{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T11:13:26Z","timestamp":1759922006758,"version":"build-2065373602"},"reference-count":83,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Pain management requires the identification of certain indicators to recognize pain. Various tools have been suggested to achieve an objective evaluation, including infrared thermography (IRT). The objective of this study was to assess the facial thermal nociceptive response produced by the use of cannabidiol (CBD) alone and in combination with meloxicam in female dogs undergoing elective ovariohysterectomy anesthetized with isoflurane. Sixty-four female dogs of different breeds were randomly distributed into four study groups according to the treatment received. G1: Placebo group (n = 16); G2: Group receiving intravenous meloxicam as premedication (0.2 mg Kg\u22121) and every 24 h postoperatively 0.1 mg Kg\u22121 (n = 16); G3: Group treated with CBD (n = 16) at a dose of 2 mg kg\u22121 orally every 12 h; and G4: Group medicated with the combination of both treatments (n = 16). All treatments were administered for 48 h postoperatively. After the anesthetic surgical procedure, radiometric images were captured using IRT and physiological parameters during the events EBasal, E30min, E1h, E2h, E3h, E4h, E8h, E12h, E24h and E48h. Overall, it was found that the high, medium and low temperatures of the thermal windows of the eye, upper eyelid and lower eyelid, as well as the average temperature of the lacrimal gland in G1 between events, were significantly lower at E30min, E1h and E2h compared to EBasal (p = 0.01). Among treatments, a significantly higher temperature was observed in groups G2, G3 and G4 compared to G1 (p = 0.001) in the thermal windows of the upper eyelid, lower eyelid, lacrimal gland and ocular areas. Regarding physiological parameters, heart rate (HR) was higher in G1 compared to the animals in G2, G3 and G4 (p = 0.03). The respiratory rate (RR) was significantly lower in all four study groups during the postoperative events compared to their respective EBasal (p &lt; 0.05), while among treatments, G2, G3 and G4 had a lower RR compared to G1 (p = 0.03). Mild hypothermia was observed in all study groups at E30min and E1h compared to EBasal (p = 0.001). No significant correlation was found between the temperatures of the assessed thermal regions and the physiological traits. In conclusion, CBD, whether administered alone or in combination with meloxicam, demonstrated comparable analgesic efficacy, which could control nociceptive cardiorespiratory and hemodynamic autonomic responses, as there were no significant changes in the facial thermal response between treatments G2, G3 and G4.<\/jats:p>","DOI":"10.3390\/ani15020227","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T11:32:29Z","timestamp":1736940749000},"page":"227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessing Facial Thermal Nociceptive Response in Female Dogs After Elective Ovariohysterectomy Anesthetized with Isoflurane and Treated with Cannabidiol and Meloxicam Analgesia"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8382-8388","authenticated-orcid":false,"given":"Alejandro","family":"Casas-Alvarado","sequence":"first","affiliation":[{"name":"PhD Program in Biological and Health Sciences [Doctorado en Ciencias Biol\u00f3gicas y de la Salud], Universidad Aut\u00f3noma Metropolitana (UAM), Mexico City 04960, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8153-8210","authenticated-orcid":false,"given":"Patricia","family":"Mora-Medina","sequence":"additional","affiliation":[{"name":"Facultad de Estudios Superiores Cuautitl\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Cuautitl\u00e1n 54714, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4271-2906","authenticated-orcid":false,"given":"Ismael","family":"Hern\u00e1ndez-Avalos","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Veterinary Anesthesia, Biological Sciences Department, Facultad de Estudios Superiores Cuautitl\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Cuautitl\u00e1n 54714, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-4261","authenticated-orcid":false,"given":"Julio","family":"Mart\u00ednez-Burnes","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Instituto de Ecolog\u00eda Aplicada, Universidad Aut\u00f3noma de Tamaulipas, Victoria City 87000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0052-861X","authenticated-orcid":false,"given":"Agatha","family":"Miranda-Cortes","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Veterinary Anesthesia, Biological Sciences