{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:09:19Z","timestamp":1785510559871,"version":"3.56.0"},"reference-count":57,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011501","name":"Construction of Standardized Production System for Improving quality and efficiency of Tibetan sheep industry","doi-asserted-by":"publisher","award":["2022-NK-169"],"award-info":[{"award-number":["2022-NK-169"]}],"id":[{"id":"10.13039\/501100011501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011501","name":"Construction of Standardized Production System for Improving quality and efficiency of Tibetan sheep industry","doi-asserted-by":"publisher","award":["2022-NK-169-3"],"award-info":[{"award-number":["2022-NK-169-3"]}],"id":[{"id":"10.13039\/501100011501","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research and Integration of Production Technology for Special Tibetan Sheep in High Saline and Alkaline Habita","award":["2022-NK-169"],"award-info":[{"award-number":["2022-NK-169"]}]},{"name":"Research and Integration of Production Technology for Special Tibetan Sheep in High Saline and Alkaline Habita","award":["2022-NK-169-3"],"award-info":[{"award-number":["2022-NK-169-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Substituting traditional protein feed with palm kernel meal (PKM) in the diet of Tibetan sheep can be a cost-effective feeding strategy. To determine the impact of PKM on flavor development in different adipose tissues of Tibetan sheep, subjects were fed with 15% and 18% of PKM, while the control group received no PKM. The fatty acids and volatile compounds in the samples were then analyzed by GC-MS and HS-GC-IMS. Adding PKM to the diet significantly increased the C12:0, C14:0, C16:0 and C18:1N9 content in adipose tissues compared with the control, and most of these were associated with flavor formation (p &lt; 0.05). The flavor compounds in the adipose tissues predominantly consisted of alcohols, ketones, acids and aldehydes. In particular, including PKM in the diet increased the proportion of ketones but decreased the proportion of alcohols, acids and aldehydes in subcutaneous and tail fat. Specifically, the proportion of acetone, acetoin monomer, 2,3-butanedione, 2-butanone monomer, 2-methyl-2-propanol, 2-methyl-2-propanol and methyl acetate increased significantly in the subcutaneous and tail fat (p &lt; 0.05), while that of ethanol, 1-propanol monomer, butanol monomer, acetic acid monomer and acetic acid monomer decreased. Intermuscular fat exhibited variable results, mainly because the addition of PKM resulted in higher proportions of alcohols, including ethanol, 1-propanol and butanol monomer, especially at 15% PKM. In summary, the addition of PKM improved the flavor of Tibetan sheep fat and increased the amount of favorable volatile flavor compounds. This study can serve as reference for understanding the effects of dietary PKM on the adipose tissue flavor profile of Tibetan sheep.<\/jats:p>","DOI":"10.3390\/ani14142113","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T08:24:59Z","timestamp":1721636699000},"page":"2113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Fatty Acids and Volatile Flavor Components of Adipose Tissue from Local Tibetan Sheep in Qinghai with Dietary Supplementation of Palm Kernel Meal (PKM)"],"prefix":"10.3390","volume":"14","author":[{"given":"Ying","family":"Ma","sequence":"first","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Han","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengzhen","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linsheng","family":"Gui","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyou","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baochun","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103854","DOI":"10.1016\/j.livsci.2019.103854","article-title":"Oat hay as winter feed improves digestibility, nitrogen balance and energy utilization of Tibetan sheep (Ovis aries) in the Qinghai Tibetan Plateau","volume":"230","author":"Abdelraheem","year":"2019","journal-title":"Livest. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"131611","DOI":"10.1016\/j.foodchem.2021.131611","article-title":"Effects of different feeding regimes on muscle metabolism and its association with meat quality of Tibetan sheep","volume":"374","author":"Zhang","year":"2022","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"136666","DOI":"10.1016\/j.foodchem.2023.136666","article-title":"Identification of muttony-related compounds in cooked mutton tallows and their flavor intensities subjected to phenolic extract from thyme (Thymus vulgaris L.)","volume":"427","author":"Wu","year":"2023","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0309-1740(92)90024-X","article-title":"Distribution of volatile branched-chain fatty acids in various lamb tissues","volume":"31","author":"Brennand","year":"1992","journal-title":"Meat Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Teixeira, A., Silva, S., Guedes, C., and Rodrigues, S. (2020). Sheep and goat meat processed products quality: A review. Foods, 9.","DOI":"10.3390\/foods9070960"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1021\/jf303768e","article-title":"Sheepmeat flavor and the effect of different feeding systems: A review","volume":"61","author":"Watkins","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","unstructured":"Melton, L., Shahidi, F., and Varelis, P. (2019). Processing Effects on Meat Flavor. Encyclopedia of Food Chemistry, Academic Press."