{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:26:32Z","timestamp":1791905192882,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFD1800702"],"award-info":[{"award-number":["2022YFD1800702"]}]},{"name":"National Key Research and Development Program of China","award":["CARS37"],"award-info":[{"award-number":["CARS37"]}]},{"name":"National Key Research and Development Program of China","award":["234200510017"],"award-info":[{"award-number":["234200510017"]}]},{"name":"China Agriculture Research System of MOF and MARA (Beef\/Yak)","award":["2022YFD1800702"],"award-info":[{"award-number":["2022YFD1800702"]}]},{"name":"China Agriculture Research System of MOF and MARA (Beef\/Yak)","award":["CARS37"],"award-info":[{"award-number":["CARS37"]}]},{"name":"China Agriculture Research System of MOF and MARA (Beef\/Yak)","award":["234200510017"],"award-info":[{"award-number":["234200510017"]}]},{"name":"Leading Talents of Science and Technology in the Central Plain of China","award":["2022YFD1800702"],"award-info":[{"award-number":["2022YFD1800702"]}]},{"name":"Leading Talents of Science and Technology in the Central Plain of China","award":["CARS37"],"award-info":[{"award-number":["CARS37"]}]},{"name":"Leading Talents of Science and Technology in the Central Plain of China","award":["234200510017"],"award-info":[{"award-number":["234200510017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), which presents worldwide prevalence. BLV caused substantial economic loss in China around the 1980s; then, it could not be detected for some time, until recently. Due to its latent and chronic characteristics, the efficient and accurate detection of BLV is of utmost significance to the timely implementation of control measures. Therefore, this study harnessed the recombinase-aided amplification (RAA), clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 13a (Cas13a) technology, and lateral flow (LF) strips to develop an efficient method for detection of BLV. In this method, isothermal amplification of the targeted pol gene is performed at 37 \u00b0C with a detection threshold of 1 copy\/\u00b5L, and the procedure is completed in 100 min. This assay demonstrated high selectivity for BLV, as indicated by the absence of a cross-reaction with six common bovine pathogens. Remarkably, 100 blood samples from dairy cows were tested in parallel with a conventional quantitative polymerase chain reaction (qPCR) and this method and the results showed 100% agreement. Furthermore, this method exhibited good repeatability. In conclusion, in this study, we established a sensitive and specific method for BLV detection, which shows promise for application in BLV surveillance.<\/jats:p>","DOI":"10.3390\/ani14223262","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T06:23:16Z","timestamp":1731478996000},"page":"3262","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["CRISPR-Cas13a-Based Lateral Flow Assay for Detection of Bovine Leukemia Virus"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2080-3412","authenticated-orcid":false,"given":"Yuxi","family":"Zhao","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Seed Industry Development Center, Zhengzhou 450045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1200-5314","authenticated-orcid":false,"given":"Yingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changmin","family":"Hu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aizhen","family":"Guo","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Key Laboratory of Ruminant Bio-Products, China Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6080","DOI":"10.3390\/v7112929","article-title":"Recent Advances in BLV Research","volume":"7","author":"Barez","year":"2015","journal-title":"Viruses"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.3390\/v3071210","article-title":"Preventive and therapeutic strategies for bovine leukemia virus: Lessons for HTLV","volume":"3","author":"Florins","year":"2011","journal-title":"Viruses"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3168\/jdsc.2021-0187","article-title":"The 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