{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:59:53Z","timestamp":1790017193791,"version":"4.0.1"},"reference-count":43,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of the current study was to evaluate the effects of soybean oil inclusion in diets on feeding behaviour, digestibility, performance, and blood metabolites of feedlot lambs. Forty non-castrated Santa In\u00eas lambs with a mean age of 5 months and initial body weight of 34.88 \u00b1 3.19 kg were used in a 40-day feeding trial. The lambs were distributed in five experimental diets with the inclusion of increasing soybean oil (SO) levels: 0, 30, 60, 90, and 120 g\/kg DM. The SO inclusion promoted a linear reduction in DM intake (p &lt; 0.001), crude protein (CP, p &lt; 0.001), non-fibrous carbohydrates (NFC, p &lt; 0.001), and total digestible nutrients (TDN, p = 0.004). There was an increasing quadratic effect on the intake of ether extract (EE; p = 0.002) and decreasing for neutral detergent fiber (p = 0.005). The soybean oil inclusion promoted the greater apparent digestibility of CP (p = 0.016), EE (p = 0.005), NDFom (p &lt; 0.001), and TDN (p &lt; 0.001); on the other hand, the apparent digestibility of NFC (p = 0.005) was decreased. The average daily gain decreased (p &lt; 0.001) with SO inclusion. The SO inclusion increased feeding time (p = 0.004), reduced the efficiency of DM rumination (p = 0.001), and reduced the concentration of blood N-ureic (p &lt; 0.001). Considering the productive parameters, SO can be included in diets and it is recommended that we include SO of up to 41 g\/kg DM in diets for fattening lambs as the ideal maximum level. The strategy implemented to adapt lambs to increasing levels of high-fat diet mitigated the detrimental effects of lipids on the rumen, with high-density energy intake being the constraining factor on performance.<\/jats:p>","DOI":"10.3390\/ani14142075","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T11:12:49Z","timestamp":1721128369000},"page":"2075","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Soybean Oil, Linoleic Acid Source, in Lamb Diets: Intake, Digestibility, Performance, Ingestive Behaviour, and Blood Metabolites"],"prefix":"10.3390","volume":"14","author":[{"given":"Victor G. O.","family":"Lima","sequence":"first","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane O.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E.","family":"de Freitas J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8916-2238","authenticated-orcid":false,"given":"Henry D. R.","family":"Alba","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willian P.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas dos S.","family":"Pina","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7781-1819","authenticated-orcid":false,"given":"Laud\u00ed C.","family":"Leite","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal do Rec\u00f4ncavo da Bahia, Cruz das Almas 44380000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8776-1336","authenticated-orcid":false,"given":"Carlindo S.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie A.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carly A.","family":"Becker","sequence":"additional","affiliation":[{"name":"Penn State Extension, College of Agricultural Science, Pen State University, State College, PA 16802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4108-6782","authenticated-orcid":false,"given":"Gleidson G. P.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador 40170110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121265","DOI":"10.1016\/j.lfs.2022.121265","article-title":"Understanding interactions among diet, host and gut microbiota for personalized nutrition","volume":"312","author":"Li","year":"2022","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Poli, A., Agostoni, C., and Visioli, F. (2023). Dietary Fatty acids and inflammation: Focus on the n-6 Series. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054567"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.anifeedsci.2013.09.016","article-title":"Growth, feed intake, carcass characteristics, and meat fatty acid profile of lambs fed soybean oil partially replaced by fish oil blend","volume":"187","author":"Ferreira","year":"2014","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yang, A., Zhang, C., Zhang, B., Wang, Z., Zhu, L., Mu, Y., Wang, S., and Qi, D. (2021). Effects of dietary cottonseed oil and cottonseed meal supplementation on liver lipid content, fatty acid profile and hepatic function in laying hens. Animals, 11.","DOI":"10.3390\/ani11010078"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Nascimento, C.O., Pina, D.S., Cirne, L.G.A., Santos, S.A., Ara\u00fajo, M.L.G.M.L., Rodrigues, T.C.G.C., Silva, W.P., Souza, M.N.S., Alba, H.D.R., and Carvalho, G.G.P. (2021). Effects of whole corn germ, a source of linoleic acid, on carcass characteristics and meat quality of feedlot lambs. Animals, 11.","DOI":"10.3390\/ani11020267"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Oliveira, M.X.S., Palma, A.S., Reis, B.R., Franco, C.S., Marconi, A.P., Shiozaki, F.A., Reis, L.G., Salles, M.S.V., and Netto, A.S. (2021). Inclusion of soybean and linseed oils in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human diet. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0246357"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.anifeedsci.2015.09.007","article-title":"Nutrient digestibility and ruminal fatty acid metabolism in lambs supplemented with soybean oil partially replaced by fish oil blend","volume":"216","author":"Ferreira","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.smallrumres.2017.03.004","article-title":"Influence of dietary oils on performance, blood metabolites, purine derivatives, cellulase activity and muscle fatty acid composition in fattening lambs","volume":"150","author":"Parvar","year":"2017","journal-title":"Small Rumin. Res."},{"key":"ref_9","unstructured":"NRC\u2014National Research Council (2007). Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids, National Academic Press."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"s272","DOI":"10.1017\/S1751731118001994","article-title":"Modulating ruminal lipid metabolism to improve the fatty acid composition of meat and milk. Challenges and opportunities","volume":"12","author":"Toral","year":"2018","journal-title":"Animal"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e91721","DOI":"10.1590\/fst.91721","article-title":"The lipid source can modify saturated and unsaturated fatty acids profile of meat of lambs","volume":"42","author":"Rodrigues","year":"2022","journal-title":"Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3875","DOI":"10.2527\/jas.2016-0598","article-title":"Feeding value of supplemental Jatropha curcas crude oil in finishing diets for feedlot lambs","volume":"94","author":"Barreras","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bahramkhani-Zaringoli, L., Mirzaei-Alamouti, H., Aschenbach, J.R., Vazirigohar, M., Patra, A.K., Jafari-Anarkooli, I., Ganjkhanlou, M., Alipour, D., and Mansouryar, M. (2022). Effects of oil supplements on growth performance, eating behaviour, ruminal fermentation, and ruminal morphology in lambs during transition from a low-to a high-grain diet. Animals, 12.","DOI":"10.3390\/ani12192566"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.smallrumres.2016.03.012","article-title":"Feeding behaviour, nutrient digestibility, feedlot performance, carcass traits, and meat characteristics of crossbred lambs fed high levels of yellow grease or soybean oil","volume":"137","author":"Ezequiel","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.meatsci.2014.10.014","article-title":"Growth performance, carcass and meat quality of lambs supplemented with increasing levels of a tanniferous bush (Cistus ladanifer L.) and vegetable oils","volume":"100","author":"Francisco","year":"2015","journal-title":"Meat Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.anifeedsci.2019.01.017","article-title":"Dietary supplementation with tannin and soybean oil on intake, digestibility, feeding behaviour, ruminal protozoa and methane emission in sheep","volume":"249","author":"Lima","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"107971","DOI":"10.1016\/j.meatsci.2019.107971","article-title":"Effects of the dietary inclusion of babassu oil or buriti oil on lamb performance, meat quality and fatty acid composition","volume":"160","author":"Parente","year":"2020","journal-title":"Meat Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.smallrumres.2016.06.012","article-title":"Effect of dietary oil sources on fatty acid composition of ruminal digesta and populations of specific bacteria involved in hydrogenation of 18-carbon unsaturated fatty acid in finishing lambs","volume":"144","author":"Zhao","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7881","DOI":"10.3168\/jds.2017-13666","article-title":"Ruminal biohydrogenation and abomasal flow of fatty acids in lactating cows fed diets supplemented with soybean oil, whole soybeans, or calcium salts of fatty acids","volume":"101","author":"Freitas","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1017\/S1751731119003215","article-title":"Control of feed intake by hepatic oxidation in ruminant animals: Integration of homeostasis and homeorhesis","volume":"14","author":"Allen","year":"2020","journal-title":"Animal"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"933","DOI":"10.3168\/jds.S0022-0302(91)78243-X","article-title":"Effects of prepartum diet, inert rumen bulk, and dietary polythylene glicol on dry matter intake of lactating dairy cows","volume":"74","author":"Johnson","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_22","first-page":"987","article-title":"Rumination of bovine and bubaline steers in feedlot regime","volume":"25","author":"Polli","year":"1996","journal-title":"Rev. Bras. Zootec."},{"key":"ref_23","first-page":"236","article-title":"Ingestive behaviour in Holstein calves fed diets with different concentrate levels","volume":"29","author":"Pereira","year":"2000","journal-title":"Rev. Bras. Zootec."