{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:22Z","timestamp":1787027482550,"version":"build-2736575974"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, FUNCAP","award":["09564039\/2022"],"award-info":[{"award-number":["09564039\/2022"]}]},{"name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, FUNCAP","award":["FPD-0213-00067.01.00\/23"],"award-info":[{"award-number":["FPD-0213-00067.01.00\/23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Guanidinoacetic acid (GAA), a precursor of creatine, has a recognized effect on ruminant performance when used as a dietary supplement. However, its impact on reproductive response remains to be elucidated. Therefore, this study aimed to contribute initially to this area by supplementing the diets of ewes with a high dose of GAA, evaluating its effects on reproductive response. Twenty adult sheep had their estrus synchronized using an MPA sponge, eCG, and PGF2\u03b1. After estrus detection ewes were mated. For 10 days until mating, ewes were grouped in groups of baseline diet (BSD; n = 10) and GAA diet (GAAD; n = 10), which was the BSD with daily 0.9 g\/kg DM of GAA. After the eCG + PGF2\u03b1 dose, the GAAD group exhibited an increase in the peak diastolic and pulsatility of the ovarian artery, a reduction in the systolic\/diastolic peaks ratio, and a larger intraovarian blood perfusion area. A greater depletion of follicles with &lt;3 mm was observed in the GAAD group and a higher number of follicles \u22653 mm. No differences were observed between the diets respect to pregnancy, and twin rates. Thus, a high GAA supply before mating significantly alters ovarian vasculature and improves follicular growth in ewes but does not affect the pregnancy rate.<\/jats:p>","DOI":"10.3390\/ani15020143","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T05:17:08Z","timestamp":1736399828000},"page":"143","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Short Supply of High Levels of Guanidine Acetic Acid, Alters Ovarian Artery Flow and Improves Intraovarian Blood Perfusion Area Associated with Follicular Growth in Sheep"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5603-8042","authenticated-orcid":false,"given":"Marta da Costa","family":"Sousa","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7000-278X","authenticated-orcid":false,"given":"Camila Muniz","family":"Cavalcanti","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8536-5302","authenticated-orcid":false,"given":"Alfredo Jos\u00e9 Herrera","family":"Conde","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9197-1430","authenticated-orcid":false,"given":"Bruna Vit\u00f3ria de Freitas","family":"Alves","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0209-1751","authenticated-orcid":false,"given":"Larissa Fernandes Baia","family":"Cesar","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0600-3582","authenticated-orcid":false,"given":"Jhennyfe Nobre","family":"de Sena","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0992-9871","authenticated-orcid":false,"given":"Yohana Huicho","family":"Miguel","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6205-7086","authenticated-orcid":false,"given":"C\u00e9sar Carneiro Linhares","family":"Fernandes","sequence":"additional","affiliation":[{"name":"School of Health Sciences, University of Fortaleza, Fortaleza 60811-905, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4536-0037","authenticated-orcid":false,"given":"Juliana Paula Martins","family":"Alves","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1799-1675","authenticated-orcid":false,"given":"D\u00e1rcio \u00cdtalo Alves","family":"Teixeira","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0474-8895","authenticated-orcid":false,"given":"Davide","family":"Rondina","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Cear\u00e1 State University (UECE), Fortaleza 60714-903, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-3-030-54462-1_5","article-title":"Amino acids in the nutrition and production of sheep and goats","volume":"1285","author":"Cao","year":"2021","journal-title":"Amino Acids Nutr. 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