{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:18:32Z","timestamp":1760059112799,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>It is unknown whether intestinal ultrasonographic measurements differ between lymphoplasmacytic enteritis (LPE) and low-grade intestinal T-cell lymphoma (LGITL) in cats if the diagnosis is based either on histology\/immunohistochemistry (IHC) or on clonality assay results. The effects of treatment on intestinal ultrasonographic measurements are also unknown. Therefore, we prospectively compared small intestinal wall layering between cats with LPE and LGITL and investigated whether there were differences between the groups when the diagnostic gold standard was either histology\/IHC or clonality testing. We evaluated the effects of standardized treatment in a subset of cats. The thicknesses of the total wall, mucosa, muscularis, and submucosa were measured in the duodenum, jejunum, and ileum, and ratios (muscularis to submucosa, muscularis to total wall thickness) were calculated. The thickness of the largest mesenteric lymph nodes was also determined. Ultrasonographic measurements from duodenal and jejunal segments were grouped together, and ileal segments were assessed separately. Sixteen cats with standardized full-thickness biopsies from the stomach, duodenum, jejunum, and ileum were included. Samples for clonality testing were fresh-frozen and analyzed later, and the standardized treatment was based on histologic\/IHC diagnoses. Ultrasonographic measurements were compared between LPE and LGITL when diagnoses were either based on histology\/IHC or clonality testing using a linear mixed model. Repeated ultrasonographic measurements of segments were available for seven cats after 12 weeks (five LPE, two LGITL) and five cats after 24 weeks (three LPE, two LGITL) of standardized treatment. We found that none of the ultrasonographic measurements differed between LPE and LGITL regardless of the diagnostic gold standard used. During treatment, only the ratio of lamina muscularis thickness to total wall thickness decreased significantly in LPE cats after 12 and 24 weeks compared to baseline. In conclusion, the herein evaluated ultrasonographic variables did not differ between LPE and LGITL and the diagnostic gold standard used had no influence on the results. The detected change over time during treatment in LPE cats requires further study.<\/jats:p>","DOI":"10.3390\/ani15111518","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T12:15:43Z","timestamp":1747916143000},"page":"1518","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intestinal Ultrasonographic Measurements in Cats Diagnosed with Lymphoplasmacytic Enteritis and Low-Grade T-Cell Lymphoma Based on Either Histology\/Immunohistochemistry or Clonality Testing\u2014And Assessment of the Effects of Therapy on Wall Layering After 3 and 6 Months of Treatment"],"prefix":"10.3390","volume":"15","author":[{"given":"Laura","family":"Beatrice","sequence":"first","affiliation":[{"name":"Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwei","family":"F\u00f6hr","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1594-1149","authenticated-orcid":false,"given":"Paula","family":"Grest","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3787-7952","authenticated-orcid":false,"given":"Maja","family":"Ruetten","sequence":"additional","affiliation":[{"name":"Pathovet AG, 8317 Tagelswangen, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Henrich","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Justus-Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3583-215X","authenticated-orcid":false,"given":"Simona","family":"Vincenti","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Surgery, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karolin","family":"Campbell","sequence":"additional","affiliation":[{"name":"Clinic of Diagnostic Imaging, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9492-3484","authenticated-orcid":false,"given":"Peter Hendrik","family":"Kook","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Paulin, M.V., Couronne, L., Beguin, J., Le Poder, S., Delverdier, M., Semin, M.-O., Bruneau, J., Cerf-Bensussan, N., Malamut, G., and Cellier, C. (2018). Feline low-grade alimentary lymphoma: An emerging entity and a potential animal model for human disease. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1635-5"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1111\/jvim.16690","article-title":"ACVIM consensus statement guidelines on diagnosing and distinguishing low-grade neoplastic from inflammatory lymphocytic chronic enteropathies in cats","volume":"37","author":"Marsilio","year":"2023","journal-title":"J. Vet. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1177\/1098612X14525385","article-title":"Clinicopathological and ultrasonographic features of cats with eosinophilic enteritis","volume":"16","author":"Tucker","year":"2014","journal-title":"J. Feline Med. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1111\/jvim.16231","article-title":"Histopathologic, phenotypic, and molecular criteria to discriminate low-grade intestinal T-cell lymphoma in cats from lymphoplasmacytic enteritis","volume":"35","author":"Freiche","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1177\/0300985811404712","article-title":"Feline gastrointestinal lymphoma: Mucosal architecture, immunophenotype, and molecular clonality","volume":"49","author":"Moore","year":"2012","journal-title":"Vet. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1177\/1098612X12451548","article-title":"Feline idiopathic inflammatory bowel disease: What we know and what remains to be unraveled","volume":"14","author":"Jergens","year":"2012","journal-title":"J. Feline Med. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1111\/jvim.15471","article-title":"Ability of ultrasonography to predict the presence and location of histologic lesions in the small intestine of cats","volume":"33","author":"Guttin","year":"2019","journal-title":"J. Vet. Intern. