{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:04:58Z","timestamp":1791828298753,"version":"4.3.4"},"reference-count":48,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T00:00:00Z","timestamp":1737676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Introducing Urgently Needed Talents in Key Supporting Regions of Shandong Province","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Project of Introducing Urgently Needed Talents in Key Supporting Regions of Shandong Province","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Project of Introducing Urgently Needed Talents in Key Supporting Regions of Shandong Province","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]},{"name":"Earmarked Fund for CARS","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Earmarked Fund for CARS","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Earmarked Fund for CARS","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["2023TZXD044"],"award-info":[{"award-number":["2023TZXD044"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["CARS-43-B-1"],"award-info":[{"award-number":["CARS-43-B-1"]}]},{"name":"Special Economic Animal Industry Technology System of Shandong Province","award":["SDAIT-21-16"],"award-info":[{"award-number":["SDAIT-21-16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Three experiments were conducted to investigate the effects of grape seed proanthocyanidin extract (GSPE) on the growth performance of weaned Hyla rabbits and explore its protective effects against oxidative stress in the liver and intestine by establishing a hydrogen peroxide (H2O2)-induced oxidative stress model. In Exp.1, ninety-six weaned rabbits were used to evaluate the effects of dietary GSPE level on growth performance, and the results showed that a 400 mg\/kg GSPE addition increased the feed conversion ratio and liver coefficient and promoted cholesterol metabolism. Exp.2 was conducted to explore the H2O2 concentration required to establish an oxidative stress model, indicating that the model could be established by an intraperitoneal injection of 10% H2O2 at a dosage of 1 mL\/kg body weight. In Exp.3, seventy-two weaned rabbits were used to investigate the protective effects against H2O2-induced oxidative stress in the liver and intestine. Our findings showed that 400 mg\/kg GSPE supplementation could alleviate the adverse effects of H2O2 injection on the antioxidant capacity in the liver and intestine as well as liver morphology. Therefore, an addition of 400 mg\/kg GSPE could improve growth performance and alleviate H2O2-induced adverse effects on the liver and small intestine by enhancing the antioxidative capacity in weaned Hyla rabbits.<\/jats:p>","DOI":"10.3390\/ani15030327","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T03:26:31Z","timestamp":1737689191000},"page":"327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Grape Seed Proanthocyanidin Extract Improves Growth Performance and Protects Against Hydrogen Peroxide-Induced Oxidative Stress to the Liver and Intestine in Weaned Hyla Rabbits"],"prefix":"10.3390","volume":"15","author":[{"given":"Maohua","family":"Gong","sequence":"first","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science, Shandong Agricultural University, Tai\u2019an 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9262-2103","authenticated-orcid":false,"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science, Shandong Agricultural University, Tai\u2019an 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5670-8038","authenticated-orcid":false,"given":"Fuchang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science, Shandong Agricultural University, Tai\u2019an 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science, Shandong Agricultural University, Tai\u2019an 271017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Averill-Bates, D. (2024). Reactive oxygen species and cell signaling. Review. Biochim. Biophys. Acta (BBA)-Mol. Cell Res., 1871.","DOI":"10.1016\/j.bbamcr.2023.119573"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Machado, I.F., Miranda, R.G., Dorta, D.J., Rolo, A.P., and Palmeira, C.M. (2023). Targeting oxidative stress with polyphenols to fight liver diseases. Antioxidants, 12.","DOI":"10.3390\/antiox12061212"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41419-024-06939-5","article-title":"Oxidative cell death in cancer: Mechanisms and therapeutic opportunities","volume":"15","author":"An","year":"2024","journal-title":"Cell Death Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1089\/ars.2012.4729","article-title":"Mitochondria as a source of reactive oxygen and nitrogen species: From molecular mechanisms to human health","volume":"18","author":"Figueira","year":"2013","journal-title":"Antioxid. Redox Signal."