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1186\/s12944-018-0860-z","article-title":"Ruminant meat flavor influenced by different factors with special reference to fatty acids","volume":"17","author":"Arshad","year":"2018","journal-title":"Lipids Health Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106956","DOI":"10.1016\/j.smallrumres.2023.106956","article-title":"Adipose tissues of fat-tailed sheep reared in highland or lowland: Fatty acids and volatile compounds","volume":"222","author":"Dursun","year":"2023","journal-title":"Small Rumin. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.meatsci.2010.03.003","article-title":"Different oilseed supplements alter fatty acid composition of different adipose tissues of adult ewes","volume":"85","author":"Peng","year":"2010","journal-title":"Meat Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.meatsci.2004.11.008","article-title":"Fatty acid composition and carcass characteristics of growing lambs fed diets containing palm oil supplements","volume":"69","author":"Castro","year":"2005","journal-title":"Meat Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.aninu.2018.05.006","article-title":"Comparison of fatty acid profile of three adipose tissues in Ningxiang pigs","volume":"4","author":"Jiang","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108086","DOI":"10.1016\/j.meatsci.2020.108086","article-title":"Influence of dietary cardoon meal on volatile compounds and flavour in lamb meat","volume":"163","author":"Natalello","year":"2020","journal-title":"Meat Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.meatsci.2014.06.008","article-title":"Influence of fresh alfalfa supplementation on fat skatole and indole concentration and chop odour and flavour in lambs grazing a cocksfoot pasture","volume":"98","author":"Devincenzi","year":"2014","journal-title":"Meat Sci."},{"key":"ref_15","first-page":"26","article-title":"Determining the appropriate improvement methods for the pastures of eastern Anatolia region of Turkey","volume":"40","author":"Kokten","year":"2019","journal-title":"Range Manag. Agrofor."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100502","DOI":"10.1016\/j.aqrep.2020.100502","article-title":"Dietary inclusion of non-conventional palm kernel meal enhances growth, digestive enzyme activities and carcass characteristics of juvenile rohu (Labeo rohita)","volume":"18","author":"Sangavi","year":"2020","journal-title":"Aquac. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115213","DOI":"10.1016\/j.anifeedsci.2022.115213","article-title":"Improving fiber utilization from rapeseed and sunflower seed meals to substitute soybean meal in pig and chicken diets: A review","volume":"285","author":"Lannuzel","year":"2022","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3516","DOI":"10.1002\/fsn3.3340","article-title":"Exploring the effects of palm kernel meal feeding on the meat quality and rumen microorganisms of Qinghai Tibetan sheep","volume":"11","author":"Ma","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_19","unstructured":"Toldr\u00e1, F. (2023). Chapter 13\u2014The eating quality of meat: III\u2014Flavor. Lawrie\u2019s Meat Science, Woodhead Publishing. [9th ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"100843","DOI":"10.1016\/j.fochx.2023.100843","article-title":"Insight into the differences of meat quality between Qinghai white Tibetan sheep and black Tibetan sheep from the perspective of metabolomics and rumen microbiota","volume":"19","author":"Ma","year":"2023","journal-title":"Food Chem. X"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0921-4488(00)00128-0","article-title":"Fatty acid composition of goat muscles and fat depots: A review","volume":"37","author":"Banskalieva","year":"2000","journal-title":"Small Rumin. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4634","DOI":"10.1016\/j.psj.2020.05.052","article-title":"The body fat distribution and fatty acid composition of muscles and adipose tissues in geese","volume":"99","author":"Yu","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.meatsci.2009.08.029","article-title":"Red meat consumption: An overview of the risks and benefits","volume":"84","author":"McAfee","year":"2010","journal-title":"Meat Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105142","DOI":"10.1016\/j.livsci.2022.105142","article-title":"Characteristics, lipogenic enzyme activity, and fatty acid composition of muscles in the Iberian pig: Effects of protein restriction and free-range feeding","volume":"267","author":"Tejeda","year":"2023","journal-title":"Livest. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108944","DOI":"10.1016\/j.meatsci.2022.108944","article-title":"Feeding silkworm (Bombyx mori L.) oil to growing rabbits improves the fatty acid composition of meat, liver and perirenal fat","volume":"193","author":"Singh","year":"2022","journal-title":"Meat Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.anifeedsci.2014.06.007","article-title":"Impact of dietary fat sources and feeding level on adipose tissue fatty acids composition and lipid metabolism related gene expression in finisher pigs","volume":"196","author":"Kim","year":"2014","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.fct.2006.10.009","article-title":"Safety assessment of myristic acid as a food ingredient","volume":"45","author":"Burdock","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.earlhumdev.2017.08.009","article-title":"Myristic acid in amniotic fluid produces appetitive responses in human newborns","volume":"115","author":"Contreras","year":"2017","journal-title":"Early Hum. Dev."},{"key":"ref_29","unstructured":"Mariod, A.A. (2022). Chapter 30\u2014Chemistry and composition of coconut oil and its biological activities. Multiple Biological Activities of Unconventional Seed Oils, Academic Press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109138","DOI":"10.1016\/j.meatsci.2023.109138","article-title":"Altering the ratio of palmitic, stearic, and oleic acids in dietary fat affects nutrient digestibility, plasma metabolites, growth performance, carcass, meat quality, and lipid metabolism gene expression of Angus bulls","volume":"199","author":"Bai","year":"2023","journal-title":"Meat Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"114623","DOI":"10.1016\/j.anifeedsci.2020.114623","article-title":"Ability of tannins to modulate ruminal lipid metabolism and milk and meat fatty acid profiles","volume":"269","author":"Frutos","year":"2020","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.livsci.2017.02.021","article-title":"Comparison of fatty acid profiles and volatile compounds among quality grades and their association with carcass characteristics in longissimus dorsi and semimembranosus muscles of Korean cattle steer","volume":"198","author":"Piao","year":"2017","journal-title":"Livest. Sci."},{"key":"ref_33","first-page":"201","article-title":"An overview of the modulatory effects of oleic acid in health and disease","volume":"13","year":"2013","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_34","unstructured":"Dikeman, M. (2024). Flavor development in beef, pork, lamb and goat meat. Encyclopedia of Meat Sciences, Elsevier. [3rd ed.]."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Urli\u0107, M.A.-O., Urli\u0107, I., Urli\u0107, H., Ma\u0161ek, T., Benzon, B., Vitlov Uljevi\u0107, M., Vukojevi\u0107, K., and Filipovi\u0107, N.A.-O. (2020). Effects of Different n6\/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy. Nutrients, 12.","DOI":"10.3390\/nu12092761"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s11745-003-1067-z","article-title":"Fatty acids, the immune response, and autoimmunity: A question of n-6 essentiality and the balance between n-6 and n-3","volume":"38","author":"Harbige","year":"2003","journal-title":"Lipids"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"115297","DOI":"10.1016\/j.lwt.2023.115297","article-title":"Insight into the effect of microwave treatment on fat loss, fatty acid composition and microstructure of pork subcutaneous back fat","volume":"187","author":"Lian","year":"2023","journal-title":"LWT"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/S0308-8146(98)00076-4","article-title":"Flavour formation in meat and meat products: A review","volume":"62","author":"Mottram","year":"1998","journal-title":"Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.meatsci.2014.12.005","article-title":"Effects of dietary fat saturation on fatty acid composition and gene transcription in different tissues of Iberian pigs","volume":"102","author":"Isabel","year":"2015","journal-title":"Meat Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.meatsci.2006.10.013","article-title":"Comparison of the lipid content and fatty acid composition of intermuscular and subcutaneous adipose tissues in pig carcasses","volume":"76","author":"Monziols","year":"2007","journal-title":"Meat Sci."},{"key":"ref_41","unstructured":"Dikeman, M. (2024). Measuring meat flavour. Encyclopedia of Meat Sciences, Elsevier. [3rd ed.]."