{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T18:17:27Z","timestamp":1767205047134,"version":"build-2238731810"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31760728"],"award-info":[{"award-number":["31760728"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2019GXNSFAA245013"],"award-info":[{"award-number":["2019GXNSFAA245013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["AA17204057"],"award-info":[{"award-number":["AA17204057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guangxi Natural Science Foundation","award":["31760728"],"award-info":[{"award-number":["31760728"]}]},{"name":"Guangxi Natural Science Foundation","award":["2019GXNSFAA245013"],"award-info":[{"award-number":["2019GXNSFAA245013"]}]},{"name":"Guangxi Natural Science Foundation","award":["AA17204057"],"award-info":[{"award-number":["AA17204057"]}]},{"name":"Guangxi Key Technologies Research and Development Program","award":["31760728"],"award-info":[{"award-number":["31760728"]}]},{"name":"Guangxi Key Technologies Research and Development Program","award":["2019GXNSFAA245013"],"award-info":[{"award-number":["2019GXNSFAA245013"]}]},{"name":"Guangxi Key Technologies Research and Development Program","award":["AA17204057"],"award-info":[{"award-number":["AA17204057"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Animals"],"abstract":"<jats:p>Fasciola gigantica can modulate host immune mechanisms through excretory\u2013secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite\u2013host immune interaction. To investigate the immunoregulatory function of Abl in Fasciola gigantica, we cloned and expressed the Fasciola gigantica Abl protein and assessed its effect on specific immune functions of buffalo peripheral blood mononuclear cells (PBMCs). Recombinant F. gigantica Abelson tyrosine protein kinase (rFgAbl) was expressed in Escherichia coli. Western blot analysis was performed to assess the reactivity of anti-rFgAbl antibodies with rFgAbl, serum from F. gigantica-infected buffalo, and excretion and secretion products of F. gigantica. Immunohistochemical analysis was conducted to determine the localization of FgAbl in tissues from larval stages and adult worms of F. gigantica. Furthermore, immunofluorescence analysis was utilized to evaluate the binding ability of the rFgAbl protein to buffalo peripheral blood mononuclear cells (PBMCs), as well as to investigate the effects of varying concentrations of rFgAbl protein (5, 10, 20, 40, and 80 \u03bcg\/mL) on the functional responses of PBMCs. Anti-rFgAbl antibodies specifically recognize rFgAbl, serum from buffalo infected with F. gigantica, and FgESP. rFgAbl is localized in the cecum and capsule of juvenile worms, as well as in the testis and viellaria of adult worms. Additionally, rFgAbl enhances cell proliferation, migration, nitric oxide (NO) production, and phagocytosis, while also increasing the transcription levels of cytokines (IFN-\u03b3, IL-12, TNF-\u03b1, IL-4, IL-10, and TGF-\u03b2). The results indicate that rFgAbl can influence the immune function of PBMCs. Further investigation into the immunomodulatory properties of the rFgAbl protein will enhance our understanding of the immune interaction mechanisms between trematodes and their hosts.<\/jats:p>","DOI":"10.3390\/ani15020179","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T11:24:57Z","timestamp":1736508297000},"page":"179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fasciola gigantica Recombinant Abelson Tyrosine Protein Kinase (rFgAbl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells"],"prefix":"10.3390","volume":"15","author":[{"given":"Min","family":"Zhao","sequence":"first","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zou","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanting","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongqi","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengjun","family":"Xian","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiacheng","family":"Tan","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1037-0286","authenticated-orcid":false,"given":"Wenda","family":"Di","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wende","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6185-7505","authenticated-orcid":false,"given":"Dongying","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.vetpar.2016.03.011","article-title":"Fasciola hepatica: A light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy","volume":"221","author":"Hanna","year":"2016","journal-title":"Vet. 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