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chen, J., Luo, Y., Li, Y., Chen, D., Yu, B., and He, J. (2021). Chlorogenic acid attenuates oxidative stress-induced intestinal epithelium injury by co-regulating the PI3K\/Akt and I\u03baB\u03b1\/NF-\u03baB signaling. Antioxidants, 10.","DOI":"10.3390\/antiox10121915"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1111\/j.1872-034X.2012.00996.x","article-title":"Oxidative stress and liver disease","volume":"42","author":"Zhu","year":"2012","journal-title":"Hepatol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"166","DOI":"10.5455\/javar.2018.e262","article-title":"Weaning induces changes in behavior and stress indicators in young New Zealand rabbits","volume":"5","author":"Gharib","year":"2018","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/jpn.13966","article-title":"Protective effect of chlorogenic acid on liver injury in heat-stressed meat rabbits","volume":"108","author":"Ji","year":"2024","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s12664-011-0090-z","article-title":"Protective role of lipoic acid on methotrexate induced intestinal damage in rabbit model","volume":"30","author":"Somi","year":"2011","journal-title":"Indian J. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rauf, A., Imran, M., Abu-Izneid, T., Patel, S., Pan, X., Naz, S., Silva, A.S., Saeed, F., and Suleria, H.A.R. (2019). Proanthocyanidins: A comprehensive review. Biomed. Pharmacother., 116.","DOI":"10.1016\/j.biopha.2019.108999"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/1745039X.2016.1139609","article-title":"Effects of grape seed extract as a natural antioxidant on growth performance, carcass characteristics and antioxidant status of rabbits during heat stress","volume":"70","author":"Hassan","year":"2016","journal-title":"Arch. Anim. Nutr."},{"key":"ref_12","first-page":"2139","article-title":"Impact of Grape Seed Extract (Proanthocyanidins) on Pathophysiological Changes and Antioxidant Capacity in Rabbit Does Expose to Heat Stress","volume":"55","author":"Ghodaia","year":"2024","journal-title":"Egypt. J. Vet. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rajput, S.A., Sun, L., Zhang, N., Khalil, M.M., Gao, X., Ling, Z., Zhu, L., Khan, F.A., Zhang, J., and Qi, D. (2017). Ameliorative effects of grape seed proanthocyanidin extract on growth performance, immune function, antioxidant capacity, biochemical constituents, liver histopathology and aflatoxin residues in broilers exposed to aflatoxin B1. Toxins, 9.","DOI":"10.3390\/toxins9110371"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"105066","DOI":"10.1016\/j.livsci.2022.105066","article-title":"Grape seed procyanidins improve intestinal health by modulating gut microbiota and enhancing intestinal antioxidant capacity in weaned piglets","volume":"264","author":"Wei","year":"2022","journal-title":"Livest. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Li, Y., Yu, B., Huang, Z., Luo, Y., Zheng, P., Mao, X., Yu, J., Tan, H., and Luo, J. (2024). Grape seed proanthocyanidins improves growth performance, antioxidative capacity, and intestinal microbiota in growing pigs. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1501211"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"170393","DOI":"10.1016\/j.scitotenv.2024.170393","article-title":"Transcriptomic analysis of hydrogen peroxide-induced liver dysfunction in Cyprinus carpio: Insights into protein synthesis and metabolism","volume":"917","author":"Feng","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"15479","DOI":"10.1039\/C4RA13557A","article-title":"Hydrogen peroxide-induced oxidative stress activates NF-\u03baB and Nrf2\/Keap1 signals and triggers autophagy in piglets","volume":"5","author":"Yin","year":"2015","journal-title":"Rsc Adv."