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.meatsci.2007.04.016","article-title":"A fresh look at meat flavor","volume":"77","author":"Calkins","year":"2007","journal-title":"Meat Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/1750-3841.13996","article-title":"Contribution of Histidine and Lysine to the Generation of Volatile Compounds in Jinhua Ham Exposed to Ripening Conditions Via Maillard Reaction","volume":"83","author":"Zhu","year":"2018","journal-title":"J. Food Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110222","DOI":"10.1016\/j.foodres.2021.110222","article-title":"GC \u00d7 GC-ToF-MS and GC-IMS based volatile profile characterization of the Chinese dry-cured hams from different regions","volume":"142","author":"Li","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1007\/s11694-019-00158-9","article-title":"Physical properties, compositions and volatile profiles of Chinese dry-cured hams from different regions","volume":"14","author":"Li","year":"2020","journal-title":"J. Food Meas. Charact."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"107904","DOI":"10.1016\/j.meatsci.2019.107904","article-title":"A metabolomics-based approach investigates volatile flavor formation and characteristic compounds of the Dahe black pig dry-cured ham","volume":"158","author":"Shi","year":"2019","journal-title":"Meat Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"100871","DOI":"10.1016\/j.fochx.2023.100871","article-title":"The regulation of key flavor of traditional fermented food by microbial metabolism: A review","volume":"19","author":"Zhang","year":"2023","journal-title":"Food Chem. X"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"131103","DOI":"10.1016\/j.foodchem.2021.131103","article-title":"Changes in volatile flavor of yak meat during oxidation based on multi-omics","volume":"371","author":"Huang","year":"2022","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10068-015-0048-5","article-title":"Detection of volatile organic compounds as markers of chicken breast spoilage using HS-SPME-GC\/MS-FASST","volume":"24","author":"Yoon","year":"2015","journal-title":"Food Sci. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Poomani, M.S., Mariappan, I., Muthan, K., and Subramanian, V. (2023). A thermotolerant yeast from cow\u2019s rumen utilize lignocellulosic biomass from wheat straw for xylanase production and fermentation to ethanol. Biocatal. Agric. Biotechnol., 50.","DOI":"10.1016\/j.bcab.2023.102741"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.renene.2018.09.100","article-title":"Cellulolytic and xylanolytic enzymes of thermophiles for the production of renewable biofuels","volume":"136","author":"Bala","year":"2019","journal-title":"Renew. Energy"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104041","DOI":"10.1016\/j.livsci.2020.104041","article-title":"Effects of increasing levels of palm kernel meal containing \u03b2-mannanase to growing-finishing pig diets on growth performance, nutrient digestibility, and pork quality","volume":"238","author":"Jang","year":"2020","journal-title":"Livest. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"100494","DOI":"10.1016\/j.fochx.2022.100494","article-title":"Exploring the formation mechanism of off-flavor of irradiated yak meat based on metabolomics","volume":"16","author":"Wang","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101","DOI":"10.26599\/FSHW.2022.9250008","article-title":"Insights into microbiota community dynamics and flavor development mechanism during golden pomfret (Trachinotus ovatus) fermentation based on single-molecule real-time sequencing and molecular networking analysis","volume":"13","author":"Wang","year":"2024","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"106398","DOI":"10.1016\/j.smallrumres.2021.106398","article-title":"Branched chain fatty acids in the flavour of sheep and goat milk and meat: A review","volume":"200","author":"Watkins","year":"2021","journal-title":"Small Rumin. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1007\/s00253-007-1288-y","article-title":"Formation of volatile sulfur compounds and metabolism of methionine and other sulfur compounds in fermented food","volume":"77","author":"Landaud","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_57","first-page":"1227","article-title":"Changes of flavor components of Changle midge son shrimp in fermentation process","volume":"8","author":"Chen","year":"2017","journal-title":"J. Food Saf. Qual."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/14\/2113\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:20:08Z","timestamp":1760109608000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/14\/2113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,20]]},"references-count":57,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ani14142113"],"URL":"https:\/\/doi.org\/10.3390\/ani14142113","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,20]]}}}