{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T04:05:53Z","timestamp":1793505953455,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>A feeding trial was carried out using 576 Ross 308 male chickens to test the effects of an amorphous lignocellulose (LC) product (Arbocel). Three dietary treatments (control, 0.5% LC (LC 0.5), and 0.8% LC (LC 0.8) were used, and the diets were fed in eight replicate pens of 24 chickens. The LC treatments did not modify the weight gain of the chickens, but they tended to decrease the feed intake and FCR in the grower phase. The high swelling characteristics of LC resulted in lower dry matter (DM) contents in the crop (p &lt; 0.002) and gizzard (p &lt; 0.047) on day 14 and day 24, respectively. The lower pH in the gizzard and duodenum of LC diets suggests longer retention time and more HCl secretion in the proventriculus and gizzard. Except for \u03b1-amylase on day 24, the treatments did not modify the digestive enzyme content of the jejunum. The microbiota diversity and microbiota composition of ileal contents were affected only by the age of birds. The results suggest that the LC product can exert its potential positive effects mainly in the upper part of the digestive tract due to its special physical characteristics. LC can slow down the passage rate in the gizzard and modify the digestion and the dynamics of water absorption from the jejunum and ileum.<\/jats:p>","DOI":"10.3390\/ani15060851","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T11:04:22Z","timestamp":1742209462000},"page":"851","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Even Low Amounts of Amorphous Lignocellulose Affect Some Upper Gut Parameters, but They Do Not Modify Ileal Microbiota in Young Broiler Chickens"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4692-5813","authenticated-orcid":false,"given":"Val\u00e9ria","family":"Farkas","sequence":"first","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"Mayer","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Hungarian University of Agriculture and Life Sciences, Guba S\u00e1ndor Street 40, 7400 Kaposv\u00e1r, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Po\u00f3r","sequence":"additional","affiliation":[{"name":"Institute of Mathematics and Basic Science, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter P\u00e9tern\u00e9","family":"Farkas","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1976-2132","authenticated-orcid":false,"given":"Kesete Goitom","family":"Tewelde","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitta","family":"Kiss","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5182-7823","authenticated-orcid":false,"given":"Nikoletta","family":"Such","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9838-6703","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"P\u00e1l","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2002-0706","authenticated-orcid":false,"given":"G\u00e1bor","family":"Csit\u00e1ri","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-219X","authenticated-orcid":false,"given":"K\u00e1roly","family":"Dublecz","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Nutrition, Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, De\u00e1k Ferenc Street 16, 8360 Keszthely, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3382\/japr.2011-00477","article-title":"Poultry Response to High Levels of Dietary Fiber Sources Varying in Physical and Chemical Characteristics","volume":"21","author":"Mateos","year":"2012","journal-title":"J. Appl. Poult. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1093\/japr\/14.1.38","article-title":"Role of Insoluble Fiber on Gizzard Activity in Layers","volume":"14","author":"Hetland","year":"2005","journal-title":"J. Appl. Poult. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1017\/S0043933918000259","article-title":"Roles of Dietary Fibre and Ingredient Particle Size in Broiler Nutrition","volume":"74","author":"Kheravii","year":"2018","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1079\/BJN19960140","article-title":"The Influence of Dietary Fibre and Environmental Temoperature on the Development of the Gastrointestinal Tract, Digestibility, Degree of Fermentation in the Hind-Gut and Energy Metabolism in Pigs","volume":"75","author":"Zhao","year":"1996","journal-title":"Br. J. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1080\/00071669608417891","article-title":"Increased Small Intestinal Fermentation Is Partly Responsible for the Anti-nutritive Activity of Non-starch Polysaccharides in Chickens","volume":"37","author":"Choct","year":"1996","journal-title":"Br. Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e01437","DOI":"10.1016\/j.heliyon.2019.e01437","article-title":"An Update on Carbohydrases: Growth Performance and Intestinal Health of Poultry","volume":"5","author":"Raza","year":"2019","journal-title":"Heliyon"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1080\/10408398.2013.794327","article-title":"Utilization of Food Processing By-Products as Dietary, Functional, and Novel Fiber: A Review","volume":"56","author":"Sharma","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.foodres.2016.04.032","article-title":"Dietary Fibre: The Scientific Search for an Ideal Definition and Methodology of Analysis, and Its Physiological Importance as a Carrier of Bioactive Compounds","volume":"85","author":"Macagnan","year":"2016","journal-title":"Food Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0377-8401(03)00163-9","article-title":"A Review of Interactions between Dietary Fibre and the Intestinal Mucosa, and Their Consequences on Digestive Health in Young Non-Ruminant Animals","volume":"108","author":"Montagne","year":"2003","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7594","DOI":"10.1039\/C3CS60414D","article-title":"Catalytic Fast Pyrolysis of Lignocellulosic Biomass","volume":"43","author":"Liu","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5588","DOI":"10.1039\/c1cs15124j","article-title":"Catalytic Conversion of Lignocellulosic Biomass to Fine Chemicals and Fuels","volume":"40","author":"Zhou","year":"2011","journal-title":"Chem. Soc. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Waite, D.W., and Taylor, M.W. (2014). Characterizing the Avian Gut Microbiota: Membership, Driving Influences, and Potential Function. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00223"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.anifeedsci.2012.10.004","article-title":"Improving Digestive Utilization of Fiber-Rich Feedstuffs in Pigs and Poultry by Processing and Enzyme Technologies: A Review","volume":"178","author":"Pustjens","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_14","unstructured":"de Vries, S. (2018, January 12). Dietary Fiber in Poultry Nutrition: Dispensable Diluent or Pivotal for Digesta Transit Regulation?. Proceedings of the 17th BOKU-Symposium Animal Nutrition, Vienna, Austria."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"R\u00f6he, I., and Zentek, J. (2021). Lignocellulose as an Insoluble Fiber Source in Poultry Nutrition: A Review. J. Anim. Sci. Biotechnol., 12.","DOI":"10.1186\/s40104-021-00594-y"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3382\/ps\/pev336","article-title":"Effect of Insoluble Fiber Supplementation Applied at Different Ages on Digestive Organ Weight and Digestive Enzymes of Layer-Strain Poultry","volume":"95","author":"Yokhana","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_17","first-page":"531","article-title":"Effects of Insoluble Fiber on Growth Performance, Carcass Traits and Ileum Morphological Parameters on Broiler Chick Males","volume":"12","author":"Sarikhan","year":"2010","journal-title":"Int. J. Agric. Biol."},{"key":"ref_18","first-page":"83","article-title":"Performances, Ileal and Cecal Microbial Populations and Histological Characteristics in Broilers Fed Diets Supplemented with Lignocellulose","volume":"25","author":"Makivic","year":"2018","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.3382\/ps\/pex123","article-title":"Coarse Particle Inclusion and Lignocellulose-Rich Fiber Addition in Feed Benefit Performance and Health of Broiler Chickens","volume":"96","author":"Kheravii","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_20","unstructured":"Farran, M.T., Pietsch, M., and Chabrillat, T. (2013, January 26\u201328). Effect of Lignocellulose on the Litter Quality and the Ready to Cook Carcass Yield of Male Broilers. Proceedings of the Actes des 10\u00e8mes Journ\u00e9es de la Recherche Avicole et Palmip\u00e8des \u00e0 Foie Gras, France, Paris."},{"key":"ref_21","first-page":"212","article-title":"Ileal and Cecal Microbial Population and Short-Chain Fatty Acid Profile in Broiler Chickens Fed Diets Supplemented with Lignocellulose","volume":"35","author":"Szymeczko","year":"2015","journal-title":"Pak. Vet. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/1745039X.2018.1551950","article-title":"Impact of an Energy- and Nutrient-Reduced Diet Containing 10% Lignocellulose on Animal Performance, Body Composition and Egg Quality of Dual Purpose Laying Hens","volume":"73","author":"Urban","year":"2019","journal-title":"Arch. Anim. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3382\/ps\/pez492","article-title":"Effect of a \u201cDiluted\u201d Diet Containing 10% Lignocellulose on the Gastrointestinal Tract, Intestinal Microbiota, and Excreta Characteristics of Dual Purpose Laying Hens","volume":"99","author":"Vahjen","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_24","unstructured":"Aviagen (2025, February 03). Roos Broiler Management Handbook. Available online: www.aviagen.com."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bernfeld, P. (1955). Amylases, \u03b1 and \u03b2. Methods in Enzymology, Academic Press.","DOI":"10.1016\/0076-6879(55)01021-5"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/(SICI)1438-9312(200002)102:2<133::AID-EJLT133>3.0.CO;2-X","article-title":"Methods for Lipase Detection and Assay: Acritical Review","volume":"102","author":"Beisson","year":"2000","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_27","first-page":"515","article-title":"Enzymatic Assay of Trypsin","volume":"Volume 1","author":"Bergmeyer","year":"1974","journal-title":"Methods of Enzymatic Analysis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0003-2697(78)90334-2","article-title":"A Simplified Method of Quantitating Protein Using the Biuret and Phenol Reagents","volume":"86","author":"Ohnishi","year":"1978","journal-title":"Anal. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/nar\/gks808","article-title":"Evaluation of General 16S Ribosomal RNA Gene PCR Primers for Classical and Next-Generation Sequencing-Based Diversity Studies","volume":"41","author":"Klindworth","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Estaki, M., Jiang, L., Bokulich, N.A., McDonald, D., Gonz\u00e1lez, A., Kosciolek, T., Martino, C., Zhu, Q., Birmingham, A., and V\u00e1zquez-Baeza, Y. (2020). QIIME 2 Enables Comprehensive End-to-End Analysis of Diverse Microbiome Data and Comparative Studies with Publicly Available Data. Curr. Protoc. Bioinform., 70.","DOI":"10.1002\/cpbi.100"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/mSystems.00191-16","article-title":"Deblur Rapidly Resolves Single-Nucleotide Community Sequence Patterns","volume":"2","author":"Amir","year":"2017","journal-title":"mSystems"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA Ribosomal RNA Gene Database Project: Improved Data Processing and Web-Based Tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/s41596-019-0264-1","article-title":"Using MicrobiomeAnalyst for Comprehensive Statistical, Functional, and Meta-Analysis of Microbiome Data","volume":"15","author":"Chong","year":"2020","journal-title":"Nat. Protoc."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"C\u00e9lino, A., Fr\u00e9our, S., Jacquemin, F., and Casari, P. (2014). The Hygroscopic Behavior of Plant Fibers: A Review. Front. Chem., 1.","DOI":"10.3389\/fchem.2013.00043"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1080\/00071660120055331","article-title":"Effect of Oat Hulls on Performance, Gut Capacity and Feed Passage Time in Broiler Chickens","volume":"42","author":"Hetland","year":"2001","journal-title":"Br. Poult. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"623","DOI":"10.3382\/ps.0690623","article-title":"The Digestibility of Cell-Wall Polysaccharides from Wheat (Bran or Whole Grain), Soybean Meal, and White Lupin Meal in Cockerels, Muscovy Ducks, and Rats","volume":"69","author":"Carre","year":"1990","journal-title":"Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/0007166031000124595","article-title":"Effects of Oat Hulls and Wood Shavings on Digestion in Broilers and Layers Fed Diets Based on Whole or Ground Wheat","volume":"44","author":"Hetland","year":"2003","journal-title":"Br. Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103735","DOI":"10.1016\/j.psj.2024.103735","article-title":"Lignocellulose and Probiotic Supplementation in Broiler Chicken Diet: Effect on Growth Performance, Digestive Health, Litter Quality, and Genes Expression","volume":"103","author":"Soltan","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1080\/00071668.2018.1460461","article-title":"Body Growth, Intestinal Morphology and Microflora of Quail on Diets Supplemented with Micronised Wheat Fibre","volume":"59","author":"Rezaei","year":"2018","journal-title":"Br. Poult. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Jha, R., and Mishra, P. (2021). Dietary Fiber in Poultry Nutrition and Their Effects on Nutrient Utilization, Performance, Gut Health, and on the Environment: A Review. J. Anim. Sci. Biotechnol., 12.","DOI":"10.1186\/s40104-021-00576-0"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1093\/ps\/86.8.1705","article-title":"Effect of Type of Cereal, Heat Processing of the Cereal, and Inclusion of Fiber in the Diet on Productive Performance and Digestive Traits of Broilers","volume":"86","author":"Mateos","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1017\/S0043933911000249","article-title":"The Gizzard: Function, Influence of Diet Structure and Effects on Nutrient Availability","volume":"67","author":"Svihus","year":"2011","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"693","DOI":"10.3382\/ps.2011-01790","article-title":"The Effect of Insoluble Fiber and Intermittent Feeding on Gizzard Development, Gut Motility, and Performance of Broiler Chickens","volume":"91","author":"Sacranie","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.anifeedsci.2009.06.020","article-title":"Effects of Source of Fibre on the Development and PH of the Gastrointestinal Tract of Broilers","volume":"154","author":"Mateos","year":"2009","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Shang, Q., Wu, D., Liu, H., Mahfuz, S., and Piao, X. (2020). The Impact of Wheat Bran on the Morphology and Physiology of the Gastrointestinal Tract in Broiler Chickens. Animals, 10.","DOI":"10.3390\/ani10101831"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Such, N., Farkas, V., Csit\u00e1ri, G., P\u00e1l, L., M\u00e1rton, A., Menyh\u00e1rt, L., and Dublecz, K. (2021). Relative Effects of Dietary Administration of a Competitive Exclusion Culture and a Synbiotic Product, Age and Sampling Site on Intestinal Microbiota Maturation in Broiler Chickens. Vet. Sci., 8.","DOI":"10.3390\/vetsci8090187"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.3382\/ps\/pez090","article-title":"Amorphous Cellulose Feed Supplement Alters the Broiler Caecal Microbiome","volume":"98","author":"Eeckhaut","year":"2019","journal-title":"Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/6\/851\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:54:33Z","timestamp":1760028873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/6\/851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,16]]},"references-count":48,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["ani15060851"],"URL":"https:\/\/doi.org\/10.3390\/ani15060851","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,16]]}}}