{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:27:20Z","timestamp":1792175240137,"version":"4.4.0"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Pathogens and challenging conditions in fish farming are common problems that cause mortality and reduce growth performance. Phytogenic feed additives can support the immunity of fish and thereby improve performance, production, and disease resistance. Two experiments with Nile Tilapia aimed to evaluate the effects of a matrix-encapsulated phytogenic additive (Digestarom\u00ae P.E.P. MGE) in different feed formulations, which vary in the marine meal inclusion level (5% and 12.8%), on growth performance and resistance against Streptococcus agalactiae. Fish were stocked in a recirculating aquaculture tank system per experiment and were fed with diets with or without the phytogenic supplementation for 8 weeks (six replicates per group). After an intraperitoneal injection with a sterilized saline solution and bath immersion with S. agalactiae (5 \u00d7 106 CFU\/mL), mortality of the fish was recorded for 20 days. Independent of the diet formulation, the supplementation with the phytogenic feed additive significantly improved the fish production; feed conversion ratio; immune response (mainly lactic acid dehydrogenase, differential blood cell counts, and the lysozyme activity in blood); and fish survival after the S. agalactiae challenge. Thus, phytogenic feed additives are promising strategies to improve Nile tilapia production by enhancing fish growth performance, health, and protection against S. agalactiae infections.<\/jats:p>","DOI":"10.3390\/ani15030380","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T03:26:10Z","timestamp":1738121170000},"page":"380","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Phytogenic Feed Additives as a Sustainable Alternative to Antibiotics: Enhancing Growth and Disease Resistance in Nile Tilapia (Oreochromis niloticus)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4919-1656","authenticated-orcid":false,"given":"Christina","family":"Gruber","sequence":"first","affiliation":[{"name":"Animal Nutrition & Health R&D Center, DSM-Firmenich, 3430 Tulln, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimira","family":"Ocelova","sequence":"additional","affiliation":[{"name":"Animal Nutrition & Health R&D Center, DSM-Firmenich, 3430 Tulln, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7222-3615","authenticated-orcid":false,"given":"Jutta C.","family":"Kesselring","sequence":"additional","affiliation":[{"name":"Animal Nutrition & Health R&D Center, DSM-Firmenich, 3430 Tulln, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Wein","sequence":"additional","affiliation":[{"name":"Animal Nutrition & Health R&D Center, DSM-Firmenich, 3430 Tulln, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"ref_1","unstructured":"FAO (2024, August 05). The State of World Fisheries and Aquaculture. Available online: https:\/\/openknowledge.fao.org\/server\/api\/core\/bitstreams\/c35c5020-28ca-4555-b857-e9b67a7f321e\/content\/cd0683en.html."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1111\/jwas.12674","article-title":"The key role of functional aquafeeds to achieve a more sustainable aquaculture","volume":"50","author":"Leal","year":"2019","journal-title":"J. World Aquac. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-018-0562-6","article-title":"Phytochemicals as antibiotic alternatives to promote growth and enhance host health","volume":"49","author":"Lillehoj","year":"2018","journal-title":"Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Firmino, J.P., Galindo-Villegas, J., Reyes-L\u00f3pez, F.E., and Gisbert, E. (2021). Phytogenic bioactive compounds shape fish mucosal immunity. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.695973"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.70102\/IJARES\/V2I2\/6","article-title":"A review on the application of phytogenics as feed additives for aquatic animals","volume":"2","author":"Kazempoor","year":"2022","journal-title":"Int. J. Aquat. Res. Environ. Stud."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.aninu.2024.01.012","article-title":"Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade","volume":"17","author":"Wang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1753-5131.2011.01048.x","article-title":"Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in finfish culture","volume":"3","author":"Chakraborty","year":"2011","journal-title":"Rev. Aquac."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.aquaculture.2014.05.048","article-title":"Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future perspectives","volume":"433","author":"Reverter","year":"2014","journal-title":"Aquaculture"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1111\/raq.12197","article-title":"Plant essential oils as fish diet additives: Benefits on fish health and stability in feed","volume":"10","author":"Sutili","year":"2018","journal-title":"Rev. Aquac."