{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:55:21Z","timestamp":1792803321915,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Program of Khon Kaen University, Thailand","award":["RP67-3-003"],"award-info":[{"award-number":["RP67-3-003"]}],"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Research Program of Khon Kaen University, Thailand","award":["651T102"],"award-info":[{"award-number":["651T102"]}],"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"crossref"}]},{"name":"graduate school of Khon Kaen University","award":["RP67-3-003"],"award-info":[{"award-number":["RP67-3-003"]}],"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"crossref"}]},{"name":"graduate school of Khon Kaen University","award":["651T102"],"award-info":[{"award-number":["651T102"]}],"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The cooling of semen storage at 5 \u00b0C from a Thai native rooster (Pradu Hang Dum), supplemented with herbs possessing antioxidant properties, provided limited research. This study was conducted to evaluate the efficiency of Aloe vera (AV) gel supplementation at various levels on the quality of cooled semen and subsequent fertility after artificial insemination. Sixty-four chickens had semen pooled, diluted, and supplemented with different levels of AV gel (0% as control, 0.25%, 0.50%, 1.0%, 2.5%, 5.0%, 10%, and 20%), and then stored for 72 h. In Experiment 1, semen quality, malondialdehyde (MDA) levels, and pH values were assessed at 0, 24, 48, and 72 h after storage. Experiment 2 assessed fertility potential using the most effective cooled storage semen from Experiment 1. Results showed a decrease in semen quality with prolonged storage time (p &lt; 0.001). The highest semen quality was observed in the group supplemented with 1.0% AV gel (p &lt; 0.001), whereas the lowest was noted in the 20% AV gel group (p &lt; 0.001). Furthermore, the 1.0% AV gel group exhibited the highest semen quality at 24, 48, and 72 h of storage. The evaluation of fertility and hatchability rates revealed a statistically significant improvement in fertility potential (p &lt; 0.05) in the group supplemented with 1.0% AV gel. In summary, this study represents the first investigation of stored Thai native rooster semen using a semen extender supplemented with Aloe vera gel at 5 \u00b0C, demonstrating its efficacy for storage up to 72 h. The addition of 1% AV gel was recommended as an antioxidant supplementation during the semen storage process at 5 \u00b0C to enhance semen quality and fertility rates.<\/jats:p>","DOI":"10.3390\/ani14162290","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T15:24:16Z","timestamp":1722957856000},"page":"2290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Unveiling the Potential of Aloe vera Gel Supplementation in a Cooling Extender: A Breakthrough in Enhancing Rooster Sperm Quality and Fertility Ability"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4712-4399","authenticated-orcid":false,"given":"Jutarat","family":"Pimpa","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7907-5173","authenticated-orcid":false,"given":"Supakorn","family":"Authaida","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-2300","authenticated-orcid":false,"given":"Wuttigrai","family":"Boonkum","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1572-4389","authenticated-orcid":false,"given":"Sarinya","family":"Rerkyusuke","sequence":"additional","affiliation":[{"name":"Division of Livestock Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0011-8511","authenticated-orcid":false,"given":"Chalinee","family":"Janta","sequence":"additional","affiliation":[{"name":"Medicinal Plants Innovation Center of Mae Fah Luang University, Chiang Rai 57100, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3619-8680","authenticated-orcid":false,"given":"Vibuntita","family":"Chankitisakul","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"833","DOI":"10.5851\/kosfa.2022.e35","article-title":"The Comparison between Tanzanian Indigenous (Ufipa Breed) and Commercial Broiler (Ross Chicken) Meat on the Physicochemical Characteristics, Collagen and Nucleic Acid Contents","volume":"42","author":"Mussa","year":"2022","journal-title":"Food Sci. Anim. Resour."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Juiputta, J., Chankitisakul, V., and Boonkum, W. (2023). Appropriate Genetic Approaches for Heat Tolerance and Maintaining Good Productivity in Tropical Poultry Production: A Review. Vet. Sci., 10.","DOI":"10.3390\/vetsci10100591"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"101101","DOI":"10.1016\/j.psj.2021.101101","article-title":"Effect of Different Rearing System on Eggs Production, Hatchability, and Offspring Quality in Layer Breeders","volume":"100","author":"Damaziak","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Bekele, F., Tafese, L., Demsash, A.W., Tesfaye, H., Labata, B.G., and Fekadu, G. (2023). Adherence to Self-Care Practices and Associated Factors among Heart Failure Patients in Ethiopia: A Systematic Review and Meta-Analysis. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0288824"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1080\/00071667808416462","article-title":"Preservation of Fowl Semen in Liquid Nitrogen\u2014An Improved Method","volume":"19","author":"Lake","year":"1978","journal-title":"Br. Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Gibb, Z., and Aitken, R.J. (2016). The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model. BioMed Res. Int., 2016.","DOI":"10.1155\/2016\/9380609"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102188","DOI":"10.1016\/j.psj.2022.102188","article-title":"Fertilizing Ability and Survivability of Rooster Sperm Diluted with a Novel Semen Extender Supplemented with Serine for Practical Use on Smallholder Farms","volume":"101","author":"Chankitisakul","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"513","DOI":"10.4081\/ijas.2005.2s.513","article-title":"Comparison of Different Extenders on the Quality Characteristics of Turkey Semen during Storage","volume":"4","author":"Iaffaldano","year":"2005","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-4320(00)00160-3","article-title":"Storage of Poultry Semen","volume":"62","author":"Donoghue","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_10","first-page":"53","article-title":"Application of Aloe vera on Wound Healing","volume":"22","author":"Tangjitjareonkun","year":"2015","journal-title":"J. Med. Health Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1590\/S1517-83822009000400023","article-title":"Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera","volume":"40","author":"Lawrence","year":"2009","journal-title":"Braz. J. Microbiol. Publ. Braz. Soc. Microbiol."},{"key":"ref_12","first-page":"8","article-title":"Effect of Aloe vera Extracts on in Vitro Human Sperm Parameters for Asthenozoospermic Patients","volume":"5","author":"Baqir","year":"2014","journal-title":"J. Thi-Qar Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.theriogenology.2016.01.007","article-title":"Use of Aloe vera-Based Extender for Chilling and Freezing Collared Peccary (Pecari tajacu) Semen","volume":"85","author":"Souza","year":"2016","journal-title":"Theriogenology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.20546\/ijcmas.2020.909.202","article-title":"Antioxidant Effects of Aloe vera as Semen Additive in Cryopreservation of Cattle Bull Semen","volume":"9","author":"Agarwal","year":"2020","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"ref_15","first-page":"10","article-title":"In Vitro Antioxidant Activity, Total Phenolic and Flavonoid Contents of Ethanol Extract of Stem and Leaf of Grewia carpinifolia","volume":"6","author":"Adebiyi","year":"2017","journal-title":"Beni-Suef Univ. J. Basic Appl. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"984295","DOI":"10.1155\/2012\/984295","article-title":"Phytochemical Characteristics, Free Radical Scavenging Activities, and Neuroprotection of Five Medicinal Plant Extracts","volume":"2012","author":"Chang","year":"2012","journal-title":"Evid. Based Complement. Altern. Med. ECAM"},{"key":"ref_17","first-page":"93","article-title":"Spectrophotometric Estimation of Total Polysaccharides in Cassia Tora Gum","volume":"30","author":"Pawar","year":"2011","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Xiao, X., Ren, W., Zhang, N., Bing, T., Liu, X., Zhao, Z., and Shangguan, D. (2019). Comparative Study of the Chemical Constituents and Bioactivities of the Extracts from Fruits, Leaves and Root Barks of Lycium Barbarum. Molecules, 24.","DOI":"10.3390\/molecules24081585"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1079\/WPS19960003","article-title":"Poultry Artificial Insemination Technology in the Countries of the Former USSR","volume":"52","author":"Surai","year":"1996","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.rvsc.2008.03.010","article-title":"Efficacy of Evaluation of Rooster Sperm Morphology Using Different Staining Methods","volume":"85","author":"Lukaszewicz","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cryobiol.2020.12.008","article-title":"Effect of Organic Selenium Dietary Supplementation on Quality and Fertility of Cryopreserved Chicken Sperm","volume":"98","author":"Thananurak","year":"2021","journal-title":"Cryobiology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.mtchem.2018.05.001","article-title":"Role of Plant Alkaloids on Human Health: A Review of Biological Activities","volume":"9","author":"Debnath","year":"2018","journal-title":"Mater. Today Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6123850","DOI":"10.1155\/2018\/6123850","article-title":"Chemical Composition and Antioxidant Activity of Aloe vera from the Pica Oasis (Tarapac\u00e1, Chile) by UHPLC-Q\/Orbitrap\/MS\/MS","volume":"2018","author":"Quispe","year":"2018","journal-title":"J. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/978-3-030-71697-4_15","article-title":"Cinnamon: A Promising Natural Product Against COVID-19","volume":"1327","author":"Zareie","year":"2021","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1093\/glycob\/cwh050","article-title":"Chemical and Biological Characterization of a Polysaccharide Biological Response Modifier from Aloe vera L. Var. chinensis (Haw.) Berg.","volume":"14","author":"Leung","year":"2004","journal-title":"Glycobiology"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Li, Y., Liang, W., Han, Y., Zhao, W., Wang, S., and Qin, C. (2022). Triterpenoids and Polysaccharides from Ganoderma Lucidum Improve the Histomorphology and Function of Testes in Middle-Aged Male Mice by Alleviating Oxidative Stress and Cellular Apoptosis. Nutrients, 14.","DOI":"10.3390\/nu14224733"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/10408399209527581","article-title":"Phenolic Antioxidants","volume":"32","author":"Shahidi","year":"1992","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.anireprosci.2010.08.006","article-title":"Bacterial Contamination of Ram Semen, Antibiotic Sensitivities, and Effects on Sperm Quality during Storage at 15 \u00b0C","volume":"122","author":"Mateos","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/j.intimp.2004.07.006","article-title":"Isolation and Characterization of Structural Components of Aloe vera L. Leaf Pulp","volume":"4","author":"Ni","year":"2004","journal-title":"Int. Immunopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"22","DOI":"10.5455\/vrn.2022.b9","article-title":"Effect of Aloe vera Gel Extract Treatment on Fertility Rates in Harco Strain Plymouth Rock Male Cockerel","volume":"2","author":"Anifowose","year":"2022","journal-title":"Vet. Res. Notes"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.mrgentox.2009.06.004","article-title":"Aloe-Emodin-Induced DNA Fragmentation in the Mouse in Vivo Comet Assay","volume":"678","author":"Nesslany","year":"2009","journal-title":"Mutat. Res. Toxicol. Environ. Mutagen."