{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T13:59:51Z","timestamp":1784210391126,"version":"3.55.0"},"reference-count":143,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Taishan Scholars Program","award":["2023BBB172"],"award-info":[{"award-number":["2023BBB172"]}]},{"name":"Key R&amp;D Program of Hubei Province","award":["2023BBB172"],"award-info":[{"award-number":["2023BBB172"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>As a fundamental taxonomic group within vertebrates, fish represent an invaluable resource for investigating the mechanisms underlying sex determination and differentiation owing to their extensive geographical distribution and rich biodiversity. Within this biological cohort, the processes of sex determination and differentiation are intricately governed by both genetic factors and the complex interplay of environmental cues. While variations in external environmental factors, particularly temperature, can exert a modulatory influence on sex differentiation in fish to a limited degree, genetic factors remain the primary determinants of sexual traits. Hermaphroditic fish display three distinct types of sexual transitions: protandry (male to female), protogyny (female-to-male), bidirectional sex change (both directions serially). These fish, characterized by their unique reproductive strategies and sexual plasticity, serve as exemplary natural models for elucidating the mechanisms of sex differentiation and sexual transitions in fish. The present review delves into the histological dynamics during gonadal development across three types of sequential hermaphroditic fish, meticulously delineating the pivotal characteristics at each stage, from the inception of primordial gonads to sexual specialization. Furthermore, it examines the regulatory genes and associated signaling pathways that orchestrate sex determination and differentiation. By systematically synthesizing these research advancements, this paper endeavors to offer a comprehensive and profound insight into the intricate mechanisms governing sex differentiation in sequential hermaphroditic fish.<\/jats:p>","DOI":"10.3390\/ani15020119","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T06:49:13Z","timestamp":1736232553000},"page":"119","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Understanding Genetic Regulation of Sex Differentiation in Hermaphroditic Fish"],"prefix":"10.3390","volume":"15","author":[{"given":"Junchao","family":"Fang","sequence":"first","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanglve","family":"Li","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyin","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaomu","family":"Hu","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"},{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bachtrog, D., Mank, J.E., Peichel, C.L., Kirkpatrick, M., Otto, S.P., Ashman, T.-L., Hahn, M.W., Kitano, J., Mayrose, I., and Ming, R. (2014). Sex determination: Why so many ways of doing it?. PLoS Biol., 12.","DOI":"10.1371\/journal.pbio.1001899"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1159\/000342009","article-title":"Diversity and plasticity of sex determination and differentiation in fishes","volume":"7","author":"Kobayashi","year":"2013","journal-title":"Sex. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wang, H., Qu, M., Tang, W., Liu, S., and Ding, S. (2022). Transcriptome profiling and expression localization of key sex-related genes in a socially-controlled hermaphroditic clownfish. Amphiprion clarkii. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23169085"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1152\/physrev.00044.2019","article-title":"Sex determination, gonadal sex differentiation, and plasticity in vertebrate species","volume":"101","author":"Nagahama","year":"2021","journal-title":"Physiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1007\/s11427-014-4797-9","article-title":"Genetic basis and biotechnological manipulation of sexual dimorphism and sex determination in fish","volume":"58","author":"Mei","year":"2015","journal-title":"Sci. China Life Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s10126-019-09886-x","article-title":"Characterization of the european sea bass (Dicentrarchus labrax) gonadal transcriptome during sexual development","volume":"21","author":"Ribas","year":"2019","journal-title":"Mar. Biotechnol."},{"key":"ref_7","unstructured":"Zhu, Y.Y. (2012). Screening of the AFLP Marker Linked to the Sex Locus of Oreochromis niloticus and Association Between Polymorphsims of Two Genes and Growth Traits. [Master\u2019s Thesis, Huazhong Agricultural University]."},{"key":"ref_8","first-page":"644","article-title":"Advances in the study of sex differentiation and sexual transition in hermaphroditic fishes","volume":"46","author":"Zhao","year":"2022","journal-title":"J. Fish. China"},{"key":"ref_9","first-page":"51","article-title":"Histological observations on sex reversal in pond-raised Acanthopagrus schlegeli","volume":"41","author":"Chen","year":"2017","journal-title":"Mar. Sci."