Department, Facultad de Estudios Superiores Cuautitl\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Cuautitl\u00e1n 54714, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1584-0654","authenticated-orcid":false,"given":"Adriana","family":"Dom\u00ednguez-Oliva","sequence":"additional","affiliation":[{"name":"Neurophysiology of Pain, Behavior and Assessment of Welfare in Domestic Animals, Department of Animal Production and Agriculture, Universidad Aut\u00f3noma Metropolitana (UAM), Mexico City 04960, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0562-0367","authenticated-orcid":false,"given":"Daniel","family":"Mota-Rojas","sequence":"additional","affiliation":[{"name":"Neurophysiology of Pain, Behavior and Assessment of Welfare in Domestic Animals, Department of Animal Production and Agriculture, Universidad Aut\u00f3noma Metropolitana (UAM), Mexico City 04960, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,15]]},"reference":[{"key":"ref_1","first-page":"2984","article-title":"Nociceptive pain and axiety in equines: Physiological and behavioral alterations","volume":"14","year":"2021","journal-title":"Vet. World"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.rvsc.2017.05.009","article-title":"Evaluation of the Parasympathetic Tone Activity (PTA) index to assess the analgesia\/nociception balance in anaesthetised dogs","volume":"115","author":"Mansour","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/jsap.13566","article-title":"2022 WSAVA guidelines for the recognition, assessment and treatment of pain","volume":"64","author":"Monteiro","year":"2023","journal-title":"J. Small Anim. Pract."},{"key":"ref_4","unstructured":"Orihuela, A., and Ungerfeld, R. (2020). Pr\u00e1cticas Zoot\u00e9cnicas Dolorosas; Evaluaci\u00f3n y Alternativas para el Bienestar Animal, Editorial Colegio de Postgraduados; Fundaci\u00f3n COLPOS. [1st ed.]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/23144599.2019.1680044","article-title":"Review of different methods used for clinical recognition and assessment of pain in dogs and cats","volume":"7","year":"2019","journal-title":"Int. J. Vet. Sci. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1467-2987.2004.00175.x","article-title":"Attitudes and concerns of French veterinarians towards pain and analgesia in dogs and cats","volume":"31","author":"Hugonnard","year":"2004","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.rvsc.2019.03.002","article-title":"Perception, knowledge and attitudes of small animal practitioners regarding animal abuse and interpersonal violence in Brazil and Colombia","volume":"124","author":"Monsalve","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Casas-Alvarado, A., Mota-Rojas, D., Hern\u00e1ndez-\u00c1valos, I., Mora-Medina, P., Olmos-Hern\u00e1ndez, A., Verduzco-Mendoza, A., Reyes-Sotelo, B., and Mart\u00ednez-Burnes, J. (2020). Advances in infrared thermography: Surgical aspects, vascular changes, and pain monitoring in veterinary medicine. J. Therm. Biol., 92.","DOI":"10.1016\/j.jtherbio.2020.102664"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Whittaker, A.L., Muns, R., Wang, D., Mart\u00ednez-Burnes, J., Hern\u00e1ndez-\u00c1valos, I., Casas-Alvarado, A., Dom\u00ednguez-Oliva, A., and Mota-Rojas, D. (2023). Assessment of Pain and Inflammation in Domestic Animals Using Infrared Thermography: A Narrative Review. Animals, 13.","DOI":"10.3390\/ani13132065"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Gonz\u00e1lez, D., Guerrero Legarreta, I., Cruz-Monterrosa, R.G., Napolitano, F., Titto, C.G., Abd El-Aziz, A.H., Hern\u00e1ndez-Avalos, I., Casas-Alvarado, A., Dom\u00ednguez-Oliva, A., and Mota-Rojas, D. (2023). Assessment of thermal changes in water buffalo mobilized from the paddock and transported by short journeys. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1184577"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Casas-Alvarado, A., Mart\u00ednez-Burnes, J., Mora-Medina, P., Hern\u00e1ndez-Avalos, I., Dom\u00ednguez-Oliva, A., Lezama-Garc\u00eda, K., G\u00f3mez-Prado, J., and Mota-Rojas, D. (2022). Thermal and Circulatory Changes in Diverse Body Regions in Dogs and Cats Evaluated by Infrared Thermography. Animals, 12.","DOI":"10.3390\/ani12060789"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Casas-Alvarado, A., Mota-Rojas, D., Hern\u00e1ndez-\u00c1valos, I., Mart\u00ednez-Burnes, J., Rosas, M.E., Miranda-Cort\u00e9s, A., Dom\u00ednguez-Oliva, A., and Mora-Medina, P. (2023). Assessment of thermal response, cardiorespiratory parameters and post-operative analgesia in dogs undergoing ovariohysterectomy with different combinations of epidural analgesia and isoflurane. J. Anim. Behav. Biometeorol., 11.","DOI":"10.31893\/jabb.23009"},{"key":"ref_13","first-page":"1","article-title":"Evaluation of the averaged parasympathetic tone activity and its dynamic variation to assess intraoperative nociception in relation to hemodynamic changes in dogs","volume":"43","author":"Morgaz","year":"2023","journal-title":"Vet. Q."