{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:22:28Z","timestamp":1788852148550,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FONDEC 2021","award":["FNV-2021-05"],"award-info":[{"award-number":["FNV-2021-05"]}]},{"name":"FONDEC 2021","award":["FCN02614"],"award-info":[{"award-number":["FCN02614"]}]},{"name":"FOPER 2022","award":["FNV-2021-05"],"award-info":[{"award-number":["FNV-2021-05"]}]},{"name":"FOPER 2022","award":["FCN02614"],"award-info":[{"award-number":["FCN02614"]}]},{"name":"University Autonomous of Quer\u00e9taro","award":["FNV-2021-05"],"award-info":[{"award-number":["FNV-2021-05"]}]},{"name":"University Autonomous of Quer\u00e9taro","award":["FCN02614"],"award-info":[{"award-number":["FCN02614"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Many countries have banned the use of antibiotics in livestock feed due to the development of antibiotic-resistant bacteria. Therefore, it is necessary to identify suitable alternatives to antibiotics in order to maintain intestinal health and improve piglet performance after weaning. The objective of this study was to evaluate how the intake of two functional foods\u2014potato protein concentrate (PP) and Saccharomyces boulardii (Sb), either isolated or combined\u2014would affect productive performance and some digestive morphophysiological responses after two weeks post-weaning. We evaluated five diets: SB, with Sb; PPC, with PP; PPC-SB, with a combination of PP and Sb; C\u2212, without antibiotics, Sb, or PP; and C+, with antibiotics. Neither the absence of antibiotics in the diet nor the inclusion of PP and Sb affected performance or digestive organ development during the post-weaning phase. Piglets fed the C+ diet and the PP-Sb combination had higher concentrations of occludin proteins and longer villi in the jejunum compared to those fed C\u2212, PPC, and SB. The use of Sb alone or in combination with PP increased fecal Lactobacillus, while antibiotics reduced coliform bacteria. The PP-Sb combination prevented the atrophy of the jejunal villi and promoted adhesion between enterocytes in the jejunum. Including these functional foods in piglet diets contributed to maintaining intestinal health during the critical post-weaning phase. However, further research is needed to validate and enhance the conclusions of this study.<\/jats:p>","DOI":"10.3390\/ani15070985","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T04:21:01Z","timestamp":1743481261000},"page":"985","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Supplementation with Potato Protein Concentrate and Saccharomyces boulardii to an Antibiotic-Free Diet Improves Intestinal Health in Weaned Piglets"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9025-4332","authenticated-orcid":false,"given":"T\u00e9rcia Ces\u00e1ria","family":"Reis de Souza","sequence":"first","affiliation":[{"name":"Faculty of Natural Sciences, Autonomous University of Quer\u00e9taro, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6684-4765","authenticated-orcid":false,"given":"Gerardo Mariscal","family":"Land\u00edn","sequence":"additional","affiliation":[{"name":"National Institute of Agricultural and Livestock Forestry Research, National Center of Research in Animal Physiology, Ajuchitl\u00e1n Col\u00f3n, Quer\u00e9taro 76280, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3751-6601","authenticated-orcid":false,"given":"Ulisses Moreno","family":"Celis","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Autonomous University of Quer\u00e9taro, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresita Hijuitl","family":"Valeriano","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Autonomous University of Quer\u00e9taro, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8837-3578","authenticated-orcid":false,"given":"Jos\u00e9 Guadalupe","family":"G\u00f3mez-Soto","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Autonomous University of Quer\u00e9taro, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Narv\u00e1ez","family":"Briones","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Autonomous University of Quer\u00e9taro, Quer\u00e9taro 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"G927","DOI":"10.1152\/ajpgi.00206.2015","article-title":"Early-life stress Origins of gastrointestinal disease: Animal models, intestinal pathophysiology, and translational implications","volume":"309","author":"Pohl","year":"2015","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.2527\/jas.2015-9767","article-title":"Invited review: Aspects of gastrointestinal tract growth and maturation in the pre-and postweaning period of pigs","volume":"94","author":"Pluske","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_3","first-page":"541","article-title":"Impact of stress on the functioning of the immune system, swine health, and productivity","volume":"78","author":"Pejsak","year":"2022","journal-title":"Med. Weter."