},{"key":"ref_10","first-page":"67","article-title":"A preliminary study on the process of sex reversal in Eleutheronema tetradactylum","volume":"16","author":"Lan","year":"2020","journal-title":"South China Fish. Sci."},{"key":"ref_11","unstructured":"Yan, Y., Zhu, H.J., Tao, Y.F., Xu, P., and Qiang, J. (2022). Progress in the study of the effect of AMH gene on sex determination in fishes. Heilongjiang Anim. Sci. Vet. Med., 26\u201331."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.aquaculture.2010.06.019","article-title":"Molecular cloning and mRNA expression attern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides)","volume":"306","author":"Luo","year":"2010","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1111\/are.15660","article-title":"Seasonal changes of plasma sex steroids in captive longtooth grouper Epinephelus bruneus (Bloch) in subtropical regions","volume":"53","author":"Masuma","year":"2022","journal-title":"Aquaculture Res."},{"key":"ref_14","unstructured":"Qu, M. (2019). A Comparative Study of Sex Development in Two Hermaphroditic Fishes, Epinephelus akaara and Kryptolebias marmoratus. [Ph.D. Thesis, Xiamen University]."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/jez.2558","article-title":"Estrogenic signaling and sociosexual behavior in wild sex-changing bluehead wrasses, Thalassoma bifasciatum","volume":"337","author":"Prim","year":"2022","journal-title":"J. Exp. Zool. A Ecol. Integr. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1007\/s10695-021-00977-y","article-title":"Comparison of differential expression genes in ovaries and testes of Pearlscale angelfish Centropyge vrolikii based on RNA-Seq analysis","volume":"47","author":"Zhong","year":"2021","journal-title":"Fish Physiol. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s10641-014-0265-4","article-title":"Bidirectional sex change in the magenta dottyback Pictichromis porphyrea: First evidence from the field in Pseudochromidae","volume":"98","author":"Kuwamura","year":"2015","journal-title":"Environ. Biol. Fish."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.yhbeh.2006.01.008","article-title":"Elevated 11-ketotestosterone during paternal behavior in the Bluebanded goby (Lythrypnus dalli)","volume":"49","author":"Rodgers","year":"2006","journal-title":"Horm. Behav."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s10228-008-0054-1","article-title":"Bi-directional sex change and gonad structure in the gobiid fish Trimma yanagitai","volume":"56","author":"Makoto","year":"2009","journal-title":"Ichthyol. Res."},{"key":"ref_20","unstructured":"Chu, G.B. (2018). Functional Studies on the SoxB Family of Genes in Dugesia japonica. [Master\u2019s Thesis, Henan Normal University]."},{"key":"ref_21","unstructured":"Liu, J. (2015). Cloning and Tissue Expression Analysis of cDNA Sequences of Sox4a, Sox4b, Sox4c and Sox7 Genes in Chinese Giant Salamander (Andrias davidianus). [Master\u2019s Thesis, Sichuan Agricultural University]."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Schedina, I.M., Groth, D., Schlupp, I., and Tiedemann, R. (2018). The gonadal transcriptome of the unisexual Amazon molly Poecilia formosa in comparison to its sexual ancestors, Poecilia mexicana and Poecilia latipinna. BMC Genom., 19.","DOI":"10.1186\/s12864-017-4382-2"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.tig.2010.12.002","article-title":"Unisexual reproduction among vertebrates","volume":"27","author":"Neaves","year":"2011","journal-title":"Trends Genet."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Amano, M., Amiya, N., Fukushima, K., Hagio, H., Yamamoto, N., and Sakakura, Y. (2022). Effects of crowding stress on the hypothalamo-pituitary-interrenal axis of the self-fertilizing fish, Kryptolebias marmoratus. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 264.","DOI":"10.1016\/j.cbpa.2021.111110"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/jeb.12333","article-title":"Sex change in plants and animals: A unified perspective","volume":"27","author":"Guevara","year":"2014","journal-title":"J. Evol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"148166","DOI":"10.1016\/j.gene.2024.148166","article-title":"Synchronously sexual maturity in hermaphrodite fish as revealed by transcriptome analysis in Plectropomus leopardus","volume":"901","author":"Ding","year":"2024","journal-title":"Gene"},{"key":"ref_27","unstructured":"Gui, J.F. (2007). Genetic Basis and Artificial Control of Sexuality and Reproduction in Fish, Science Press."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10228-020-00754-6","article-title":"Hermaphroditism in fishes: An annotated list of species, phylogeny, and mating system","volume":"67","author":"Kuwamura","year":"2020","journal-title":"Ichthyol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1159\/000223079","article-title":"Evolutionary perspectives on hermaphroditism in fishes","volume":"3","author":"Avise","year":"2009","journal-title":"Sex. Dev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Casas, L., Saborido-Rey, F., Ryu, T., Michell, C., Ravasi, T., and Irigoien, X. (2016). Sex change in Clownfish: Molecular insights from transcriptome analysis. Sci. Rep., 6.","DOI":"10.1038\/srep35461"},{"key":"ref_31","unstructured":"Han, Y.L. (2019). Study on the Mechanism of amh Gene in Sex Differentiation of Orange-spotted Grouper (Epinephelus coioides). [Ph.D. Thesis, Sun Yat-sen University]."