{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:07:53Z","timestamp":1792195673443,"version":"4.4.0"},"reference-count":40,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Minister of Science and Higher Education"},{"name":"the Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Poland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The vaginal bacteria are critical for neonatal immunity, as well as for further infections and pathologies in foals and mares during the postpartum period. The vaginal microbiota was examined in six mares. Swabs were taken from the vaginal caudal wall within 12 h after natural delivery. Next-generation sequencing was used to analyze the composition of the vaginal microbiota and identify shared bacterial taxa. The post-delivery vaginal microbiome of mares included 18 identified families, 25 genera, and 33 species. The core families of the mares\u2019 microbiome comprised Leptotrichiaceae (21.72%), Peptostreptococcaceae\/Tisserellales (15.54%), Corynebacteriaceae (13.32%), Aerococcaceae (10.84%), and Campylobacteraceae (4.26%). Shared bacterial species included Corynebacterium kutsheri, Campylobacter spp., Facklamia spp., and Oceanivirga uncultured bacteria. We found the presence of various Corynebacterium and Streptococcus species that could be involved in uterine disorders. Further research should focus on the possible pathogenicity of these bacteria for mares in the postpartum period and newborn foals.<\/jats:p>","DOI":"10.3390\/ani14223337","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T03:57:04Z","timestamp":1732075024000},"page":"3337","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Vaginal Microbiome of Mares on the Post-Foaling Day Under Field Conditions"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7621-4449","authenticated-orcid":false,"given":"Katarzyna","family":"P\u0142oneczka-Janeczko","sequence":"first","affiliation":[{"name":"Department of Epizootiology with Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Plac Grunwaldzki 45, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2239-7155","authenticated-orcid":false,"given":"Marcin","family":"Magdziarz","sequence":"additional","affiliation":[{"name":"Hugo Steinhaus Center, Faculty of Pure and Applied Mathematics, Wroc\u0142aw University of Science and Technology, Wyspia\u0144skiego 27, 50-370 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5117-9659","authenticated-orcid":false,"given":"Marta","family":"Siemieniuch-Tartanus","sequence":"additional","affiliation":[{"name":"Department of Large Animals Diseases with the Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 100, 02-787 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s10815-015-0614-z","article-title":"Endometrial microbiome at the time of embryo transfer: Next-generation sequencing of the 16S ribosomal subunit","volume":"33","author":"Franasiak","year":"2016","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e1602","DOI":"10.7717\/peerj.1602","article-title":"Characterisation of the human uterine microimicrobiome in non-pregnant women through deep sequencing of the V1-2 region of the 16S rRNA gene","volume":"4","author":"Verstraelen","year":"2016","journal-title":"PeerJ"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"254le4","DOI":"10.1126\/scitranslmed.3009864","article-title":"Comment on the placenta harbors a unique microbiome","volume":"6","author":"Kliman","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e00933-19","DOI":"10.1128\/mSphere.00933-19","article-title":"No consistent evidence for microbiota in murine placental and fetal tissues","volume":"5","author":"Theis","year":"2020","journal-title":"mSphere"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Barba, M., Mart\u00ednez-Bov\u00ed, R., Quereda, J.J., Moc\u00e9, M.L., Plaza-D\u00e1vila, M., Jim\u00e9nez-Trigos, E., G\u00f3mez-Mart\u00edn, \u00c1., Gonz\u00e1lez-Torres, P., Carbonetto, B., and Garc\u00eda-Rosell\u00f3, E. (2020). Vaginal microbiota is stable throughout the estrous cycle in Arabian mares. Animals, 10.","DOI":"10.3390\/ani10112020"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Beckers, K.F., Gomes, V.C.L., Crissman, C.R., Liu, C.C., Schulz, C.J., Childers, G.W., and Sones, J.L. (2023). Metagenetic Analysis of the Pregnant Microbiome in Horses. Animals, 13.","DOI":"10.3390\/ani13121999"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jevs.2018.05.156","article-title":"Metagenetic Characterization of the Resident Equine Uterine Microbiome Using Multiple Techniques","volume":"66","author":"Heil","year":"2018","journal-title":"J. Equine Vet. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gil-Miranda, A., Macnicol, J., Orellana-Guerrero, D., Samper, J.C., and Gomez, D.E. (2024). Reproductive Tract Microbiota of Mares. Vet. Sci., 11.","DOI":"10.3390\/vetsci11070324"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.theriogenology.2023.09.011","article-title":"Bacteria in the healthy equine vagina during the estrous cycle","volume":"213","author":"Malaluang","year":"2024","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Malaluang, P., Wil\u00e9n, E., Frosth, S., Lindahl, J., Hansson, I., and Morrell, J.M. (2022). Vaginal Bacteria in Mares and the Occurrence of Antimicrobial Resistance. Microorganisms, 10.","DOI":"10.3390\/microorganisms10112204"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0140-6736(08)60074-4","article-title":"Epidemiology and causes of preterm birth","volume":"371","author":"Goldenberg","year":"2008","journal-title":"Lancet"},{"key":"ref_12","first-page":"1","article-title":"Child health neonatal infections: Group B streptococcus","volume":"1","author":"Hanley","year":"2008","journal-title":"BMJ Clin. Evid."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Schlinzig, T., Johansson, S., Stephansson, O., Hammarstr\u00f6m, L., Zetterstr\u00f6m, R.H., von D\u00f6beln, U., Cnattingius, S., and Norman, M. (2017). Surge of immune cell formation at birth differs by mode of delivery and infant characteristics\u2014A population-based cohort study. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0184748"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1159\/000111102","article-title":"Mode of delivery-effects on gut microbiota and humoral immunity","volume":"93","author":"Huurre","year":"2008","journal-title":"Neonatology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jri.2003.11.007","article-title":"Comparison of decidual leukocytes following spontaneous vaginal delivery and elective cesarean section in uncomplicated human term pregnancy","volume":"62","author":"Scherjon","year":"2004","journal-title":"J. Reprod. Immunol."