{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:56:12Z","timestamp":1789012572743,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001588","name":"Enterprise Ireland","doi-asserted-by":"publisher","award":["164475"],"award-info":[{"award-number":["164475"]}],"id":[{"id":"10.13039\/501100001588","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The inclusion of rumen buffers in ruminant feeds has gained widespread adoption for the prevention of rumen acidosis, thereby avoiding the negative production and health consequences of low rumen pH and resulting in improved feed efficiency. Benchmarking and quality controlling the performance of rumen buffer materials is of significant interest to feed mills and end-user producers. The aim of this study was to evaluate, develop and optimise a laboratory protocol to consistently and robustly evaluate rumen buffering materials in order to predict their in vivo efficacy. Three different methods were evaluated for determining the buffering potential of carbonate buffer materials: (a) 2 and 8 h static pH, (b) 8 h fixed HCl acid load addition and (c) 3 h acidotic diet simulation using acetic acid. Buffer material, threshold pH, test duration and interactions between all three variables were significant (p &lt; 0.001) in evaluating the performance of the buffer materials. The acidotic diet simulation was found to provide a different ranking of materials to the 8 h fixed HCl acid load methodology. The results highlight the importance of method selection and test parameters for accurately evaluating the potential efficacy of rumen buffer materials.<\/jats:p>","DOI":"10.3390\/ani14162333","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T06:02:37Z","timestamp":1723528957000},"page":"2333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation and Development of Analytical Procedures to Assess Buffering Capacity of Carbonate Ruminant Feed Buffers"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8842-3549","authenticated-orcid":false,"given":"Patrick","family":"Quille","sequence":"first","affiliation":[{"name":"Shannon Applied Biotechnology Centre, Munster Technological University Kerry, Clash, V92CX88 Tralee, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommy","family":"Higgins","sequence":"additional","affiliation":[{"name":"Marigot Researh Centre, Sycamore Court, Clash, V92 N6C8 Tralee, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-7264","authenticated-orcid":false,"given":"Enda W.","family":"Neville","sequence":"additional","affiliation":[{"name":"Celtic Sea Minerals, Strand Farm, Currabinny, P43 NN62 Carrigaline, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katy","family":"Regan","sequence":"additional","affiliation":[{"name":"Celtic Sea Minerals, Strand Farm, Currabinny, P43 NN62 Carrigaline, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"Shannon Applied Biotechnology Centre, Munster Technological University Kerry, Clash, V92CX88 Tralee, Ireland"},{"name":"Marigot Researh Centre, Sycamore Court, Clash, V92 N6C8 Tralee, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tvjl.2007.12.016","article-title":"Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences","volume":"176","author":"Plaizier","year":"2008","journal-title":"Vet. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.anifeedsci.2005.08.004","article-title":"Understanding and preventing subacute ruminal acidosis in dairy herds: A review","volume":"126","author":"Krause","year":"2006","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.anifeedsci.2013.04.017","article-title":"Effect of forage source and a supplementary methionine hydroxyl analogue on rumen fermentation parameters in lactating dairy cows offered a low crude protein diet","volume":"183","author":"Whelan","year":"2013","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_4","first-page":"916","article-title":"Effect of feeding buffer on feed intake, milk production and rumen fermentation pattern in lactating animals: A review","volume":"6","author":"Sharma","year":"2018","journal-title":"J. Entomol. Zool. Stud."