{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T08:57:26Z","timestamp":1776502646190,"version":"3.51.2"},"reference-count":61,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T00:00:00Z","timestamp":1727049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Startup Foundation of Zhejiang Agriculture and Forestry University","award":["W20210011"],"award-info":[{"award-number":["W20210011"]}]},{"name":"Research Startup Foundation of Zhejiang Agriculture and Forestry University","award":["2023SNJF057"],"award-info":[{"award-number":["2023SNJF057"]}]},{"name":"\u201cThree Rural Nine Party\u201d Program of Zhejiang Province","award":["W20210011"],"award-info":[{"award-number":["W20210011"]}]},{"name":"\u201cThree Rural Nine Party\u201d Program of Zhejiang Province","award":["2023SNJF057"],"award-info":[{"award-number":["2023SNJF057"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Photoperiod can regulate the broodiness of geese and thus increase their egg-laying rate. The laying performance of geese is mainly determined by ovary and follicle development. To understand the effect of photoperiod on the ovary and small white follicles, sixteen 220-day-old healthy female Zhedong white geese were randomly divided into two groups for long photoperiods (15L:9D) and short photoperiods (9L:15D). The geese were euthanized after two months of feeding, and their ovaries and follicles were collected for transcriptome sequencing. RNA-seq analysis identified 187 and 448 differentially expressed genes in ovaries and small white follicles of different photoperiod groups, respectively. A long photoperiod promotes high expression of SPP1, C6, MZB1, GP1BA, and FCGBP genes in the ovaries, and increases the expression of SPP1, ANGPTL5, ALPL, ZP1, and CHRNA4 genes in small white follicles. Functional enrichment analysis showed that photoperiod could affect respiratory system development, smooth muscle cell proliferation in ovaries, and extracellular matrix-related function in small white follicles. WGCNA revealed 31 gene modules, of which 2 were significantly associated with ovarian weight and 17 with the number of small white follicles. Our results provide a better understanding of the molecular regulation in the photoperiod affecting goose reproduction.<\/jats:p>","DOI":"10.3390\/ani14182747","type":"journal-article","created":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T09:15:07Z","timestamp":1727082907000},"page":"2747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Different Photoperiods on the Transcriptome of the Ovary and Small White Follicles in Zhedong White Geese"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3809-1975","authenticated-orcid":false,"given":"Tao","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meina","family":"Fei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6446-9439","authenticated-orcid":false,"given":"Ke","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songbai","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8789-7858","authenticated-orcid":false,"given":"Ayong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bao, Q., Gu, W., Song, L., Weng, K., Cao, Z., Zhang, Y., Zhang, Y., Ji, T., Xu, Q., and Chen, G. (2023). The Photoperiod-Driven Cyclical Secretion of Pineal Melatonin Regulates Seasonal Reproduction in Geese (Anser cygnoides). Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241511998"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1080\/00071660412331286262","article-title":"Constant photoperiods and sexual maturity in broiler breeder pullets","volume":"45","author":"Lewis","year":"2004","journal-title":"Br. Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.3382\/ps.2011-01652","article-title":"Influence of red light on reproductive performance, eggshell ultrastructure, and eye morphology in Thai-native hens","volume":"90","author":"Gongruttananun","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.3382\/ps.2013-03799","article-title":"Red light is necessary to activate the reproductive axis in chickens independently of the retina of the eye","volume":"93","author":"Baxter","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1093\/ps\/80.8.1112","article-title":"Effects of light intensity from photostimulation in four strains of commercial egg layers: 1. Ovarian morphology and carcass parameters","volume":"80","author":"Renema","year":"2001","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"897","DOI":"10.3382\/ps.0720897","article-title":"Photoperiodic control of reproduction in the domestic hen","volume":"72","author":"Sharp","year":"1993","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.anireprosci.2008.05.073","article-title":"Supplementary