{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:21:14Z","timestamp":1790907674737,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Cattle and Sheep Industry Development Research Institute of Linxia","award":["KJJC-LX-2023-06"],"award-info":[{"award-number":["KJJC-LX-2023-06"]}]},{"name":"the Cattle and Sheep Industry Development Research Institute of Linxia","award":["24CXNA032"],"award-info":[{"award-number":["24CXNA032"]}]},{"name":"the Tianchi Talent Introduction Program of Xinjiang Uygur Autonomous Regions","award":["KJJC-LX-2023-06"],"award-info":[{"award-number":["KJJC-LX-2023-06"]}]},{"name":"the Tianchi Talent Introduction Program of Xinjiang Uygur Autonomous Regions","award":["24CXNA032"],"award-info":[{"award-number":["24CXNA032"]}]},{"name":"the Science and Technology Program of Gansu Province","award":["KJJC-LX-2023-06"],"award-info":[{"award-number":["KJJC-LX-2023-06"]}]},{"name":"the Science and Technology Program of Gansu Province","award":["24CXNA032"],"award-info":[{"award-number":["24CXNA032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>As the key components of dietary carbohydrates, ensuring a balance between forage-neutral detergent fiber (FNDF) and rumen-degradable starch (RDS) is essential for ruminant health. Eight male Hu sheep equipped with rumen cannulas were randomly divided into four groups based on dietary FNDF content: low FNDF (L-FNDF, 6.08%), middle low FNDF (ML-FNDF, 9.47%), middle high FNDF (MH-FNDF, 12.48%), and high FNDF (H-FNDF, 15.68%), while the RDS levels (15.65% of DM on average) were similar among the four groups. A replicated 4 \u00d7 4 Latin square design was employed in this study. The results indicated that mean and minimum ruminal pH increased linearly with increasing dietary FNDF content, while the duration and area of pH under 5.8 and 5.6, along with the acidosis index, reduced linearly (p \u2264 0.002). There were no differences between the MH-FNDF group and the H-FNDF group in these indicators (p &gt; 0.05). The molar proportions of acetate, butyrate, isobutyrate, and isovalerate, as well as the acetate-to-propionate ratio, increased linearly, while propionate and valerate molar proportions and lactate concentration displayed a linear decrease with increasing FNDF content in the diet (p &lt; 0.001). Increasing dietary FNDF content extended ruminating and chewing time while enhancing ruminal microbial diversity, promoting the proliferation of Fibrobacterota and Butyrivibrio in the rumen, and improving fiber degradability (p &lt; 0.05). When the dietary FNDF content exceeded 12.48%, no effects of FNDF on acetate to propionate ratio and fiber utilization were observed (p &gt; 0.05). The results suggest that augmenting FNDF content in the PTMR can reshape ruminal fermentation towards acetate production and promote rumination to enhance ruminal pH, thereby alleviating the risk of ruminal acidosis. When the RDS content in the PTMR was 15.57%, an FNDF content of 12.48% was optimal for maintaining stable ruminal function in sheep, and the recommended ratio of FNDF to RDS was 0.8.<\/jats:p>","DOI":"10.3390\/ani15030314","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T04:54:31Z","timestamp":1737608071000},"page":"314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Influence of Dietary Forage Neutral Detergent Fiber on Ruminal Fermentation, Chewing Activity, Nutrient Digestion, and Ruminal Microbiota of Hu Sheep"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhian","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongli","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-5643","authenticated-orcid":false,"given":"Xiuxiu","family":"Weng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuchun","family":"Sun","sequence":"additional","affiliation":[{"name":"Linxia Animal Husbandry Technical Extension Station, Linxia 731199, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhu","family":"He","sequence":"additional","affiliation":[{"name":"Linxia County Animal Husbandry Development Centre, Linxia 731804, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyu","family":"Meng","sequence":"additional","affiliation":[{"name":"Dongxiang County Animal Husbandry Development Centre, Linxia 731199, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoqing","family":"Liang","sequence":"additional","affiliation":[{"name":"Linxia State Xiaokangcun Feed Co., Linxia 731801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Li","sequence":"additional","affiliation":[{"name":"Hezheng County Animal Husbandry Development Centre, Linxia 731299, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5631","DOI":"10.3168\/jds.2020-19012","article-title":"Physically effective neutral detergent fiber improves chewing activity, rumen fermentation, plasma metabolites, and milk production in lactating dairy cows fed a high-concentrate diet","volume":"104","author":"Cao","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dong, H., Wang, S., Jia, Y., Ni, Y., Zhang, Y., Zhuang, S., Shen, X., and Zhao, R. (2013). Long-term effects of subacute ruminal acidosis (SARA) on milk quality and hepatic gene expression in lactating goats fed a high-concentrate diet. