{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T23:49:55Z","timestamp":1793922595680,"version":"4.8.0"},"reference-count":46,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T00:00:00Z","timestamp":1734912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research and Innovation Institute of Excellence, Walailak University","award":["WU66266"],"award-info":[{"award-number":["WU66266"]}],"id":[{"id":"https:\/\/ror.org\/04b69g067","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Multiple blood pathogen infections are increasingly found in many areas, particularly in tropical regions. This study aimed to investigate the effectiveness and safety of using doxycycline monotherapy to treat triple blood pathogen infection in sheltered dogs. A total of 375 sheltered dogs were screened for blood pathogen using polymerase chain reaction technique (PCR). There were 34 dogs with triple infection (Babesia vogeli, Ehrlichia canis, and Anaplasma platys), and most dogs displayed anemia and thrombocytopenia. These dogs were treated with doxycycline (10 mg\/kg\/day) orally for four weeks, and their blood profiles were monitored. Almost all pathogens were undetectable by PCR by day 14 and pathogens were all cleared by day 28. Most blood profiles significantly improved after 14 days. The improvement continued after 28 days. The red blood cell and platelet count (PLT) were increased in similar trends between mild-to-moderate and severe thrombocytopenia groups. The average PLT in the mild-to-moderate thrombocytopenia group raised to normal level by day 14, whereas in the severe group, it was gradually increased to normal level by day 70. We propose using doxycycline monotherapy for 28 days to eliminate all pathogens, and facilitate recovery and welfare in dogs suffering with triple blood pathogen infections.<\/jats:p>","DOI":"10.3390\/ani14243714","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T10:58:55Z","timestamp":1734951535000},"page":"3714","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Improvements in Blood Profiles of Canines Naturally Infected with Triple Blood Pathogens (Babesia vogeli, Ehrlichia canis, and Anaplasma platys) Subsequent to Doxycycline Monotherapy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6948-4144","authenticated-orcid":false,"given":"Tuempong","family":"Wongtawan","sequence":"first","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Centre, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence Research for Melioidosis and Other Microorganism, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0216-2380","authenticated-orcid":false,"given":"Narin","family":"Sontigun","sequence":"additional","affiliation":[{"name":"Office of Administrative Interdisciplinary Program on Agricultural Technology, School of Agricultural Technology, King Mongkut\u2019s Institute of Technology Ladkrabang, Bangkok 10520, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5140-429X","authenticated-orcid":false,"given":"Kanpapat","family":"Boonchuay","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Centre, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2269-5734","authenticated-orcid":false,"given":"Phatcharaporn","family":"Chiawwit","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Centre, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9443-0831","authenticated-orcid":false,"given":"Oraphan","family":"Wongtawan","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7833-3161","authenticated-orcid":false,"given":"Orachun","family":"Hayakijkosol","sequence":"additional","affiliation":[{"name":"Division of Tropical Health and Medicine, College of Public Health, Medical and Veterinary Sciences, James Cook University, Brisbane City, QLD 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0002-1448","authenticated-orcid":false,"given":"Worakan","family":"Boonhoh","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Centre, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Innovation on Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.3201\/eid2606.191832","article-title":"Zoonotic vector-borne pathogens and ectoparasites of dogs and cats in Eastern and Southeast Asia","volume":"26","author":"Colella","year":"2020","journal-title":"Emerg. 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