{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T21:07:54Z","timestamp":1788210474737,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanxi Province Excellent Doctoral Work Award-Scientific Research Project","award":["SXBYKY2021019"],"award-info":[{"award-number":["SXBYKY2021019"]}]},{"name":"Shanxi Province Excellent Doctoral Work Award-Scientific Research Project","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"Shanxi Province Excellent Doctoral Work Award-Scientific Research Project","award":["20211331-13"],"award-info":[{"award-number":["20211331-13"]}]},{"name":"Shanxi Province Excellent Doctoral Work Award-Scientific Research Project","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["SXBYKY2021019"],"award-info":[{"award-number":["SXBYKY2021019"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["20211331-13"],"award-info":[{"award-number":["20211331-13"]}]},{"name":"Research Fund of Shanxi Province for Introduced High-level Leading Talents","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]},{"name":"Shanxi \u201c1331 Project\u201d","award":["SXBYKY2021019"],"award-info":[{"award-number":["SXBYKY2021019"]}]},{"name":"Shanxi \u201c1331 Project\u201d","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"Shanxi \u201c1331 Project\u201d","award":["20211331-13"],"award-info":[{"award-number":["20211331-13"]}]},{"name":"Shanxi \u201c1331 Project\u201d","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]},{"name":"pecial Research Fund of Shanxi Agricultural University for High-level Talents","award":["SXBYKY2021019"],"award-info":[{"award-number":["SXBYKY2021019"]}]},{"name":"pecial Research Fund of Shanxi Agricultural University for High-level Talents","award":["RFSXIHLT202101"],"award-info":[{"award-number":["RFSXIHLT202101"]}]},{"name":"pecial Research Fund of Shanxi Agricultural University for High-level Talents","award":["20211331-13"],"award-info":[{"award-number":["20211331-13"]}]},{"name":"pecial Research Fund of Shanxi Agricultural University for High-level Talents","award":["2021XG001"],"award-info":[{"award-number":["2021XG001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Two species of trichomonads, Tetratrichomonas buttreyi and Pentatrichomonas hominis, are common intestinal parasites that can impact animal health and productivity. Severe infection by these parasites can lead to diarrhea and wasting in affected animals. Notably, P. hominis is known to cause diarrhea and has the potential to be transmitted between animals and humans. Donkeys hold significant economic importance in China\u2019s agricultural sector. However, whether donkeys are infected with T. buttreyi and P. hominis remains unknown globally. To address this gap in knowledge, 815 fecal samples were collected from donkeys in three representative regions in Shanxi Province, North China. Then, the presence and genetic characteristics of T. buttreyi and P. hominis were examined using species-specific PCR primers amplifying the small subunit ribosomal RNA genes. The overall prevalence was detected to be 25.4% (207\/815) for T. buttreyi and 0.7% (6\/815) for P. hominis in donkeys in Shanxi Province. All obtained P. hominis sequences were identified as genotype CC1. Genetic analysis revealed that all P. hominis isolates from donkeys were clustered into the same branch with isolates detected in humans, suggesting possible zoonotic transmission. This study is the first to report the occurrence and prevalence of T. buttreyi and P. hominis in donkeys globally. These findings expand the host range of trichomonads and improve our understanding of their genetic diversity and zoonotic potential, providing essential baseline data for the prevention and control of these parasites in donkeys in the region.<\/jats:p>","DOI":"10.3390\/ani14182651","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T05:03:04Z","timestamp":1726117384000},"page":"2651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["First Molecular Detection and Genetic Characterization of Tetratrichomonas buttreyi and Pentatrichomonas hominis in Donkeys in Shanxi Province, China"],"prefix":"10.3390","volume":"14","author":[{"given":"Han-Dan","family":"Xiao","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Han","family":"Lv","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze-Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Su","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Liang","family":"Li","sequence":"additional","affiliation":[{"name":"Taiyuan Zoo, Taiyuan 030009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":false,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4512-4632","authenticated-orcid":false,"given":"Shi-Chen","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Wei","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1177\/1098612X17693499","article-title":"The conundrum of feline trichomonosis","volume":"19","author":"Gookin","year":"2017","journal-title":"J. Feline Med. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2010.03.022","article-title":"Design and validation of an oligonucleotide probe for the detection of protozoa from the order trichomonadida using chromogenic in situ hybridization","volume":"171","author":"Mostegl","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_3","first-page":"2055116918774959","article-title":"Pentatrichomonas hominis infection in two domestic cats with chronic diarrhea","volume":"4","author":"Bastos","year":"2018","journal-title":"JFMS Open. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0304-4017(96)01140-5","article-title":"Isolation of the trichomonad Tetratrichomonas buttreyi (Hibler et al., 1960) Honigberg, 1963 in bovine diarrhoeic faeces","volume":"70","author":"Castella","year":"1997","journal-title":"Vet. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1007\/s00436-008-1134-x","article-title":"Ultrastructural study of a tetratrichomonad isolated from pig fecal samples","volume":"103","author":"Rivera","year":"2008","journal-title":"Parasitol. Res."},{"key":"ref_6","first-page":"602","article-title":"Survey and molecular characterization of trichomonads in pigs in Anhui Province, East China, 2014","volume":"13","author":"Li","year":"2018","journal-title":"Iran. J. Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2010.12.022","article-title":"Investigations on the prevalence and potential pathogenicity of intestinal trichomonads in pigs using in situ hybridization","volume":"178","author":"Mostegl","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.vetpar.2015.04.028","article-title":"The prevalence of intestinal trichomonads in Chinese pigs","volume":"211","author":"Li","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.vetpar.2007.09.007","article-title":"Tetratrichomonas spp. and Pentatrichomonas hominis are not persistently detectable after intravaginal inoculation of estrous heifers","volume":"150","author":"Cobo","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1136\/jcp.2011.089326","article-title":"Molecular identification of Pentatrichomonas hominis in two patients with gastrointestinal symptoms","volume":"64","author":"Meloni","year":"2011","journal-title":"J. Clin. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1538\/expanim1978.39.2_187","article-title":"Pentatrichomonas hominis from beagle dogs\u2014Detection method, characteristics and route of infection","volume":"39","author":"Fukushima","year":"1990","journal-title":"Exp. Anim."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetpar.2006.10.020","article-title":"Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay","volume":"145","author":"Gookin","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1007\/s12639-022-01506-1","article-title":"Pentatrichomonas hominis and other intestinal parasites in school-aged children: Coproscopic survey","volume":"46","author":"Abdo","year":"2022","journal-title":"J. Parasit. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhang, H., Yu, Y., Li, J., Gong, P., Wang, X., Li, X., Cheng, Y., Yu, X., Zhang, N., and Zhang, X. (2022). Changes of gut microbiota in colorectal cancer patients with Pentatrichomonas hominis infection. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.961974"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s00436-023-08099-5","article-title":"Prevalence of Pentatrichomonas hominis in foxes and raccoon dogs and changes in the gut microbiota of infected female foxes in the Hebei and Henan Provinces in China","volume":"123","author":"Song","year":"2023","journal-title":"Parasitol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1186\/s13071-019-3684-4","article-title":"High prevalence of Pentatrichomonas hominis infection in gastrointestinal cancer patients","volume":"12","author":"Zhang","year":"2019","journal-title":"Parasit. Vectors"},{"key":"ref_17","first-page":"119","article-title":"Intestinal parasitic infections and anaemia among pregnant women in the highlands of Papua New Guinea","volume":"56","author":"Phuanukoonnon","year":"2013","journal-title":"Papua New Guin. Med. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.pt.2014.05.005","article-title":"What is the importance of zoonotic trichomonads for human health?","volume":"30","author":"Maritz","year":"2014","journal-title":"Trends Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1215.e1","DOI":"10.1016\/j.ajem.2019.03.029","article-title":"Tetratrichomonas in pyopneumothorax","volume":"37","author":"Dong","year":"2019","journal-title":"Am. J. Emerg. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.vetpar.2003.07.026","article-title":"Ultrastructural study of a tetratrichomonad species isolated from prepucial smegma of virgin bulls","volume":"117","author":"Cobo","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/s13071-023-05756-7","article-title":"Digital PCR: Modern solution to parasite diagnostics and population trait genetics","volume":"16","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev.food.080708.100730","article-title":"Rapid detection and limitations of molecular techniques","volume":"2","author":"Maurer","year":"2011","journal-title":"Annu. Rev. Food. Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1111\/j.1939-1676.2006.tb02893.x","article-title":"Efficacy of ronidazole for treatment of feline Tritrichomonas foetus infection","volume":"20","author":"Gookin","year":"2006","journal-title":"J. Vet. Intern. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.cveq.2019.08.005","article-title":"Donkey and mule welfare","volume":"35","author":"Davis","year":"2019","journal-title":"Vet. Clin. N. Am. Equine. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"103642","DOI":"10.1016\/j.jevs.2021.103642","article-title":"Donkey industry in China: Current aspects, suggestions and future challenges","volume":"102","author":"Seyiti","year":"2021","journal-title":"J. Equine Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wang, X., Wang, T., Liang, H., Wang, L., Akhtar, F., Shi, X., Ren, W., Huang, B., Kou, X., and Chen, Y. (2023). A novel SNP in NKX1-2 gene is associated with carcass traits in Dezhou donkey. BMC Genom. Data, 24.","DOI":"10.1186\/s12863-022-01101-6"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Yu, M., Zhang, X., Yan, J., Guo, J., Zhang, F., Zhu, K., Liu, S., Sun, Y., Shen, W., and Wang, J. (2022). Transcriptional specificity analysis of testis and epididymis tissues in Donkey. Genes, 13.","DOI":"10.3390\/genes13122339"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1111\/1749-4877.12629","article-title":"Prevalence and molecular characterization of Pentatrichomonas hominis in Siberian tigers (Panthera tigris altaica) in northeast China","volume":"17","author":"Zhang","year":"2022","journal-title":"Integr. Zool."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1017\/S003118202100086X","article-title":"Sporadic isolation of tetratrichomonas species from the cattle urogenital tract","volume":"148","author":"Bailey","year":"2021","journal-title":"Parasitology"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mahittikorn, A., Udonsom, R., Koompapong, K., Chiabchalard, R., Sutthikornchai, C., Sreepian, P.M., Mori, H., and Popruk, S. (2021). Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-02904-y"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s00436-019-06550-0","article-title":"Prevalence of Tetratrichomonas buttreyi and Pentatrichomonas hominis in yellow cattle, dairy cattle, and water buffalo in China","volume":"119","author":"Li","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.vetpar.2013.03.019","article-title":"Molecular characterization of trichomonads isolated from animal hosts in the Philippines","volume":"196","author":"Dimasuay","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wang, Z.R., Fan, Q.X., Wang, J.L., Zhang, S., Wang, Y.X., Zhang, Z.D., Gao, W.W., Zhu, X.Q., and Liu, Q. (2024). Molecular identi-fication and survey of Trichomonad species in pigs in Shanxi Province, North China. Vet. Sci., 11.","DOI":"10.3390\/vetsci11050203"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gao, W.W., Zhang, S., Zhang, T.H., Xiao, H.D., Su, N., Tao, M.F., Wu, Z.X., Zhang, Z.D., Zhu, X.Q., and Xie, S.C. (2023). Prevalence and multilocus genotyping of Giardia duodenalis in donkeys in Shanxi Province, North China. Animals, 13.","DOI":"10.3390\/ani13243771"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1051\/parasite\/2024018","article-title":"Prevalence of in-testinal trichomonads in captive non-human primates in China","volume":"31","author":"Ma","year":"2024","journal-title":"Parasite"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1007\/s00436-015-4773-8","article-title":"Pentatrichomonas hominis: Prevalence and molecular characterization in humans, dogs, and monkeys in Northern China","volume":"115","author":"Li","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Peachey, L.E., Castro, C., Molena, R.A., Jenkins, T.P., Griffin, J.L., and Cantacessi, C. (2019). Dysbiosis associated with acute helminth infections in herbivorous youngstock - observations and implications. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-47204-6"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1042\/BCJ20160510","article-title":"Introduction to the human gut microbiota","volume":"474","author":"Thursby","year":"2017","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Dheilly, N.M., Ewald, P.W., Brindley, P.J., Fichorova, R.N., and Thomas, F. (2019). Parasite-microbe-host interactions and cancer risk. PLoS Pathog., 15.","DOI":"10.1371\/journal.ppat.1007912"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13071-018-2671-5","article-title":"Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China","volume":"11","author":"Li","year":"2018","journal-title":"Parasit. Vectors"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1007\/s00436-014-3825-9","article-title":"Pentatrichomonas hominis: First isolation from the feces of a dog with diarrhea in China","volume":"113","author":"Li","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"109851","DOI":"10.1016\/j.vetpar.2022.109851","article-title":"Molecular diagnostics for gastrointestinal helminths in equids: Past, present and future","volume":"313","author":"Ghafar","year":"2023","journal-title":"Vet. Parasitol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2016.11.012","article-title":"Leishmania infections: Molecular targets and diagnosis","volume":"57","author":"Akhoundi","year":"2017","journal-title":"Mol. Aspects Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/18\/2651\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:54:28Z","timestamp":1760111668000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/18\/2651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":43,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ani14182651"],"URL":"https:\/\/doi.org\/10.3390\/ani14182651","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,12]]}}}