{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:35:17Z","timestamp":1759970117421,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T00:00:00Z","timestamp":1736035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfeicoamento de Pessoal de N\u00edvel Superior (CAPES), Brazil","award":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"]}]},{"name":"Fundac\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazil","award":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Brazil","award":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","E-26\/200.791\/2020","305956\/2021-3","316975\/2023-0","307067\/2023-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Zoonotic visceral leishmaniasis is caused by Leishmania (Leishmania) infantum and dogs are the main domestic reservoir. This study compared the performance of parasitological tests using semi-automatic needle puncture (SANP) for collecting popliteal lymph node samples with samples collected from the same lymph node by fine needle aspiration puncture (FNAP) and by necropsy for the diagnosis of canine visceral leishmaniasis (CVL). Popliteal lymph node samples were collected from 30 CVL-seropositive dogs from an endemic region in Brazil. After clinical examination and euthanasia, samples were collected from the same lymph node by SANP, FNAP, and necropsy. The reference tests were culture, immunohistochemistry, and histopathology. Positivity for Leishmania spp. was 70% for immunohistochemistry and 33.3% for histopathology. Culture positivity using the different sampling techniques was 77% for necropsy (87% in the first week), 73% for FNAP (82% in the first week), and 63% for SANP (95% in the first week). The combination of SANP and culture proved to be an alternative for the diagnosis of Leishmania spp. in the lymph node samples of dogs because of its high positivity rate and because it is more practical and faster and has a shorter time to positivity by culture when compared to FNAP and necropsy sampling.<\/jats:p>","DOI":"10.3390\/ani15010107","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T04:37:10Z","timestamp":1736138230000},"page":"107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.3390","volume":"15","author":[{"given":"Artur Augusto Velho","family":"Mendes J\u00fanior","sequence":"first","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano Borges","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Cl\u00e1udio","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Keidel","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Orsini","family":"Ornellas","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adilson Benedito","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Nunes","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance in Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-1922","authenticated-orcid":false,"given":"Luciana de Freitas Campos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance in Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7197-2128","authenticated-orcid":false,"given":"Andreza Pain","family":"Marcelino","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance in Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0614-4713","authenticated-orcid":false,"given":"Sandro Antonio","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1853-3449","authenticated-orcid":false,"given":"Rodrigo Caldas","family":"Menezes","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,5]]},"reference":[{"key":"ref_1","unstructured":"Brasil. Minist\u00e9rio da Sa\u00fade (2014). Secretaria de Vigil\u00e2ncia em Sa\u00fade. Departamento de Vigil\u00e2ncia Epidemiol\u00f3gica. Manual de Vigil\u00e2ncia e Controle da Leishmaniose Visceral\/Minist\u00e9rio da Sa\u00fade, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Departamento de Vigil\u00e2ncia Epidemiol\u00f3gica."},{"key":"ref_2","unstructured":"(2024, July 06). World Health Organization-Leishmaniasis. Available online: https:\/\/www.who.int\/health-topics\/leishmaniasis#tab=tab_1."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.exppara.2019.02.012","article-title":"The diagnosis of canine visceral leishmaniasis in Brazil: Confronting old problems","volume":"199","author":"Nakasone","year":"2019","journal-title":"Exp. 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