{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:54:22Z","timestamp":1789606462393,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Komipharm and the National Institute of Wildlife Disease Control, Ministry of Environment","award":["NIWDC-2023-RP-002"],"award-info":[{"award-number":["NIWDC-2023-RP-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>African swine fever virus (ASFV) has substantially spread worldwide, resulting in significant economic losses in the swine industry. Despite extensive research, no ASF vaccine has surpassed the effectiveness of live attenuated vaccines. For instance, the live attenuated vaccine ASFV-G-\u0394I177L\/\u0394LVR has demonstrated good efficacy and safety, along with prolonged persistence of ASF antibodies after vaccination. Therefore, we aimed to evaluate its potential for protection against highly virulent homologous ASF viruses based on changes in the farm environment. To this end, we challenged domestic pigs with a virulent field strain of ASFV following intramuscular immunization with ASFV-G-\u0394I177L\/\u0394LVR. We further assessed its genomic stability and long-term antibody persistence in immunized domestic pigs. All vaccinated pigs exhibited high antibody positivity, with higher levels of antibodies observed at the time of challenge. These high ASF vaccine antibodies were maintained for approximately 2 months after vaccination. In addition, no organ or tissue damage was observed in the vaccinated animals. Our findings demonstrate the applicability of this vaccine candidate in the prevention of ASFV infection in the swine industry.<\/jats:p>","DOI":"10.3390\/ani15040473","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T09:02:42Z","timestamp":1738918962000},"page":"473","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["African Swine Fever Vaccine Candidate ASFV-G-\u0394I177L\/\u0394LVR Protects Against Homologous Virulent Challenge and Exhibits Long-Term Maintenance of Antibodies"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7770-5022","authenticated-orcid":false,"given":"Sun A","family":"Choi","sequence":"first","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4078-8310","authenticated-orcid":false,"given":"Yeonji","family":"Kim","sequence":"additional","affiliation":[{"name":"Wildlife Disease Response Team, National Institute of Wildlife Disease Control and Prevention (NIWDC), Ministry of Environment, Gwangju 62407, Republic of Korea"},{"name":"College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Cheol","family":"Moon","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keun Seung","family":"Ahn","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghua","family":"Zheng","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Soon","family":"Kim","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Pyo","family":"Shin","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7499-4253","authenticated-orcid":false,"given":"Dongseob","family":"Tark","sequence":"additional","affiliation":[{"name":"Laboratory for Infectious Disease Prevention, College of Veterinary Medicine, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonjun","family":"Kim","sequence":"additional","affiliation":[{"name":"Wildlife Disease Response Team, National Institute of Wildlife Disease Control and Prevention (NIWDC), Ministry of Environment, Gwangju 62407, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongwoo","family":"Shin","sequence":"additional","affiliation":[{"name":"Wildlife Disease Response Team, National Institute of Wildlife Disease Control and Prevention (NIWDC), Ministry of Environment, Gwangju 62407, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weonhwa","family":"Jheong","sequence":"additional","affiliation":[{"name":"Wildlife Disease Response Team, National Institute of Wildlife Disease Control and Prevention (NIWDC), Ministry of Environment, Gwangju 62407, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9734-5852","authenticated-orcid":false,"given":"Jung Hyang","family":"Sur","sequence":"additional","affiliation":[{"name":"Central Research and Development Institute, Komipharm International Co., Ltd., Siheung-si 15094, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cwynar, P., Stojkov, J., and Wlazlak, K. (2019). African swine fever status in Europe. Viruses, 11.","DOI":"10.3390\/v11040310"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"104861","DOI":"10.1016\/j.prevetmed.2019.104861","article-title":"African swine fever emerging in China: Distribution characteristics and high-risk areas","volume":"175","author":"Ma","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105602","DOI":"10.1016\/j.actatropica.2020.105602","article-title":"One year of African swine fever outbreak in China","volume":"211","author":"Tao","year":"2020","journal-title":"Acta Tropica."},{"key":"ref_4","unstructured":"World Organization for Animal Health (2024, January 29). African Swine Fever (ASF)\u2014Situation Report 46. Available online: https:\/\/www.woah.org\/app\/uploads\/2024\/02\/asf-report46.pdf."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, Z., Chen, W., Qiu, Z., Li, Y., Fan, J., Wu, K., Li, X., Zhao, M., Ding, H., and Fan, S. (2022). African Swine Fever Virus: A Review. Life, 12.","DOI":"10.3390\/life12081255"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.virusres.2012.10.020","article-title":"African swine fever virus replication and genomics","volume":"173","author":"Dixon","year":"2013","journal-title":"Virus Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Galindo, I., and Alonso, C. (2017). African swine fever virus: A review. Viruses, 9.","DOI":"10.3390\/v9050103"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/JCM.43.1.112-119.2005","article-title":"Preclinical diagnosis of African swine fever virus in contact-exposed swine by a real-time PCR assay","volume":"43","author":"Zsak","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"197676","DOI":"10.1016\/j.virusres.2019.197676","article-title":"African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation","volume":"271","author":"Gallardo","year":"2019","journal-title":"Virus Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Chen, S., Wang, T., Luo, R., Lu, Z., Lan, J., Fu, Q., and Qiu, H.-J. (2024). Genetic variations of African swine fever virus: Major challenges and prospects. Viruses, 16.","DOI":"10.3390\/v16060913"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.tvjl.2017.12.025","article-title":"African swine fever: A re-emerging viral disease threatening the global pig industry","volume":"233","author":"Montoya","year":"2018","journal-title":"Vet. J."