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.2460\/javma.243.10.1455","article-title":"Diagnosis of chronic small bowel disease in cats: 100 cases (2008\u20132012)","volume":"243","author":"Norsworthy","year":"2013","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1177\/1098612X19880434","article-title":"Feline abdominal ultrasonography: What\u2019s normal? What\u2019s abnormal? The diseased gastrointestinal tract","volume":"21","author":"Griffin","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"629","DOI":"10.2460\/javma.247.6.629","article-title":"Prevalence and underlying causes of histologic abnormalities in cats suspected to have chronic small bowel disease: 300 cases (2008\u20132013)","volume":"247","author":"Norsworthy","year":"2015","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1939-1676.2009.0457.x","article-title":"Ultrasonographic evaluation of the muscularis propria in cats with diffuse small intestinal lymphoma or inflammatory bowel disease","volume":"24","author":"Zwingenberger","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1177\/1098612X13498596","article-title":"Ultrasonographic thickening of the muscularis propria in feline small intestinal small cell T-cell lymphoma and inflammatory bowel disease","volume":"16","author":"Daniaux","year":"2014","journal-title":"J. Feline Med. Surg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1111\/jvim.16272","article-title":"Clinical, laboratory and ultrasonographic findings differentiating low-grade intestinal T-cell lymphoma from lymphoplasmacytic enteritis in cats","volume":"35","author":"Freiche","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1177\/0300985815626576","article-title":"Clonality testing in veterinary medicine: A review with diagnostic guidelines","volume":"53","author":"Keller","year":"2016","journal-title":"Vet. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1111\/jsap.12494","article-title":"Differentiating feline inflammatory bowel disease from alimentary lymphoma in duodenal endoscopic biopsies","volume":"57","author":"Sabattini","year":"2016","journal-title":"J. Small Anim. Pract."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/jvim.15455","article-title":"Results of histopathology, immunohistochemistry, and molecular clonality testing of small intestinal biopsy specimens from clinically healthy client-owned cats","volume":"33","author":"Marsilio","year":"2019","journal-title":"J. Vet. Intern. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2478\/raon-2013-0072","article-title":"Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays: A single institution experience","volume":"48","author":"Kokovic","year":"2014","journal-title":"Radiol. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1111\/jvim.13924","article-title":"Comparison of Serum Spec fPL and 1,2-o-Dilauryl-Rac-Glycero-3-Glutaric Acid-(6\u2032-Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology","volume":"30","author":"Oppliger","year":"2016","journal-title":"J. Vet. Intern. Med."},{"key":"ref_19","unstructured":"Mattoon, J.S., Auld, D.M., and Nyland, T.G. (2002). Small Animal Diagnostic Ultrasound, WB Saunders. [2nd ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/j.jcpa.2008.01.001","article-title":"Histopathological standards for the diagnosis of gastrointestinal inflammation in endoscopic biopsy samples from the dog and cat: A report from the World Small Animal Veterinary Association Gastrointestinal Standardization Group","volume":"138","author":"Day","year":"2008","journal-title":"J. Comp. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1939-1676.2009.0443.x","article-title":"Endoscopic, biopsy, and histopathologic guidelines for the evaluation of gastrointestinal inflammation in companion animals","volume":"24","author":"Washabau","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_22","unstructured":"Schulman, F.Y. (2002). Histological classification of hematopoietic tumors of domestic animals. World Health Organization International Histological Classification of Tumors of Domestic Animals, Armed Forces Institute of Pathology, American Registry of Pathology. [2nd ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1097\/PAI.0b013e3181845ef4","article-title":"Diagnostic utility of the B-cell lineage markers CD20, CD79a, PAX5, and CD19 in paraffin-embedded tissues from lymphoid neoplasms","volume":"17","author":"Adams","year":"2009","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.vetimm.2011.05.030","article-title":"Multiplex PCR and Genescan analysis to detect immunoglobulin heavy chain gene rearrangement in feline B-cell neoplasms","volume":"143","author":"Mochizuki","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.vetimm.2011.12.015","article-title":"GeneScan analysis to detect clonality of T-cell receptor gamma gene rearrangement in feline lymphoid neoplasms","volume":"145","author":"Mochizuki","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1177\/1098612X13509080","article-title":"Ultrasonographic measurement of the relative thickness of intestinal wall layers in clinically healthy cats","volume":"16","author":"Penninck","year":"2014","journal-title":"J. Feline Med. Surg."},{"key":"ref_27","unstructured":"R Core Team (2024). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting Linear Mixed-Effects Models using lme4","volume":"67","author":"Bates","year":"2015","journal-title":"J. Stat. Softw."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.jfms.2009.05.021","article-title":"Low-grade alimentary lymphoma: Clinicopathological findings and response to treatment in 17 cases","volume":"11","author":"Lingard","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1740-8261.2007.00320.x","article-title":"Ultrasonographic anatomy of abdominal lymph nodes in the normal cat","volume":"49","author":"Schreurs","year":"2008","journal-title":"Vet. Radiol. Ultrasoun"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1177\/0300985810389479","article-title":"Diagnostic algorithm to differentiate lymphoma from inflammation in feline small intestinal biopsy samples","volume":"48","author":"Kiupel","year":"2011","journal-title":"Vet. Pathol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/11\/1518\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:38:41Z","timestamp":1760031521000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/15\/11\/1518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,22]]},"references-count":31,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["ani15111518"],"URL":"https:\/\/doi.org\/10.3390\/ani15111518","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2025,5,22]]}}}