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1080\/1828051X.2022.2109521","article-title":"Effects of enzymolytic soybean meal on the growth performance, digestive enzyme activity, some serum indexes, carcase performance and meat quality of Rex rabbits","volume":"21","author":"Kong","year":"2022","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e00654-21","DOI":"10.1128\/Spectrum.00654-21","article-title":"Supplementation with exogenous catalase from Penicillium notatum in the diet ameliorates lipopolysaccharide-induced intestinal oxidative damage through affecting intestinal antioxidant capacity and microbiota in weaned pigs","volume":"9","author":"Chen","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, P., Jing, C., Liang, M., Jiang, S., Huang, L., Jiao, N., Li, Y., and Yang, W. (2021). Zearalenone exposure triggered cecal physical barrier injury through the TGF-\u03b21\/Smads signaling pathway in weaned piglets. Toxins, 13.","DOI":"10.3390\/toxins13120902"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, Y., Zhao, X., Zhang, L., Zhan, X., Liu, Z., Zhuo, Y., Lin, Y., Fang, Z., Che, L., and Feng, B. (2020). Effects of a diet supplemented with exogenous catalase from penicillium notatum on intestinal development and microbiota in weaned piglets. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030391"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1017\/S1751731116002056","article-title":"Effects of dietary grape proanthocyanidins on the growth performance, jejunum morphology and plasma biochemical indices of broiler chicks","volume":"11","author":"Yang","year":"2017","journal-title":"Animal"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"215","DOI":"10.2478\/aoas-2022-0059","article-title":"Dietary Effect of Grape Seed Proanthocyanidin Extract on Growth Performance, Serum Biochemical Parameters, Skin Mucosal Immune Response, and Antioxidant Capacity in Goldfish (Carassius auratus)","volume":"23","author":"Jahanbakhshi","year":"2023","journal-title":"Ann. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"736689","DOI":"10.1016\/j.aquaculture.2021.736689","article-title":"Effects of diets containing grape seed proanthocyanidin extract on the growth and oxidative capacity of common carp (Cyprinus carpio)","volume":"540","author":"Mohammadi","year":"2021","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Li, Y., Yu, B., Luo, Y., Huang, Z., Zheng, P., Mao, X., Dai, Z., Yu, J., and Yan, H. (Anim. Nutr., 2024). Dietary supplementation of grape seed proanthocyanidins improves growth performance, carcass traits, and meat quality in growing-finishing pigs, Anim. Nutr., in press.","DOI":"10.2139\/ssrn.4907780"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kim, J., Yun, K., Cho, A., Kim, D.H., Lee, Y.K., Choi, M.J., Kim, S.-h., Kim, H., Yoon, J.W., and Park, H.C. (2022). High cortisol levels are associated with oxidative stress and mortality in maintenance hemodialysis patients. BMC Nephrol., 23.","DOI":"10.1186\/s12882-022-02722-w"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.tvjl.2006.06.005","article-title":"Oxidants and antioxidants in disease: Oxidative stress in farm animals","volume":"173","author":"Lykkesfeldt","year":"2007","journal-title":"Vet. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.5713\/ajas.2007.1600","article-title":"Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs","volume":"20","author":"Yuan","year":"2007","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4850","DOI":"10.2174\/092986712803341520","article-title":"Role of oxidative stress and molecular changes in liver fibrosis: A review","volume":"19","author":"Uribe","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"101816","DOI":"10.1016\/j.beem.2023.101816","article-title":"Nonalcoholic fatty liver disease and adult growth hormone deficiency: An under-recognized association?","volume":"37","author":"Takahashi","year":"2023","journal-title":"Best Pract. Res. Clin. Endocrinol. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.fct.2009.02.007","article-title":"Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats","volume":"47","author":"Yousef","year":"2009","journal-title":"Food Chem. Toxicol."},{"key":"ref_32","unstructured":"Mansouri, E., Panahi, M., Ghaffari, M.A., and Ghorbani, A. (2011). Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney. Iran. Biomed. J., 15."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9390810","DOI":"10.1155\/2018\/9390810","article-title":"Grape seed proanthocyanidin extract prevents ovarian aging by inhibiting oxidative stress in the hens","volume":"2018","author":"Liu","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Long, M., Yang, S.-H., Han, J.-X., Li, P., Zhang, Y., Dong, S., Chen, X., Guo, J., Wang, J., and He, J.