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082850"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8485","DOI":"10.3168\/jds.2022-21812","article-title":"Influence of dietary carbohydrate profile on the dairy cow rumen meta-proteome","volume":"105","author":"Mulakala","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.3168\/jds.2013-6895","article-title":"Effect of dietary physically effective fiber on ruminal fermentation and the fatty acid profile of milk in dairy goats","volume":"97","author":"Li","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"skae100","DOI":"10.1093\/jas\/skae100","article-title":"Effects of dietary forage neutral detergent fiber and rumen degradable starch ratios on chewing activity, ruminal fermentation, ruminal microbes and nutrient digestibility of Hu sheep fed a pelleted total mixed ration","volume":"102","author":"Zhang","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1017\/S1751731115002888","article-title":"Relative contribution of ruminal buffering systems to pH regulation in feedlot cattle fed either low- or high-forage diets","volume":"10","author":"Chibisa","year":"2016","journal-title":"Animal"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"skab011","DOI":"10.1093\/jas\/skab011","article-title":"Processing index of barley grain and dietary undigested neutral detergent fiber concentration affected chewing behavior, ruminal pH, and total tract nutrient digestibility of heifers fed a high-grain diet","volume":"99","author":"Ran","year":"2021","journal-title":"J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1017\/S1751731119001988","article-title":"Effects of finishing diet sorting and digestibility on performance and feed efficiency in beef steers","volume":"14","author":"Dykier","year":"2020","journal-title":"Animal"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100278","DOI":"10.1016\/j.animal.2021.100278","article-title":"Transcriptome analysis reveals the effect of high-grain pelleted and non-pelleted diets on ruminal epithelium of Hu-lamb","volume":"15","author":"Xue","year":"2021","journal-title":"Animal"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ma, X., Zhou, W., Guo, T., Li, F., Li, F., Ran, T., Zhang, Z., and Guo, L. (2021). Effects of dietary barley starch contents on the performance, nutrient digestion, rumen fermentation, and bacterial community of fattening Hu sheep. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.797801"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.3945\/jn.109.108506","article-title":"Epithelial capacity for apical uptake of short chain fatty acids is a key determinant for intraruminal pH and the susceptibility to subacute ruminal acidosis in sheep","volume":"139","author":"Penner","year":"2009","journal-title":"J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.3168\/jds.S0022-0302(91)78446-4","article-title":"Effect of fiber content and particle size of alfalfa silage on performance and chewing behavior","volume":"74","author":"Colenbrander","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"630","DOI":"10.3168\/jds.S0022-0302(03)73641-8","article-title":"Effects of particle size of alfalfa-based dairy cow diets on chewing activity, ruminal fermentation, and milk production","volume":"86","author":"Beauchemin","year":"2003","journal-title":"J. Dairy Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.3168\/jds.2013-7676","article-title":"Subacute ruminal acidosis challenge changed in situ degradability of feedstuffs in dairy goats","volume":"97","author":"Li","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_15","unstructured":"AOAC (2006). Official Methods of Analysis, Association of Official Analytical Chemists International. [18th ed.]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.3168\/jds.S0022-0302(97)76074-0","article-title":"Relationship between fermentation acid production in the rumen and the requirement for physically effective fiber","volume":"80","author":"Allen","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ma, Z., Zhang, X., Wang, R., Wang, M., Liu, T., and Tan, Z. (2020). Effects of Chemical and mechanical lysis on microbial dna yield, integrity, and downstream amplicon sequencing of rumen Bacteria and Protozoa. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.581227"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100902","DOI":"10.1016\/j.animal.2023.100902","article-title":"Variability in chewing, ruminal fermentation, digestibility and bacterial communities between subacute ruminal acidosis-susceptible and acidosis-tolerant sheep","volume":"17","author":"Zhang","year":"2023","journal-title":"Animal"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"501","DOI":"10.2527\/jas.2010-3013","article-title":"Effects of physically effective fiber on chewing activity, ruminal fermentation, and digestibility in goats","volume":"89","author":"Zhao","year":"2011","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"533","DOI":"10.3168\/jds.2012-5932","article-title":"Effect of cereal grain type and corn grain harvesting and processing methods on intake, digestion, and milk production by dairy cows through a meta-analysis","volume":"96","author":"Ferraretto","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4023","DOI":"10.2527\/jas.2015-9051","article-title":"Effect of flint corn processing method and roughage level on finishing performance of Nellore-based