{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T19:03:52Z","timestamp":1788894232234,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"projects of the \u201cYunnan Revitalization Talent Support Program\u201d, China","award":["32260841"],"award-info":[{"award-number":["32260841"]}]},{"name":"projects of the \u201cYunnan Revitalization Talent Support Program\u201d, China","award":["U2202203"],"award-info":[{"award-number":["U2202203"]}]},{"name":"projects of the \u201cYunnan Revitalization Talent Support Program\u201d, China","award":["202201AS070063"],"award-info":[{"award-number":["202201AS070063"]}]},{"name":"projects of the \u201cYunnan Revitalization Talent Support Program\u201d, China","award":["2024Y308"],"award-info":[{"award-number":["2024Y308"]}]},{"name":"projects of the \u201cYunnan Revitalization Talent Support Program\u201d, China","award":["FWCY-BSPY2024087"],"award-info":[{"award-number":["FWCY-BSPY2024087"]}]},{"name":"National Natural Science Foundation of China","award":["32260841"],"award-info":[{"award-number":["32260841"]}]},{"name":"National Natural Science Foundation of China","award":["U2202203"],"award-info":[{"award-number":["U2202203"]}]},{"name":"National Natural Science Foundation of China","award":["202201AS070063"],"award-info":[{"award-number":["202201AS070063"]}]},{"name":"National Natural Science Foundation of China","award":["2024Y308"],"award-info":[{"award-number":["2024Y308"]}]},{"name":"National Natural Science Foundation of China","award":["FWCY-BSPY2024087"],"award-info":[{"award-number":["FWCY-BSPY2024087"]}]},{"name":"Key Project of Basic Research Program of Science and Technology Department of Yunnan Province","award":["32260841"],"award-info":[{"award-number":["32260841"]}]},{"name":"Key Project of Basic Research Program of Science and Technology Department of Yunnan Province","award":["U2202203"],"award-info":[{"award-number":["U2202203"]}]},{"name":"Key Project of Basic Research Program of Science and Technology Department of Yunnan Province","award":["202201AS070063"],"award-info":[{"award-number":["202201AS070063"]}]},{"name":"Key Project of Basic Research Program of Science and Technology Department of Yunnan Province","award":["2024Y308"],"award-info":[{"award-number":["2024Y308"]}]},{"name":"Key Project of Basic Research Program of Science and Technology Department of Yunnan Province","award":["FWCY-BSPY2024087"],"award-info":[{"award-number":["FWCY-BSPY2024087"]}]},{"name":"Yunnan Provincial Department of Education project","award":["32260841"],"award-info":[{"award-number":["32260841"]}]},{"name":"Yunnan Provincial Department of Education project","award":["U2202203"],"award-info":[{"award-number":["U2202203"]}]},{"name":"Yunnan Provincial Department of Education project","award":["202201AS070063"],"award-info":[{"award-number":["202201AS070063"]}]},{"name":"Yunnan Provincial Department of Education project","award":["2024Y308"],"award-info":[{"award-number":["2024Y308"]}]},{"name":"Yunnan Provincial Department of Education project","award":["FWCY-BSPY2024087"],"award-info":[{"award-number":["FWCY-BSPY2024087"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 \u00b1 0.61%) and the 10 with the lowest milk fat content (LF, 1.49 \u00b1 0.13%) were selected. Their rumen fluid and plasma were collected for rumen microbiota and metabolome analysis. The results showed that the rumen bacteria abundance of Synergistota, Quinella, Selenomonas, and Fretibacterium was significantly higher in the HF buffaloes. The abundance of 14 rumen fungi, including Candida, Talaromyces, Cyrenella, and Stilbella, was significantly higher in the HF buffaloes. The analysis of the metabolites in the rumen and plasma showed that several metabolites differed between the HF and LF buffaloes. A total of 68 and 42 differential metabolites were identified in the rumen and plasma, respectively. By clustering these differential metabolites, most of those clustered in the HF group were lipid and lipid-like molecules such as secoeremopetasitolide B, lucidenic acid J LysoPE (0:0\/18:2 (9Z, 12Z)), and 5-tetradecenoic acid. Spearman\u2019s rank correlations showed that Quinella, Fretibacterium, Selenomonas, Cyrenella, and Stilbella were significantly positively correlated with the metabolites of the lipids and lipid-like molecules in the rumen and plasma. The results suggest that rumen microbiota such as Quinella, Fretibacterium, Selenomonas, and Cyrenella may regulate milk fat synthesis by influencing the lipid metabolites in the rumen and plasma. In addition, the combined analysis of the rumen microbiota and host metabolites may provide a fundamental understanding of the role of the microbiota and host in regulating milk fat synthesis.<\/jats:p>","DOI":"10.3390\/ani15020248","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T03:33:02Z","timestamp":1737084782000},"page":"248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2517-1282","authenticated-orcid":false,"given":"Ye","family":"Yu","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7491-3354","authenticated-orcid":false,"given":"Runqi","family":"Fu","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunjia","family":"Jin","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Han","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Gao","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binlong","family":"Fu","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Qi","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Yunnan Animal Husbandry Station, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Leng","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"key":"ref_1","first-page":"97","article-title":"The chemical composition of milk from free-ranging African buffalo (Syncerus caffer)","volume":"9","author":"Gernot","year":"2009","journal-title":"S. 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