},{"key":"ref_32","first-page":"63","article-title":"Effect of Dietary Aloe vera on Growth and Lipid Peroxidation Indices in Rainbow Trout (Oncorhynchus mykiss)","volume":"6","author":"Golestan","year":"2015","journal-title":"Vet. Res. Forum"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"102648","DOI":"10.1016\/j.psj.2023.102648","article-title":"Solid Storage Supplemented with Serine of Rooster Semen Enhances Higher Sperm Quality and Fertility Potential during Storage at 5 \u00b0C for up to 120 h","volume":"102","author":"Kheawkanha","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1093\/humrep\/dey081","article-title":"Creatine Enhances the Duration of Sperm Capacitation: A Novel Factor for Improving in Vitro Fertilization with Small Numbers of Sperm","volume":"33","author":"Umehara","year":"2018","journal-title":"Hum. Reprod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1530\/rep.0.1210267","article-title":"Characterization of Northern Pintail (Anas acuta) Ejaculate and the Effect of Sperm Preservation on Fertility","volume":"121","author":"Penfold","year":"2001","journal-title":"Reprod. Camb. Engl."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3382\/japr.2011-00478","article-title":"Nutritional Limitations during Poultry Embryonic Development1","volume":"21","author":"Uni","year":"2012","journal-title":"J. Appl. Poult. Res."},{"key":"ref_37","unstructured":"Kuda, N.T. (2023). Factors Influencing Fertility and Hatchability of Poultry. J. Biol. Agric. Healthc., 13."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.3382\/ps.0751555","article-title":"Acute High Environmental Temperature and Calcium-Estrogen Relationships in the Hen","volume":"75","author":"Mahmoud","year":"1997","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Pimprasert, M., Kheawkanha, T., Boonkum, W., and Chankitisakul, V. (2023). Influence of Semen Collection Frequency and Seasonal Variations on Fresh and Frozen Semen Quality in Thai Native Roosters. Animals, 13.","DOI":"10.3390\/ani13040573"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103262","DOI":"10.1016\/j.psj.2023.103262","article-title":"Improving the Quality of Chilled Semen from Thai Native Chicken Using Phosphorus and Vitamin B12 Supplementation in Semen Extender","volume":"103","author":"Suwimonteerabutr","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6346","DOI":"10.14233\/ajchem.2013.14552","article-title":"Variation in Amino Acid Content Among Three Aloe Species","volume":"25","author":"Kim","year":"2013","journal-title":"Asian J. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s13197-020-04661-6","article-title":"A Comprehensive Overview of Functional and Rheological Properties of Aloe vera and Its Application in Foods","volume":"58","author":"Sonawane","year":"2021","journal-title":"J. Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Andrea, B., Dumitri\u021ba, R., Florina, C., Francisc, D., Anastasia, V., Socaci, S., and Adela, P. (2020). Comparative Analysis of Some Bioactive Compounds in Leaves of Different Aloe Species. BMC Chem., 14.","DOI":"10.1186\/s13065-020-00720-3"},{"key":"ref_44","first-page":"101","article-title":"In Vitro Antioxidant Activities of Aloe vera Leaf Skin Extracts","volume":"10","author":"Miladi","year":"2008","journal-title":"J. Soci\u00e9t\u00e9 Chim. Tunis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"12732","DOI":"10.15835\/nbha50212732","article-title":"Pot Aloe vera Gel\u2014A Natural Source of Antioxidants","volume":"50","author":"Pop","year":"2022","journal-title":"Not. Bot. Horti Agrobot. Cluj-Napoca"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1235009","DOI":"10.1126\/science.1235009","article-title":"Impact of Shale Gas Development on Regional Water Quality","volume":"340","author":"Vidic","year":"2013","journal-title":"Science"},{"key":"ref_47","first-page":"583","article-title":"The Effect of Dietary L-Carnitine on Semen Quality Parameters and Gonadosomatic and Hepatosomatic Indexes in Broiler Breeder","volume":"14","author":"Mohammadi","year":"2022","journal-title":"Iran. J. Anim. Sci. Res."},{"key":"ref_48","first-page":"76","article-title":"Usefulness of (Aloe vera) as a Potential Ingredient of Cosmetic Preparations","volume":"69","author":"Witaszczyk","year":"2023","journal-title":"Pomeranian J. Life Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/16\/2290\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:31:01Z","timestamp":1760110261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/16\/2290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":48,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["ani14162290"],"URL":"https:\/\/doi.org\/10.3390\/ani14162290","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,6]]}}}