artificial light to increase egg production of geese under natural lighting conditions","volume":"113","author":"Wang","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.anireprosci.2019.02.003","article-title":"Analyses of mathematical models for Yangzhou geese egg-laying curves","volume":"203","author":"Guo","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8984","DOI":"10.1002\/jcp.26844","article-title":"Promotion of the prehierarchical follicle growth by postovulatory follicles involving PGE(2) -EP2 signaling in chickens","volume":"233","author":"Lin","year":"2018","journal-title":"J. Cell Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1439-0531.2012.02087.x","article-title":"Follicle selection in the avian ovary","volume":"47","author":"Johnson","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3382\/ps\/peu008","article-title":"Ovarian follicle selection and granulosa cell differentiation","volume":"94","author":"Johnson","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1093\/ps\/80.1.47","article-title":"Effects of photostimulatory light intensity on ovarian morphology and carcass traits at sexual maturity in modern and antique egg-type pullets","volume":"80","author":"Renema","year":"2001","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.ygcen.2013.09.007","article-title":"Increased hepatic yolk precursor synthesis, secretion and facilitated uptake by follicles are involved in the rejuvenation of reproductive performance of molted hens (Gallus gallus domesticus)","volume":"194","author":"Meng","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/00071668.2014.989489","article-title":"Supplementary dietary nitric oxide donor (sodium nitroprusside) or inhibitor (NG-nitro-L-arginine methyl ester) depressed growth performance and ovarian primordial and primary follicles in Japanese quail (Coturnix coturnix japonica) in a dose-dependent manner","volume":"56","author":"Bulbul","year":"2015","journal-title":"Br. Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"894","DOI":"10.3382\/ps\/pew316","article-title":"Effects of dietary octacosanol supplementation on laying performance, egg quality, serum hormone levels, and expression of genes related to the reproductive axis in laying hens","volume":"96","author":"Long","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nrg2484","article-title":"RNA-Seq: A revolutionary tool for transcriptomics","volume":"10","author":"Wang","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1016\/j.psj.2020.10.049","article-title":"Seasonal differences in the transcriptome profile of the Zhedong white goose (Anser cygnoides) pituitary gland","volume":"100","author":"Zhao","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1080\/00071668.2018.1459474","article-title":"Transcriptome profiling to identify key mediators of granulosa cell proliferation upon FSH stimulation in the goose (Anser cygnoides)","volume":"59","author":"Du","year":"2018","journal-title":"Br. Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1111\/jpn.13494","article-title":"Characterization of ovarian morphology and reproductive hormones in Zhedong white geese (Anser cygnoides domesticus) during the reproductive cycle","volume":"105","author":"Wang","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: An R package for identifying differentially expressed genes from RNA-seq data","volume":"26","author":"Wang","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein-protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic. Acids. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: An R package for weighted correlation network analysis. BMC Bioinform., 9.","DOI":"10.1186\/1471-2105-9-559"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1095\/biolreprod44.2.305","article-title":"Stage of ovarian follicular development associated with the initiation of steroidogenic competence in avian granulosa cells","volume":"44","author":"Tilly","year":"1991","journal-title":"Biol. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"E973","DOI":"10.1152\/ajpendo.00104.2003","article-title":"BMPs and BMPRs in chicken ovary and effects of BMP-4 and -7 on granulosa cell proliferation and progesterone production in vitro","volume":"285","author":"Onagbesan","year":"2003","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"25628","DOI":"10.1074\/jbc.273.40.25628","article-title":"Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2","volume":"273","author":"Hoodless","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/jcp.1080","article-title":"Divergence and convergence of TGF-beta\/BMP signaling","volume":"187","author":"Miyazono","year":"2001","journal-title":"J. Cell Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1095\/biolreprod67.2.473","article-title":"Bone morphogenetic protein receptor type II is a receptor for growth differentiation factor-9","volume":"67","author":"Vitt","year":"2002","journal-title":"Biol. