{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:04:35Z","timestamp":1781586275283,"version":"3.54.5"},"reference-count":49,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"City-School Cooperation Project of the Special Funds of Science and Technology in Fuyang City undertaken by Fuyang Normal University","award":["SXHZ2020007"],"award-info":[{"award-number":["SXHZ2020007"]}]},{"name":"City-School Cooperation Project of the Special Funds of Science and Technology in Fuyang City undertaken by Fuyang Normal University","award":["092GJHZ2022044FN"],"award-info":[{"award-number":["092GJHZ2022044FN"]}]},{"name":"Future Partner Special Fund of the Chinese Academy of Sciences","award":["SXHZ2020007"],"award-info":[{"award-number":["SXHZ2020007"]}]},{"name":"Future Partner Special Fund of the Chinese Academy of Sciences","award":["092GJHZ2022044FN"],"award-info":[{"award-number":["092GJHZ2022044FN"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Animals"],"abstract":"<jats:p>This study aimed to investigate the effects of fermented blueberry pomace (FBP) on the intestinal barrier function and cecal microbiome of aged laying hens. A total of 320 Yukou Jingfen No. 8 laying hens (345-day-old) were randomly divided into a control group, 0.25% FBP group, 0.5% FBP group, or 1.0% FBP group. The results showed that the villus height (VH) in the jejunum of the 0.25\u20130.5% FBP groups and villus surface area in the jejunum of the 0.25% FBP group were higher (p &lt; 0.05), while 0.25% FBP supplementation displayed a higher (p = 0.070) VH in the ileum compared to the control group. Mucin-2 expression was upregulated (p &lt; 0.05) in the jejunum of the 0.5% FBP group and the ileum of the 0.25\u20130.5% FBP groups. Compared to the control group, interleukin (IL)-4 and IL-13 expressions were upregulated (p &lt; 0.05) in the 1.0% FBP group. Microbiota analysis revealed that Prevotella abundance in the cecum of the 0.5\u20131.0% FBP groups was higher (p &lt; 0.05) than in the 0.25% FBP group. In addition, microbial function prediction analysis showed that cecal microbiota in the 0.25% FBP group were mainly enriched by alanine\/aspartate\/glutamate metabolism and methane metabolism. Moreover, Spearman\u2019s correlation analysis revealed the potential correlations between the abundance of the cecal microbiota and intestinal-barrier-function-related gene expressions, as well as the short-chain fatty acid content, of laying hens. In summary, dietary FBP supplementation enhanced intestinal barrier function by improving intestinal morphology, upregulating gene expressions related to barrier function, and altering the cecal microbiota of aged laying hens.<\/jats:p>","DOI":"10.3390\/ani14192786","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T09:46:20Z","timestamp":1727343980000},"page":"2786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dietary Fermented Blueberry Pomace Supplementation Improves Small Intestinal Barrier Function and Modulates Cecal Microbiota in Aged Laying Hens"],"prefix":"10.3390","volume":"14","author":[{"given":"Binghua","family":"Qin","sequence":"first","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"},{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadong","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0118-5708","authenticated-orcid":false,"given":"Md. Abul Kalam","family":"Azad","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8034-6682","authenticated-orcid":false,"given":"Xiangfeng","family":"Kong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"},{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1079\/WPS200445","article-title":"The role of the commensal gut microbial community in broiler chickens","volume":"61","author":"Lan","year":"2005","journal-title":"Worlds Poult. 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