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.vaa.2019.09.006","article-title":"Intraoperative nociception-antinociception monitors: A review from the veterinary perspective","volume":"47","author":"Granados","year":"2020","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_15","first-page":"1","article-title":"Clinical usefulness of infrared thermography to detect sick animals: Frequent and current cases","volume":"2022","author":"Pereira","year":"2022","journal-title":"CABI Rev."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lezama-Garc\u00eda, K., Mart\u00ednez-Burnes, J., Marcet-Rius, M., Gazzano, A., Olmos-Hern\u00e1ndez, A., Mora-Medina, P., Dom\u00ednguez-Oliva, A., Pereira, A.M.F., Hern\u00e1ndez-\u00c1valos, I., and Baqueiro-Espinosa, U. (2022). Is the Weight of the Newborn Puppy Related to Its Thermal Balance?. Animals, 12.","DOI":"10.3390\/ani12243536"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.physbeh.2007.11.044","article-title":"Eye temperature and heart rate variability of calves disbudded with or without local anaesthetic","volume":"93","author":"Stewart","year":"2008","journal-title":"Physiol. Behav."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.physbeh.2007.04.034","article-title":"Non-invasive measurement of stress in dairy cows using infrared thermography","volume":"92","author":"Stewart","year":"2007","journal-title":"Physiol. Behav."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sutherland, M.A., Worth, G.M., Dowling, S.K., Lowe, G.L., Cave, V.M., and Stewart, M. (2020). Evaluation of infrared thermography as a non-invasive method of measuring the autonomic nervous response in sheep. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0233558"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Redaelli, V., Papa, S., Marsella, G., Grignaschi, G., Bosi, A., Ludwig, N., Luzi, F., Vismara, I., Rimondo, S., and Veglianese, P. (2019). A refinement approach in a mouse model of rehabilitation research. Analgesia strategy, reduction approach and infrared thermography in spinal cord injury. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0224337"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Miranda-Cort\u00e9s, A., Mota-Rojas, D., Crosignani-Outeda, N., Casas-Alvarado, A., Mart\u00ednez-Burnes, J., Olmos-Hern\u00e1ndez, A., Mora-Medina, P., Verduzco-Mendoza, A., and Hern\u00e1ndez-\u00c1valos, I. (2023). The role of cannabinoids in pain modulation in companion animals. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.1050884"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Gamble, L.-J., Boesch, J.M., Frye, C.W., Schwark, W.S., Mann, S., Wolfe, L., Brown, H., Berthelsen, E.S., and Wakshlag, J.J. (2018). Pharmacokinetics, Safety, and Clinical Efficacy of Cannabidiol Treatment in Osteoarthritic Dogs. Front. Vet. Sci., 5.","DOI":"10.3389\/fvets.2018.00165"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0022-3565(24)29345-9","article-title":"Mechanisms of endocannabinoid inactivation: Biochemistry and pharmacology","volume":"298","author":"Giuffrida","year":"2001","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1089\/can.2017.0017","article-title":"Cannabis and Pain: A Clinical Review","volume":"2","author":"Hill","year":"2017","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Starowicz, K., and Finn, D.P. (2017). Cannabinoids and Pain: Sites and Mechanisms of Action, Elsevier Inc.. [1st ed.].","DOI":"10.1016\/bs.apha.2017.05.003"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Interlandi, C., Tabb\u00ec, M., Di Pietro, S., D\u2019Angelo, F., Costa, G.L., Arfuso, F., Giudice, E., Licata, P., Macr\u00ec, D., and Crupi, R. (2024). Improved quality of life and pain relief in mature horses with osteoarthritis after oral transmucosal cannabidiol oil administration as part of an analgesic regimen. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1341396"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"366","DOI":"10.5187\/jast.2019.61.6.366","article-title":"A simple method to evaluate body condition score to maintain the optimal body weight in dogs","volume":"61","author":"Chun","year":"2019","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4103\/0253-7176.116232","article-title":"How to calculate sample size for different study designs in medical research?","volume":"35","author":"Charan","year":"2013","journal-title":"Indian J. Psychol. Med."