{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:09:52Z","timestamp":1778695792619,"version":"3.51.4"},"reference-count":66,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of veterinary medicine, Kasetsart University","award":["FFK (FFK63_04)"],"award-info":[{"award-number":["FFK (FFK63_04)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Cannabidiol (CBD), a non-psychotropic cannabinoid derived from Cannabis plants, is increasingly explored for its potential therapeutic applications in veterinary medicine. This study aimed to evaluate the impact of CBD oil on the minimum alveolar concentration of isoflurane (MACiso) in cats. Sixteen healthy cats underwent isoflurane anesthesia, and the MACiso was determined using the tail-clamping technique both at baseline and 30 min after the administration of CBD oil (2 mg\/kg) via a stomach tube. CBD administration resulted in a significant 11% reduction in the MACiso, from 1.77 \u00b1 0.14% to 1.62 \u00b1 0.21% (p &lt; 0.001). Following CBD administration, heart and respiratory rates were elevated at the time of MACiso determination compared to baseline whereas other physiological parameters remained unchanged. Serum biochemical analysis conducted two weeks post administration revealed a significant decrease in blood urea nitrogen (BUN) levels while one cat exhibited a mild increase in alanine aminotransferase (ALT). Proteomic analysis identified 12 CBD-associated proteins in feline serum 30 min post administration, with CBDA and THCA synthases demonstrating significant upregulation. These findings indicate that CBD oil reduces anesthetic requirements in cats without inducing significant physiological disturbances. Further research is warranted to elucidate the underlying mechanisms of CBD\u2019s anesthetic-sparing effects and its implications in veterinary anesthesia.<\/jats:p>","DOI":"10.3390\/ani15101393","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T04:21:23Z","timestamp":1747023683000},"page":"1393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Cannabidiol Oil on Anesthetic Requirements in Cats: MAC Determination and Serum Profiling via Nanoscale Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"15","author":[{"given":"Panisara","family":"Suriyawongpongsa","sequence":"first","affiliation":[{"name":"Veterinary Clinical Studies Program, Faculty of Veterinary Medicine, Graduated School, Kasetsart University, Nakorn Pathom 73140, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirirat","family":"Niyom","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kannika","family":"Wanapinit","sequence":"additional","affiliation":[{"name":"Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monchanok","family":"Vijarnsorn","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3696-8390","authenticated-orcid":false,"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4985-2565","authenticated-orcid":false,"given":"Sekkarin","family":"Ploypetch","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.neuron.2022.12.022","article-title":"THC and CBD: Similarities and differences between siblings","volume":"111","author":"Stella","year":"2023","journal-title":"Neuron"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s40429-020-00326-8","article-title":"Therapeutic efficacy of cannabidiol (CBD): A review of the evidence from clinical trials and human laboratory studies","volume":"7","author":"Sholler","year":"2020","journal-title":"Curr. 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