{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T11:41:00Z","timestamp":1774698060737,"version":"3.50.1"},"reference-count":74,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T00:00:00Z","timestamp":1734998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland","award":["15\/FBW\/2024"],"award-info":[{"award-number":["15\/FBW\/2024"]}]},{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland","award":["OR14-6521.2-OR1400004\/18"],"award-info":[{"award-number":["OR14-6521.2-OR1400004\/18"]}]},{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland","award":["Z-013"],"award-info":[{"award-number":["Z-013"]}]},{"name":"Agency for Restructuring and Modernisation of Agriculture","award":["15\/FBW\/2024"],"award-info":[{"award-number":["15\/FBW\/2024"]}]},{"name":"Agency for Restructuring and Modernisation of Agriculture","award":["OR14-6521.2-OR1400004\/18"],"award-info":[{"award-number":["OR14-6521.2-OR1400004\/18"]}]},{"name":"Agency for Restructuring and Modernisation of Agriculture","award":["Z-013"],"award-info":[{"award-number":["Z-013"]}]},{"name":"National Inland Fisheries Research Institute, Olsztyn, Poland","award":["15\/FBW\/2024"],"award-info":[{"award-number":["15\/FBW\/2024"]}]},{"name":"National Inland Fisheries Research Institute, Olsztyn, Poland","award":["OR14-6521.2-OR1400004\/18"],"award-info":[{"award-number":["OR14-6521.2-OR1400004\/18"]}]},{"name":"National Inland Fisheries Research Institute, Olsztyn, Poland","award":["Z-013"],"award-info":[{"award-number":["Z-013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also investigated. After egg collection, the Pseudo-Gonado-Somatic Index (PGSI) was measured; the PGSI values in the samples obtained using the pneumatic method (13.8 \u00b1 3.9%) were lower, but did not differ statistically from those obtained by hand stripping (16.5 \u00b1 5.4%). The 0.4% NaCl solution proved to be the most effective for sperm activation, as assessed by the Computer-Assisted Sperm Analysis (CASA) system, compared to the 0.8% NaCl solution. The pneumatic method achieved a higher egg collection efficiency (93.7% occlusion) than the traditional method, with significant differences observed in groups activated with water. The average hatching percentage of larvae was 89.5% in groups using the pneumatic method, compared to 71.2% in the traditional groups, highlighting the advantages of this modern approach. The application of the pneumatic method and 0.4% NaCl for the artificial fertilization of northern pike resulted in higher fertilization and hatching rates compared to other techniques, making this method a promising option for the artificial reproduction of other fish species.<\/jats:p>","DOI":"10.3390\/ani15010008","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T09:48:58Z","timestamp":1735033738000},"page":"8","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (Esox lucius)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0057-7188","authenticated-orcid":false,"given":"Micha\u0142","family":"Cydzik","sequence":"first","affiliation":[{"name":"Department of Aquaculture, National Inland Fisheries Research Institute, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krystyna","family":"Demska-Zak\u0119\u015b","sequence":"additional","affiliation":[{"name":"Department of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miros\u0142aw","family":"Szczepkowski","sequence":"additional","affiliation":[{"name":"Department of Sturgeon Fish Breeding, National Inland Fisheries Research Institute, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u017cena","family":"Szczepkowska","sequence":"additional","affiliation":[{"name":"Department of Sturgeon Fish Breeding, National Inland Fisheries Research Institute, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-7351","authenticated-orcid":false,"given":"Beata","family":"Sarosiek","sequence":"additional","affiliation":[{"name":"Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Blitek","sequence":"additional","affiliation":[{"name":"Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksy","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical University of Bialystok; 15-089, Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0251-8626","authenticated-orcid":false,"given":"Rados\u0142aw Kajetan","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mylonas, C., and Zohar, Y. 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