-B. (2016). The protective effect of grape-seed proanthocyanidin extract on oxidative damage induced by zearalenone in Kunming mice liver. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17060808"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"114518","DOI":"10.1016\/j.anifeedsci.2020.114518","article-title":"Effects of high-concentrate diet supplemented with grape seed proanthocyanidins on growth performance, liver function, meat quality, and antioxidant activity in finishing lambs","volume":"266","author":"Mu","year":"2020","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_36","first-page":"4133","article-title":"Effects of grape seed proanthocyanidins extract on fat deposition and lipid metabolism in rats","volume":"33","author":"Gu","year":"2021","journal-title":"Chin. J. Anim. Nutr."},{"key":"ref_37","first-page":"4021","article-title":"Dietary grape seed proanthocyanidin extract supplementation improves antioxidant capacity and lipid metabolism in finishing pigs","volume":"34","author":"Feng","year":"2023","journal-title":"Anim. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S1542-3565(04)00622-6","article-title":"High-density lipoprotein cholesterol as an indicator of liver function and prognosis in noncholestatic cirrhotics","volume":"3","author":"Habib","year":"2005","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1385\/CT:3:4:331","article-title":"Grape seed proanthocyanidins induce pro-oxidant toxicity in cardiomyocytes","volume":"3","author":"Shao","year":"2003","journal-title":"Cardiovasc. Toxicol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"skab089","DOI":"10.1093\/jas\/skab089","article-title":"Dietary taurine attenuates hydrogen peroxide-impaired growth performance and meat quality of broilers via modulating redox status and cell death signaling","volume":"99","author":"Xing","year":"2021","journal-title":"J. Anim. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.numecd.2005.05.003","article-title":"A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress","volume":"15","author":"Stewart","year":"2005","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1093\/ajcn\/70.4.557","article-title":"Molecular and cellular responses to oxidative stress and changes in oxidation-reduction imbalance in the intestine","volume":"70","author":"Aw","year":"1999","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Li, X., Hua, Y., Yang, C., Liu, S., Tan, L., Guo, J., and Li, Y. (2023). Polysaccharides extracted from mulberry fruits (Morus nigra L.): Antioxidant effect of ameliorating H2O2-induced liver injury in HepG2 cells. BMC Complement. Med. Ther., 23.","DOI":"10.1186\/s12906-023-03925-w"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/35041687","article-title":"Oxidants, oxidative stress and the biology of ageing","volume":"408","author":"Finkel","year":"2000","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.envpol.2018.02.035","article-title":"Dietary grape seed proanthocyanidin extract regulates metabolic disturbance in rat liver exposed to lead associated with PPAR\u03b1 signaling pathway","volume":"237","author":"Yang","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/s41580-020-0278-0","article-title":"Cell fate specification and differentiation in the adult mammalian intestine","volume":"22","author":"Beumer","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Grandhaye, J., Douard, V., Rodriguez-Mateos, A., Xu, Y., Cheok, A., Riva, A., Guabiraba, R., Zemb, O., Philippe, C., and Monnoye, M. (2020). Microbiota changes due to grape seed extract diet improved intestinal homeostasis and decreased fatness in parental broiler hens. Microorganisms, 8.","DOI":"10.3390\/microorganisms8081141"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"7817","DOI":"10.1039\/D0FO01418D","article-title":"Grape seed proanthocyanidin extract ameliorates dextran sulfate sodium-induced colitis through intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokines and gut microbiota modulation","volume":"11","author":"Sheng","year":"2020","journal-title":"Food Funct."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/327\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:35:02Z","timestamp":1759919702000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,24]]},"references-count":48,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ani15030327"],"URL":"https:\/\/doi.org\/10.3390\/ani15030327","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,24]]}}}