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rogoll, L., G\u00fcttner, A.K., Schulz, K., Bergmann, H., Staubach, C., Conraths, F.J., and Sauter-Louis, C. (2023). Seasonal occurrence of African swine fever in wild boar and domestic pigs in EU member states. Viruses, 15.","DOI":"10.3390\/v15091955"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"730","DOI":"10.5713\/ab.23.0342","article-title":"Pig production in Africa: Current status, challenges, prospects and opportunities","volume":"37","author":"Adesehinwa","year":"2024","journal-title":"Anim. Biosci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Brake, D.A. (2022). African swine fever modified live vaccine candidates: Transitioning from discovery to product development through harmonized standards and guidelines. Viruses, 14.","DOI":"10.3390\/v14122619"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gladue, D.P., and Borca, M.V. (2022). Recombinant ASF live attenuated virus strains as experimental vaccine candidates. Viruses, 14.","DOI":"10.3390\/v14050878"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1111\/tbed.14191","article-title":"African swine fever vaccines: Turning a dream into reality","volume":"68","author":"Jurado","year":"2021","journal-title":"Transbound Emerg. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sang, H., Miller, G., Lokhandwala, S., Sangewar, N., Waghela, S.D., Bishop, R.P., and Mwangi, W. (2020). Progress toward development of effective and safe African swine fever virus vaccines. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00084"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rock, D.L. (2021). Thoughts on African swine fever vaccines. Viruses, 13.","DOI":"10.3390\/v13050943"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e02017-19","DOI":"10.1128\/JVI.02017-19","article-title":"Development of a highly effective African swine fever virus vaccine by deletion of the I177L gene results in sterile immunity against the current epidemic Eurasia strain","volume":"94","author":"Borca","year":"2020","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00123-21","DOI":"10.1128\/JVI.00123-21","article-title":"A cell culture-adapted vaccine virus against the current African swine fever virus pandemic strain","volume":"95","author":"Borca","year":"2021","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Teklue, T., Wang, T., Luo, Y., Hu, R., and Qin, H.-J. (2020). Generation and evaluation of an African swine fever virus mutant with deletion of the CD2v and UK genes. Vaccines, 8.","DOI":"10.3390\/vaccines8040763"},{"key":"ref_22","first-page":"e497","article-title":"African swine fever virus vaccine candidate ASFV-G-\u2206I177L efficiently protects European and native pig breeds against circulating Vietnamese field strain","volume":"69","author":"Tran","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Tran, X.H., Phuong, L.T.T., Huy, N.Q., Thuy, D.T., Nguyen, V.D., Quang, P.H., Ng\u00f4n, Q.V., Rai, A., Gay, C.G., and Gladue, D.P. (2022). Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-\u2206I177L. Viruses, 14.","DOI":"10.3390\/v14050896"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Xie, Z., Liu, Y., Di, D., Liu, J., Gong, L., Chen, Z., Li, Y., Yu, W., Lv, L., and Zhong, Q. (2022). Protection evaluation of a five-gene-deleted African swine fever virus vaccine candidate against homologous challenge. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.902932"},{"key":"ref_25","unstructured":"World Organization for Animal Health (2023, September 04). Report of the Meeting of the WOAH Biological Standards Commission. Available online: https:www.woah.org\/app\/uploads\/2023\/10\/a-bsc-report-sept-2023-6.pdf."},{"key":"ref_26","first-page":"493","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0368-1742(21)80031-4","article-title":"On a form of swine fever occurring in British East Africa (Kenya Colony)","volume":"34","author":"Montgomery","year":"1921","journal-title":"J. Comp. Pathol. Ther."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8556","DOI":"10.1128\/JVI.00969-15","article-title":"African swine fever virus Georgia 2007 with a deletion of virulence-associated gene 9GL (B119L), when administrated at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge","volume":"89","author":"Holinka","year":"2015","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e01760-16","DOI":"10.1128\/JVI.01760-16","article-title":"Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate offers increased safety and protection against homologous challenge","volume":"91","author":"Risatti","year":"2017","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e01058-17","DOI":"10.1128\/JVI.01058-17","article-title":"BA71\u0394CD2: A new recombinant live attenuated African swine fever virus with cross-protective capabilities","volume":"91","author":"Monteagudo","year":"2017","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s11427-020-1657-9","article-title":"A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs","volume":"63","author":"Chen","year":"2020","journal-title":"Sci. China Life Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6048","DOI":"10.1128\/JVI.00554-15","article-title":"African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with virulent parental virus","volume":"89","author":"Holinka","year":"2015","journal-title":"J. Virol."},{"key":"ref_33","unstructured":"United States Department of Agriculture (2024, January 29). Notice of Withdrawal of Select Agent Regulatory Exclusion for African Swine Fever Virus-G-\u0394I177L, Available online: https:\/\/www.govinfo.gov\/content\/pkg\/FR-2024-05-16\/pdf\/2024-10770.pdf."},{"key":"ref_34","unstructured":"United States Department of Agriculture (2024, January 29). Notice of Withdrawal of Select Agent Regulatory Exclusions for Two Strains of African Swine Fever Virus, Available online: https:\/\/www.govinfo.gov\/content\/pkg\/FR-2022-10-27\/pdf\/2022-23446.pdf."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1038\/s41467-023-38868-w","article-title":"Highly lethal genotype I and II recombinant African swine fever viruses detected in pigs","volume":"14","author":"Zhao","year":"2023","journal-title":"Nat. Commun."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/4\/473\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:28:42Z","timestamp":1760027322000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/4\/473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ani15040473"],"URL":"https:\/\/doi.org\/10.3390\/ani15040473","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]}}}