{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:01:02Z","timestamp":1760058062683,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","award":["Fondecyt Iniciaci\u00f3n 11220188"],"award-info":[{"award-number":["Fondecyt Iniciaci\u00f3n 11220188"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Placental samples for RNA extraction are collected via non-recovery (euthanasia) or invasive (surgery) methods in small ruminants, such as sheep. Alternatively, delivered placentas could be used, but the feasibility of obtaining high-quality RNA from this tissue is unknown in sheep. We aimed to evaluate the possibility of extracting RNA from naturally delivered ovine placenta, comparing two preservation methods. Twenty-seven single-pregnant sheep were monitored 24\/7 from gestational day 140 to parturition. Tissue was collected after placental delivery, preserved using snap frozen (SF, n = 27) and RNAlater\u00ae (LTR, n = 27) techniques, and processed for RNA extraction using a commercial kit. RNA concentration (ng\/\u00b5L), A260\/280, and RNA quality number (RQN) were measured. Concentration was higher (p &lt; 0.001) in LTR (70.39 \u00b1 6.3) than in SF (49.77 \u00b1 10.5), A260\/280 was higher (p = 0.045) in SF (2.06 \u00b1 0.01) than in LTR (2.03 \u00b1 0.01), and RQN was higher (p &lt; 0.0001) in SF (6.81 \u00b1 0.24) than in LTR (2.84 \u00b1 0.24) samples. Timing of placental delivery did not affect the evaluated indicators. Results indicate that extracting high-quality RNA from delivered placentas preserved via the snap-frozen technique is possible, supporting a method that aligns with the refinement principle of animals used in research.<\/jats:p>","DOI":"10.3390\/ani15060786","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T10:53:07Z","timestamp":1741690387000},"page":"786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison Between Two Methodologies of Sample Preservation for RNA Extraction in Naturally Delivered Ovine Placenta"],"prefix":"10.3390","volume":"15","author":[{"given":"Florencia","family":"Ar\u00e1nguiz","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-5740","authenticated-orcid":false,"given":"Javiera","family":"Bahamonde","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9197-8658","authenticated-orcid":false,"given":"Francisco","family":"Sales","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias, Centro Regional de Investigaci\u00f3n INIA Kampenaike, Punta Arenas 6200000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00edas","family":"Araya","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7750-0382","authenticated-orcid":false,"given":"C\u00e9sar","family":"Ulloa-Leal","sequence":"additional","affiliation":[{"name":"Escuela de Ciencias Agr\u00edcolas y Veterinarias, Universidad Vi\u00f1a del Mar, Vi\u00f1a del Mar 2520000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6072-7469","authenticated-orcid":false,"given":"Marcelo","family":"Ratto","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias, Centro Regional de Investigaci\u00f3n INIA Kampenaike, Punta Arenas 6200000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.vetmic.2008.09.054","article-title":"The ovine placenta and placentitis-A review","volume":"135","author":"Sammin","year":"2009","journal-title":"Vet. 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