},{"key":"ref_32","unstructured":"Xiao, Y.M., and Liu, J. (1995). Cell biology of the transition from intersexual to male development in the Monopterus albus. J. Fish. China, 297\u2013304."},{"key":"ref_33","unstructured":"Yang, W.Y., Gu, Z.Q., Wang, C.H., and Dong, W.Z. (2004). Preliminary observations on gonadal morphology during sex reversal in the Monopterus albus. Prog. Vet. Med., 113\u2013115."},{"key":"ref_34","first-page":"139","article-title":"Implications of sex regulation in scleractinian fishes for sex reversal in Mastacembelus armatus","volume":"51","author":"Xue","year":"2024","journal-title":"Guangdong Agric. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, M.H., Sun, Y.L., Zhao, J., Shi, H.J., Zeng, S., Ye, K., Jiang, D.N., Zhou, L.Y., Sun, L.N., and Tao, W.J. (2015). A tandem duplicate of Anti-M\u00fcllerian hormone with a missense SNP on the Y chromosome is essential for male sex determination in Nile tilapia, Oreochromis niloticus. PLoS Genet., 11.","DOI":"10.1371\/journal.pgen.1005678"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1534\/genetics.111.137497","article-title":"Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis","volume":"191","author":"Myosho","year":"2012","journal-title":"Genetics"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bao, L.S., Tian, C.X., Liu, S.K., Zhang, Y., Elaswad, A., Yuan, Z.H., Khalil, K., Sun, F.Y., Yang, Y.J., and Zhou, T. (2019). The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fish. BMC Biol., 17.","DOI":"10.1186\/s12915-019-0627-7"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/ng.2890","article-title":"Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle","volume":"46","author":"Chen","year":"2014","journal-title":"Nat. Genet."},{"key":"ref_39","first-page":"12","article-title":"The Karyotype and ZZ-ZO sex chromosomes of Coilia brachygnathus","volume":"6","author":"Hong","year":"1984","journal-title":"Hereditas"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10126-012-9480-1","article-title":"Isolation and characterization of male-specific DNA markers in the rock bream Oplegnathus fasciatus","volume":"15","author":"Xu","year":"2013","journal-title":"Mar. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hofsten, J.V., and Olsson, P.E. (2005). Zebrafish sex determination and differentiation: Involvement of FTZ-F1 genes. Reprod. Biol. Endocrinol., 3.","DOI":"10.1186\/1477-7827-3-63"},{"key":"ref_42","first-page":"1206","article-title":"Progress in the study of genes regulating male sex differentiation and spermatheca development in fishes","volume":"31","author":"Yang","year":"2023","journal-title":"Acta Lab. Anim. Sci. Sin."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Chen, J., Zhu, Z.Y., and Hu, W. (2022). Progress in research on fish sex determining genes. Water Biol. Secur., 1.","DOI":"10.1016\/j.watbs.2022.100008"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1016\/j.cub.2019.04.069","article-title":"A SNP in a Steroidogenic Enzyme Is Associated with Phenotypic Sex in Seriola Fishes","volume":"29","author":"Koyama","year":"2019","journal-title":"Curr. Biol."},{"key":"ref_45","first-page":"2981","article-title":"Progress in the study of genes related to sex determination and differentiation in fishes","volume":"53","author":"Lu","year":"2014","journal-title":"Hubei Agric. Sci."},{"key":"ref_46","first-page":"93","article-title":"Cloning of cDNA and its expression analysis of Sox9 from Scatophagus argus","volume":"50","author":"Chen","year":"2015","journal-title":"Chin. J. Zool."},{"key":"ref_47","first-page":"97","article-title":"Cloning and expression study of sox9 gene of Paralichthys olivaceus","volume":"29","author":"Wen","year":"2011","journal-title":"Adv. Mar. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Domingos, J.A., Budd, A.M., Banh, Q.Q., Goldsbury, J.A., Zenger, K.R., and Jerry, D.R. (2018). Sex-specific dmrt1 and cyp19a1 methylation and alternative splicing in gonads of the protandrous hermaphrodite barramundi. PLoS. ONE, 13.","DOI":"10.1371\/journal.pone.0204182"},{"key":"ref_49","first-page":"275","article-title":"Expression analysis of Amh and SOX9 genes during gonadal development in Monopterus albus","volume":"39","author":"Wu","year":"2016","journal-title":"J. Hefei Univ. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Fan, M., Yang, W., Zhang, W., and Zhang, L. (2022). The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus. BMC Zool., 7.","DOI":"10.1186\/s40850-022-00155-4"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s00427-005-0477-x","article-title":"Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization","volume":"215","author":"Kondo","year":"2005","journal-title":"Dev. Genes Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1006\/dbio.2000.0129","article-title":"Two sox9 genes on duplicated zebrafish chromosomes: Expression of similar transcription activators in distinct sites","volume":"231","author":"Chiang","year":"2001","journal-title":"Dev. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Wei, L., Yang, C., Tao, W.J., and Wang, D.S. (2016). Genome-Wide Identification and Transcriptome-Based Expression Profiling of the Sox Gene Family in the Nile Tilapia (Oreochromis niloticus). Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030270"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/mrd.10271","article-title":"Similar gene structure of two Sox9a genes and their expression patterns during gonadal differentiation in a teleost fish, rice field eel (Monopterus albus)","volume":"66","author":"Zhou","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"ref_55","unstructured":"Li, X.Y. (2019). Functional Studies of Sox9 Transcription Factor in Gonad and Cartilage Development of Nile Tilapia (Oreochromis niloticus). [Master\u2019s Thesis, Southwest University]."},{"key":"ref_56","unstructured":"Ma, L.M. (2014). Studies on Sex-Related Genes Sox3, Sox9 and Dmrt1 in Sebastes schlegeli. [Ph.D. Thesis, Ocean University of China]."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/jez.361","article-title":"Differential expression and dynamic changes of SOX3 during gametogenesis and sex reversal in protogynous hermaphroditic fish","volume":"307","author":"Yao","year":"2007","journal-title":"J. Exp. Zool. A Ecol. Genet. Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1007\/s10695-022-01110-3","article-title":"Characterization of the transcription factor Sox3 regulating the gonadal development of pearlscale angelfish (Centropyge vrolikii)","volume":"48","author":"Feng","year":"2022","journal-title":"Fish Physiol. Biochem."},{"key":"ref_59","unstructured":"Li, Y.Q. (2021). Studies on the Function and Mechanism of Action of the Transcription Factor Sox3 in the Oogenesis of Nile Tilapia (Oreochromis niloticus). [Master\u2019s Thesis, Southwest University]."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s13238-018-0603-y","article-title":"Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish","volume":"10","author":"Hong","year":"2019","journal-title":"Protein Cell"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wang, T., Gong, G., Li, Z., Niu, J.S., Du, W.X., Wang, Z.W., Wang, Y., Zhou, L., Zhang, X.J., and Lian, Z.Q. (2024). Genomic anatomy of homozygous XX females and YY males reveals early evolutionary trajectory of sex-determining gene and sex chromosomes in Silurus fishes. Mol. Biol. Evol., 13.","DOI":"10.1093\/molbev\/msae169"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1111\/1755-0998.13338","article-title":"Chromosome-level assembly of southern catfish (Silurus meridionalis) provides insights into visual adaptation to nocturnal and benthic lifestyles","volume":"21","author":"Zheng","year":"2021","journal-title":"Mol. Ecol. Resour."},{"key":"ref_63","first-page":"166","article-title":"Role of TGF-\u03b2 signaling pathway in fish sex determination and differentiation","volume":"44","author":"Long","year":"2020","journal-title":"J. Fish. China"},{"key":"ref_64","unstructured":"Hu, Q. (2015). Cloning of Sex-Reversal-Related Genes and Their Regulatory Mechanisms in the Eel (Monopterus albus). [Ph.D. Thesis, Huazhong Agricultural University]."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Wu, G.C., Li, H.W., Tey, W.G., Lin, C.J., and Chang, C.F. (2017). Expression profile of amh\/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185864"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Wu, G.C., Li, H.W., Luo, J.W., Chen, C., and Chang, C.F. (2015). The potential role of Amh to prevent ectopic female development in testicular tissue of the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod., 92.","DOI":"10.1095\/biolreprod.114.126953"},{"key":"ref_67","unstructured":"Dou, X.F. (2013). Cloning of AMH Gene and Its Expression Analysis in Yellow River Carp and Two Species of Loaches. [Master\u2019s Thesis, Henan Normal University]."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1095\/biolreprod.110.084681","article-title":"The expression of Amh and Amhr2 is associated with the development of gonadal tissue and sex change in the protandrous black porgy, Acanthopagrus schlegeli","volume":"83","author":"Wu","year":"2010","journal-title":"Biol. Reprod."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1093\/biolre\/ioy157","article-title":"Female-to-male sex reversal in orange-spotted grouper (Epinephelus coioides) caused by overexpressing of Amh in vivo","volume":"99","author":"Han","year":"2018","journal-title":"Biol. Reprod."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"9691","DOI":"10.1073\/pnas.0611379104","article-title":"The hotei mutation of medaka in the anti-Mullerian hormone receptor causes the dysregulation of germ cell and sexual development","volume":"104","author":"Morinaga","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_71","first-page":"199","article-title":"Induced Expression of Doublesex-and mab-3-related Transcription Factor-1 (Dmrt-1) mRNA by Testosterone in the Olive Flounder, Paralichthys olivaceus ovary","volume":"20","author":"Jo","year":"2007","journal-title":"Biol. Environ. Sci."