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1210\/endo-117-6-2313","article-title":"Somatomedin-C-mediated potentiation of follicle-stimulating hormone-induced aromatase activity of cultured rat granulosa cells","volume":"117","author":"Adashi","year":"1985","journal-title":"Endocrinology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1172\/JCI116028","article-title":"Granulosa cell-derived insulin-like growth factor (IGF) binding proteins are inhibitory to IGF-I hormonal action. Evidence derived from the use of a truncated IGF-I analogue","volume":"90","author":"Adashi","year":"1992","journal-title":"J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1530\/jrf.0.1040259","article-title":"Effects of insulin-like growth factor I and interactions with transforming growth factor alpha and LH on proliferation of chicken granulosa cells and production of progesterone in culture","volume":"104","author":"Onagbesan","year":"1995","journal-title":"J. Reprod. Fertil."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0739-7240(99)00046-6","article-title":"Insulin-like growth factors in the regulation of avian ovarian functions","volume":"17","author":"Onagbesan","year":"1999","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.domaniend.2007.03.001","article-title":"IGF-1 receptor signaling pathways and effects of AMPK activation on IGF-1-induced progesterone secretion in hen granulosa cells","volume":"34","author":"Tosca","year":"2008","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.cbpa.2016.06.035","article-title":"Regulation of sex steroid production and mRNAs encoding gonadotropin receptors and steroidogenic proteins by gonadotropins, cyclic AMP and insulin-like growth factor-I in ovarian follicles of rainbow trout (Oncorhynchus mykiss) at two stages of vitellogenesis","volume":"201","author":"Nakamura","year":"2016","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1530\/jrf.0.1160073","article-title":"Differential effects of amount of feeding on cell proliferation and progesterone production in response to gonadotrophins and insulin-like growth factor I by ovarian granulosa cells of broiler breeder chickens selected for fatness or leanness","volume":"116","author":"Onagbesan","year":"1999","journal-title":"J. Reprod. Fertil."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1530\/reprod\/120.2.433","article-title":"Effect of TNF-alpha on LH and IGF-I modulated chicken granulosa cell proliferation and progesterone production during follicular development","volume":"120","author":"Onagbesan","year":"2000","journal-title":"J. Reprod. Fertil."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1530\/rep.0.1230291","article-title":"Modulatory effects of gonadotrophins and insulin-like growth factor on the secretion of inhibin A and progesterone by granulosa cells from chicken preovulatory (F1-F3) follicles","volume":"123","author":"Lovell","year":"2002","journal-title":"Reproduction"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/0739-7240(91)90028-I","article-title":"Immunolocalization of steroidogenic cells in small follicles of the chicken ovary: Anatomical arrangement and location of steroidogenic cells change during follicular development","volume":"8","author":"Nitta","year":"1991","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1095\/biolreprod29.4.953","article-title":"Steroid metabolism by avian ovarian cells during follicular maturation","volume":"29","author":"Marrone","year":"1983","journal-title":"Biol. Reprod."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1016\/j.psj.2019.12.067","article-title":"Transcriptome profiling analysis of underlying regulation of growing follicle development in the chicken","volume":"99","author":"Zhou","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/bs.vh.2018.01.013","article-title":"The effect of steroid hormones on ovarian follicle development","volume":"107","author":"Chou","year":"2018","journal-title":"Vitam. Horm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/rda.13111","article-title":"Comparative analysis of ovarian transcriptomes between prolific and non-prolific goat breeds via high-throughput sequencing","volume":"53","author":"Zi","year":"2018","journal-title":"Reprod. Domest. Anim."