{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T23:29:13Z","timestamp":1771889353280,"version":"3.50.1"},"reference-count":60,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T00:00:00Z","timestamp":1720224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"U.S. Department of Agriculture National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2022-67015-36319"],"award-info":[{"award-number":["2022-67015-36319"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>During the periparturient period, cows undergo heightened energy demands at lactation onset, paired with reduced dry matter intake, leading to negative energy balance (NEB). Excessive lipolysis-driven adipose tissue remodeling, triggered by NEB, significantly contributes to ketosis in periparturient dairy cows. However, the role of peripheral blood mononuclear cells (PBMCs) in the pathogenesis of ketosis and in modulating adipose tissue function remains poorly understood. Here, we investigated how ketosis affects the transcriptional profile and secretome of PBMCs and its influence on preadipocyte function in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). Twenty-one postpartum Holstein dairy cows were categorized as either subclinical ketosis (SCK; BHB \u2265 1.0 mM) or control (CON; BHB &lt; 0.8 mM) based on blood beta-hydroxybutyrate (BHB) concentration screening. Blood samples were collected intravenously for the isolation of PBMCs and serum metabolic profiling. Ketosis elevated circulating NEFA and BHB levels but reduced total WBC and neutrophil counts. Isolated PBMCs were evaluated for gene expression and used to produce conditioned media (PBMC-CM), during which PBMCs were stimulated with 10 ng\/mL LPS. The overall phenotype of PBMCs was largely consistent between SCK and CON cows, with minimal differences detected in immunomodulatory cytokine expression and PBMC-CM composition following stimulation. Preadipocytes isolated from non-ketotic cows were treated with PBMC-CM to assess the effect of PBMC secretomes on adipose cell function. Preadipocytes treated with SCK PBMC-CM showed reduced lipid accumulation compared to those treated with CON PBMC-CM regardless of the depot. SAT preadipocytes had heightened expression of lipid metabolism-related genes, including DGAT1, LIPE, and FASN, compared to VAT when treated with SCK PBMC-CM. Preadipocytes treated with CM from PBMC stimulated by LPS exhibited upregulation in IL1B and IL6 regardless of the depot or source of PBMCs. Together, these results indicate that although PBMC profiles showed minimal differences, preadipocytes treated with PBMC-CM may be influenced by additional factors, leading to altered preadipocyte function and gene expression that may contribute to adipose cellular dysfunction.<\/jats:p>","DOI":"10.3390\/ani14131995","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T04:52:49Z","timestamp":1720414369000},"page":"1995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Effect of Subclinical Ketosis on the Peripheral Blood Mononuclear Cell Inflammatory Response and Its Crosstalk with Depot-Specific Preadipocyte Function in Dairy Cows"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9995-5752","authenticated-orcid":false,"given":"Hunter R.","family":"Ford","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ty M.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8403-9323","authenticated-orcid":false,"given":"Tanner","family":"Scull","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar J.","family":"Benitez","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA"},{"name":"School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8824-4023","authenticated-orcid":false,"given":"Clarissa","family":"Strieder-Barboza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA"},{"name":"School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.14202\/vetworld.2017.1367-1377","article-title":"Metabolic and immunological changes in transition dairy cows: A review","volume":"10","author":"Wankhade","year":"2017","journal-title":"Vet. World"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.3168\/jds.S0022-0302(80)83111-0","article-title":"Partitioning of nutrients during pregnancy and lactation: A review of mechanisms involving homeostasis and homeorhesis","volume":"63","author":"Bauman","year":"1980","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1083\/jcb.201409063","article-title":"The cell biology of fat expansion","volume":"208","author":"Rutkowski","year":"2015","journal-title":"J. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.3168\/jds.2010-3868","article-title":"Physiological and conjugated linoleic acid-induced changes of adipocyte size in different fat depots of dairy cows during early lactation","volume":"94","author":"Akter","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"E100","DOI":"10.3168\/jds.S0022-0302(01)70204-4","article-title":"Adaptations of glucose and long-chain fatty acid metabolism in liver of dairy cows during the periparturient period","volume":"84","author":"Drackley","year":"2001","journal-title":"J. Dairy Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6176","DOI":"10.3168\/jds.2015-9370","article-title":"Macrophage infiltration in the omental and subcutaneous adipose tissues of dairy cows with displaced abomasum","volume":"98","author":"Contreras","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1038\/nature05488","article-title":"Abdominal obesity and metabolic syndrome","volume":"444","author":"Lemieux","year":"2006","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Michelotti, T.C., Kisby, B.R., Flores, L.S., Tegeler, A.P., Fokar, M., Crasto, C., Menarim, B.C., Loux, S.C., and Strieder-Barboza, C. (2022). Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.1025240"},{"key":"ref_9","unstructured":"Ford, H.R., Michelotti, T., and Strieder-Barboza, C. (2024, January 20\u201323). Single-nuclei RNA Sequencing of adipose tissue of dairy cows with subclinical ketosis. Proceedings of the Conference of Research Workers in Animal Diseases, Chicago, IL, USA."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zeng, Y., David, J., R\u00e9mond, D., Dardevet, D., Savary-Auzeloux, I., and Polakof, S. (2018). Peripheral blood mononuclear cell metabolism acutely adapted to postprandial transition and mainly reflected metabolic adipose tissue adaptations to a high-fat diet in minipigs. Nutrients, 10.","DOI":"10.3390\/nu10111816"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kleiveland, C.R. (2015). Peripheral blood mononuclear cells. The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models, Springer.","DOI":"10.1007\/978-3-319-16104-4_15"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/1745039X.2015.1013666","article-title":"Effects of elevated parameters of subclinical ketosis on the immune system of dairy cows: In vivo and in vitro results","volume":"69","author":"Schulz","year":"2015","journal-title":"Arch. Anim. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.dci.2018.11.021","article-title":"The degree of postpartum metabolic challenge in dairy cows is associated with peripheral blood mononuclear cell transcriptome changes of the innate immune system","volume":"93","author":"Mann","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10059","DOI":"10.3168\/jds.2021-20136","article-title":"Effect of diet and nonesterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows","volume":"104","author":"Cheng","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vph.2009.12.004","article-title":"The role of chemokines in recruitment of immune cells to the artery wall and adipose tissue","volume":"52","author":"Surmi","year":"2010","journal-title":"Vasc. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.3168\/jds.S0022-0302(94)77212-X","article-title":"Principal descriptors of body condition score in Holstein cows","volume":"77","author":"Ferguson","year":"1994","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetpar.2010.03.038","article-title":"Generation of IFN-\u03b3-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines","volume":"171","author":"Yamaguchi","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kim, W.-S., Ghassemi Nejad, J., Roh, S.-G., and Lee, H.-G. (2020). Heat-shock proteins gene expression in peripheral blood mononuclear cells as an indicator of heat stress in beef calves. Animals, 10.","DOI":"10.3390\/ani10050895"},{"key":"ref_19","first-page":"964.1","article-title":"Effect of prepartum adiposity and lipolysis on gestational and postnatal adipose tissue inflammation and immune cell infiltration","volume":"31","author":"Lock","year":"2017","journal-title":"FASEB J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4628","DOI":"10.3168\/jds.2018-15808","article-title":"Fetuin-A modulates lipid mobilization in bovine adipose tissue by enhancing lipogenic activity of adipocytes","volume":"102","author":"Contreras","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.vetimm.2013.05.004","article-title":"Epigenetic regulation of the innate immune response to LPS in bovine peripheral blood mononuclear cells (PBMC)","volume":"154","author":"Doherty","year":"2013","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1042\/bj1070683","article-title":"Biochemical aspects of bovine ketosis","volume":"107","author":"Baird","year":"1968","journal-title":"Biochem. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.cvfa.2023.02.004","article-title":"Hyperketonemia: A Marker of Disease, a Sign of a High-Producing Dairy Cow, or Both?","volume":"39","author":"Mann","year":"2023","journal-title":"Vet. Clin. Food Anim. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"159","DOI":"10.14432\/j.macvetrev.2015.04.042","article-title":"Comparative clinical and haematological investigations in lactating cows with subclinical and clinical ketosis","volume":"38","author":"Marutsova","year":"2015","journal-title":"Maced. Vet. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Choi, W., Ro, Y., Choe, E., Hong, L., Kim, D., Kim, S., Yoon, I., and Kim, D. (2023). Comparison of prepartum blood parameters in dairy cows with postpartum ketosis and new risk prediction candidates. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1161596"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/s41598-022-04893-w","article-title":"Predicting ketosis during the transition period in Holstein Friesian cows using hematological and serum biochemical parameters on the calving date","volume":"12","author":"Ha","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.3168\/jds.2012-6035","article-title":"Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows","volume":"96","author":"Suthar","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.3168\/jds.2014-8237","article-title":"Diseases, reproductive performance, and changes in milk production associated with subclinical ketosis in dairy cows: A meta-analysis and review","volume":"97","author":"Raboisson","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.theriogenology.2014.04.014","article-title":"Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis","volume":"82","author":"Brodzki","year":"2014","journal-title":"Theriogenology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.rvsc.2005.05.002","article-title":"Changes in blood and milk lymphocyte sub-populations during acute and chronic phases of Staphylococcus aureus induced bovine mastitis","volume":"80","author":"Johannisson","year":"2006","journal-title":"Res. Vet. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/vcp.13069","article-title":"Hematology reference intervals during the prepartum period, first week after calving, and peak lactation in clinically healthy Holstein cows","volume":"51","author":"Tsiamadis","year":"2022","journal-title":"Vet. Clin. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"S155","DOI":"10.1093\/jas\/skaa134","article-title":"Interaction between inflammation and metabolism in periparturient dairy cows","volume":"98","author":"Mezzetti","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.3168\/jds.2015-10354","article-title":"Nutritional strategies to optimize dairy cattle immunity","volume":"99","author":"Sordillo","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"110182","DOI":"10.1016\/j.vetimm.2020.110182","article-title":"Association of metabolic markers with neutrophil function in healthy postpartum dairy cows","volume":"232","author":"Pascottini","year":"2021","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.3168\/jds.S0022-0302(04)73246-4","article-title":"Effects of nonesterified fatty acids on lymphocyte function in dairy heifers","volume":"87","author":"Lacetera","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"708","DOI":"10.3168\/jds.2011-4695","article-title":"Effect of postcalving serum nonesterified fatty acids concentration on the functionality of bovine immune cells","volume":"95","author":"Ster","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/ni.3153","article-title":"IL-6 as a keystone cytokine in health and disease","volume":"16","author":"Hunter","year":"2015","journal-title":"Nat. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1080\/1828051X.2021.2006806","article-title":"In vitro effects of nonesterified fatty acids and \u03b2-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes","volume":"20","author":"Li","year":"2021","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/imr.12616","article-title":"Interleukin-18: Biological properties and role in disease pathogenesis","volume":"281","author":"Kaplanski","year":"2018","journal-title":"Immunol. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1210\/er.2010-0018","article-title":"Emerging roles for the transforming growth factor-\u03b2 superfamily in regulating adiposity and energy expenditure","volume":"32","author":"Zamani","year":"2011","journal-title":"Endocr. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1083\/jcb.149.3.667","article-title":"Roles of autocrine TGF-\u03b2 receptor and Smad signaling in adipocyte differentiation","volume":"149","author":"Choy","year":"2000","journal-title":"J. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.2527\/jas1989.6782171x","article-title":"Transforming growth factor type \u03b2 (TGF-\u03b2) and adipogenesis in pigs","volume":"67","author":"Richardson","year":"1989","journal-title":"J. Anim. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Meng, Y., Gao, F., Xiao, Y., Zheng, Y., Wang, H.-Q., Gao, Y., Jiang, H., Yuan, B., and Zhang, J.-B. (2020). TGF-\u03b21-mediated FDNCR1 regulates porcine preadipocyte differentiation via the TGF-\u03b2 signaling pathway. Animals, 10.","DOI":"10.3390\/ani10081399"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"101086","DOI":"10.1016\/j.molmet.2020.101086","article-title":"Adipocytes fail to maintain cellular identity during obesity due to reduced PPAR\u03b3 activity and elevated TGF\u03b2-SMAD signaling","volume":"42","author":"Roh","year":"2020","journal-title":"Mol. Metab."