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.3168\/jds.S0022-0302(97)76074-0","article-title":"Relationship between fermentation acid production in the rumen and the requirement for physically effective fiber","volume":"80","author":"Allen","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.3168\/jds.S0022-0302(97)76026-0","article-title":"Bovine acidosis: Implications on laminitis","volume":"80","author":"Nocek","year":"1997","journal-title":"J Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.3168\/jds.S0022-0302(05)72807-1","article-title":"Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response","volume":"88","author":"Gozho","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Hobson, P.N., and Stewart, C.S. (1997). Manipulation of ruminal fermentation. The Rumen Microbial Ecosystem, Springer.","DOI":"10.1007\/978-94-009-1453-7"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Millen, D.M., De Beni Arrigoni, M., and Dias Lauritano Pacheco, R. (2016). Ruminal fermentation. Rumenology, Springer.","DOI":"10.1007\/978-3-319-30533-2"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.2527\/1998.7661702x","article-title":"Calculation of the Buffering Capacity of Bicarbonate in the Rumen and In Vitro","volume":"76","author":"Kohn","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3168\/jds.S0022-0302(06)72086-0","article-title":"Effects of physically effective fiber on chewing activity and ruminal pH of dairy cows fed diets based on barley silage","volume":"89","author":"Yang","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.anifeedsci.2011.12.005","article-title":"Ruminal pH regulation and nutritional consequences of low pH","volume":"172","author":"Dijkstra","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"826","DOI":"10.3168\/jds.S0022-0302(93)77407-X","article-title":"Another theory for the action of ruminal buffer salts: Decreased starch fermentation and propionate production","volume":"76","author":"Russell","year":"1993","journal-title":"J. Dairy Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3246","DOI":"10.3168\/jds.S0022-0302(88)79930-0","article-title":"Dietary buffering requirements of the lactating dairy cow: A review","volume":"71","author":"Erdman","year":"1988","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8027","DOI":"10.3168\/jds.2019-16244","article-title":"The effect of calcareous marine algae, with or without marine magnesium oxide, and sodium bicarbonate on rumen pH and milk production in mid-lactation dairy cows","volume":"102","author":"Neville","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.tvjl.2007.12.021","article-title":"The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review","volume":"176","author":"Enemark","year":"2008","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.3168\/jds.S0022-0302(89)79299-7","article-title":"Effect of Type and Amount of Buffer Addition to Grass Silage-Based Total Mixed Rations on Milk Production and Composition","volume":"72","author":"Harrison","year":"1989","journal-title":"J. Dairy Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1017\/S1751731119000296","article-title":"A comparative study of four rumen buffering agents on productive performance, rumen fermentation and meat quality in growing lambs fed a total mixed ration","volume":"13","author":"Alhidary","year":"2019","journal-title":"Animal"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ramos, S.C., Kim, S.H., Jeong, C.D., Mamuad, L.L., Son, A.R., Kang, S.H., Cho, Y.I., Kim, T.G., Lee, J.S., and Cho, K.K. (2022). Increasing buffering capacity enhances rumen fermentation characteristics and alters rumen microbiota composition of high-concentrate fed Hanwoo steers. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-24777-3"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5506","DOI":"10.3168\/jds.2014-8875","article-title":"The effect of buffering dairy cow diets with limestone, calcareous marine algae, or sodium bicarbonate on ruminal pH profiles, production responses, and rumen fermentation","volume":"98","author":"Cruywagen","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0377-8401(95)00869-1","article-title":"Animal nutrition and management in the 21st century: Dairy cattle","volume":"58","author":"Chalupa","year":"1996","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9777","DOI":"10.3168\/jds.2017-14353","article-title":"Modulation of rumen pH by sodium bicarbonate and a blend of different sources of magnesium oxide in lactating dairy cows sub- mitted to a concentrate challenge","volume":"101","author":"Bach","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"712","DOI":"10.3168\/jds.S0022-0302(82)82259-5","article-title":"Dietary sodium bicarbonate and magnesium oxide for early postpartum lactating dairy cows: Effects of production, acid-based metabolism, and digestion","volume":"65","author":"Erdman","year":"1982","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Van Soest, P.J. (1994). Nutritional Ecology of the Ruminant, Cornell University Press. [2nd ed.]