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"18743","DOI":"10.4238\/2015.December.28.23","article-title":"Expression profiles of differentially expressed genes affecting fecundity in goat ovarian tissues","volume":"14","author":"Ling","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chen, P.R., Shin, S., Choi, Y.M., Kim, E., Han, J.Y., and Lee, K. (2016). Overexpression of G0\/G1 Switch Gene 2 in Adipose Tissue of Transgenic Quail Inhibits Lipolysis Associated with Egg Laying. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030384"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11745-012-3742-6","article-title":"Loss of fat with increased adipose triglyceride lipase-mediated lipolysis in adipose tissue during laying stages in quail","volume":"48","author":"Yang","year":"2013","journal-title":"Lipids"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s11745-013-3756-8","article-title":"Porcine G(0)\/G(1) switch gene 2 (G0S2) expression is regulated during adipogenesis and short-term in-vivo nutritional interventions","volume":"48","author":"Ahn","year":"2013","journal-title":"Lipids"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.4161\/cc.9.14.12181","article-title":"Differential control of ATGL-mediated lipid droplet degradation by CGI-58 and G0S2","volume":"9","author":"Lu","year":"2010","journal-title":"Cell Cycle"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"367","DOI":"10.2527\/jas.2010-3339","article-title":"Cloning of avian G(0)\/G(1) switch gene 2 genes and developmental and nutritional regulation of G(0)\/G(1) switch gene 2 in chicken adipose tissue","volume":"89","author":"Oh","year":"2011","journal-title":"J. Anim. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1096\/fj.201800784R","article-title":"Disruption of G(0)\/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken","volume":"33","author":"Park","year":"2019","journal-title":"FASEB J."},{"key":"ref_53","first-page":"5358","article-title":"Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility","volume":"62","author":"Gillan","year":"2002","journal-title":"Cancer Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1080\/10495398.2016.1169191","article-title":"Associations of the Novel Polymorphisms of Periostin and Platelet-Derived Growth Factor Receptor-Like Genes with Egg Production Traits in Local Chinese Dagu Hens","volume":"27","author":"Jing","year":"2016","journal-title":"Anim. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1161\/CIRCRESAHA.120.316943","article-title":"Periostin: A Potential Therapeutic Target for Pulmonary Hypertension?","volume":"127","author":"Nie","year":"2020","journal-title":"Circ. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1002\/bab.1193","article-title":"Periostin induces chemoresistance in colon cancer cells through activation of the PI3K\/Akt\/survivin pathway","volume":"62","author":"Xiao","year":"2015","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1186\/s12935-020-01342-4","article-title":"MicroRNAs signatures, bioinformatics analysis of miRNAs, miRNA mimics and antagonists, and miRNA therapeutics in osteosarcoma","volume":"20","author":"Otoukesh","year":"2020","journal-title":"Cancer. Cell Int."},{"key":"ref_58","first-page":"777","article-title":"Angiogenesis and reproduction","volume":"108","author":"Smith","year":"2001","journal-title":"Br. J. Obstet. Gynaecol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Chen, X., Sun, X., Chimbaka, I.M., Qin, N., Xu, X., Liswaniso, S., Xu, R., and Gonzalez, J.M. (2021). Transcriptome Analysis of Ovarian Follicles Reveals Potential Pivotal Genes Associated with Increased and Decreased Rates of Chicken Egg Production. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.622751"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7696(06)49001-X","article-title":"Endogenous ligands of PACAP\/VIP receptors in the autocrine-paracrine regulation of the adrenal gland","volume":"249","author":"Conconi","year":"2006","journal-title":"Int. Rev. Cytol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Sun, X., Chen, X., Zhao, J., Ma, C., Yan, C., Liswaniso, S., Xu, R., and Qin, N. (2021). Transcriptome comparative analysis of ovarian follicles reveals the key genes and signaling pathways implicated in hen egg production. BMC Genom., 22.","DOI":"10.1186\/s12864-021-08213-w"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/18\/2747\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:02:48Z","timestamp":1760112168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/18\/2747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,23]]},"references-count":61,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ani14182747"],"URL":"https:\/\/doi.org\/10.3390\/ani14182747","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,23]]}}}