},{"key":"ref_29","unstructured":"Doyle, D.J., Goyal, A., Bansal, P., and Garmon, E.H. (2020). American Society of Anesthesiologists Classification (ASA Class), StatPearls Publishing."},{"key":"ref_30","first-page":"237","article-title":"Guidelines for safe and effective use of NSAIDs in dogs","volume":"6","author":"Lascelles","year":"2005","journal-title":"Vet. Ther."},{"key":"ref_31","unstructured":"Chohan, A.S., and Davidow, E.B. (2017). Clinical Pharmacology and Administration of Fluid, Electrolyte, and Blood Component Solutions. Veterinary Anesthesia and Analgesia, John Wiley & Sons, Ltd."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1748-5827.2008.00670.x","article-title":"Does pethidine affect the cardiovascular and sedative effects of dexmedetomidine in dogs?","volume":"50","author":"Grint","year":"2009","journal-title":"J. Small Anim. Pract."},{"key":"ref_33","unstructured":"Traquilli, W.J., Thurmon, J.C., and Grimm, K.A. (2007). Injectable and alternative anesthniques. Lumbs & Jones Veterinary Anesthesia and Analgesia, Blackwell Publishing Ltd."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"404","DOI":"10.5455\/javar.2021.h529","article-title":"Clinical pharmacology of tramadol and tapentadol, and their therapeutic efficacy in different models of acute and chronic pain in dogs and cats","volume":"8","year":"2021","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_35","unstructured":"(2024, October 30). NOM-062-ZOO-1999. Especificaciones T\u00e9cnicas Para la Producci\u00f3n, Cuidado y Uso de los Animales de Laboratorio, Available online: http:\/\/publico.senasica.gob.mx\/?doc=743."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/0976-500X.72351","article-title":"Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research","volume":"1","author":"Kilkenny","year":"2010","journal-title":"J. Pharmacol. Pharmacother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0168-1591(02)00288-5","article-title":"Guidelines for the ethical use of animals in applied ethology studies","volume":"81","author":"Sherwin","year":"2003","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1538\/expanim.20-0052","article-title":"Infrared thermal imaging associated with pain in laboratory animals","volume":"70","author":"Orihuela","year":"2021","journal-title":"Exp. Anim."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mota-Rojas, D., Dom\u00ednguez-Oliva, A., Mart\u00ednez-Burnes, J., Casas-Alvarado, A., and Hern\u00e1ndez-\u00c1valos, I. (2023). Euthanasia and Pain in Canine Patients with Terminal and Chronic-Degenerative Diseases: Ethical and Legal Aspects. Animals, 13.","DOI":"10.3390\/ani13071265"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.3168\/jds.2010-3114","article-title":"Noninvasive assessment of autonomic activity for evaluation of pain in calves, using surgical castration as a model","volume":"93","author":"Stewart","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.tvjl.2004.02.005","article-title":"Dehorning and disbudding distress and its alleviation in calves","volume":"169","author":"Stafford","year":"2005","journal-title":"Vet. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/00480169.2005.36560","article-title":"The welfare significance of the castration of cattle: A review","volume":"53","author":"Stafford","year":"2005","journal-title":"N. Z. Vet. J."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Casas-Alvarado, A., Ogi, A., Villanueva-Garc\u00eda, D., Mart\u00ednez-Burnes, J., Hern\u00e1ndez-Avalos, I., Olmos-Hern\u00e1ndez, A., Mora-Medina, P., Dom\u00ednguez-Oliva, A., and Mota-Rojas, D. (2024). Application of Infrared Thermography in the Rehabilitation of Patients in Veterinary Medicine. Animals, 14.","DOI":"10.3390\/ani14050696"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Mota-Rojas, D., Ghezzi, M.D., Hern\u00e1ndez-\u00c1valos, I., Dom\u00ednguez-Oliva, A., Casas-Alvarado, A., Lendez, P.A., Ceriani, M.C., and Wang, D. (2024). Hypothalamic Neuromodulation of Hypothermia in Domestic Animals. Animals, 14.","DOI":"10.3390\/ani14030513"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0195-5616(08)70003-2","article-title":"Physiology of Pain","volume":"30","author":"Lamont","year":"2000","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.cnc.2017.08.001","article-title":"Physiology of Pain","volume":"29","author":"Ellison","year":"2017","journal-title":"Crit. Care Nurs. Clin. N. Am."