{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:17:23Z","timestamp":1760059043119,"version":"build-2065373602"},"reference-count":80,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Animal Medicine, Production and Health, University of Padua","award":["2024MAPS1SIDPROGETTI-00007"],"award-info":[{"award-number":["2024MAPS1SIDPROGETTI-00007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Equid milk (donkey or mare) has traditionally been consumed raw or fermented due to its distinctive chemical composition, which closely resembles human milk, and its nutritional benefits. Nowadays, interest in it is increasing, despite limited research on its microbiological risk. Coxiella burnetii (C. burnetii), a globally re-emerging zoonotic agent, has rarely been investigated in equid milk. The objective of this study was to demonstrate the applicability of selected analytical methods for detecting C. burnetii in raw equid milk. A commercial molecular assay was tested on three serial dilutions of one donkey milk sample contaminated with a C. burnetii plasmid, which showed no interference of donkey milk with real-time PCR detection. The commercial molecular assay and a serological assay routinely used in the laboratory were also applied to screen a total of 106 equid milks from 16 farms to assess their applicability to diagnostic samples. No C. burnetii DNA or anti-C. burnetii antibodies were detected in these screenings. In our study, the implemented methods appeared to be suitable for C. burnetii investigation in equid milk. While the overall low prevalence recorded in Italy among domestic species is reassuring, strategic surveillance and risk assessment remain essential considering the low infectious dose of C. burnetii and its zoonotic potential.<\/jats:p>","DOI":"10.3390\/ani15101460","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T05:37:13Z","timestamp":1747633033000},"page":"1460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Raw Equid Milk: A Potential Risk for Q Fever?"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-8467","authenticated-orcid":false,"given":"Elisa","family":"Mazzotta","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2104-4531","authenticated-orcid":false,"given":"Alda","family":"Natale","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7809-0331","authenticated-orcid":false,"given":"Laura","family":"Bellinati","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6056-7821","authenticated-orcid":false,"given":"Letizia","family":"Ceglie","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-9024","authenticated-orcid":false,"given":"Laura","family":"Lucchese","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahsin Onur","family":"Kevenk","sequence":"additional","affiliation":[{"name":"Nova Biologicals, 1775 N Loop 336 E, Conroe, TX 77301, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3780-8604","authenticated-orcid":false,"given":"Maria Luisa","family":"Menandro","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Productions and Health, University of Padova, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Giacometti","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Productions and Health, University of Padova, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0172-3054","authenticated-orcid":false,"given":"Leonardo","family":"Alberghini","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Productions and Health, University of Padova, 35020 Legnaro, PD, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Celina, S.S., and Cern\u00fd, J. (2022). Coxiella burnetii in Ticks, Livestock, Pets and Wildlife: A Mini-Review. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.1068129"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00045-16","article-title":"From Q Fever to Coxiella burnetii Infection: A Paradigm Change","volume":"30","author":"Eldin","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Animal Health and Welfare (AHAW) (2010). Scientific Opinion on QFever. EFSA J., 8, 1595.","DOI":"10.2903\/j.efsa.2010.1595"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Alessiani, A., di Domenico, M., Averaimo, D., Pompilii, C., Rulli, M., Cocco, A., Lomellini, L., Coccaro, A., Cantelmi, M.C., and Merola, C. (2024). Coxiella burnetii: A Brief Summary of the Last Five Years of Its Presence in the Abruzzo and Molise Regions in Italy. Animals, 14.","DOI":"10.3390\/ani14152248"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Khademi, P., Ownagh, A., Ataei, B., Kazemnia, A., Enferadi, A., Khalili, M., and Mardani, K. (2020). Prevalence of C. Burnetii DNA in Sheep and Goats Milk in the Northwest of Iran. Int. J. Food Microbiol., 331.","DOI":"10.1016\/j.ijfoodmicro.2020.108716"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S1473-3099(05)70052-9","article-title":"Natural History and Pathophysiology of Q Fever","volume":"5","author":"Raoult","year":"2005","journal-title":"Lancet Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"K\u00f6rner, S., Makert, G.R., Ulbert, S., Pfeffer, M., and Mertens-Scholz, K. (2021). The Prevalence of Coxiella burnetii in Hard Ticks in Europe and Their Role in Q Fever Transmission Revisited-A Systematic Review. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.655715"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1080\/14787210.2020.1699055","article-title":"New Insights in Coxiella burnetii Infection: Diagnosis and Therapeutic Update","volume":"18","author":"Melenotte","year":"2020","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"ref_9","first-page":"e38031","article-title":"A Narrative Review of Q Fever in Europe","volume":"15","author":"Christodoulou","year":"2023","journal-title":"Cureus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1080\/01652176.2013.843809","article-title":"Clinical Microbiology of Coxiella burnetii and Relevant Aspects for the Diagnosis and Control of the Zoonotic Disease Q Fever","volume":"33","author":"Roest","year":"2013","journal-title":"Vet. Q."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.5455\/OVJ.2024.v14.i10.2","article-title":"Navigating Q Fever: Current Perspectives and Challenges in Outbreak Preparedness","volume":"14","author":"Meles","year":"2024","journal-title":"Open Vet. J."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Vincenzetti, S., Pucciarelli, S., Polzonetti, V., and Polidori, P. (2017). Role of Proteins and of Some Bioactive Peptides on the Nutritional Quality of Donkey Milk and Their Impact on Human Health. Beverages, 3.","DOI":"10.3390\/beverages3030034"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.idairyj.2011.11.008","article-title":"Equid Milk for Human Consumption","volume":"24","author":"Salimei","year":"2012","journal-title":"Int. Dairy J."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Uniacke-Lowe, T., and Fox, P.F. (2022). Equid Milk. Encyclopedia of Dairy Sciences, Elsevier.","DOI":"10.1016\/B978-0-12-818766-1.00389-5"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3168\/jdsc.2021-0175","article-title":"Valorization of Donkey Milk: Technology, Functionality, and Future Prospects","volume":"3","author":"Papademas","year":"2022","journal-title":"JDS Commun."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Doreau, M., and Martin-Rosset, W. (2011). Animals That Produce Dairy Foods: Horse. Encyclopedia of Dairy Sciences, Academic Press. [2nd ed.].","DOI":"10.1016\/B978-0-12-374407-4.00040-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"837","DOI":"10.56093\/ijans.v90i6.104972","article-title":"Donkey Milk Composition and Its Therapeutic Applications","volume":"90","author":"Bhardwaj","year":"2020","journal-title":"Indian J. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.foodcont.2014.01.045","article-title":"Consumption of Raw or Heated Milk from Different Species: An Evaluation of the Nutritional and Potential Health Benefits","volume":"42","author":"Claeys","year":"2014","journal-title":"Food Control"},{"key":"ref_19","first-page":"1125","article-title":"Mare\u2019s Milk: Composition, Properties, and Application in Medicine","volume":"76","author":"Musaev","year":"2021","journal-title":"Arch. Razi Inst."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.idairyj.2016.07.012","article-title":"Microbial Quality of Raw Horse Milk","volume":"63","author":"Hazeleger","year":"2016","journal-title":"Int. Dairy J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhou, M., Huang, F., Du, X., Wang, C., and Liu, G. (2023). Microbial Quality of Donkey Milk during Lactation Stages. Foods, 12.","DOI":"10.3390\/foods12234272"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Khan, M.Z., Chen, W., Li, M., Ren, W., Huang, B., Kou, X., Ullah, Q., Wei, L., Wang, T., and Khan, A. (2024). Is There Sufficient Evidence to Support the Health Benefits of Including Donkey Milk in the Diet?. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1404998"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"113929","DOI":"10.1016\/j.fct.2023.113929","article-title":"Recent Advances in Selective Allergies to Mammalian Milk Proteins Not Associated with Cow\u2019s Milk Proteins Allergy","volume":"178","author":"Lajnaf","year":"2023","journal-title":"Food Chem. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105541","DOI":"10.1016\/j.idairyj.2022.105541","article-title":"Thermal Processing of Equine Milk\u2014A Review","volume":"138","author":"Papademas","year":"2023","journal-title":"Int. Dairy J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.idairyj.2010.02.007","article-title":"Equine Milk Proteins: Chemistry, Structure and Nutritional Significance","volume":"20","author":"Huppertz","year":"2010","journal-title":"Int. Dairy J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1089\/vbz.2020.2764","article-title":"Prevalence and Molecular Characterization of Coxiella burnetii in Cattle, Goats, and Horses in the Republic of Korea","volume":"21","author":"Cho","year":"2021","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5782974","DOI":"10.1155\/2019\/5782974","article-title":"Potential Hazards Associated with Raw Donkey Milk Consumption: A Review","volume":"2019","author":"Conte","year":"2019","journal-title":"Int. J. Food Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.tifs.2017.11.004","article-title":"Q Fever and Prevalence of Coxiella burnetii in Milk","volume":"71","author":"Pexara","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_29","first-page":"1303","article-title":"Acute and Chronic Q Fever; Epidemiology, Symptoms, Diagnosis and Therapy of Infection Caused by Coxiella burnetii","volume":"144","author":"Bartelink","year":"2000","journal-title":"Ned. Tijdschr. Geneeskd."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Baseri, N., Omidi, A.H., Latifian, M., Mostafavi, E., Khademvatan, S., Omidifar, N., Tabaei, S.j.S., Jafari, R., Zeinali, S., and Ghasemi, A. (2024). Molecular Examination for Coxiella burnetii and Brucella Spp. Infections in Iranian Women Experiencing Spontaneous Miscarriage. BMC Infect. Dis., 24.","DOI":"10.1186\/s12879-024-09041-5"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ullah, Q., Jamil, T., Saqib, M., Iqbal, M., and Neubauer, H. (2022). Q Fever\u2014A Neglected Zoonosis. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081530"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e12444","DOI":"10.1111\/jfs.12444","article-title":"Microbiological Safety and Quality of Italian Donkey Milk","volume":"38","author":"Mottola","year":"2018","journal-title":"J. Food Saf."},{"key":"ref_33","first-page":"6306","article-title":"Guidance on Date Marking and Related Food Information: Part 1 (Date Marking)","volume":"18","author":"Koutsoumanis","year":"2020","journal-title":"EFSA J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.idairyj.2014.05.010","article-title":"A Review of the Microbiological Hazards of Raw Milk from Animal Species Other than Cows","volume":"39","author":"Verraes","year":"2014","journal-title":"Int. Dairy J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.tmaid.2007.09.039","article-title":"Isolation of Enterobacter Sakazakii from Ass\u2019 Milk in Sicily: Case Report, Safety and Legal Issues","volume":"5","author":"Conte","year":"2007","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_36","first-page":"21","article-title":"Hygienic Characteristics and Microbiological Hazard Identification in Horse and Donkey Raw Milk","volume":"52","author":"Colavita","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Chen, L., Zhao, Z.-J., and Meng, Q.-F. (2021). Detection of Specific IgG-Antibodies Against Toxoplasma Gondii in the Serum and Milk of Domestic Donkeys During Lactation in China: A Potential Public Health Concern. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.760400"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Liang, W., Zhao, S., Wang, N., Tang, Z., Zhao, F., Liu, M., Jin, W., Meng, Y., and Jia, L. (2022). Molecular Occurrence and Risk Factors for Toxoplasma Gondii Infection in Equids in Jilin, China. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-16658-6"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.rvsc.2020.07.005","article-title":"Toxoplasma Gondii Infections in Horses, Donkeys, and Other Equids: The Last Decade","volume":"132","author":"Dubey","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/zph.13026","article-title":"Seroepidemiological Study of Toxoplasma Gondii in Equids in Different European Countries","volume":"70","author":"Franco","year":"2023","journal-title":"Zoonoses Public Health"},{"key":"#cr-split#-ref_41.1","unstructured":"European Union (2002). Regulation"},{"key":"#cr-split#-ref_41.2","unstructured":"(EC) No 178\/2002 of the European Parliament and of the Council of 28 January 2002 Layingdown the General Principles and Requirements of Food Law, Establishing the European Food Safety Authorityand Laying down Procedures in Matters of Food Safet, European Union. OJ L 31."},{"key":"#cr-split#-ref_42.1","unstructured":"European Union (2004). Regulation"},{"key":"#cr-split#-ref_42.2","unstructured":"(EC) No 852\/2004 of the European Parliament and of the Council of 29 April 2004 on the Hygieneof Foodstuffs, European Union. OJ L 139."},{"key":"#cr-split#-ref_43.1","unstructured":"European Union (2004). Regulation"},{"key":"#cr-split#-ref_43.2","unstructured":"(EC) No 853\/2004 of the European Parliament and of the Council of 29 April 2004 Laying Downspecific Hygiene Rules for Food of Animal Origin, European Union. OJ L 139."