{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T14:58:08Z","timestamp":1788101888011,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Plan Nacional de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID 2020-113221RB-I00"],"award-info":[{"award-number":["PID 2020-113221RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Plan Nacional de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2023-149326OB-C21"],"award-info":[{"award-number":["PID2023-149326OB-C21"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Plan Nacional de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["RYC 2019-026841-I"],"award-info":[{"award-number":["RYC 2019-026841-I"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ram\u00f3n y Cajal contract","award":["PID 2020-113221RB-I00"],"award-info":[{"award-number":["PID 2020-113221RB-I00"]}]},{"name":"Ram\u00f3n y Cajal contract","award":["PID2023-149326OB-C21"],"award-info":[{"award-number":["PID2023-149326OB-C21"]}]},{"name":"Ram\u00f3n y Cajal contract","award":["RYC 2019-026841-I"],"award-info":[{"award-number":["RYC 2019-026841-I"]}]},{"name":"government of Spain","award":["PID 2020-113221RB-I00"],"award-info":[{"award-number":["PID 2020-113221RB-I00"]}]},{"name":"government of Spain","award":["PID2023-149326OB-C21"],"award-info":[{"award-number":["PID2023-149326OB-C21"]}]},{"name":"government of Spain","award":["RYC 2019-026841-I"],"award-info":[{"award-number":["RYC 2019-026841-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster and more reliable results. The objective of this study was to validate the Sysmex XN-1000V automatic hematology analyzer for blood samples from rainbow trout (Oncorhynchus mykiss), examine the effects of two anticoagulants (K2EDTA and lithium heparin), and establish normal blood reference values for this fish species. Additionally, comparative studies were conducted between the Sysmex XN-1000V and manual methods (hemocytometer cell count and blood smear estimation), and reference intervals were established. Ninety-nine heparinized blood samples were analyzed for validation and sample stability tests. The results showed extremely good precision, with a coefficient of variation (CV) below 3% for RBCs, HGB, and HCT and less than 5% for non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, with a CV of 15.08%. Linearity was excellent, and the carry-over was below 1% for all parameters. The sample stability test indicated that samples could be analyzed for up to 48 h when stored at 4 \u00b0C and up to 24 h at room temperature. Non-RBC cells were the first to degrade over time. The automated and manual methods demonstrated good correlation and agreement, validating the analyzer\u2019s accuracy. The effects of two anticoagulants, K2EDTA and lithium heparin, on the blood samples were also studied. Heparin was the preferred anticoagulant for routine hematological analysis of rainbow trout blood with the Sysmex XN-1000V analyzer. In conclusion, the Sysmex XN-1000V enables complete hemogram analyses to be performed quickly and accurately, standardizing techniques, harmonizing results, and providing reliable reference intervals with O mykiss blood.<\/jats:p>","DOI":"10.3390\/ani15091265","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T11:00:54Z","timestamp":1745924454000},"page":"1265","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preliminary Evaluation of an Automated Blood Cell Analyzer for Its Use with Blood Samples from Rainbow Trout Oncorhynchus mykiss"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2969-9172","authenticated-orcid":false,"given":"Montse","family":"Mesalles","sequence":"first","affiliation":[{"name":"Servei d\u2019Hematologia Cl\u00ednica Veterin\u00e0ria (SHCV), Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meritxell","family":"Uroz","sequence":"additional","affiliation":[{"name":"Departament de Biologia Celular, Fisiologia i Immunologia, Facultat de Biociencies, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7372-8639","authenticated-orcid":false,"given":"Irene","family":"Brandts","sequence":"additional","affiliation":[{"name":"Departament de Biologia Celular, Fisiologia i Immunologia, Facultat de Biociencies, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9799-9804","authenticated-orcid":false,"given":"Emmanuel","family":"Serrano","sequence":"additional","affiliation":[{"name":"Wildlife Ecology & Health Group (WE&H), Servei d\u2019Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-8512","authenticated-orcid":false,"given":"Rafaela","family":"Cuenca","sequence":"additional","affiliation":[{"name":"Servei d\u2019Hematologia Cl\u00ednica Veterin\u00e0ria (SHCV), Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1702-9531","authenticated-orcid":false,"given":"Josep","family":"Pastor","sequence":"additional","affiliation":[{"name":"Servei d\u2019Hematologia Cl\u00ednica Veterin\u00e0ria (SHCV), Departament de Medicina i Cirurgia Animals, Facultat de Veterin\u00e0ria, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5525-4049","authenticated-orcid":false,"given":"Mariana","family":"Teles","sequence":"additional","affiliation":[{"name":"Departament de Biologia Celular, Fisiologia i Immunologia, Facultat de Biociencies, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Cerdanyola del Vall\u00e8s, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/j.1095-8649.1973.tb04510.x","article-title":"Routine hematological methods for use with fish blood","volume":"5","author":"Blaxhall","year":"1973","journal-title":"J. Fish Biol."