{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:40:05Z","timestamp":1787496005651,"version":"build-2736575974"},"reference-count":79,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CONAHCYT (Mexico) grants","award":["003797"],"award-info":[{"award-number":["003797"]}]},{"name":"CONAHCYT (Mexico) grants","award":["CD.CBS.481.2021."],"award-info":[{"award-number":["CD.CBS.481.2021."]}]},{"name":"CONAHCYT (Mexico) grants","award":["IN212724"],"award-info":[{"award-number":["IN212724"]}]},{"name":"scholarships 1167792","award":["003797"],"award-info":[{"award-number":["003797"]}]},{"name":"scholarships 1167792","award":["CD.CBS.481.2021."],"award-info":[{"award-number":["CD.CBS.481.2021."]}]},{"name":"scholarships 1167792","award":["IN212724"],"award-info":[{"award-number":["IN212724"]}]},{"name":"divisional financing","award":["003797"],"award-info":[{"award-number":["003797"]}]},{"name":"divisional financing","award":["CD.CBS.481.2021."],"award-info":[{"award-number":["CD.CBS.481.2021."]}]},{"name":"divisional financing","award":["IN212724"],"award-info":[{"award-number":["IN212724"]}]},{"name":"PAPIIT UNAM","award":["003797"],"award-info":[{"award-number":["003797"]}]},{"name":"PAPIIT UNAM","award":["CD.CBS.481.2021."],"award-info":[{"award-number":["CD.CBS.481.2021."]}]},{"name":"PAPIIT UNAM","award":["IN212724"],"award-info":[{"award-number":["IN212724"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Reptiles, as ectothermic organisms, rely on environmental temperatures for optimal physiological performance. The thermal requirements for optimal locomotion in reptiles can severely affect their reproduction. However, some species are successful in environments with temperatures exceeding 40 \u00b0C. Holbrookuia propinqua is a species that reproduces at high temperatures, a difference from most species in the family Phrynosomatidae, to which it belongs. Adult male H. propinqua specimens were collected at their reproductive stage, kept in separate terrariums, and divided into four groups that were exposed to different temperatures. After seven days of exposure, both the testes and epididymides were removed. The organs were processed for immunohistochemistry and to determine SOD, CAT, and GPX activity. A sperm sample was obtained from the vas deferens. The GSI decreased with temperature, and the EI presented its maximum value at 24 \u00b0C. Sperm viability was lower at the highest temperature, and motility at 28 and 32 \u00b0C exceeded 90%. The specific SOD activity in the testis at 41 \u00b0C decreased by ~75%. In the epididymis, SOD activity decreased at 28 and 32 \u00b0C. Transient Receptor Potential Vanilloid 1 (TRPV1) increased after 28 \u00b0C at the interstitial level and increased almost twofold in the seminiferous tubules.<\/jats:p>","DOI":"10.3390\/ani15050656","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T10:52:47Z","timestamp":1740394367000},"page":"656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical Mechanisms That Buffer the Effects of High Temperatures in the Sand-Dwelling Lizard Holbrookia propinqua"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0145-8127","authenticated-orcid":false,"given":"Yessica","family":"Caballero V\u00e1zquez","sequence":"first","affiliation":[{"name":"Maestr\u00eda en Biolog\u00eda de la Reproducci\u00f3n Animal, Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-7316","authenticated-orcid":false,"given":"Ahiezer","family":"Rodr\u00edguez-Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Morfofisiolog\u00eda y Bioqu\u00edmica del Espermatozoide, Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5084-1276","authenticated-orcid":false,"given":"Fausto Roberto","family":"M\u00e9ndez de la Cruz","sequence":"additional","affiliation":[{"name":"Laboratorio de Herpetolog\u00eda, Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2534-9351","authenticated-orcid":false,"given":"Edith","family":"Arenas-R\u00edos","sequence":"additional","affiliation":[{"name":"Laboratorio de Morfofisiolog\u00eda y Bioqu\u00edmica del Espermatozoide, Departamento de Biolog\u00eda de la Reproducci\u00f3n, Universidad Aut\u00f3noma Metropolitana, Iztapalapa 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cossins, A.R., and Bowler, K. 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