},{"key":"ref_72","unstructured":"Sun, Y.Y., Zhang, Q.Q., Qi, J., Wang, Z.G., Chen, Y.J., Li, C.M., and Zhong, Q.W. (2008). Cloning and expression analysis of Cynoglossus semilaevis Dmrt1 gene. J. Wuhan Univ., 221\u2013226."},{"key":"ref_73","unstructured":"Yang, D., and Yu, L.N. (2008). Cloning and expression of Nile tilapia Dmrt1 gene in different tissues. Hubei Agric. Sci., 754\u2013757."},{"key":"ref_74","first-page":"72","article-title":"Progress in the study of genes related to sex determination and differentiation in scleractinian fish","volume":"45","author":"Li","year":"2017","journal-title":"J. Henan Norm. Univ."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.ygcen.2019.03.012","article-title":"Dmrt1 (doublesex and mab-3-related transcription factor 1) expression during gonadal development and spermatogenesis in the Japanese eel","volume":"279","author":"Jeng","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Gan, R., Cai, J., Sun, C., Wang, Z., Yang, W., Meng, F., Zhang, L., and Zhang, W. (2023). Transcription factors Dmrt1a, Foxl2, and Nr5a1a potentially interact to regulate cyp19a1a transcription in ovarian follicles of ricefield eel (Monopterus albus). J. Steroid Biochem. Mol. Biol., 231.","DOI":"10.1016\/j.jsbmb.2023.106310"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.cbpa.2017.02.017","article-title":"Significant association of cyp19a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus","volume":"208","author":"Fan","year":"2017","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Lau, E.S., Zhang, Z., Qin, M., and Ge, W. (2016). Knockout of zebrafish ovarian aaromatase gene (Cyp19a1a) by TALEN and CRISPR\/Cas9 leads to all-male offspring due to failed ovarian differentiation. Sci. Rep., 6.","DOI":"10.1038\/srep37357"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Guo, C., Zhang, K., Li, C., Xing, R., Xu, S., Wang, D., and Wang, X. (2024). Cyp19a1a promotes ovarian maturation through regulating E2 synthesis with estrogen receptor 2a in Pampus argenteus (Euphrasen, 1788). Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031583"},{"key":"ref_80","unstructured":"Fan, Z.F. (2017). Studies on the Epigenetic Modification and Regulation of Cyp19a and Its Transcription Factors in Paralichthys olivaceus. [Ph.D. Thesis, The Institute of Oceanology, Chinese Academy of Sciences]."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"739130","DOI":"10.1016\/j.aquaculture.2022.739130","article-title":"Multi-locus gene editing effectively knocked out Cyp19a1a and foxl2 in Monopterus albus, a hermaphroditic fish","volume":"565","author":"Hou","year":"2023","journal-title":"Aquaculture"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s10695-021-01002-y","article-title":"Two Foxo1 homologues in the orange-spotted grouper Epinephelus coioides: Sequences, expression, and possible involvement in the activation of Cyp19a1a expression in the ovary","volume":"47","author":"Ning","year":"2021","journal-title":"Fish Physiol. Biochem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1530\/JME-15-0251","article-title":"Foxo3b but not Foxo3a activates Cyp19a1a in Epinephelus coioides","volume":"56","author":"Liu","year":"2016","journal-title":"J. Mol. Endocrinol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1002\/mrd.22750","article-title":"Multiple roles of Foxj3 in spermatogenesis: A lesson from Foxj3 conditional knockout mouse models","volume":"83","author":"Ni","year":"2016","journal-title":"Mol. Reprod. Dev."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Jasurda, J.S., Jung, D.O., Froeter, E.D., Schwartz, D.B., Hopkins, T.D., Farris, C.L., McGee, S., Narayan, P., and Ellsworth, B.S. (2014). The forkhead transcription factor, FOXP3: A critical role in male fertility in mice. Biol. Reprod., 90.","DOI":"10.1095\/biolreprod.113.112375"},{"key":"ref_86","unstructured":"Yuan, J. (2013). Transcriptomic Studies and Bioinformatics Analysis of the Fox Family During Gonadal Development in Nile Tilapia (Oreochromis niloticus). [Master\u2019s Thesis, Southwest University]."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1159\/000447611","article-title":"Foxl2 and its relatives are evolutionary conserved players in gonadal sex differentiation","volume":"10","author":"Bertho","year":"2016","journal-title":"Sex. Dev."