},{"key":"ref_2","first-page":"191","article-title":"Hematological evaluation of welfare and health of fish","volume":"53","author":"Ivanc","year":"2005","journal-title":"Praxis Vet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.cvex.2008.03.007","article-title":"Hematologic disorders of fish","volume":"11","author":"Clauss","year":"2008","journal-title":"Vet. Clin. N. Am. Exot. Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.cvex.2014.09.007","article-title":"Fish hematology and associated disorders","volume":"18","author":"Grant","year":"2015","journal-title":"Vet. Clin. N. Am. Exot. Anim. Pract."},{"key":"ref_5","first-page":"317","article-title":"Use of hematological parameters as assessment tools in fish health status","volume":"24","author":"Docan","year":"2018","journal-title":"J. Agroaliment. Proces Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1007\/s10661-022-10250-1","article-title":"The role of sex, season and reproduction status on blood parameters in snow trout (Schizothorax labiatus) from River Jhelum, Kashmir, India","volume":"194","author":"Jan","year":"2022","journal-title":"Environ. Monit. Assess."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"737498","DOI":"10.1016\/j.aquaculture.2021.737498","article-title":"Hematological methods in fish: Not only for beginners","volume":"547","author":"Witeska","year":"2022","journal-title":"Aquaculture"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"734398","DOI":"10.1016\/j.aquaculture.2019.734398","article-title":"Flow cytometry and automatic blood cell analysis in striped bass Morone saxatilis (Walbaum, 1792): A new hematological approach","volume":"513","author":"Fazio","year":"2019","journal-title":"Aquaculture"},{"key":"ref_9","unstructured":"Food and Agriculture Organization of the United Nations (FAO) (2024). The State of World Fisheries and Aquaculture 2024: Blue Transformation in Action, FAO."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"156910","DOI":"10.1016\/j.scitotenv.2022.156910","article-title":"Blood biomarkers as diagnostic tools: An overview of climate-driven stress responses in fish","volume":"843","author":"Shahjahan","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/10236244.2013.817728","article-title":"Influence of different salinity on hematological and biochemical parameters of the widely cultured mullet, Mugil cephalus","volume":"46","author":"Fazio","year":"2013","journal-title":"Mar. Freshw. Behav. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s10228-012-0303-1","article-title":"Hematological and serum protein profiles of Mugil cephalus: Effect of two different habitats","volume":"60","author":"Fazio","year":"2013","journal-title":"Ichthyol. Res."},{"key":"ref_13","first-page":"114","article-title":"Investigation of certain blood parameters in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) naturally infected with Lactococcus garvieae","volume":"8","author":"Avsever","year":"2014","journal-title":"J. Fish. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.fsi.2018.04.034","article-title":"Trans-cinnamic acid application for rainbow trout (Oncorhynchus mykiss): I. Effects on hematological, serum biochemical, non-specific immune and head kidney gene expression responses","volume":"78","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"ref_15","first-page":"252","article-title":"Automatic analysis to assess hematological parameters in farmed gilthead sea bream (Sparus aurata)","volume":"364\u2013365","author":"Fazio","year":"2012","journal-title":"Aquaculture"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.cbpc.2007.04.004","article-title":"The effects of anticoagulants on hematological indices and blood cell morphology of common carp (Cyprinus carpio L.)","volume":"146","author":"Walencik","year":"2007","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/24750263.2018.1460694","article-title":"Comparative study of haematology of two teleost fish (Mugil cephalus and Carassius auratus) from different environments and feeding habits","volume":"85","author":"Parrino","year":"2018","journal-title":"Eur. Zool. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3906\/vet-1611-35","article-title":"The effects of heparin concentration, storage time and temperature on the values of hematological parameters in Cyprinus carpio","volume":"41","author":"Witeska","year":"2017","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_19","first-page":"615","article-title":"Effect of three different anticoagulants and storage time on hematological parameters of Mugil cephalus (Linnaeus, 1758)","volume":"14","author":"Faggio","year":"2014","journal-title":"Turk. J. Fish. Aqua. Sci."