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tang, X., Xiong, K., Fang, R., and Li, M. (2022). Weaning stress and intestinal health of piglets: A review. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1042778"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13028-017-0299-7","article-title":"Post weaning diarrhea in pigs: Risk factors and non-colistin-based control strategies","volume":"59","author":"Rhouma","year":"2017","journal-title":"Acta Vet. Scand."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"114727","DOI":"10.1016\/j.anifeedsci.2020.114727","article-title":"Alternatives to antibiotics and trace elements (copper and zinc) to improve gut health and zootechnical parameters in piglets: A review","volume":"271","author":"Pechova","year":"2021","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Liu, H.Y., Zhu, C., Zhu, M., Yuan, L., Li, S., Gu, F., Hu, P., Chen, S., and Cai, D. (2024). Alternatives to antibiotics in pig production: Looking through the lens of immunophysiology. Stress Biol., 4.","DOI":"10.1007\/s44154-023-00134-w"},{"key":"ref_8","first-page":"159","article-title":"Growth performance, diarrhoea incidence, and nutrient digestibility in weaned piglets fed an antibiotic-free diet with dehydrated porcine plasma or potato protein concentrate","volume":"191","author":"Barreyro","year":"2019","journal-title":"Ann. Anim. Sci."},{"key":"ref_9","first-page":"510","article-title":"Concentrado de prote\u00edna de papa: Una posible alternativa al uso de antibi\u00f3ticos en las dietas para lechones destetados. Revisi\u00f3n","volume":"132","year":"2022","journal-title":"Rev. Mex. Cienc. Pecu."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s12230-018-09705-4","article-title":"Potatoes, Nutrition and Health","volume":"96","author":"Beals","year":"2019","journal-title":"Am. J. Potato Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1111\/ijfs.14343","article-title":"Towards potato protein utilisation: Insights into separation, functionality and bioactivity of patatin","volume":"55","author":"Fu","year":"2020","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6564","DOI":"10.1080\/10408398.2022.2036093","article-title":"Potato protein: Current review of structure, technological properties, and potential application on spray drying microencapsulation","volume":"6323","author":"Galves","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"110415","DOI":"10.1016\/j.foodhyd.2024.110415","article-title":"Potato protein as an emerging high-quality: Source, extraction, purification, properties functional, nutritional, physicochemical, and processing, applications, and challenges using potato protein","volume":"157","author":"Bhutto","year":"2024","journal-title":"Food Hydrocoll."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5533","DOI":"10.1007\/s00253-019-09887-9","article-title":"Antifungal and antimicrobial proteins and peptides of potato Solanum tuberosum L. tubers and their applications","volume":"103","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.copbio.2017.09.007","article-title":"Shared mechanisms among probiotic taxa: Implications for general probiotic claims","volume":"49","author":"Sanders","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Fijan, S. (2023). Probiotics and their antimicrobial effect. Microorganisms, 11.","DOI":"10.3390\/books978-3-0365-6932-1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1080\/1828051X.2021.1952909","article-title":"The effect of a single, early-life administration of a probiotic on piglet growth performance and faecal microbiota until weaning","volume":"20","author":"Luise","year":"2021","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Pang, Y., Zhang, H., Wen, H., Wan, H., Wu, H., Chen, Y., and Liu, X. (2022). Yeast probiotic and yeast products in enhancing livestock feeds utilization and performance: An overview. J. Fungi, 8.","DOI":"10.3390\/jof8111191"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.14202\/vetworld.2023.1035-1042","article-title":"In-feed nutritional additive probiotic Saccharomyces boulardii RC009 can substitute for prophylactic antibiotics and improve the production and health of weaning