{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:46:59Z","timestamp":1791974819522,"version":"4.4.0"},"reference-count":91,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T00:00:00Z","timestamp":1723939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"North Carolina Agricultural Foundation","award":["#660101"],"award-info":[{"award-number":["#660101"]}]},{"name":"North Carolina Agricultural Foundation","award":["#662825"],"award-info":[{"award-number":["#662825"]}]},{"name":"North Carolina Agricultural Foundation","award":["#02893"],"award-info":[{"award-number":["#02893"]}]},{"name":"USDA-NIFA Hatch","award":["#660101"],"award-info":[{"award-number":["#660101"]}]},{"name":"USDA-NIFA Hatch","award":["#662825"],"award-info":[{"award-number":["#662825"]}]},{"name":"USDA-NIFA Hatch","award":["#02893"],"award-info":[{"award-number":["#02893"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objectives of this review are to investigate how benzoic acid can mitigate the negative effects of weaning stress, improve the intestinal microbiota, intestinal health, and growth of nursery pigs, determine the optimal dose level of benzoic acid for the growth rate in nursery pigs, and compare the efficacy of benzoic acid and other acids in pig feeds. After weaning, pigs are exposed to less lactose and solid feed with high acid-binding capacity at infrequent intervals, causing an increase in digesta pH, reducing protein digestion, and increasing ammonia-producing bacteria in the stomach. Benzoic acid supplementation has improved the intestinal health and growth of nursery pigs through its antimicrobial properties and pH reduction in the digesta. The positive modulation of luminal microbiota in the small intestine of pigs by benzoic acid improves intestinal morphology and enhances nutrient utilization, especially nitrogen, of nursery pigs. Benzoic acid supplementation of up to 1% in feeds also increases hippuric acid contents in the urine of nursery pigs, decreasing urinary pH, which is related to ammonia emission and barn conditions in intensive pig production. Supported by the beneficial impacts of benzoic acid, the growth performance of nursery pigs was also improved. However, excessive benzoic acid (over 2.5% up to 5%) in feeds reduces the growth performance of nursery pigs. Thus, this review conducted a meta-analysis of the results from 16 papers to determine the optimal dose level of benzoic acid for body weight gain of nursery pigs, which was found to be 0.60%. The efficacy of benzoic acid was similar to that of other organic acids, including citric acid, fumaric acid, formic acid, and formate salts. Collectively, benzoic acid supplementation can positively modulate the luminal and mucosal microbiota in the small intestine, increase nutrient utilization and intestinal health, decrease urinary pH, and improve the growth performance of nursery pigs.<\/jats:p>","DOI":"10.3390\/ani14162394","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T05:19:36Z","timestamp":1724044776000},"page":"2394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dietary Intervention of Benzoic Acid for Intestinal Health and Growth of Nursery Pigs"],"prefix":"10.3390","volume":"14","author":[{"given":"Hyunjun","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4591-1943","authenticated-orcid":false,"given":"Sung Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Campbell, J.M., Crenshaw, J.D., and Polo, J. 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