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.ygcen.2013.08.016","article-title":"Foxl2 and Foxl3 are two ancient paralogs that remain fully functional in teleosts","volume":"194","author":"Crespo","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1677\/jme.1.01566","article-title":"An evolutionary and functional analysis of Foxl2 in rainbow trout gonad differentiation","volume":"33","author":"Baron","year":"2004","journal-title":"J. Mol. Endocrinol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ygcen.2018.12.014","article-title":"Expression profiles of Dmrts and Foxls during gonadal development and sex reversal induced by 17\u03b1-methyltestosterone in the orange-spotted grouper","volume":"274","author":"Lyu","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"4388","DOI":"10.1210\/en.2016-1256","article-title":"Foxl3, a Target of miR-9, stimulates spermatogenesis in spermatogonia during natural sex change in Monopterus albus","volume":"157","author":"Gao","year":"2016","journal-title":"Endocrinology"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1534\/genetics.116.199133","article-title":"Sequential, divergent, and cooperative requirements of Foxl2a and Foxl2b in ovary development and maintenance of zebrafish","volume":"205","author":"Yang","year":"2017","journal-title":"Genetics"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Lu, H.J., Zhang, S., Liu, Q.H., Zhang, L.H., and Zhang, W.M. (2014). Cytoplasmic localization of Lrh-1 down-regulates ovarian follicular Cyp19a1a expression in a teleost, the orange-spotted grouper Epinephelus coioides. Biol. Reprod., 91.","DOI":"10.1095\/biolreprod.114.117952"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.tcb.2004.03.008","article-title":"LRH-1: An orphan nuclear receptor involved in development, metabolism and steroidogenesis","volume":"14","author":"Fayard","year":"2004","journal-title":"Trends. Cell Biol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"113871","DOI":"10.1016\/j.ygcen.2021.113871","article-title":"Nr5a homologues in the ricefield eel Monopterus albus: Alternative splicing, tissue-specific expression, and differential roles on the activation of Cyp19a1a promoter in vitro","volume":"312","author":"Yan","year":"2021","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.mce.2017.07.008","article-title":"A homologue of Nr5a1 activates Cyp19a1a transcription additively with Nr5a2 in ovarian follicular cells of the orange-spotted grouper","volume":"460","author":"Shi","year":"2018","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1159\/000381575","article-title":"LRH-1 may rescue SF-1 deficiency for steroidogenesis: An in vitro and in vivo study","volume":"9","author":"Camats","year":"2015","journal-title":"Sex. Dev."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"4157","DOI":"10.1038\/ncomms5157","article-title":"Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena","volume":"5","author":"Takehana","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cub.2012.05.045","article-title":"An immunerelated gene evolved into the master sex-determining gene in rainbow trout, Oncorhynchus mykiss","volume":"22","author":"Yano","year":"2012","journal-title":"Curr. Biol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.cell.2015.10.071","article-title":"Insights into sex chromosome evolution and aging from the genome of a short-lived fish","volume":"163","author":"Reichwald","year":"2015","journal-title":"Cell"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Nakamoto, M., Uchino, T., Koshimizu, E., Kuchiishi, Y., Sekiguchi, R., Wang, L., Sudo, R., Endo, M., Guiguen, Y., and Schartl, M. (2021). A Y-linked anti-M\u00fcllerian hormone type-II receptor is the sex-determining gene in ayu, Plecoglossus altivelis. PLoS Genet., 17.","DOI":"10.1371\/journal.pgen.1009705"},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Kirubakaran, T.G., Andersen, \u00d8., De-Rosa, M.C., Andersstuen, T., Hallan, K., Kent, M.P., and Lien, S. (2019). Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod. Sci. Rep., 9.","DOI":"10.1038\/s41598-018-36748-8"},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Adolfi, M.C., Du, K., Kneitz, S., Cabau, C., Zahm, M., Klopp, C., Feron, R., Paix\u00e3o, R.V., Varela, E.S., and de-Almeida, F.L. (2021). A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas). Sci. Rep., 11.","DOI":"10.1038\/s41598-021-01066-z"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"24359","DOI":"10.1073\/pnas.2009925117","article-title":"Reconstruction of the birth of a male sex chromosome present in Atlantic herring","volume":"117","author":"Rafati","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_105","unstructured":"Wu, X. (2021). Gonadal Transcriptome Analysis and Identification of Candidate Genes for Female Sex Determination in Oreochromis aureus. [Master\u2019s Thesis, Southwest University]."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1016\/j.ygeno.2021.04.007","article-title":"A genome-wide association study, supported by a new chromosome-level genome assembly, suggests Sox2 as a main driver of the undifferentiatiated ZZ\/ZW sex determination of turbot (Scophthalmus maximus)","volume":"113","author":"Robledo","year":"2021","journal-title":"Genomics"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/s41597-019-0139-x","article-title":"Chromosome assembly of Collichthys lucidus, a fish of Sciaenidae with a multiple sex chromosome system","volume":"6","author":"Cai","year":"2019","journal-title":"Sci. Data"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"736622","DOI":"10.1016\/j.aquaculture.2021.736622","article-title":"Production of YY males through self-fertilization of an occasional hermaphrodite in Lanzhou catfish (Silurus lanzhouensis)","volume":"539","author":"Wang","year":"2021","journal-title":"Aquaculture"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/dbio.2000.9883","article-title":"Phylogeny of the SOX family of developmental transcription factors based on sequence and structural indicators","volume":"227","author":"Bowles","year":"2000","journal-title":"Dev. Biol."