":"Moriya","sequence":"additional","affiliation":[{"name":"Photonics Control Technology Team, Riken Center for Advanced Photonics, Numazu 410-8601, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3798-3560","authenticated-orcid":false,"given":"Kazuhiko","family":"Imakawa","sequence":"additional","affiliation":[{"name":"Research Institute of Agriculture, Tokai University, Kumamoto 862-8652, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"ref_1","first-page":"141","article-title":"Genetic improvement of Japanese Brown cattle, the past, present and future","volume":"35","author":"Sumio","year":"2007","journal-title":"Jpn. 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