{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:47:16Z","timestamp":1784648836214,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32160827"],"award-info":[{"award-number":["32160827"]}]},{"name":"National Natural Science Foundation of China","award":["20232BAB215057"],"award-info":[{"award-number":["20232BAB215057"]}]},{"name":"National Natural Science Foundation of China","award":["20232BAB215054"],"award-info":[{"award-number":["20232BAB215054"]}]},{"name":"National Natural Science Foundation of China","award":["JXSNKYJCRC202503"],"award-info":[{"award-number":["JXSNKYJCRC202503"]}]},{"name":"National Natural Science Foundation of China","award":["JXSNKYJCRC202217"],"award-info":[{"award-number":["JXSNKYJCRC202217"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["32160827"],"award-info":[{"award-number":["32160827"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["20232BAB215057"],"award-info":[{"award-number":["20232BAB215057"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["20232BAB215054"],"award-info":[{"award-number":["20232BAB215054"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["JXSNKYJCRC202503"],"award-info":[{"award-number":["JXSNKYJCRC202503"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["JXSNKYJCRC202217"],"award-info":[{"award-number":["JXSNKYJCRC202217"]}]},{"name":"Basic Research and Talent Development Program of Jiangxi Academy of Agricultural Sciences","award":["32160827"],"award-info":[{"award-number":["32160827"]}]},{"name":"Basic Research and Talent Development Program of Jiangxi Academy of Agricultural Sciences","award":["20232BAB215057"],"award-info":[{"award-number":["20232BAB215057"]}]},{"name":"Basic Research and Talent Development Program of Jiangxi Academy of Agricultural Sciences","award":["20232BAB215054"],"award-info":[{"award-number":["20232BAB215054"]}]},{"name":"Basic Research and Talent Development Program of Jiangxi Academy of Agricultural Sciences","award":["JXSNKYJCRC202503"],"award-info":[{"award-number":["JXSNKYJCRC202503"]}]},{"name":"Basic Research and Talent Development Program of Jiangxi Academy of Agricultural Sciences","award":["JXSNKYJCRC202217"],"award-info":[{"award-number":["JXSNKYJCRC202217"]}]},{"name":"Basic Research and Talent Training, Jiangxi Academy of Agricultural Sciences","award":["32160827"],"award-info":[{"award-number":["32160827"]}]},{"name":"Basic Research and Talent Training, Jiangxi Academy of Agricultural Sciences","award":["20232BAB215057"],"award-info":[{"award-number":["20232BAB215057"]}]},{"name":"Basic Research and Talent Training, Jiangxi Academy of Agricultural Sciences","award":["20232BAB215054"],"award-info":[{"award-number":["20232BAB215054"]}]},{"name":"Basic Research and Talent Training, Jiangxi Academy of Agricultural Sciences","award":["JXSNKYJCRC202503"],"award-info":[{"award-number":["JXSNKYJCRC202503"]}]},{"name":"Basic Research and Talent Training, Jiangxi Academy of Agricultural Sciences","award":["JXSNKYJCRC202217"],"award-info":[{"award-number":["JXSNKYJCRC202217"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The nonstructural protein 13 (NSP13) of PDCoV is a highly conservative helicase and plays key roles in viral replication. NSP13 contains a zinc-binding domain (ZBD), a helical Stalk domain, a beta-barrel 1B domain, and a core helicase domain. However, the specific functions of these domains of PDCoV NSP13 remain largely unknown. Here, we expressed and purified the wild-type NSP13WT and various mutants with domain deletions, and the activities of these proteins were analyzed using multiple methods. We found that NSP13\u0394ZBD possessed the abilities to hydrolyze ATP and unwind double-stranded nucleic acids, but the unwinding efficiency was lower than that of NSP13WT. In contrast, NSP13\u0394ZBD-Stalk, NSP13\u03941B, and NSP13\u0394ZBD-Stalk-1B all lost their unwinding activity, but not their ATPase activity. These results revealed that the deletion of the ZBD impaired the unwinding activity of PDCoV helicase NSP13, and the Stalk and 1B domains were critical for NSP13 to separate the duplexes. The identification of the roles of each domain in this study was helpful to gain an in-depth understanding of the overall functions of helicase NSP13, providing a theoretical basis for the development of antiviral drugs targeting helicase.<\/jats:p>","DOI":"10.3390\/ani15060865","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T06:02:40Z","timestamp":1742277760000},"page":"865","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Stalk and 1B Domains Are Required for Porcine Deltacoronavirus Helicase NSP13 to Separate the Double-Stranded Nucleic Acids, and the Deletion of the ZBD Impairs This Activity"],"prefix":"10.3390","volume":"15","author":[{"given":"Chengcheng","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihan","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanyong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5118-698X","authenticated-orcid":false,"given":"Fanfan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbing","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Duan, C. 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