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00335-023-10022-3","article-title":"Adipose tissue depot specific expression and regulation of fibrosis-related genes and proteins in experimental obesity","volume":"35","author":"Eisinger","year":"2024","journal-title":"Mamm. Genome"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1439-0396.2011.01130.x","article-title":"Coordinated gene expression in adipose tissue and liver differs between cows with high or low NEFA concentrations in early lactation","volume":"96","author":"Sadri","year":"2012","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ning, M., Zhao, Y., Li, Z., and Cao, J. (2022). Ketosis Alters Transcriptional Adaptations of Subcutaneous White Adipose Tissue in Holstein Cows during the Transition Period. Animals, 12.","DOI":"10.3390\/ani12172238"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5018","DOI":"10.3168\/jds.2022-22941","article-title":"Gene co-expression network and differential expression analyses of subcutaneous white adipose tissue reveal novel insights into the pathological mechanisms underlying ketosis in dairy cows","volume":"106","author":"Ning","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev.nutr.20.1.365","article-title":"Molecular mechanisms regulating hormone-sensitive lipase and lipolysis","volume":"20","author":"Holm","year":"2000","journal-title":"Annu. Rev. Nutr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/cvr\/cvae005","article-title":"Perilipin 1: A systematic review on its functions on lipid metabolism and atherosclerosis in mice and humans","volume":"120","author":"Desgrouas","year":"2024","journal-title":"Cardiovasc. Res."},{"key":"ref_51","first-page":"251","article-title":"The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surfaces of lipid storage droplets","volume":"1483","author":"Brasaemle","year":"2000","journal-title":"Biochim. Et Biophys. Acta (BBA)-Mol. Cell Biol. Lipids"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0165-5728(02)00019-X","article-title":"Stimulation with phytohaemagglutinin induces the synthesis of catecholamines in human peripheral blood mononuclear cells: Role of protein kinase C and contribution of intracellular calcium","volume":"125","author":"Cosentino","year":"2002","journal-title":"J. Neuroimmunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/S0301-472X(98)00057-5","article-title":"Endogenous catecholamine synthesis, metabolism, storage, and uptake in human peripheral blood mononuclear cells","volume":"27","author":"Marino","year":"1999","journal-title":"Exp. Hematol."},{"key":"ref_54","first-page":"3553","article-title":"Catecholamine content and in vitro catecholamine synthesis in peripheral human lymphocytes","volume":"81","author":"Musso","year":"1996","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Yu, H., Gao, X., Loor, J.J., Jiang, Q., Fang, Z., Hao, X., Shi, Z., Fan, M., Chen, M., and Li, X. (2022). Activation of Transcription Factor EB Is Associated with Adipose Tissue Lipolysis in Dairy Cows with Subclinical Ketosis. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.816064"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S0024-3205(00)00642-1","article-title":"In vivo lipolysis in adipose tissue from two anatomical locations measured by microdialysis","volume":"67","author":"Portillo","year":"2000","journal-title":"Life Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Queathem, E.D., Welly, R.J., Clart, L.M., Rowles, C.C., Timmons, H., Fitzgerald, M., Eichen, P.A., Lubahn, D.B., and Vieira-Potter, V.J. (2021). White adipose tissue depots respond to chronic beta-3 adrenergic receptor activation in a sexually dimorphic and depot divergent manner. Cells, 10.","DOI":"10.3390\/cells10123453"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1113\/jphysiol.2006.111484","article-title":"Functions of AMP-activated protein kinase in adipose tissue","volume":"574","author":"Daval","year":"2006","journal-title":"J. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1038\/s41423-019-0296-z","article-title":"NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling","volume":"18","author":"Unamuno","year":"2021","journal-title":"Cell. Mol. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"15967","DOI":"10.1038\/s41598-018-34186-0","article-title":"GSK3\u03b2 inhibition attenuates LPS-induced IL-6 expression in porcine adipocytes","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/13\/1995\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:11:03Z","timestamp":1760109063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/13\/1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,6]]},"references-count":60,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ani14131995"],"URL":"https:\/\/doi.org\/10.3390\/ani14131995","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,6]]}}}