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.tvjl.2018.05.004","article-title":"The neurobiology of acute pain","volume":"237","author":"Bell","year":"2018","journal-title":"Vet. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"117","DOI":"10.2478\/jvetres-2021-0004","article-title":"Clinical use of the parasympathetic tone activity index as a measurement of postoperative analgaesia in dogs undergoing ovariohysterectomy","volume":"65","author":"Valverde","year":"2021","journal-title":"J. Vet. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.tvjl.2012.03.001","article-title":"An overview of the physiology of pain for the veterinarian","volume":"193","author":"Meintjes","year":"2012","journal-title":"Vet. J."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Downing, R., and Della Rocca, G. (2023). Pain in Pets: Beyond Physiology. Animals, 13.","DOI":"10.3390\/ani13030355"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/j.1476-5381.2012.02223.x","article-title":"New concepts in pharmacological efficacy at 7TM receptors: IUPHAR Review 2","volume":"168","author":"Kenakin","year":"2013","journal-title":"Br. J. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/bs.mie.2017.05.003","article-title":"Techniques for the Cellular and Subcellular Localization of Endocannabinoid Receptors and Enzymes in the Mammalian Brain","volume":"Volume 593","author":"Cristino","year":"2017","journal-title":"Methods Enzymologic"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1146\/annurev.physiol.010908.163149","article-title":"Endocannabinoid Signaling and Long-Term Synaptic Plasticity","volume":"71","author":"Heifets","year":"2009","journal-title":"Annu. Rev. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Freundt-Revilla, J., Kegler, K., Baumg\u00e4rtner, W., and Tipold, A. (2017). Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181064"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s00418-018-1684-7","article-title":"Localization of cannabinoid receptors CB1, CB2, GPR55, and PPAR\u03b1 in the canine gastrointestinal tract","volume":"150","author":"Galiazzo","year":"2018","journal-title":"Histochem. Cell Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/sj.bjp.0707584","article-title":"CB 2 receptors in the brain: Role in central immune function","volume":"153","author":"Cabral","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Mlost, J., Bryk, M., and Starowicz, K. (2020). Cannabidiol for Pain Treatment: Focus on Pharmacology and Mechanism of Action. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228870"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"507","DOI":"10.2174\/156800705774322111","article-title":"Cannabinoids","volume":"4","author":"Grotenhermen","year":"2005","journal-title":"Curr. Drug Target CNS Neurol. Disord."},{"key":"ref_59","first-page":"14","article-title":"Pharmacology of cannabinoids","volume":"25","author":"Grotenhermen","year":"2005","journal-title":"Neuro Endocrinol. Lett."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Silver, R.J. (2019). The Endocannabinoid System of Animals. Animals, 9.","DOI":"10.20944\/preprints201906.0262.v1"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/sj.bjp.0705667","article-title":"Cannabinoids and neuroinflammation","volume":"141","author":"Walter","year":"2004","journal-title":"Br. J. Pharmacol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1248\/bpb.34.774","article-title":"Evaluation of the Cyclooxygenase Inhibiting Effects of Six Major Cannabinoids Isolated from Cannabis sativa","volume":"34","author":"Ruhaak","year":"2011","journal-title":"Biol. Pharm. Bull."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Gugliandolo, E., Licata, P., Peritore, A.F., Siracusa, R., D\u2019Amico, R., Cordaro, M., Fusco, R., Impellizzeri, D., Di Paola, R., and Cuzzocrea, S. (2021). Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood. Vet. Sci., 8.","DOI":"10.3390\/vetsci8090185"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.cvsm.2008.06.005","article-title":"Multimodal Pain Management in Veterinary Medicine: The Physiologic Basis of Pharmacologic Therapies","volume":"38","author":"Lamont","year":"2008","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_65","first-page":"565","article-title":"A comparison of the analgesic effects of butorphanol with those of meloxicam after elective ovariohysterectomy in dogs","volume":"44","author":"Caulkett","year":"2003","journal-title":"Can. Vet. J. = La Rev. Vet. Can."