},{"key":"ref_16","unstructured":"McKinnon, A.O., Squires, E.L., Vaala, W.E., and Varner, D.D. (2011). Assessment and modification of passive transfer. Equine Reproduction, Wiley-Backwell. [2nd ed.]."},{"key":"ref_17","first-page":"1299","article-title":"Passive immunity in the foal: Measurement of immunoglobulin classes and specific antibody","volume":"34","author":"McGuire","year":"1973","journal-title":"Am. J. Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.2527\/jas.2014-8496","article-title":"Physiology and Endocrinology Symposium: Uterine infection: Linking infection and innate immunity with infertility in the high-producing dairy cow","volume":"93","author":"Bromfield","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e13603","DOI":"10.1111\/asj.13603","article-title":"Investigations of the maintenance system of the Konik Polski horse and its effects on fecal microbiota activity during the winter and summer seasons","volume":"92","author":"Fotschki","year":"2021","journal-title":"Anim. Sci. J."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Verbanic, S., Kim, C.Y., Deacon, J.M., and Chen, I.A. (2019). Improved single-swab sample preparation for recovering bacterial and phage DNA from human skin and wound microbiomes. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1586-4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Satora, M., Magdziarz, M., Rz\u0105sa, A., Rypu\u0142a, K., and P\u0142oneczka-Janeczko, K. (2020). Insight into intestinal microbiome of farrowing sows following the administration of garlic (Alium sativum) extract and probiotic bacteria cultures under farming conditions. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02659-y"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1128\/aem.62.2.340-346.1996","article-title":"Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community","volume":"62","author":"Ferris","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.2006.0030-1299.14714.x","article-title":"Entropy and diversity","volume":"113","author":"Jost","year":"2006","journal-title":"Oikos"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15485","DOI":"10.1073\/pnas.1205147109","article-title":"Microbial shifts in the swine distal gut in response to the treatment with antimicrobial growth promoter, tylosin","volume":"109","author":"Kim","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Agostinis, C., Mangogna, A., Bossi, F., Ricci, G., Kishore, U., and Bulla, R. (2019). Uterine Immunity and Microbiota: A Shifting Paradigm. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.02387"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1126\/science.1223490","article-title":"Interactions between the microbiota and the immune system","volume":"336","author":"Hooper","year":"2012","journal-title":"Science"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kronfeld, H., Kemper, N., and H\u00f6lzel, C.S. (2022). Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows. Agriculture, 12.","DOI":"10.3390\/agriculture12030405"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1439-0531.2010.01634.x","article-title":"Advances in the diagnosis and treatment of chronic infectious and post\u2013mating-induced endometritis in the mare","volume":"45","author":"LeBlanc","year":"2010","journal-title":"Reprod. Domest. Anim."},{"key":"ref_29","first-page":"490","article-title":"Comprehensive review on equine placentitis","volume":"61","author":"Canisso","year":"2015","journal-title":"Proc. Am. Assoc. Equine Pract."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Koroleva, E., Steffen, H.C., Bosch, C., Flemming, L., and Botha, A. (2022). Physiological characterisation of Corynebacterium uterequi associated with pregnancy complications in mares. Vet. Microbiol., 273.","DOI":"10.1016\/j.vetmic.2022.109522"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.vetmic.2013.03.025","article-title":"Corynebacterium uterequi sp. nov., a non-lipophilic bacterium isolated from urogenital samples from horses","volume":"165","author":"Hoyles","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1177\/104063879901100618","article-title":"Normal bacterial flora in canine and feline uteri","volume":"11","author":"Schultheiss","year":"1999","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1128\/JCM.00796-12","article-title":"The genus corynebacterium and other medically relevant coryneform-like bacteria","volume":"50","author":"Bernard","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","first-page":"733","article-title":"Uterine involution, ovarian activity and fertility in the post-partum mare","volume":"35","author":"Koskinen","year":"1987","journal-title":"J. Reprod. Fertil."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0737-0806(89)80020-6","article-title":"Aerobic and anaerobic bacterial flora in the post-partum mare","volume":"9","author":"Purswell","year":"1989","journal-title":"Equine Vet. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4680","DOI":"10.1073\/pnas.1002611107","article-title":"Vaginal microbiome of reproductive-age women","volume":"108","author":"Ravel","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.tvjl.2015.03.018","article-title":"Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments in horses","volume":"205","author":"Costa","year":"2015","journal-title":"Vet. J."},{"key":"ref_38","first-page":"452","article-title":"Metagenomic analysis of the equine placental microbiome","volume":"9","author":"Xia","year":"2017","journal-title":"Clin. Theriogenology"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Coscia, A., Bardanzellu, F., Caboni, E., Fanos, V., and Peroni, D.G. (2021). When a Neonate Is Born, So Is a Microbiota. Life, 11.","DOI":"10.3390\/life11020148"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.joco.2018.03.002","article-title":"The newborne conjunctival flora at the post delivery 24-hours","volume":"30","author":"Kara","year":"2018","journal-title":"J. Curr. Ophtalmol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/22\/3337\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:35:39Z","timestamp":1760114139000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/22\/3337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,20]]},"references-count":40,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["ani14223337"],"URL":"https:\/\/doi.org\/10.3390\/ani14223337","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,20]]}}}