},{"key":"ref_20","first-page":"94","article-title":"Leukocyte count in fish: Possible sources of discrepancy","volume":"37","author":"Lugowska","year":"2017","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"ref_21","first-page":"234","article-title":"Differences in hematological values of common carp between cardiac and venous blood","volume":"38","author":"Bojarski","year":"2018","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Carbajal, A., Soler, P., Tallo-Parra, O., Isasa, M., Echevarria, C., Lopez-Bejar, M., and Vinyoles, D. (2019). Towards non-invasive methods in measuring fish welfare: The measurement of cortisol concentrations in fish skin mucus as a biomarker of habitat quality. Animals, 9.","DOI":"10.3390\/ani9110939"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1007\/s00580-022-03375-9","article-title":"Impact of three anticoagulants and their storage time on hematological parameters of snow trout, Schizothorax labiatus, habiting in river Sindh of Indian Himalayan region","volume":"31","author":"Jan","year":"2022","journal-title":"Comp. Clin. Pathol."},{"key":"ref_24","first-page":"213","article-title":"Comparative study of hematological profile on Gobius niger in two different habitat sites: Faro Lake and Tyrrhenian Sea","volume":"53","author":"Fazio","year":"2012","journal-title":"Cah. Biol. Mar."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.aquaculture.2018.10.030","article-title":"Fish hematology analysis as an important tool of aquaculture: A review","volume":"500","author":"Fazio","year":"2019","journal-title":"Aquaculture"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/raq.12753","article-title":"Application of hematology parameters for health management in fish farms","volume":"15","author":"Chen","year":"2023","journal-title":"Rev. Aquac."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1139\/f83-060","article-title":"Normal ranges for diagnostically important hematological and blood chemistry characteristics of rainbow trout (Salmo gairdneri)","volume":"40","author":"Miller","year":"1983","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"ref_28","unstructured":"Wedemeyer, G.A., and Yasutake, W.T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health, Technical Paper."},{"key":"ref_29","unstructured":"Weiss, D.J., and Wardrop, K.J. (2010). Hematology of fishes. Schalm\u2019s Veterinary Hematology, Wiley-Blackwell."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/j.1939-165X.2006.tb00160.x","article-title":"Hematology and plasma chemistry reference intervals for cultured shortnose sturgeon (Acipenser brevirostrum)","volume":"35","author":"Knowles","year":"2006","journal-title":"Vet. Clin. Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.1016\/j.sjbs.2022.01.019","article-title":"Hematological and serum biochemical reference intervals of rainbow trout (Oncorhynchus mykiss) cultured in Himalayan aquaculture: Morphology, morphometrics and quantification of peripheral blood cells","volume":"29","author":"Nabi","year":"2022","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/vcp.12006","article-title":"ASVCP reference interval guidelines: Determination of de novo reference intervals in veterinary species and other related topics","volume":"41","author":"Friedrichs","year":"2012","journal-title":"Vet. Clin. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/vcp.12810","article-title":"ASVCP guidelines: Principles of quality assurance and standards for veterinary clinical pathology (version 3.0)","volume":"48","author":"Arnold","year":"2019","journal-title":"Vet. Clin. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1046\/j.1365-2109.2003.00916.x","article-title":"The ability of clove oil and MS-222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum)","volume":"34","author":"Wagner","year":"2003","journal-title":"Aquac. Res."},{"key":"ref_35","first-page":"151","article-title":"Influence of anticoagulants and blood storage on hematological values in tambaqui, Colossoma macropomum","volume":"20","author":"Sandrim","year":"1998","journal-title":"Acta Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105164","DOI":"10.1016\/j.rvsc.2024.105164","article-title":"Automated hematological cell count using Sysmex XN-1000V in Testudo hermanni: Agreement with manual count","volume":"169","author":"Meazzi","year":"2024","journal-title":"Res. Vet. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"International Council for Standardization in Haematology, Writing Group, Briggs, C., Culp, N., Davis, B., d\u2019Onofrio, G., Zini, G., and Machin, S.J. (2014). ICSH guidelines for the evaluation of blood cell analyzers including those used for differential leucocyte and reticulocyte counting. Int. J. Lab. Hematol., 36, 613\u2013627.","DOI":"10.1111\/ijlh.12201"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/j.1095-8649.1977.tb04140.x","article-title":"The leucocytes of fish: A review","volume":"11","author":"Ellis","year":"1977","journal-title":"J. Fish Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1080\/00365519950185328","article-title":"Preanalytical factors and their influence on analytical quality specifications","volume":"59","author":"Guder","year":"1999","journal-title":"Scand. J. Clin. Lab. Invest."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1532\/LH96.05036","article-title":"Performance evaluation of the latest fully automated hematology analyzers in a large, commercial laboratory setting: A 4-way, side-by-side study","volume":"11","author":"Bourner","year":"2005","journal-title":"Lab. Hematol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.cca.2008.07.018","article-title":"Stability of hematological analytes depends on the hematology analyzer used: A stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100","volume":"397","author":"Imeri","year":"2008","journal-title":"Clin. Chim. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.cccn.2004.02.038","article-title":"Quality specification in haematology: The automated blood cell count","volume":"346","author":"Buttarello","year":"2004","journal-title":"Clin. Chim. Acta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1939-165X.2002.tb00286.x","article-title":"Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer","volume":"31","author":"Clark","year":"2002","journal-title":"Vet. Clin. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.lab.2005.08.004","article-title":"Stability of blood cell counts, hematologic parameters and reticulocyte indexes on the Advia 120 hematology analyzer","volume":"146","author":"Lippi","year":"2005","journal-title":"J. Lab. Clin. Med."},{"key":"ref_45","first-page":"239","article-title":"Effects of two commonly used anticoagulants on hematology and erythrocyte morphology of rainbow trout (Oncorhynchus mykiss)","volume":"2","author":"Maqbool","year":"2014","journal-title":"Int. J. Fish. Aqua. Stud."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"401","DOI":"10.17221\/51\/2017-VETMED","article-title":"Stability of hematological parameters in stored blood samples of rainbow trout Oncorhynchus mykiss (Walbaum, 1792)","volume":"62","author":"Fazio","year":"2017","journal-title":"Veter. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1095-8649.1988.tb05474.x","article-title":"Effects of sampling conditions on selected blood variables of rainbow trout, Salmo gairdneri Richardson","volume":"33","author":"Korcock","year":"1988","journal-title":"J. Fish Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1939-165X.2005.tb00065.x","article-title":"Temporal effects of 3 commonly used anticoagulants on hematologic and biochemical variables in blood samples from macaws and Burmese pythons","volume":"34","author":"Harr","year":"2005","journal-title":"Vet. Clin. Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1939-165X.2012.00413.x","article-title":"ASVCP quality assurance guidelines: Control of preanalytical factors for hematology for mammalian and nonmammalian species, hemostasis and crossmatching in veterinary laboratories","volume":"41","author":"Vap","year":"2012","journal-title":"Vet. Clin. Pathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1546-5098(08)60008-4","article-title":"Fish blood cells","volume":"Volume 12","author":"Hoar","year":"1992","journal-title":"Fish Physiology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"270","DOI":"10.11606\/issn.2318-3659.v34i5p270-275","article-title":"Estudos hematol\u00f3gicos em Oreochromis niloticus (Linnaeus, 1758) (Cichlidae, Teleostei)\u2014Parte I","volume":"34","author":"Ueda","year":"1997","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s10499-018-0308-5","article-title":"The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners","volume":"27","author":"Kasprzak","year":"2019","journal-title":"Aquac. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/jfb.14746","article-title":"Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin, 1789) hematological parameters and erythrocyte fragility","volume":"99","author":"Lulijwa","year":"2021","journal-title":"J. Fish Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/cbf.2915","article-title":"Effect of storage time on hematological parameters in mullet, Mugil cephalus","volume":"31","author":"Faggio","year":"2013","journal-title":"Cell Biochem. Funct."},{"key":"ref_55","first-page":"345","article-title":"Effect of heparin and EDTA as anticoagulants on hematological values in farmed juvenile of Arapaima gigas","volume":"30","author":"Perez","year":"2022","journal-title":"Aquac. Int."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/BF00579855","article-title":"Heparin and ethylenediamine tetra-acetate as anticoagulants for fish blood","volume":"355","author":"Hattingh","year":"1975","journal-title":"Pfl\u00fcgers Arch."},{"key":"ref_57","first-page":"99","article-title":"Disodium EDTA used as anticoagulant causes hemolysis in common carp blood","volume":"35","author":"Witeska","year":"2011","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Rozas-Serri, M., Correa, R., Walker-Vergara, R., Co\u00f1uecar, D., Barrientos, S., Leiva, C., Ildefonso, R., Senn, C., and Pe\u00f1a, A. (2022). Reference intervals for blood biomarkers in farmed Atlantic salmon, Coho salmon and rainbow trout in Chile: Promoting a preventive approach in aquamedicine. Biology, 11.","DOI":"10.3390\/biology11071066"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1939-165X.2000.tb00389.x","article-title":"Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid)","volume":"29","author":"Hrubec","year":"2000","journal-title":"Vet. Clin. Pathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1095-8649.2007.01494.x","article-title":"Hematological and biochemical reference intervals for farmed channel catfish","volume":"71","author":"Moraes","year":"2007","journal-title":"J. Fish Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s10499-019-00501-3","article-title":"The influence of the endogenous and exogenous factors on hematological parameters in different fish species: A review","volume":"28","author":"Ahmed","year":"2020","journal-title":"Aquac. 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