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0306-4522(00)00361-4","article-title":"Localization and regulation of cyclo-oxygenase-1 and -2 and neuronal nitric oxide synthase in mouse spinal cord","volume":"101","author":"Tegeder","year":"2000","journal-title":"Neuroscience"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1046\/j.1471-4159.2001.00613.x","article-title":"Effects of selective COX-1 and -2 inhibition on formalin-evoked nociceptive behaviour and prostaglandin E2 release in the spinal cord","volume":"79","author":"Tegeder","year":"2008","journal-title":"J. Neurochem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.cvsm.2019.07.009","article-title":"Antiinflammatory Drugs","volume":"49","author":"Monteiro","year":"2019","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1096\/fj.05-5346com","article-title":"Inhibition of prostaglandin E 2 synthesis by SC--560 is independent of cyclooxygenase 1 inhibition","volume":"20","author":"Brenneis","year":"2006","journal-title":"FASEB J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1523\/JNEUROSCI.5054-03.2004","article-title":"Constitutive Spinal Cyclooxygenase-2 Participates in the Initiation of Tissue Injury-Induced Hyperalgesia","volume":"24","author":"Ghilardi","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1111\/j.1399-6576.2012.02730.x","article-title":"Non-steroidal anti-inflammatory drugs reduce the stress response during sevoflurane anesthesia","volume":"56","author":"Liu","year":"2012","journal-title":"Acta Anaesthesiol. Scand."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"393","DOI":"10.14202\/vetworld.2021.393-404","article-title":"Neurobiology of anesthetic-surgical stress and induced behavioral changes in dogs and cats: A review","volume":"14","year":"2021","journal-title":"Vet. World"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Layton, R., Layton, D., Beggs, D., Fisher, A., Mansell, P., and Stanger, K.J. (2023). The impact of stress and anesthesia on animal models of infectious disease. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1086003"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1177\/1098612X13476033","article-title":"Multimodal analgesia for perioperative pain in three cats","volume":"15","author":"Steagall","year":"2013","journal-title":"J. Feline Med. Surg."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Casas-Alvarado, A., Mart\u00ednez-Burnes, J., Hern\u00e1ndez-\u00c1valos, I., Mora-Medina, P., Miranda-Cort\u00e9s, A., Dom\u00ednguez-Oliva, A., and Mota-Rojas, D. (2024). Assessment of the nociceptive response to the use of cannabidiol alone and in combination with meloxicam through infrared pupillometry in female dogs undergoing elective ovariohysterectomy. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1380022"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Johnson, C. (2016). Research tools for the measurement of pain and nociception. Animals, 6.","DOI":"10.3390\/ani6110071"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1080\/23328940.2017.1413155","article-title":"Role of the median preoptic nucleus in the autonomic response to heat-exposure","volume":"5","author":"Abbott","year":"2018","journal-title":"Temperature"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"R824","DOI":"10.1152\/ajpregu.00221.2004","article-title":"Descending vasomotor pathways from the dorsomedial hypothalamic nucleus: Role of medullary raphe and RVLM","volume":"287","author":"Horiuchi","year":"2004","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev-physiol-020518-114546","article-title":"Central Mechanisms for Thermoregulation","volume":"81","author":"Morrison","year":"2019","journal-title":"Annu. Rev. Physiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jveb.2014.11.003","article-title":"Hot dogs: Thermography in the assessment of stress in dogs (Canis familiaris)\u2014A pilot study","volume":"10","author":"Travain","year":"2015","journal-title":"J. Vet. Behav."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.physbeh.2016.03.019","article-title":"How good is this food? A study on dogs\u2019 emotional responses to a potentially pleasant event using infrared thermography","volume":"159","author":"Travain","year":"2016","journal-title":"Physiol. Behav."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2460\/javma.1998.212.01.61","article-title":"Comparison of three methods used for assessment of pain in dogs","volume":"212","author":"Holton","year":"1998","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.tvjl.2018.04.013","article-title":"Measuring pain in dogs and cats using structured behavioural observation","volume":"236","author":"Reid","year":"2018","journal-title":"Vet. 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