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.anifeedsci.2017.08.013","article-title":"Comparing sheep and cattle to quantify internal markers in tropical feeds using in situ ruminal incubation","volume":"232","author":"Reis","year":"2017","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_25","unstructured":"AOAC\u2014Association of Official Analytical Chemists (1990). Official Methods of Analysis of the Association of Official Analytical Chemists, Association of Official Analytical Chemists. [15th ed.]."},{"key":"ref_26","first-page":"1217","article-title":"Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: Collaborative study","volume":"85","author":"Mertens","year":"2002","journal-title":"J. AOAC Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0377-8401(95)00837-3","article-title":"Standardization of procedures for nitrogen fractionation of ruminants feeds","volume":"57","author":"Licitra","year":"1996","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_28","unstructured":"Hall, M.B. (2000). Calculation of Non-Structural Carbohydrate Content of Feeds That Contain Non-Protein Nitrogen, University of Florida."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104532","DOI":"10.1016\/j.livsci.2021.104532","article-title":"Estimating digestible nutrients in diets for small ruminants fed with tropical forages","volume":"249","author":"Cruz","year":"2021","journal-title":"Livest. Sci."},{"key":"ref_30","unstructured":"NRC\u2014National Research Council (2001). Nutrient Requirements of Dairy Cattle, National Academic Press. [7th ed.]."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.2527\/jas.2006-491","article-title":"A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs","volume":"85","author":"Busboom","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s11745-997-0156-3","article-title":"Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids","volume":"32","author":"Kramer","year":"1997","journal-title":"Lipids"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.3168\/jds.2016-12165","article-title":"The effects of fructose and phosphate infusions on dry matter intake of lactating cows","volume":"100","author":"Yair","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.3168\/jds.2011-4991","article-title":"Hypophagic effects of propionate increase with elevated hepatic acetyl coenzyme A concentration for cows in the early postpartum period","volume":"95","author":"Stocks","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.2527\/2004.82102985x","article-title":"Soybean oil supplementation of a high-concentrate diet does not affect site and extent of organic matter, starch, neutral detergent fibre, or nitrogen digestion, but influences both ruminal metabolism and intestinal flow of fatty acids in limit-fed lambs","volume":"82","author":"Kucuk","year":"2004","journal-title":"J. Anim. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1071\/AN14478","article-title":"Drives and limits to feed intake in ruminants","volume":"54","author":"Allen","year":"2014","journal-title":"Anim. Prod. Sci."},{"key":"ref_37","first-page":"1952","article-title":"Effects of crude glycerin from biodiesel on the diets of lambs: Intake, digestibility, performance, feeding behaviour, and serum metabolites","volume":"96","author":"Ribeiro","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4025\/actascianimsci.v34i3.12729","article-title":"Effects of different lipid levels on protozoa population, microbial protein synthesis and rumen degradability in cattle","volume":"34","author":"Messana","year":"2012","journal-title":"Acta Sci. Anim. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.2527\/jas.2009-1779","article-title":"Board-invited review: The hepatic oxidation theory of the control of feed intake and its application to ruminants","volume":"87","author":"Allen","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_40","unstructured":"Kaneko, J.J., Harvery, J.W., and Bruss, M.L. (1997). Clinical Biochemistry of Domestic Animals, Academic Press. [5th ed.]."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1093\/jas\/sky086","article-title":"Effects of dietary protein sources and nisin on rumen fermentation, nutrient digestion, plasma metabolites, nitrogen utilization, and growth performance in growing lambs","volume":"96","author":"Shen","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.smallrumres.2013.12.025","article-title":"Effect of adding palm oil to the diet of dairy sheep on milk production and composition, function of liver and kidney, and the concentration of cholesterol, triglycerides and progesterone in blood serum","volume":"117","author":"Bianchi","year":"2014","journal-title":"Small Rumin. Res."},{"key":"ref_43","first-page":"143","article-title":"Relationships between liver ureagenesis and gluconeogenesis in ruminants fed with a high nitrogen diet","volume":"43","author":"Noro","year":"2012","journal-title":"Vet. M\u00e9x."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/14\/2075\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:17:24Z","timestamp":1760109444000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/14\/2075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,16]]},"references-count":43,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ani14142075"],"URL":"https:\/\/doi.org\/10.3390\/ani14142075","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,16]]}}}