{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:28:21Z","timestamp":1787981301975,"version":"build-2784847793"},"reference-count":121,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Province of Southern Sardinia"},{"name":"Metropolitan City of Cagliari"},{"name":"Province of Sassari"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>We adopted a morphometric approach to provide statistical support for the description of two different morphotypes (I, reproductive, II, non-reproductive) firstly observed in Procambarus clarkii specimens caught in a population from Sardinia Island (western Mediterranean). The morphometric study was preceded by molecular taxonomic identification using the mitochondrial Cytochrome C Oxidase subunit I (COI) gene. The presence or absence of the pathogen Aphanomyces astaci, responsible for the plague, was also investigated using the ribosomal Internal Transcribed Spacer (ITS) marker. The estimation of the size at onset of maturity (SOM) was further performed in males. Finally, the population structure was examined. Our results confirmed the entirety of the individuals as P. clarkii, and the absence of the pathogen A. astaci. Morphological analysis revealed a significant correlation between chela and carapace lengths, while intra- and inter-sexual dimorphism in the chela length confirmed the occurrence of the two morphotypes. The SOM in males was established between 35.0 and 37.1 mm carapace length. Finally, the size-population structure showed a Gaussian distribution. The recognition of the two distinct morphotypes in the crayfish could be useful as an additional tool not only to identify the reproductive period of this invasive crustacean but also to enrich the guidelines for the correct taxonomic identification of the species.<\/jats:p>","DOI":"10.3390\/ani14243558","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T08:40:10Z","timestamp":1733820010000},"page":"3558","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["New Insight into the Crayfish Procambarus clarkii (Girard, 1852) (Crustacea, Cambaridae): A Morphometric Combined Approach to Describe the Case of a Mediterranean Population"],"prefix":"10.3390","volume":"14","author":[{"given":"Noemi","family":"Pascale","sequence":"first","affiliation":[{"name":"Department of Chemical, Physical, Mathematical and Natural Sciences, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"},{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilenia","family":"Azzena","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Locci","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"},{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/b, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Deplano","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/b, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio","family":"Orr\u00f9","sequence":"additional","affiliation":[{"name":"GRAIA\u2014Gestione Ricerca Ambientale Ittica Acque, Via Repubblica 1, 21020 Varano Borghi, VA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesare","family":"Puzzi","sequence":"additional","affiliation":[{"name":"GRAIA\u2014Gestione Ricerca Ambientale Ittica Acque, Via Repubblica 1, 21020 Varano Borghi, VA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Are","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3501-714X","authenticated-orcid":false,"given":"Fabio","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/b, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3058-4663","authenticated-orcid":false,"given":"Daria","family":"Sanna","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43\/b, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5042-4851","authenticated-orcid":false,"given":"Marco","family":"Casu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Galil, B.S. 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