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In this study, a xylanase (CbXyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of C. perfringens, were analyzed for their ability to alleviate C. perfringens-induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no C. perfringens challenge; Cp, no enzyme, C. perfringens challenge; Xyn, CbXyn10C plus C. perfringens challenge; Xyn+Am, CbXyn10C+Amylase plus C. perfringens challenge; Xyn+Ap, CbXyn10C+Alkaline protease plus C. perfringens challenge). The C. perfringens CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with C. perfringens (p &lt; 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of Lactobacillus and Butyricicoccus bacteria in the intestine. These results indicated that CbXyn10C and protease can be used to alleviate intestinal damage caused by C. perfringens infection.<\/jats:p>","DOI":"10.3390\/ani15020123","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T07:42:19Z","timestamp":1736235739000},"page":"123","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Alleviating Clostridium perfringens-Induced Intestinal Lesions in Chickens Using the Xylanase CbXyn10C and Its Binary Cocktail with a Protease"],"prefix":"10.3390","volume":"15","author":[{"given":"Wenjing","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daoxin","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wangli","family":"Ji","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kairui","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianshuang","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiying","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2689-8720","authenticated-orcid":false,"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huoqing","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of 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