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/348448a0","article-title":"Genetic evidence equating SRY and the testis-determining factor","volume":"348","author":"Berta","year":"1990","journal-title":"Nature"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1002\/(SICI)1097-010X(19980801)281:5<472::AID-JEZ12>3.0.CO;2-B","article-title":"Evolution of the mammalian Y chromosome and sex-determining gene","volume":"281","author":"Graves","year":"1998","journal-title":"J. Exp. Zool."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.cbpb.2009.05.013","article-title":"Quantitative mRNA expression of Sox3 and Dmrt1 during sex reversal, and expression profiles after GnRHa administration in black porgy, Acanthopagrus schlegeli","volume":"154","author":"Shin","year":"2009","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.jsbmb.2016.01.003","article-title":"Sox3 binds to 11\u03b2-hydroxysteroid dehydrogenase gene promoter suggesting transcriptional interaction in catfish","volume":"158","author":"Rajakumar","year":"2016","journal-title":"J. Steroid. Biochem. Mol. Biol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1210\/mend.15.5.0639","article-title":"Evolution and classification of cystine knot-containing hormones and related extracellular signaling molecules","volume":"15","author":"Vitt","year":"2001","journal-title":"Mol. Endocrinol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/nrm3434","article-title":"TGF-\u03b2 signalling in context","volume":"13","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_116","first-page":"94","article-title":"Characterization of anti-M\u00fcllerian tubular hormone type II receptor gene (Amhr2) expression and preliminary investigation of its function in Paralichthys olivaceus","volume":"43","author":"Li","year":"2024","journal-title":"J. Trop. Oceanogr."},{"key":"ref_117","first-page":"2222","article-title":"Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination","volume":"144","author":"Ge","year":"2017","journal-title":"Development"},{"key":"ref_118","unstructured":"Li, Y.Z. (2023). Expression Studies of Nanos2 in the Gonadal Development of the Eel (Monopterus albus). [Master\u2019s Thesis, Huazhong Agricultural University]."},{"key":"ref_119","first-page":"10","article-title":"Progress in the study of fish sex determination mechanism and related genes","volume":"26","author":"Li","year":"2011","journal-title":"J. Mod. Fish. Inf."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Wu, G.C., Chiu, P.C., Lin, C.J., Lyu, Y.S., Lan, D.S., and Chang, C.F. (2012). Testicular Dmrt1 is involved in the sexual fate of the ovotestis in the protandrous black porgy. Biol. Reprod., 86.","DOI":"10.1095\/biolreprod.111.095695"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s10126-022-10111-5","article-title":"Liposome-encapsulated rec8 and Dmrt1 plasmids induce red-spotted grouper (Epinephelus akaara) testis maturation","volume":"24","author":"Duan","year":"2022","journal-title":"Mar. Biotechnol."},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"Chen, L., Huang, Y., Pan, Q.H., Wang, M.Y., Liang, J.J., and Chen, T.S. (2024). The chinese medaka (Oryzias sinensis) dmrt1 gene converts females to males in medaka (Oryzias latipes). Biochim. Biophys. Acta Gen. Subj., 1868.","DOI":"10.1016\/j.bbagen.2024.130664"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1210\/en.2009-0999","article-title":"Doublesex- and Mab-3-related transcription factor-1 repression of aromatase transcription, a possible mechanism favoring the male pathway in Tilapia","volume":"151","author":"Wang","year":"2010","journal-title":"Endocrinology"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.cbpa.2012.03.021","article-title":"Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus","volume":"162","author":"Cao","year":"2012","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1210\/en.2012-2220","article-title":"Epigenetic modifications during sex change repress gonadotropin stimulation of Cyp19a1a in a teleost ricefield eel (Monopterus albus)","volume":"154","author":"Zhang","year":"2013","journal-title":"Endocrinology"},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Navarro-Mart\u00edn, L., Vi\u00f1as, J., Ribas, L., D\u00edaz, N., Guti\u00e9rrez, A., Di-Croce, L., and Piferrer, F. (2011). DNA methylation of the gonadal aromatase (Cyp19a) promoter is involved in temperature-dependent sex ratio shifts in the European sea bass. PLoS Genet., 7.","DOI":"10.1371\/journal.pgen.1002447"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ygcen.2008.05.016","article-title":"Tissue and sexually dimorphic expression of ovarian and brain aromatase mRNA in the Japanese medaka (Oryzias latipes): Implications for their preferential roles in ovarian and neural differentiation and development","volume":"158","author":"Patil","year":"2008","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"748","DOI":"10.3724\/SP.J.1035.2009.40748","article-title":"Cloning and expressional analysis of Cyp19b gene fragment in Gobiocypris rarus","volume":"33","author":"Cao","year":"2009","journal-title":"Acta. Hydrobiol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1002\/dvdy.21344","article-title":"The zebrafish, brain-specific, aromatase Cyp19a2 is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation","volume":"236","author":"Kallivretaki","year":"2007","journal-title":"Dev. Dyn."