},{"key":"ref_44","first-page":"406","article-title":"Regulation (EU) 2017\/625 and the \u201cUnion Agri-Food Chain Legislation\u201d: A New Comprehensive Approach in Line with the \u201cOne Health\u201d Paradigm?","volume":"12","author":"Menditto","year":"2017","journal-title":"Eur. Food Feed Law Rev."},{"key":"ref_45","unstructured":"European Union (2019). Commission Implementing Regulation (EU) 2019\/627 of 15 March 2019 Laying down Uniform Practicalarrangements for the Performance of Official Controls on Products of Animal Origin Intended for Humanconsumption in Accordance with Regulation (EU) 2017\/625 Of, European Union. OJ L 131."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5219\/legestic.1","article-title":"Overview of the Milk and Dairy Products Legislation in the European Union","volume":"1","year":"2023","journal-title":"Legestic"},{"key":"ref_47","unstructured":"European Union (2003). Directive 2003\/ 99\/ EC of the European Parliament and of the Council of 12 December 2003 on the Monitoring of Zoonoses and Zoonotic Agents, Amending Decision 90\/ 424\/ EEC and Repealing Council Directive 92\/ 117\/ EEC, European Union. OJ L 325."},{"key":"ref_48","unstructured":"World Organization for Animal Health (WOAH) (2018). Q Fever, World Organization for Animal Health (WOAH). WOAH Terrestrial Manual 2018."},{"key":"ref_49","first-page":"102","article-title":"Comparative Assessment of Toxoplasma Gondii Infection Prevalence in Romania Using 3 Serological Methods","volume":"72","author":"Gyorke","year":"2015","journal-title":"Bull. Univ. Agric. Sci. Vet. Med. Cluj-Napoca. Vet. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.vetpar.2015.07.024","article-title":"Toxoplasma Gondii in Horse Meat Intended for Human Consumption in Romania","volume":"212","author":"Rosenthal","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1292\/jvms.14-0353","article-title":"Evidence of Toxoplasma Gondii Exposure among Horses in Korea","volume":"76","author":"Lee","year":"2014","journal-title":"J. Vet. Med. Sci."},{"key":"ref_52","first-page":"131932","article-title":"Q Fever: An Old but Still a Poorly Understood Disease","volume":"2012","year":"2012","journal-title":"Interdiscip. Perspect. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"van der Kolk, J.H., and Veldhuis Kroeze, E.J.B. (2022). Infectious Diseases of the Horse, CRC Press.","DOI":"10.1201\/9781003197546"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.vetmic.2013.09.027","article-title":"Is the Horse a Reservoir or an Indicator of Coxiella burnetii Infection? Systematic Review and Biomolecular Investigation","volume":"167","author":"Marenzoni","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Khademi, P., Ownagh, A., Ataei, B., Kazemnia, A., Eydi, J., Khalili, M., Mahzounieh, M., and Mardani, K. (2020). Molecular Detection of Coxiella burnetii in Horse Sera in Iran. Comp. Immunol. Microbiol. Infect. Dis., 72.","DOI":"10.1016\/j.cimid.2020.101521"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.prevetmed.2011.11.001","article-title":"Molecular Detection of Coxiella burnetii and Neospora Caninum in Equine Aborted Foetuses and Neonates","volume":"104","author":"Leon","year":"2012","journal-title":"Prev. Vet. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1007\/s11756-021-00898-4","article-title":"First Serological Record of Coxiella burnetii Infection in the Equine Population of Slovakia","volume":"77","author":"Vojtek","year":"2022","journal-title":"Biologia"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Seo, M.-G., Lee, S.-H., VanBik, D., Ouh, I.-O., Yun, S.-H., Choi, E., Park, Y.-S., Lee, S.-E., Kim, J.W., and Cho, G.-J. (2016). Detection and Genotyping of Coxiella burnetii and Coxiella-Like Bacteria in Horses in South Korea. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156710"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Jaferi, M., Mozaffari, A., Jajarmi, M., Imani, M., and Khalili, M. (2021). Serologic and Molecular Survey of Horses to Coxiella burnetii in East of Iran a Highly Endemic Area. Comp. Immunol. Microbiol. Infect. Dis., 76.","DOI":"10.1016\/j.cimid.2021.101647"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"602","DOI":"10.54203\/scil.2020.wvj72","article-title":"Coxiella burnetii in Horses of Algeria: Seroprevalence and Associated Risk Factors","volume":"10","author":"Ansel","year":"2020","journal-title":"World\u2019s Vet. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"369","DOI":"10.4149\/av_2017_318","article-title":"Seroprevalence of Coxiella burnetii among Domestic Ruminants and Horses in Poland","volume":"61","author":"Pluta","year":"2017","journal-title":"Acta Virol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.vetmic.2017.11.012","article-title":"Seroprevalence of Horses to Coxiella burnetii in an Q Fever Endemic Area","volume":"215","author":"Desjardins","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13028-014-0046-2","article-title":"Increasing Prevalence of Coxiella burnetii Seropositive Danish Dairy Cattle Herds","volume":"56","author":"Agger","year":"2014","journal-title":"Acta Vet. Scand."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1751-0147-52-5","article-title":"Prevalence of Coxiella burnetii Antibodies in Danish Dairy Herds","volume":"52","author":"Agger","year":"2010","journal-title":"Acta Vet. Scand."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ) (2015). Scientific Opinion on the Public Health Risks Related to the Consumption of Raw Drinking Milk. EFSA J., 13, 3940.","DOI":"10.2903\/j.efsa.2015.3940"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1086\/507035","article-title":"Milk of Nonhuman Origin and Infectious Diseases in Humans","volume":"43","author":"Leedom","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s11250-015-0968-3","article-title":"High Seroprevalence of Coxiella burnetii in Dairy Cattle in China","volume":"48","author":"Kelly","year":"2016","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"de Fran\u00e7a, D.A., de Souza Ribeiro Mioni, M., Fornazari, F., de Lima Dur\u00e9, A.\u00cd., Silva, M.V.F., Possebon, F.S., Richini-Pereira, V.B., Langoni, H., and Megid, J. (2022). Seropositivity for Coxiella burnetii in Suspected Patients with Dengue in S\u00e3o Paulo State, Brazil. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010392"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"771","DOI":"10.4269\/ajtmh.14-0282","article-title":"Q Fever in French Guiana","volume":"91","author":"Eldin","year":"2014","journal-title":"Am. Soc. Trop. Med. Hyg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"100647","DOI":"10.1016\/j.focha.2024.100647","article-title":"A Comprehensive Review on Donkey Milk and Its Products: Composition, Functionality and Processing Aspects","volume":"4","author":"Meena","year":"2024","journal-title":"Food Chem. Adv."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Faccia, M., D\u2019Alessandro, A.G., Summer, A., and Hailu, Y. (2020). Milk Products from Minor Dairy Species: A Review. Animals, 10.","DOI":"10.3390\/ani10081260"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), Berezowski, J., de Balogh, K., D\u00f3rea, F.C., R\u00fcegg, S., Broglia, A., Gervelmeyer, A., and Kohnle, L. (2023). Prioritisation of Zoonotic Diseases for Coordinated Surveillance Systems under the One Health Approach for Cross-Border Pathogens That Threaten the Union. EFSA J. Eur. Food Saf. Auth., 21, e07853.","DOI":"10.2903\/j.efsa.2023.7853"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), and European Centre for Disease Prevention and Control (ECDC) (2024). The European Union One Health 2023 Zoonoses Report. EFSA J., 22, e9106.","DOI":"10.2903\/j.efsa.2024.9106"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1111\/jvim.15229","article-title":"Management of Coxiella burnetii Infection in Livestock Populations and the Associated Zoonotic Risk: A Consensus Statement","volume":"32","author":"Plummer","year":"2018","journal-title":"J. Vet. Intern. Med."},{"key":"ref_75","unstructured":"Auriemma, C., Lucibelli, M.G., Bove, F., Gallo, A., de Carlo, E., Martucciello, A., Corrado, F., and Guarino, A.G.G. (2012, January 24\u201326). Preliminary Survey on the Presence of Coxiella burnetii in Milk Samples from Donkeys Raised in Campania. Proceedings of the XIV Congresso Nazionale S.I.Di.L.V., Sorrento, NA, Italy."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/s41558-022-01426-1","article-title":"Over Half of Known Human Pathogenic Diseases Can Be Aggravated by Climate Change","volume":"12","author":"Mora","year":"2022","journal-title":"Nat. Clim. Change"},{"key":"ref_77","unstructured":"Bellinati, L., Ceglie, L., Kevenk, T.O., Lucchese, L., Natale, A., Giacometti, F., and Alberghini, L. (2024, January 21\u201323). Investigation on Q Fever agent in donkey milk. Proceedings of the EAVLD 2024\u20147th Congress of the European Association of Veterinary Laboratory Diagnosticians, Padova, PD, Italy."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/10\/1460\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:34:58Z","timestamp":1760031298000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/10\/1460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,19]]},"references-count":80,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["ani15101460"],"URL":"https:\/\/doi.org\/10.3390\/ani15101460","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,5,19]]}}}