pigs","volume":"165","author":"Parada","year":"2023","journal-title":"Vet. World"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Alkalbani, N.S., Osaili, T.M., Al-Nabulsi, A.A., Olaimat, A.N., Liu, S.Q., Shah, N.P., Apostolopoulos, V., and Ayyash, M.M. (2022). Assessment of yeasts as potential probiotics: A review of gastrointestinal tract conditions and investigation methods. J. Fungi, 8.","DOI":"10.3390\/jof8040365"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/s41575-023-00766-3","article-title":"Paracellular permeability and tight junction regulation in gut health and disease","volume":"20","author":"Horowitz","year":"2023","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Xiong, X., Tan, B., Song, M., Ji, P., Kim, K., Yin, Y., and Liu, Y. (2019). Nutritional intervention for the intestinal development and health of weaned pigs. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00046"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Robinson, K., Deng, Z., Hou, Y., and Zhang, G. (2015). Regulation of the intestinal barrier function by host defense peptides. Front. Vet. Sci., 2.","DOI":"10.3389\/fvets.2015.00057"},{"key":"ref_24","unstructured":"World Health Organization (1985). International Guiding Principles for Biomedical Research Involving Animals. International Guiding Principles for Biomedical Research Involving Animals, Council for International Organization of Medical Sciences."},{"key":"ref_25","unstructured":"(2024, August 09). Diario Oficial de la Federaci\u00f3n. 22 August. Available online: http:\/\/legismex.mty.itesm.mx\/normas\/zoo\/zoo062.pdf."},{"key":"ref_26","unstructured":"NRC (2012). Nutrient Requirements of Swine, National Academic Press. [11th ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Schubert, D.C., M\u00f6\u00dfeler, A., Ahlf\u00e4nger, B., Langeheine, M., Brehm, R., Visscher, C., El-Wahab, A.A., and Kamphues, J. (2022). Influences of exocrine pancreatic insufficiency on nutrient digestibility, growth parameters as well as anatomical and histological morphology of the intestine in a juvenile pig model. Front. Med., 9.","DOI":"10.3389\/fmed.2022.973589"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kurz, A., and Seifert, J. (2021). Factors influencing proteolysis and protein utilization in the intestine of pigs: A review. Animals, 11.","DOI":"10.3390\/ani11123551"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.aninu.2021.08.001","article-title":"Fate of undigested proteins in the pig large intestine: What impact on the colon epithelium?","volume":"9","author":"Blachier","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_30","first-page":"353","article-title":"Antibiotic-free diet supplemented with live yeasts decreases inflammatory markers in the ileum of weaned piglets","volume":"503","year":"2020","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_31","unstructured":"Diario Oficial de la Federaci\u00f3n (2024, August 09). NOM-113-SSA1-Bienes y Servicios. C. 25 August. Available online: http:\/\/www.ordenjuridico.gob.mx\/Documentos\/Federal\/wo69536.pdf."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1365-2672.1960.tb00188.x","article-title":"A Medium for the Cultivation of Lactobacilli","volume":"23","author":"Rogosa","year":"1960","journal-title":"J. Appl. Bacteriol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0301-6226(97)00057-2","article-title":"Factors influencing the structure and function of the small intestine in the weaned pig: A review","volume":"511\u2013513","author":"Pluske","year":"1997","journal-title":"Livest. Prod. Sci."},{"key":"ref_34","first-page":"63","article-title":"Efecto del nivel de taninos del sorgo y del d\u00eda posdestete sobre algunas caracter\u00edsticas morfofisiol\u00f3gicas del aparato digestivo de lechones","volume":"23","author":"Aguilera","year":"2015","journal-title":"Arch. Latinoam. Prod. Anim."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.2527\/jas2017.1868","article-title":"Poorer lifetime growth performance of gilt progeny compared with sow progeny is largely due to weight differences at birth and reduced growth in the preweaning period, and is not improved by progeny segregation after weaning","volume":"95","author":"Craig","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.aninu.2021.11.006","article-title":"The developmental changes in intestinal epithelial cell proliferation, differentiation, and shedding in weaning piglets","volume":"9","author":"Wang","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Yang, F., Wang, A., Zeng, X., Hou, C., Liu, H., and Qiao, S. (2015). Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions. BMC Microbiol., 151.","DOI":"10.1186\/s12866-015-0372-1"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"104058","DOI":"10.1016\/j.jff.2020.104058","article-title":"Supplementation with yeast culture improves the integrity of intestinal tight junction proteins via NOD1\/NF-\u039aB P65 pathway in weaned piglets and H2O2-challenged IPEC-J2 cells","volume":"72","author":"Wang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.aninu.2017.06.002","article-title":"Husbandry practices and gut health outcomes in weaned pigs: A review","volume":"3","author":"Jayaraman","year":"2017","journal-title":"Anim. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"jcs261776","DOI":"10.1242\/jcs.261776","article-title":"A short guide to the tight junction","volume":"137","author":"Citi","year":"2024","journal-title":"J. Cell Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1996830","DOI":"10.1080\/21688370.2021.1996830","article-title":"Epithelial integrity, junctional complexes, and biomarkers associated with intestinal functions","volume":"10","author":"Alizadeh","year":"2022","journal-title":"Tissue Barriers"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Modina, S.C., Aidos, L., Rossi, R., Pocar, P., Corino, C., and Di Giancamillo, A. (2021). Stages of gut development as a useful tool to prevent gut alterations in piglets. Animals, 11.","DOI":"10.3390\/ani11051412"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Rodrigues, L.A., Koo, B., Nyachoti, M., and Columbus, D.A. (2022). Formulating diets for improved health status of pigs: Current knowledge and perspectives. Animals, 12.","DOI":"10.3390\/ani12202877"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sampath, V., Heon Baek, D., Shanmugam, S., and Kim, I.H. (2021). Dietary inclusion of blood plasma with yeast Saccharomyces cerevisiae supplementation enhanced the growth performance, nutrient digestibility, Lactobacillus count, and reduced gas emissions in weaning pigs. Animals, 113.","DOI":"10.3390\/ani11030759"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Parada, J., Magnoli, A., Poloni, V., Corti Isgro, M., Rosales Cavaglieri, L., Luna, M.J., Carranza, A., and Cavaglieri, L. (2024). Pediococcus pentosaceus RC007 and Saccharomyces boulardii RC009 as antibiotic alternatives for gut health in post-weaning pigs. J. Appl. Microbiol., 135.","DOI":"10.1093\/jambio\/lxae282"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Gresse, R., Chaucheyras Durand, F., Duni\u00e8re, L., Blanquet-Diot, S., and Forano, E. (2019). Microbiota composition and functional profiling throughout the gastrointestinal tract of commercial weaning piglets. Microorganisms, 7.","DOI":"10.3390\/microorganisms7090343"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zhang, W., Bao, C., Wang, J., Zang, J., and Cao, Y. (2020). Administration of Saccharomyces boulardii mafic-1701 improves feed conversion ratio, promotes antioxidant capacity, alleviates intestinal inflammation and modulates gut microbiota in weaned piglets. J. Anim. Sci. Biotechnol., 11.","DOI":"10.1186\/s40104-020-00516-4"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"110583","DOI":"10.1016\/j.foodres.2021.110583","article-title":"Potato protein: An emerging source of high quality and allergy free protein, and its possible future based products","volume":"148","author":"Hussain","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"103533","DOI":"10.1016\/j.ifset.2023.103533","article-title":"Potato proteins for technical applications: Nutrition, isolation, modification and functional properties\u2014A review","volume":"91","author":"Hu","year":"2024","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tang, J., Zhao, M., Yang, W., Chen, H., Dong, Y., He, Q., Miao, X., and Zhang, J. (2024). Effect of composite probiotics on antioxidant capacity, gut barrier functions, and fecal microbiome of weaned piglets and sows. Animals, 14.","DOI":"10.3390\/ani14091359"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1749-6632.2012.06553.x","article-title":"Microbial butyrate and its role for barrier function in the gastrointestinal tract","volume":"1258","author":"Stumpff","year":"2012","journal-title":"Ann. N. Y. Acad. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/7\/985\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:05:09Z","timestamp":1760029509000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/7\/985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,29]]},"references-count":51,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["ani15070985"],"URL":"https:\/\/doi.org\/10.3390\/ani15070985","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,29]]}}}