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.ygcen.2008.08.009","article-title":"Expression of the two cytochrome P450 aromatase genes in the male and female blue gourami (Trichogaster trichopterus) during the reproductive cycle","volume":"159","author":"Ezagouri","year":"2008","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s10695-023-01291-5","article-title":"Expression characteristics of the Cyp19a1b aromatase gene and its response to 17\u03b2-estradiol treatment in largemouth bass (Micropterus salmoides)","volume":"50","author":"Zhang","year":"2024","journal-title":"Fish Physiol. Biochem."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s00441-022-03629-6","article-title":"Pituitary luteinizing hormone synthesis starts in aromatase (Cyp19a1b)-positive cells expressing esr1 and esr2b at the onset of puberty in Takifugu rubripes (fugu)","volume":"389","author":"Yamaguchi","year":"2022","journal-title":"Cell Tissue Res."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1007\/s10695-023-01270-w","article-title":"Kisspeptin modulation of nonapeptide and cytochrome P450 aromatase mRNA expression in the brain and ovary of the catfish Heteropneustes fossilis: In vivo and in vitro studies","volume":"49","author":"Chaube","year":"2023","journal-title":"Fish Physiol. Biochem."},{"key":"ref_134","unstructured":"Dai, S.F. (2021). Functional Studies of Dmrt1 and Foxl3 in Sex Differentiation and Germ Cell Fate Determination in Nile Tilapia (Oreochromis niloticus). [Ph.D. Thesis, Southwest University]."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1210\/me.2006-0248","article-title":"Foxl2 up-regulates aromatase gene transcription in a female-specific manner by binding to the promoter as well as interacting with ad4 binding protein\/steroidogenic factor 1","volume":"21","author":"Wang","year":"2007","journal-title":"Mol. Endocrinol."},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Wu, G.C., Tey, W.G., Li, H.W., and Chang, C.F. (2015). Sexual fate reprogramming in the steroid-induced bi-directional sex change in the protogynous orange-spotted grouper, Epinephelus coioides. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0145438"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.bbrc.2004.05.133","article-title":"Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus","volume":"320","author":"Wang","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_138","first-page":"96","article-title":"Progress of Cyp19a1 gene in fish sex differentiation and development","volume":"47","author":"Ma","year":"2019","journal-title":"J. Henan Norm. Univ."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ygcen.2013.06.010","article-title":"Isolation and characterization of Ff1 and Gsdf family genes in european sea bass and identification of early gonadal markers of precocious puberty in males","volume":"191","author":"Crespo","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1152\/physrev.00019.2018","article-title":"The orphan nuclear receptors steroidogenic factor-1 and liver receptor homolog-1: Structure, regulation, and essential roles in mammalian reproduction","volume":"99","author":"Meinsohn","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Ji, X., Bu, S., Zhu, Y., Wang, Y., Wen, X., Song, F., and Luo, J. (2022). Identification of SF-1 and Foxl2 and their effect on activating p450 aromatase transcription via specific binding to the promoter motifs in sex reversing Cheilinus undulatus. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.863360"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1210\/en.2015-2049","article-title":"Haploinsufficiency of SF-1 causes female to male sex reversal in nile tilapia, Oreochromis niloticus","volume":"157","author":"Xie","year":"2016","journal-title":"Endocrinology"},{"key":"ref_143","first-page":"387","article-title":"Sexual transformation of unspawned female eels (Monopterus albus)","volume":"45","author":"Fan","year":"2021","journal-title":"J. Aquat. Biol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/2\/119\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:24:23Z","timestamp":1759919063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/2\/119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,7]]},"references-count":143,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ani15020119"],"URL":"https:\/\/doi.org\/10.3390\/ani15020119","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,7]]}}}