{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:09:25Z","timestamp":1768284565586,"version":"3.49.0"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","award":["FF.KU 7.66"],"award-info":[{"award-number":["FF.KU 7.66"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]},{"name":"International SciKU Branding (ISB), Faculty of Science, Kasetsart University, Thailand","award":["FF.KU 7.66"],"award-info":[{"award-number":["FF.KU 7.66"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The gut microbiota is pivotal to chickens\u2019 overall health, influencing digestion, nutrient absorption, and immune function. Dietary compounds significantly impact gut microbiota composition. House crickets (Acheta domesticus) have emerged as an alternative protein source for animal feed, rich in proteins and beneficial fatty acids. This study compared the gut microbiota in the cecum and ileum of Thai indigenous chicken breeds (Betong Chicken, white feather with black bone chicken, and black feather with black bone chicken) fed with or without house crickets. Using Oxford Nanopore Technology of 16S rDNA, this study found a similar relative abundance of gut bacteria across groups, with dominant bacteria including Firmicute, Bacteroidetes, Proteobacteria, and Actinobacteria. LEfSe analysis identified differential abundance of beneficial bacteria, such as Ruminococcaceae, Rikenella, and Deferribacteres, in the cecum of the black feather with black bone chicken after cricket feeding. Additionally, Lactobacillaceae exhibited differential abundance in the ileum of this breed post-cricket diet. Consequently, this study provides new data into the gut microbiota of Thai indigenous chickens. It suggests that house cricket diets did not significantly alter microbiota diversity but may enhance beneficial bacteria in certain breeds.<\/jats:p>","DOI":"10.3390\/ani15071070","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:59:00Z","timestamp":1744091940000},"page":"1070","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Analysis of the Gut Microbiota of Thai Indigenous Chicken Fed House Crickets"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3520-8330","authenticated-orcid":false,"given":"Nattanan Panjaworayan","family":"T-Thienprasert","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Titiradsadakorn","family":"Jaithon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8383-9536","authenticated-orcid":false,"given":"Pavit","family":"Klomkliew","sequence":"additional","affiliation":[{"name":"Center of Excellence in Systems Microbiology, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prangwalai","family":"Chanchaem","sequence":"additional","affiliation":[{"name":"Center of Excellence in Systems Microbiology, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9707-6428","authenticated-orcid":false,"given":"Thanathip","family":"Suwanasopee","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6170-7876","authenticated-orcid":false,"given":"Skorn","family":"Koonawootrittriron","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0769-5234","authenticated-orcid":false,"given":"Attawit","family":"Kovitvadhi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0159-0869","authenticated-orcid":false,"given":"Pipatpong","family":"Chundang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prapasiri","family":"Pongprayoon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8557-6655","authenticated-orcid":false,"given":"Sutasinee","family":"Kityakarn","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patraporn","family":"Luksirikul","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunchai","family":"Payungporn","sequence":"additional","affiliation":[{"name":"Center of Excellence in Systems Microbiology, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"ref_1","first-page":"5304028","article-title":"The Microbiome of Animals: Implications for Conservation Biology","volume":"2016","author":"Bahrndorff","year":"2016","journal-title":"Int. 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