{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:40:10Z","timestamp":1774690810958,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Formosan serows are an endemic species in Taiwan. Alfaxalone, a \u03b3-aminobutyric acidA agonist, induces or maintains anesthesia in various veterinary species with reported potential adverse effects of respiratory depression and tachycardia. \u03b12-Adrenoceptor agonists exert sedative and muscle relaxation effects, along with substantial cardiovascular adverse effects. Here, we aimed to evaluate the anesthetic effects of alfaxalone combined with xylazine or dexmedetomidine (AX vs. AD, respectively) in Formosan serows. In this randomized, masked study, AX was administered to four serows, and AD was administered to five serows intramuscularly via blow dart. The time and score of induction and recovery were recorded. Post-intubation, isoflurane was administered for maintenance anesthesia. Heart rate (HR), respiratory rate (RR), peripheral saturation of oxygenation (SpO2), rectal temperature (RT), and end-tidal CO2 (EtCO2) were recorded every five to eight minutes. Atipamezole and tolazoline were administered to antagonize dexmedetomidine and xylazine post-procedure, respectively. Both combinations allowed smooth induction and recovery. The AD group exhibited significantly lower HR and SpO2 and significantly higher RT and EtCO2 than the AX group (both p &lt; 0.01). The AD-treated serows exhibited notable muscle rigidity after induction and significant hypoventilation and hypoxemia during the procedure. Although alfaxalone combined with dexmedetomidine or xylazine can produce satisfactory induction and recovery in Formosa serows, notable hypoxemia and hypoventilation are induced by the alfaxalone\u2013dexmedetomidine combination compared to the alfaxalone\u2013xylazine combination.<\/jats:p>","DOI":"10.3390\/ani15030307","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T07:55:32Z","timestamp":1737532532000},"page":"307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of the Anesthetic Effects of Alfaxalone Combined with Xylazine or Dexmedetomidine in Captive Formosa Serows (Capricornis swinhoei)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0207-4520","authenticated-orcid":false,"given":"Li-Jen","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Science, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1166-6433","authenticated-orcid":false,"given":"Toshitsugu","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Science, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-Yeh","family":"Lien","sequence":"additional","affiliation":[{"name":"Animal Medical Center, Taipei Zoo, Taipei 11656, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuan-Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Veterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lue, K.Y. (1987). A preliminary study on the ecology of Formosan serow Capricornis crispus swinhoei. The Biology and Management of Capricornis and Related Mountain Antelopes, Springer.","DOI":"10.1007\/978-94-011-8030-6_10"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Moberg, G.P. (2000). Biological response to stress: Implications for animal welfare. The Biology of Animal Stress: Basic Principles and Implications for Animal Welfare, CABI Publishing.","DOI":"10.1079\/9780851993591.0000"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/j.1467-2995.2005.00264.x","article-title":"Plasma pharmacokinetics of alfaxalone in dogs after an intravenous bolus of Alfaxan-CD RTU","volume":"33","author":"Pasloske","year":"2006","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_4","first-page":"55","article-title":"Althesin in the larger animal","volume":"48","author":"Hall","year":"1972","journal-title":"Postgrad. Med. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1476-5381.1987.tb11198.x","article-title":"Modulation of GABAA receptor activity by alphaxalone","volume":"90","author":"Cottrell","year":"1987","journal-title":"Br. J. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1111\/vaa.12300","article-title":"Determination of the minimum infusion rate of alfaxalone during its co-administration with fentanyl at three different doses by constant rate infusion intravenously in goats","volume":"43","author":"Dzikiti","year":"2016","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e000065","DOI":"10.1136\/vetreco-2014-000065","article-title":"Determination of the minimum infusion rate of alfaxalone during its co-administration with midazolam in goats","volume":"2","author":"Dzikiti","year":"2015","journal-title":"Vet. Rec. Open"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4102\/jsava.v85i1.1047","article-title":"The effects of midazolam and butorphanol, administered alone or combined, on the dose and quality of anaesthetic induction with alfaxalone in goats","volume":"85","author":"Dzikiti","year":"2014","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1177\/1098612X17742289","article-title":"Cardiovascular, respiratory and sedative effects of intramuscular alfaxalone, butorphanol and dexmedetomidine compared with ketamine, butorphanol and dexmedetomidine in healthy cats","volume":"20","author":"Cremer","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1177\/1098612X17732484","article-title":"Evaluation of an intramuscular butorphanol and alfaxalone protocol for feline blood donation: A pilot study","volume":"20","author":"Granfone","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rankin, D.C. (2015). Sedatives and tranquilizers. Vet. Anesth. Analg. Fifth Ed. Lumb Jones, 196\u2013206.","DOI":"10.1002\/9781119421375.ch10"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1467-2995.2005.00243.x","article-title":"A2-agonists in sheep: A review","volume":"33","year":"2006","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"154","DOI":"10.2460\/ajvr.1999.60.02.154","article-title":"Histopathologic alterations induced in the lungs of sheep by use of \u03b12-adrenergic receptor agonists","volume":"60","author":"Celly","year":"1999","journal-title":"Am. J. Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"267","DOI":"10.2460\/ajvr.79.3.267","article-title":"Effects of dexmedetomidine combined with commonly administered opioids on clinical variables in dogs","volume":"79","author":"Nishimura","year":"2018","journal-title":"Am. J. Vet. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.5326\/JAAHA-MS-7055","article-title":"2020 AAHA anesthesia and monitoring guidelines for dogs and cats","volume":"56","author":"Grubb","year":"2020","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"i1","DOI":"10.1136\/thoraxjnl-2015-206865","article-title":"British Thoracic Society guidelines for home oxygen use in adults: Accredited by NICE","volume":"70","author":"Hardinge","year":"2015","journal-title":"Thorax"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/vaa.12259","article-title":"Effects of intramuscular dexmedetomidine in combination with ketamine or alfaxalone in swine","volume":"43","author":"Santos","year":"2016","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"741","DOI":"10.2193\/0091-7648(2006)34[741:EOTPFI]2.0.CO;2","article-title":"Evaluation of the potential for injury with remote drug-delivery systems","volume":"34","author":"Cattet","year":"2006","journal-title":"Wildl. Soc. Bull."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1292\/jvms.15-0065","article-title":"Cardiorespiratory and anesthetic effects produced by the combination of butorphanol, medetomidine and alfaxalone administered intramuscularly in Beagle dogs","volume":"77","author":"Lee","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1292\/jvms.14-0200","article-title":"Sedative effects of intramuscular alfaxalone administered to cats","volume":"77","author":"Tamura","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1292\/jvms.14-0368","article-title":"The pharmacological effects of the anesthetic alfaxalone after intramuscular administration to dogs","volume":"77","author":"Tamura","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1638\/2013-0291.1","article-title":"Comparison of xylazine\u2013ketamine and dexmedetomidine\u2013ketamine anesthesia in captive Formosan serows (Capriconis Swinhoei)","volume":"45","author":"Yu","year":"2014","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chang, L.-J., Weng, H.-Y., Lien, C.-Y., and Chen, K.-S. (2024). Retrospective Comparison of the Anesthetic Effects of Tiletamine\u2013Zolazepam with Dexmedetomidine and Ketamine with Dexmedetomidine in Captive Formosan Serow (Capricornis swinhoei). Animals, 14.","DOI":"10.3390\/ani14101413"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1467-2995.2008.00428.x","article-title":"The cardiorespiratory and anesthetic effects of clinical and supraclinical doses of alfaxalone in cats","volume":"36","author":"Muir","year":"2009","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1467-2995.2008.00406.x","article-title":"Cardiorespiratory and anesthetic effects of clinical and supraclinical doses of alfaxalone in dogs","volume":"35","author":"Wiese","year":"2008","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/vaa.12006","article-title":"A comparison of cardiopulmonary and anesthetic effects of an induction dose of alfaxalone or propofol in dogs","volume":"40","author":"Maney","year":"2013","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2460\/ajvr.1994.55.03.363","article-title":"Hemodynamic and analgesic effects of propofol infusion in medetomidine-premedicated dogs","volume":"55","author":"Thurmon","year":"1994","journal-title":"Am. J. Vet. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1111\/jvp.12960","article-title":"Application of \u03b12-adrenergic agonists combined with anesthetics and their implication in pulmonary intravascular macrophages-insulted pulmonary edema and hypoxemia in ruminants","volume":"44","author":"Abouelfetouh","year":"2021","journal-title":"J. Vet. Pharmacol. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1136\/vr.129.14.310","article-title":"Effects of anticholinergic treatment on the cardiac and respiratory systems in dogs sedated with medetomidine","volume":"129","author":"Short","year":"1991","journal-title":"Vet. Rec."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/vaa.12166","article-title":"Chronotropic effect of propofol or alfaxalone following fentanyl administration in healthy dogs","volume":"42","author":"Okushima","year":"2015","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/j.vaa.2016.11.013","article-title":"Sedative and physiologic effects of low-dose intramuscular alfaxalone in dogs","volume":"44","author":"Maney","year":"2017","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0014-2999(88)90260-9","article-title":"Behavioural and neurochemical effects of medetomidine, a novel veterinary sedative","volume":"158","author":"MacDonald","year":"1988","journal-title":"Eur. J. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1136\/vr.160.26.891","article-title":"Evaluation of the clinical efficacy and safety of intramuscular and intravenous doses of dexmedetomidine and medetomidine in dogs and their reversal with atipamezole","volume":"160","author":"Granholm","year":"2007","journal-title":"Vet. Rec."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1002\/jwmg.383","article-title":"Immobilization of white-tailed deer with telazol, ketamine, and xylazine, and evaluation of antagonists","volume":"76","author":"Monteith","year":"2012","journal-title":"J. Wildl. Manag."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Caulkett, N., and Walzer, C. (2014). Wild sheep and goats. Zoo Anim. Wildl. Immobil. Anesth., 857\u2013862.","DOI":"10.1002\/9781118792919.ch62"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.vaa.2021.03.012","article-title":"The utility of a novel formulation of alfaxalone in a remote delivery system","volume":"48","author":"Patterson","year":"2021","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/jat.727","article-title":"A good practice guide to the administration of substances and removal of blood, including routes and volumes","volume":"21","author":"Diehl","year":"2001","journal-title":"J. Appl. Toxicol. Int. J."},{"key":"ref_38","first-page":"159","article-title":"Hyaluronidase, with xylazine and ketamine, reducing immobilization time in wild cattle (Bos taurus)","volume":"25","author":"Spadola","year":"2019","journal-title":"Large Anim. Rev."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/307\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:33:32Z","timestamp":1759919612000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,22]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ani15030307"],"URL":"https:\/\/doi.org\/10.3390\/ani15030307","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,22]]}}}