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/raq.12021","article-title":"Application of phytochemicals as growth-promoters and endocrine modulators in fish culture","volume":"6","author":"Chakraborty","year":"2014","journal-title":"Rev. Aquac."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.fsi.2015.01.027","article-title":"Effects of a phytogenic feed additive on growth performance, susceptibility of channel catfish to Edwardsiella ictaluri and levels of mannose binding lectin","volume":"44","author":"Peterson","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kesselring, J., Gruber, C., Standen, B., and Wein, S. (2019). Effect of a phytogenic feed additive on the growth performance and immunity of pacific white leg shrimp, Litopenaeus vannamei, fed a low fishmeal diet. Fish Shellfish Immunol., 91.","DOI":"10.1016\/j.fsi.2019.04.155"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1111\/anu.12854","article-title":"Citrus x aurantium essential oil as feed additive improved growth performance, survival, metabolic, and oxidative parameters of silver catfish (Rhamdia quelen)","volume":"25","author":"Lopes","year":"2019","journal-title":"Aquac. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2010.03.007","article-title":"Essential oils in poultry nutrition: Main effects and modes of action","volume":"158","author":"Brenes","year":"2010","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/ffj.1967","article-title":"Essential oils and aromatic plants in animal feeding\u2014A European perspective. A review","volume":"25","author":"Franz","year":"2010","journal-title":"Flavour Fragr. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"521","DOI":"10.17221\/8594-CJAS","article-title":"Current use of phytogenic feed additives in animal nutrition: A review","volume":"60","year":"2015","journal-title":"Czech J. Anim. Sci."},{"key":"ref_17","unstructured":"Nates, S.F. (2016). Functional feed additives in aquaculture feeds. Aquafeed Formulation, Elsevier Inc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/jwas.12495","article-title":"Effect of Dietary Essential Oils Supplementation on Growth Performance, Nutrient Utilization, and Protein Digestibility of Juvenile Gilthead Seabream Fed a Low-Fishmeal Diet","volume":"49","author":"Rodrigues","year":"2018","journal-title":"J. World Aquac. Soc."},{"key":"ref_19","unstructured":"El-Sayed, A.-F.M. (2020). Tilapia Culture, Academic Press. [2nd ed.]. Available online: https:\/\/books.google.at\/books?hl=de&lr=&id=yhy3DwAAQBAJ&oi=fnd&pg=PP1&dq=tilapia&ots=OIKwx9nTjj&sig=ePQc-dg0WD1sqgVoH-okwffIRzw&redir_esc=y#v=onepage&q=tilapia&f=false."},{"key":"ref_20","unstructured":"Klesius, P.H., Shoemaker, C., and Evans, J. (2008, January 12\u201314). Epidemiology of Streptococcus agalactiae and streptococcosis in tilapia (Oreochromis sp.). Proceedings from the 8th International Symposium on Tilapia in Aquaculture, Cairo, Egypt."},{"key":"ref_21","unstructured":"iConcept Press Ltd. (2014). Epidemiology of Streptococcus agalactiae and streptococcosis in tilapia (Oreochromis sp.). Epidemiology: Theory, Research and Practice, iConcept Press Ltd.. [1st ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.aquaculture.2011.09.011","article-title":"Evaluating a nested-PCR assay for detecting Streptococcus agalactiae in red tilapia (Oreochromis sp.) tissue","volume":"321","author":"Junca","year":"2011","journal-title":"Aquaculture"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1365-2761.2010.01220.x","article-title":"Streptococcus dysgalactiae as an agent of septicaemia in Nile tilapia, Oreochromis niloticus (L.)","volume":"34","author":"Netto","year":"2011","journal-title":"J. Fish Dis."},{"key":"ref_24","unstructured":"EU: Commission Regulation (EC) No 152\/2009 of 27 January 2009 (2009). Laying down the Methods of Sampling and Analysis for the Official Control of Feed. Off. J. Eur. Union., 54, 1\u2013130."},{"key":"ref_25","unstructured":"AOCS (2005). Official Method Ba 6a-05. Official Methods and Recommended Practices of the AOCS, The American Oil Chemists\u2019 Society."},{"key":"ref_26","unstructured":"(1999). Animal feeding stuffs\u2014Determination of fat content (Standard No. ISO 6492:1999)."},{"key":"ref_27","unstructured":"AOAC International (2016). AOAC Official Method 2001.11. Protein (crude) in animal feed, forage (plant tissue), grain, and oilseeds block digestion method using copper catalyst and steam distillation into boric acid. Official methods of analysis of AOAC International, AOAC International. [20th ed.]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1365-2109.2004.01201.x","article-title":"Effect of dietary protein levels and feeding rates on growth performance, production traits and body composition of Nile tilapia, Oreochromis niloticus (L.) cultured in concrete tanks","volume":"36","author":"Gaber","year":"2005","journal-title":"Aquac. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3181\/00379727-119-30188","article-title":"A rapid and sensitive assay of muramidase","volume":"119","author":"Chandan","year":"1965","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/gcen.1997.6938","article-title":"Effect of confinement stress on circulating levels of growth hormone and two prolactins in freshwater-adapted tilapia (Oreochromis niloticus)","volume":"108","author":"Auperin","year":"1997","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_31","first-page":"49","article-title":"Anesthetic dosages of quinaldine sulfate and 2-phenoxyethanol for induction, maintenance and lethal dose 50 in the juvenile hybrid tilapia","volume":"9","author":"Tengjaroenkul","year":"2004","journal-title":"KKU Res. J."},{"key":"ref_32","unstructured":"Bui Chau Truc, D., Gruber, C., Kesselring, J., Standen, B., and Wein, S. (2024, January 5\u20137). Effect of a Phytogenic Feed Additive on the Growth Performance, Immune Response and Resistance against Streptococcus agalactiae of Nile Tilapia, Oreochromis niloticus. Proceedings of the 78th Conference of the Society of Nutrition Physiology, G\u00f6ttingen, Germany."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.aaf.2020.08.009","article-title":"Efficacy of phytogenic extracts on growth performance and health of tilapia (Oreochromis niloticus \u00d7 O. aureus)","volume":"7","author":"Poolsawat","year":"2022","journal-title":"Aquac. Fish."},{"key":"ref_34","first-page":"113","article-title":"The Effects of Pollution on Haematological Parameters of Black Goby (Gobius niger L., 1758) in Fo\u00e7a and Alia\u011fa Bays","volume":"1\u20132","author":"Katalay","year":"2004","journal-title":"J. Fish Aquat. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jobaz.2015.03.002","article-title":"Hematological and biochemical characters of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) cultivated using methyltestosterone","volume":"72","author":"Sayed","year":"2015","journal-title":"J. Basic Appl. Zool."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1939-165X.2000.tb00389.x","article-title":"Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid)","volume":"29","author":"Hrubec","year":"2000","journal-title":"Vet. Clin. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1111\/j.1365-2109.2004.01167.x","article-title":"Comparative study of biochemical parameters in response to stress in Oreochromis aureus, O. mossambicus and two strains of O. niloticus","volume":"35","author":"Cnaani","year":"2004","journal-title":"Aquac. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.sjbs.2010.04.009","article-title":"Effect of different stress factors on some physiological parameters of Nile tilapia (Oreochromis niloticus)","volume":"17","year":"2010","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_39","first-page":"191","article-title":"Effects of energetic diets on growth, blood chemistry and liver pathology of African catfish (Clarias gariepinus)","volume":"58","author":"Yilmaz","year":"2006","journal-title":"Isr. J. Aquac."},{"key":"ref_40","first-page":"284","article-title":"Herbs\/spices as feed additive in aquaculture","volume":"2","author":"Asimi","year":"2013","journal-title":"J. Pure Appl. Sci. Technol."},{"key":"ref_41","unstructured":"Gherescu, P., Mihailov, S., and Grozea, A. (2023). The Influence of Some Phyto-Additives on Bio-Productive Performances and the Health of the Farmed Fish\u2013Review. J. Anim. Sci. Biotechnol., 56."},{"key":"ref_42","first-page":"148","article-title":"Anemia in teleost fishes","volume":"35","author":"Witeska","year":"2015","journal-title":"Bull. Eur. Ass. Fish Pathol."},{"key":"ref_43","unstructured":"Ergena, A., Natarajan, P., and Bedewi, Z. (2023). Effect of Ginger (Zingiber officinale) on Hematological Parameters and Resistant to Aeromonas hydrophila Infection in Nile Tilapia, Oreochromis niloticus L.. Aquac. Res. Dev., 14."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.fsi.2012.09.003","article-title":"Interacting effects of water temperature and dietary protein level on hematological parameters in Nile tilapia juveniles, Oreochromis niloticus (L.) and mortality under Streptococcus iniae infection","volume":"34","author":"Qiang","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ragland, S.A., and Criss, A.K. (2017). From bacterial killing to immune modulation: Recent insights into the functions of lysozyme. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006512"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1189\/jlb.3HI0215-064R","article-title":"Neutrophil contributions to the induction and regulation of the acute inflammatory response in teleost fish","volume":"99","author":"Havixbeck","year":"2016","journal-title":"J. Leukoc. Biol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/380\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:37:59Z","timestamp":1759919879000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/3\/380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,28]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ani15030380"],"URL":"https:\/\/doi.org\/10.3390\/ani15030380","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,28]]}}}