{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:36:49Z","timestamp":1787683009381,"version":"build-2784847793"},"reference-count":62,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic and Slovak Academy of Sciences VEGA","award":["1\/0731\/21"],"award-info":[{"award-number":["1\/0731\/21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Aeromonas spp. is a major pathogen in aquaculture with a great negative economic impact. Essential oils (EOs) are compounds of the secondary metabolism of plants known for their antibacterial and antibiofilm activities. In this study, in vitro antibacterial activity of eight EOs: tea tree (extracted from Melaleuca alternifolia), eucalyptus (extracted from Eucalyptus globulus LABILL.), knee timber (extracted from Pini mungo L.), peppermint (extracted from Mentha piperita L.), oregano (extracted from Origanum vulgare L.), rosemary (extracted from Rosmarinus officinalis L.), thyme (extracted from Thymus vulgaris L.) and pine EO (extracted from Pinus silvestris L.), obtained from Calendula a.s., was evaluated. Their antibacterial activity was demonstrated against Aeromonas spp. isolates. Oregano and thyme EOs showed the strongest activity against all tested isolates at low concentrations, followed by tea tree and peppermint EOs. The MIC value ranged from 0.06 \u00b5L\/mL to 1.0 \u00b5L\/mL. The tested EOs showed a significant antibiofilm activity against biofilm-forming isolates with MBIC50 ranging from 0.015 \u00b5L\/mL to 0.25 \u00b5L\/mL. All tested isolates were obtained from rainbow trout free of clinical signs of infection. Twelve isolates of Aeromonas salmonicida subsp. masoucida, four Aeromonas hydrophila, and four isolates of Aeromonas veronii were identified. The results of the in vitro study showed a significant effect of EOs against Aeromonas spp., which confirmed their potential for use in aquaculture as a prevention against bacterial diseases and a way of reducing the use of antibiotics.<\/jats:p>","DOI":"10.3390\/ani14223202","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T04:02:54Z","timestamp":1731038574000},"page":"3202","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibacterial and Antibiofilm Activity of Essential Oils Against Aeromonas spp. Isolated from Rainbow Trout"],"prefix":"10.3390","volume":"14","author":[{"given":"Patr\u00edcia","family":"Hudecov\u00e1","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0763-831X","authenticated-orcid":false,"given":"Jana","family":"Ko\u0161\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda","family":"Hajdu\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1n","family":"Kir\u00e1ly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1638-4198","authenticated-orcid":false,"given":"Peter","family":"Hor\u0148ak","sequence":"additional","affiliation":[{"name":"Institute of Materials and Quality Engineering, Faculty of Materials, Metallurgy and Recycling, Technical University in Ko\u0161ice, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"ref_1","unstructured":"Shahbandeh, M. 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