{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T16:46:39Z","timestamp":1764002799900,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Program of National Natural Science Foundation of China","award":["GRANT32090023","2023-JB-12"],"award-info":[{"award-number":["GRANT32090023","2023-JB-12"]}]},{"name":"Chinese Academy of Engineering","award":["GRANT32090023","2023-JB-12"],"award-info":[{"award-number":["GRANT32090023","2023-JB-12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Brandt\u2019s vole is a common small rodent, and its gut microbiota is critical to host health and immune function. The parasitic fleas commonly found in Brandt\u2019s voles cause an immune response, but their impact on the gut microbiota remains unclear. According to the level of flea infestation, Brandt\u2019s voles were divided into the control group, low-infestation group, and high-infestation group. The changes in the microbial community composition, abundance, and diversity of the gut microbiota were evaluated using 16S rRNA sequencing. Flea infestation significantly affected body weight, food intake, and gut microbiota structure. The low-infestation group exhibited the most pronounced changes in weight and food intake, while the high-infestation group showed the least. In the 4th week, 16S rRNA sequencing revealed an increase in alpha diversity and alterations in microbial composition. Beta-diversity analysis indicated significant differences in the intestinal microbiota between the experimental groups and the control group. By the 8th week, these differences had diminished, suggesting that the microbiota had stabilized or recovered over time. Overall, parasitic flea infestation significantly alters the diversity, structure, and characteristic microbial enrichment of the gut microbiota in Brandt\u2019s voles, potentially impacting host metabolism, immunity, and growth. While this study lasted 8 weeks, the long-term health effects of flea infestation may persist. Future research should elucidate the interaction mechanisms between parasites and hosts, define the time frames and mechanisms of these long-term impacts, and provide theoretical support for animal health management and disease control.<\/jats:p>","DOI":"10.3390\/ani15050669","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T12:01:17Z","timestamp":1740484877000},"page":"669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Different Levels of Flea Infestation on Gut Microbiota of Brandt\u2019s Voles (Lasiopodomys brandtii) in China"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhenxu","family":"Wang","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenran","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinchang","family":"Lun","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqiang","family":"Ji","sequence":"additional","affiliation":[{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Shang","sequence":"additional","affiliation":[{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4066-7988","authenticated-orcid":false,"given":"Qiyong","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"},{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref_1","first-page":"143","article-title":"Epidemic status of flea-borne natural epidemic diseases in China","volume":"31","author":"Yang","year":"2015","journal-title":"J. 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