{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:23:51Z","timestamp":1793042631868,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Costa Rica Institute of Technology","award":["VIE-2151083"],"award-info":[{"award-number":["VIE-2151083"]}],"id":[{"id":"https:\/\/ror.org\/04zhrfn38","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Costa Rica Institute of Technology","award":["Agroalnext\/2022\/063"],"award-info":[{"award-number":["Agroalnext\/2022\/063"]}],"id":[{"id":"https:\/\/ror.org\/04zhrfn38","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Costa Rica Institute of Technology","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}],"id":[{"id":"https:\/\/ror.org\/04zhrfn38","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Postgraduate Office of ITCR","award":["VIE-2151083"],"award-info":[{"award-number":["VIE-2151083"]}]},{"name":"Postgraduate Office of ITCR","award":["Agroalnext\/2022\/063"],"award-info":[{"award-number":["Agroalnext\/2022\/063"]}]},{"name":"Postgraduate Office of ITCR","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}]},{"name":"AGROALNEXT programme","award":["VIE-2151083"],"award-info":[{"award-number":["VIE-2151083"]}]},{"name":"AGROALNEXT programme","award":["Agroalnext\/2022\/063"],"award-info":[{"award-number":["Agroalnext\/2022\/063"]}]},{"name":"AGROALNEXT programme","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}]},{"name":"European Union NextGenerationEU","award":["VIE-2151083"],"award-info":[{"award-number":["VIE-2151083"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Union NextGenerationEU","award":["Agroalnext\/2022\/063"],"award-info":[{"award-number":["Agroalnext\/2022\/063"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Union NextGenerationEU","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein and Jersey breeds were used, and nine frozen-thawed semen doses per animal were analyzed for each breed. Three temperatures (35, 37, and 40 \u00b0C) and three thawing times (35, 40, and 45 s) were evaluated using a factorial design. Motility and kinematic patterns were analyzed using CASA-mot (Computer-Assisted Semen Analysis of motility) technology at different post-thawing times (0.5, 1, and 2 h). Sperm motility in Jersey bulls was higher (p &lt; 0.05) than in Holstein ones (64.52 \u00b1 1.45% and 53.10 \u00b1 1.40%, respectively). The same effect was seen with progressive motility among the two breeds (Jersey: 45.29 \u00b1 1.00%; Holstein: 36.30 \u00b1 0.98%, p &lt; 0.05). The Jersey breed presented higher values (p &lt; 0.05) of curvilinear velocity (VCL), rectilinear velocity (VSL), average velocity (VAP), linearity on forward progression (LIN), and wobble (WOB). The Holstein breed showed a lower mean value (p &lt; 0.05) of the beat-cross frequency (BCF) compared to the Jersey breed, thus suggesting an effect on VCL and VAP. During the post-thaw period, a gradual increase in VCL was observed at 2 h. VSL and VAP showed a decrease (p &lt; 0.05) as the post-thaw period was prolonged. The study showed differences in sperm quality between Holstein and Jersey breeds, influenced by cryopreservation, thawing, and post-thawing incubation. Thawing at 37 \u00b0C for 30 s was considered optimal in relation to sperm motility. In addition, a decrease in sperm quality was observed as post-thawing time increased.<\/jats:p>","DOI":"10.3390\/ani14192768","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T16:16:37Z","timestamp":1727280997000},"page":"2768","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds"],"prefix":"10.3390","volume":"14","author":[{"given":"Juan M.","family":"Sol\u00eds","sequence":"first","affiliation":[{"name":"Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1480-4141","authenticated-orcid":false,"given":"Francisco","family":"Sevilla","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9260-5792","authenticated-orcid":false,"given":"Miguel A.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia, Campus Burjassot, C\/Dr Moliner, 50, 46100 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4292-2287","authenticated-orcid":false,"given":"Ignacio","family":"Araya-Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7545-4248","authenticated-orcid":false,"given":"Eduardo R. S.","family":"Roldan","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9840-0058","authenticated-orcid":false,"given":"Alejandro","family":"Sabor\u00edo-Montero","sequence":"additional","affiliation":[{"name":"Alfredo Volio Mata Experimental Station, Faculty of Agri-Food Sciences, University of Costa Rica, Cartago 30304, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3191-6965","authenticated-orcid":false,"given":"Anthony","family":"Valverde","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.22358\/jafs\/127691\/2020","article-title":"The Application of Computer-Assisted Semen Analysis (CASA) Technology to Optimise Semen Evaluation. 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