cattle","volume":"93","author":"Caetano","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.3168\/jds.S0022-0302(05)72807-1","article-title":"Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response","volume":"88","author":"Gozho","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7115","DOI":"10.1128\/AEM.00739-09","article-title":"Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis","volume":"75","author":"Khafipour","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.3168\/jds.S0022-0302(05)72888-5","article-title":"Effects of physically effective fiber on intake, chewing activity, and ruminal acidosis for dairy cows fed diets based on corn silage","volume":"88","author":"Beauchemin","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"E218","DOI":"10.2527\/jas2001.79E-SupplE218x","article-title":"Effects of grain variability and processing on starch utilization by lactating dairy cattle","volume":"79","author":"Firkins","year":"2001","journal-title":"J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10744","DOI":"10.3168\/jds.2021-20225","article-title":"Rumen microbiota and its relation to fermentation in lactose-fed calves","volume":"104","author":"Koike","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0749-0720(15)30102-X","article-title":"Ruminant adaptation to negative energy balance. Influences on the etiology of ketosis and fatty liver","volume":"16","author":"Herdt","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"666","DOI":"10.3168\/jds.2009-2349","article-title":"The effects of forage proportion and rapidly degradable dry matter from concentrate on ruminal digestion in dairy cows fed corn silage-based diets with fixed neutral detergent fiber and starch contents","volume":"93","author":"Lechartier","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Weimer, P.J. (2022). Degradation of cellulose and hemicellulose by ruminal microorganisms. Microorganisms, 10.","DOI":"10.3390\/microorganisms10122345"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4340","DOI":"10.3168\/jds.2019-17606","article-title":"Feeding diets varying in forage proportion and particle length to lactating dairy cows: I. Effects on ruminal pH and fermentation, microbial protein synthesis, digestibility, and milk production","volume":"103","author":"Li","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, J., Shi, H., Wang, Y., Li, S., Cao, Z., Ji, S., He, Y., and Zhang, H. (2017). Effect of dietary forage to concentrate ratios on dynamic profile changes and interactions of ruminal microbiota and metabolites in Holstein heifers. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02206"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"skad292","DOI":"10.1093\/jas\/skad292","article-title":"Rumen and cecum bacteria of beef cattle that differ in feed efficiency fed a forage diet","volume":"101","author":"Freetly","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1128\/AEM.06394-11","article-title":"Unexpected stability of bacteroidetes and firmicutes communities in laboratory biogas reactors fed with different defined substrates","volume":"78","author":"Kampmann","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s12223-012-0140-7","article-title":"Variability of Actinobacteria, a minor component of rumen microflora","volume":"57","author":"Sulak","year":"2012","journal-title":"Folia. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4697","DOI":"10.1128\/AEM.00720-15","article-title":"Ruminal bacterial community composition in dairy cows is dynamic over the course of two lactations and correlates with feed efficiency","volume":"81","author":"Jewell","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Li, F., Wang, Z., Dong, C., Li, F., Wang, W., Yuan, Z., Mo, F., and Weng, X. (2017). Rumen bacteria communities and performances of fattening lambs with a lower or greater subacute ruminal acidosis risk. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02506"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7482","DOI":"10.1128\/AEM.00388-10","article-title":"Rumen microbial population dynamics during adaptation to a high-grain diet","volume":"76","author":"Fernando","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Betancur-Murillo, C.L., Aguilar-Marin, S.B., and Jovel, J. (2022). Prevotella: A key player in ruminal metabolism. Microorganisms, 11.","DOI":"10.3390\/microorganisms11010001"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1126\/science.128.3322.474","article-title":"Volatile fatty acid growth factor for cellulolytic cocci of bovine rumen","volume":"128","author":"Allison","year":"1958","journal-title":"Science"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Han, L., Xue, W., Cao, H., Chen, X., Qi, F., Ma, T., Tu, Y., Diao, Q., Zhang, C., and Cui, K. (2021). Comparison of rumen fermentation parameters and microbiota of yaks from different altitude regions in Tibet, China. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.807512"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/314\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:34:20Z","timestamp":1759919660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,23]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ani15030314"],"URL":"https:\/\/doi.org\/10.3390\/ani15030314","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,23]]}}}