{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T09:50:26Z","timestamp":1786787426071,"version":"build-2736575974"},"reference-count":29,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AniCura\u2019s Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Recently, human epidermal growth factor receptor 2 (HER2) has emerged as a therapeutic target of interest for non-small-cell lung cancer in humans. The role of HER2 in canine pulmonary adenocarcinomas is poorly documented. To address this gap, this study employed three methodologies: immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS) to investigate the protein expression, gene amplification, and mutation of HER2 in 19 canine primary pulmonary adenocarcinomas. By IHC, 3 out of 19 cases were overexpressed 3+, 6 were 2+, and 10 were negative. With FISH, 2 cases were amplified (12.5%), 3 were inadequate for the analyses, and the others were non-amplified. With NGS, seven cases were inadequate. All other cases were wild-type, except for one IHC 3+ case, which was amplified with FISH and with a specific mutation already described in human pulmonary adenocarcinoma, V659E. This mutation is probably sensitive to tyrosine kinase inhibitory drugs. These results are similar to those in human medicine and to the few data in the literature on canine lung carcinomas; the presence of 12.5% of amplified cases in dogs lays the foundation for future targeted drugs against HER2 alterations.<\/jats:p>","DOI":"10.3390\/ani14182625","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T03:16:51Z","timestamp":1725938211000},"page":"2625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protein Expression, Amplification, and Mutation of HER2 Gene in Canine Primary Pulmonary Adenocarcinomas: Preliminary Results"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2694-905X","authenticated-orcid":false,"given":"Barbara","family":"Brunetti","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0609-8817","authenticated-orcid":false,"given":"Dario","family":"de Biase","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostics and Specialty Medicine, University of Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5661-1538","authenticated-orcid":false,"given":"Francesca","family":"Millanta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-0624","authenticated-orcid":false,"given":"Luisa Vera","family":"Muscatello","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Di Oto","sequence":"additional","affiliation":[{"name":"OaCP IE, Ltd., T12H1XY Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester Lidia","family":"Laddaga","sequence":"additional","affiliation":[{"name":"Ospedale Veterinario \u201cI Portoni Rossi\u201d, Anicura, Zola Predosa, 40069 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3761-5135","authenticated-orcid":false,"given":"Antonio","family":"De Leo","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostics and Specialty Medicine, University of Bologna, 40126 Bologna, Italy"},{"name":"Solid Tumor Molecular Pathology Laboratory, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0113-6682","authenticated-orcid":false,"given":"Giovanni","family":"Tallini","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostics and Specialty Medicine, University of Bologna, 40126 Bologna, Italy"},{"name":"Solid Tumor Molecular Pathology Laboratory, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Bacci","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1093\/emboj\/17.12.3385","article-title":"Differential Endocytic Routing of Homo- and Hetero-Dimeric ErbB Tyrosine Kinases Confers Signaling Superiority to Receptor Heterodimers","volume":"17","author":"Lenferink","year":"1998","journal-title":"EMBO J."},{"key":"ref_2","first-page":"122","article-title":"HER2 Driven Non-Small Cell Lung Cancer (NSCLC): Potential Therapeutic Approaches","volume":"2","author":"Felip","year":"2013","journal-title":"Transl. 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