. Available online: https:\/\/www.jstor.org\/stable\/10.7591\/j.ctv5rf668.","DOI":"10.7591\/9781501732355"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"114255","DOI":"10.1016\/j.anifeedsci.2019.114255","article-title":"Feeding a marine-based rumen buffer increases milk production and decreases time of low reticulo-rumen pH in grazing dairy cows offered perennial ryegrass-based pasture","volume":"256","author":"Rafferty","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.3168\/jds.S0022-0302(87)80170-4","article-title":"Use of Acid and Base Titrations to Evaluate the Buffering Capacity of Ruminant Feedstuffs In Vitro","volume":"70","author":"Wohlt","year":"1987","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"72","DOI":"10.3844\/ajavsp.2007.72.78","article-title":"The Determination of Buffering Capacity of Some Ruminant\u2019s Feedstuffs and their Cumulative Effects on TMR Ration","volume":"2","author":"Moharrery","year":"2007","journal-title":"Am. J. Anim. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2046-0481-58-8-447","article-title":"Measurements of the acid-binding capacity of ingredients used in pig diets","volume":"58","author":"Lawlor","year":"2005","journal-title":"Ir. Vet. J."},{"key":"ref_29","unstructured":"Zaba, A. (2024, July 02). Finding the Area under a Curve Using Riemann Sums, the Trapezoidal Rule, and Integration. Available online: https:\/\/www.researchgate.net\/publication\/319186191_Finding_the_area_under_a_curve_using_Riemann_sums_the_trapezoidal_rule_and_integration?channel=doi&linkId=599a10e6a6fdcc261586b152&showFulltext=true."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Nash, M.C., Diaz-Pulido, G., Harvey, A.S., and Adey, W. (2019). Coralline algal calcification: A morphological and process-based understanding. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0221396"},{"key":"ref_31","unstructured":"OBIS Ocean Biodiversity Information System (2024, June 26). Intergovernmental Oceanographic Commission of UNESCO. Available online: https:\/\/obis.org\/."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.gca.2007.11.019","article-title":"Coralline algae are global palaeothermometers with bi-weekly resolution","volume":"72","author":"Kamenos","year":"2008","journal-title":"Geochim. Cosmochim Acta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3920\/JAAN2021.0015","article-title":"Global survey of limestone used in poultry diets: Calcium content, particle size and solubility","volume":"10","author":"Saad","year":"2022","journal-title":"J. Appl. Anim. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"811","DOI":"10.3168\/jds.S0022-0302(92)77820-5","article-title":"A buffer value index to evaluate effects of buffers on ruminal milieu in cows fed high or low concentrate, silage, or hay diets","volume":"75","author":"Tucker","year":"1992","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1093\/japr\/6.1.94","article-title":"Coon. Improved in vitro Methods for Determining Limestone and Oyster Shell Solubility","volume":"6","author":"Zhang","year":"1997","journal-title":"J. Appl. Poult. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6837","DOI":"10.3382\/ps\/pez423","article-title":"Modification of a limestone solubility method and potential to correlate with in vivo limestone calcium digestibility","volume":"98","author":"Kim","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.3168\/jds.S0022-0302(98)75886-2","article-title":"The importance of pH in the regulation of ruminal acetate to propionate ratio and methane production in vitro","volume":"81","author":"Russell","year":"1998","journal-title":"J. Dairy Sci."},{"key":"ref_38","unstructured":"Nehrke, G. (2007). Calcite Precipitation from Aqueous solution: Transformation from Vaterite and Role of Solution Stoichiometry. [Ph.D. Thesis, Universit\u00e4t Utrecht]."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1017\/S1751731115002888","article-title":"Relative contribution of ruminal buffering systems to pH regulation in feedlot cattle fed either low- or high-forage diets","volume":"10","author":"Chibisa","year":"2016","journal-title":"Animal"},{"key":"ref_40","unstructured":"(2024, June 25). Bordwell pKa Table. Available online: https:\/\/organicchemistrydata.org\/hansreich\/resources\/pka\/#kainorgacid."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.anifeedsci.2016.06.026","article-title":"The role of dissolved carbon dioxide in both the decline in rumen pH and nutritional diseases in ruminants","volume":"219","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.3168\/jds.S0022-0302(92)77851-5","article-title":"Ruminal Buffers: Temporal Effects on Buffering Capacity and pH of Ruminal Fluid from Cows Fed a High Concentrate Diet","volume":"75","author":"Tucker","year":"1992","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/16\/2333\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:35:44Z","timestamp":1760110544000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/16\/2333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,13]]},"references-count":42,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["ani14162333"],"URL":"https:\/\/doi.org\/10.3390\/ani14162333","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,13]]}}}