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and technology plan of Yangjiang City","award":["2021B0202020002"],"award-info":[{"award-number":["2021B0202020002"]}]},{"name":"science and technology plan of Yangjiang City","award":["32273131"],"award-info":[{"award-number":["32273131"]}]},{"name":"science and technology plan of Yangjiang City","award":["2023B0202010016"],"award-info":[{"award-number":["2023B0202010016"]}]},{"name":"science and technology plan of Yangjiang City","award":["2023TQ07A888"],"award-info":[{"award-number":["2023TQ07A888"]}]},{"name":"science and technology plan of Yangjiang City","award":["2024E03007"],"award-info":[{"award-number":["2024E03007"]}]},{"name":"science and technology plan of Yangjiang City","award":["2022011"],"award-info":[{"award-number":["2022011"]}]},{"name":"science and technology plan of Yangjiang City","award":["SDZX2023027"],"award-info":[{"award-number":["SDZX2023027"]}]},{"name":"science and technology plan of Yangjiang City","award":["BQW2024013"],"award-info":[{"award-number":["BQW2024013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Insulin-like growth factors (IGFs) play crucial roles in the regulation of animal growth and reproduction. However, the functional and regulatory mechanisms underlying ovarian growth and oocyte maturation in teleosts remain unclear. In this study, the expression profiles of lncRNAs and mRNAs were analyzed in the ovaries of golden pompano (Trachinotus ovatus) treated with IGF1 and IGF2 proteins to gain insights into the role of these two IGF ligands in the regulation of ovarian development and maturation. A total of 1494 lncRNAs and 8728 mRNAs were differentially expressed following IGF1 treatment compared with the control group. A total of 101 lncRNAs and 377 mRNAs were differentially expressed after IGF2 treatment compared to those in the control group. The results revealed that KEGG pathways enriched by target genes of the DE lncRNAs overlapped significantly with those enriched by the DE mRNAs in both the IGF1 and IGF2 groups. The key overlapping enriched pathways included ECM receptor interaction, gap junction, Hedgehog signaling pathway, Ras signaling pathway, Rap1 signaling pathway, TGF beta signaling pathway, Wnt signaling pathway, GnRH signaling pathway, progesterone-mediated oocyte maturation, oocyte meiosis, cell cycle, and MAPK signaling pathway. The differentially expressed genes (DEGs) involved in ovarian development and oocyte maturation were cyp17a1, cyp19a1, star, hsd17b3, hsd17b7, adam23, slc26a6, htr2b, h2ax, nanos3, krt18, pgr, and inhbb, following IGF1 and IGF2 treatment. Furthermore, four lncRNAs (MSTRG.66521.1, MSTRG.49969.1, MSTRG.59923.1, and MSTRG.13767.1) for IGF1 and two (MSTRG.20896.2 and MSTRG.58123.2) for IGF2 within the lncRNA\u2013mRNA network were found to target DEGs related to ovarian development and maturation. This suggests that IGFs may affect reproductive processes by regulating the expression of lncRNAs and mRNAs. RT-qPCR analysis revealed that these six lncRNAs showed high expression levels in the brain, pituitary, liver, and gonad tissues, indicating their potential involvement in regulating ovarian growth and development. This study elucidates the lncRNA\u2013mRNA regulatory mechanism in response to IGF1 and IGF2 treatment during stage III of ovarian development in golden pompano, thereby deepening our understanding of its functional role.<\/jats:p>","DOI":"10.3390\/ani15081134","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T06:50:27Z","timestamp":1744699827000},"page":"1134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Integrated lncRNA and mRNA Transcriptome Analyses of IGF1 and IGF2 Stimulated Ovaries Reveal Genes and Pathways Potentially Associated with Ovarian Development and Oocyte Maturation in Golden Pompano (Trachinotus ovatus)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5000-5112","authenticated-orcid":false,"given":"Charles Brighton","family":"Ndandala","sequence":"first","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0576-5657","authenticated-orcid":false,"given":"Yuwen","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Ju","sequence":"additional","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Fachri","sequence":"additional","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Happiness Moses","family":"Mwemi","sequence":"additional","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huapu","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.theriogenology.2017.08.027","article-title":"Transcriptome Analysis of Three Critical Periods of Ovarian Development in Yellow River Carp (Cyprinus carpio)","volume":"105","author":"Jia","year":"2018","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100866","DOI":"10.1016\/j.aqrep.2021.100866","article-title":"Identification and Ovarian Developmental Regulation of Insulin-like Growth Factor 3 in Spotted Scat (Scatophagus argus)","volume":"21","author":"Li","year":"2021","journal-title":"Aquac. 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